MD07G1130400.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002430
Physical Location & Seq
Reverse (-)
18640380 .. 18642486
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1130400.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCCGACGAGAAGAACGCCGGATCGCCGGTGGAGCACGAGCCATCGCTGATGGAGAAGATCCACGGCGGCGATCACGATTCCTCGTTGTCGTCTTCTTCCGACTCGGATAACGAGAAATCGGAGAAGAAGGCCGAGTCTGTGAAGACCAAGGTCTTCCGGATTTTTGGCCGGGAGAAGCCGGTCCACCATGTGCTCGGCGGTGGAAAGACTGCTGATGTATTTTTGTGGAGAAACAAGAAGGTTTCGGGTGGAGTTCTTGGCGGAGCCACCGTAGTCTGGATCCTTTTTGAGTTGCTTGAGTACAACCTGCTTACTCTTGTTGCCCACATTTTGATTGCCACCCTTGCAATCTTGTTCCTCTGGTCCAATGCCCATACTTTTATCCACAAGACTCCTCCTCGTATTCCAGAAGTTCGTCTTCCTGAAGAACCATTCCTGCAGGTTGCATCTGAGCTGAGAGTTGTAATCAACTGCGCTTTTGCACTCCTTCACAATATTGCATCTGGGAAAGACTTGAAGAAGTTCCTTGCTGTTATTGCTGGCCTGTGGGTTCTTTCCGTTGTGGGCAAATGGTGTAACTTCCTGACCTTGTTTTACATATCCTTTGTTTTGCTGCACACCGTGCCCGTCATTTATGAGAAGTACGAAGACAAAATCGACCCACTTGCGGAGAAGGTGACAATCGAATTCAAGAAGCAGTATGCCGTGTTTGATGCCAAGGTTTTGAATAAGATTCCGCGTGGTCTGAAAAACAAGAGGGCTTAG

Protein Analysis

256

Amino Acids

28.55

Weight (kDa)

9.04

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 72 - 227 2.5e-60 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 318, 433
AccII CGCG 1 cut(s) 742
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 5 cut(s) 69, 201, 264, 671, 740
AclWI GGATC 4 cut(s) 31, 55, 277, 290
AcoI YGGCCR 2 cut(s) 3, 169
AcsI RAATTY 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 447
AdeI CACNNNGTG 2 cut(s) 193, 625
AfaI GTAC 2 cut(s) 305, 647
AfiI CCNNNNNNNGG 2 cut(s) 279, 670
AgsI TTSAA 3 cut(s) 520, 694, 730
AluBI AGCT 1 cut(s) 457
AluI AGCT 1 cut(s) 457
Alw21I GWGCWC 2 cut(s) 39, 198
AlwI GGATC 4 cut(s) 31, 55, 277, 290
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 4 cut(s) 3, 132, 169, 544
ApeKI GCWGC 1 cut(s) 616
ApoI RAATTY 1 cut(s) 689
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 184, 366
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 691
AvaII GGWCC 2 cut(s) 184, 366
BaeGI GKGCMC 1 cut(s) 630
BamHI GGATCC 1 cut(s) 282
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 5 cut(s) 87, 148, 152, 413, 657
Bbv12I GWGCWC 2 cut(s) 39, 198
BbvI GCAGC 1 cut(s) 603
BccI CCATC 2 cut(s) 46, 52
BceAI ACGGC 2 cut(s) 82, 692
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BcnI CCSGG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 440
BfuAI ACCTGC 2 cut(s) 318, 433
BisI GCNGC 2 cut(s) 70, 617
BlsI GCNGC 2 cut(s) 71, 618
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 2 cut(s) 184, 366
BmgT120I GGNCC 2 cut(s) 184, 366
BmiI GGNNCC 2 cut(s) 268, 284
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 3 cut(s) 458, 512, 706
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 5 cut(s) 87, 148, 152, 413, 657
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 64, 150, 720
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 670
Bse118I RCCGGY 2 cut(s) 28, 181
BseAI TCCGGA 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 64, 150, 720
BseLI CCNNNNNNNGG 2 cut(s) 279, 670
BseMII CTCAG 2 cut(s) 444, 449
BseRI GAGGAG 2 cut(s) 387, 390
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 1 cut(s) 603
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 4 cut(s) 5, 134, 171, 546
BsiHKAI GWGCWC 2 cut(s) 39, 198
BsiSI CCGG 5 cut(s) 21, 29, 160, 172, 182
BslI CCNNNNNNNGG 2 cut(s) 279, 670
BsnI GGCC 4 cut(s) 5, 134, 171, 546
Bsp1286I GDGCHC 3 cut(s) 39, 198, 630
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 4 cut(s) 23, 60, 73, 282
BspACI CCGC 5 cut(s) 69, 201, 264, 671, 740
BspANI GGCC 4 cut(s) 5, 134, 171, 546
BspCNI CTCAG 2 cut(s) 445, 450
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 2 cut(s) 268, 284
BspMAI CTGCAG 1 cut(s) 444
BspMI ACCTGC 2 cut(s) 318, 433
BspPI GGATC 4 cut(s) 31, 55, 277, 290
BsrFI RCCGGY 2 cut(s) 28, 181
BssAI RCCGGY 2 cut(s) 28, 181
BssECI CCNNGG 3 cut(s) 64, 150, 720
BssMI GATC 4 cut(s) 23, 60, 73, 282
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 150, 720
Bst2BI CACGAG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 274, 625
BstAPI GCANNNNNTGC 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 3 cut(s) 453, 458, 765
BstDSI CCRYGG 1 cut(s) 64
BstFNI CGCG 1 cut(s) 742
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 4 cut(s) 26, 63, 76, 285
BstMBI GATC 4 cut(s) 23, 60, 73, 282
BstMWI GCNNNNNNNGC 4 cut(s) 34, 347, 539, 625
BstPAI GACNNNNGTC 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 630
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 5 cut(s) 87, 148, 152, 413, 657
BstX2I RGATCY 2 cut(s) 60, 282
BstYI RGATCY 2 cut(s) 60, 282
BsuRI GGCC 4 cut(s) 5, 134, 171, 546
BtgI CCRYGG 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 30
BveI ACCTGC 2 cut(s) 318, 433
Cac8I GCNNGC 1 cut(s) 544
CfoI GCGC 1 cut(s) 479
Cfr10I RCCGGY 2 cut(s) 28, 181
Cfr13I GGNCC 2 cut(s) 184, 366
Csp6I GTAC 2 cut(s) 304, 646
CviAII CATG 1 cut(s) 191
CviJI RGCY 9 cut(s) 5, 43, 134, 171, 181, 269, 457, 546, 764
CviKI_1 RGCY 9 cut(s) 5, 43, 134, 171, 181, 269, 457, 546, 764
CviQI GTAC 2 cut(s) 304, 646
DdeI CTNAG 3 cut(s) 453, 458, 765
DpnI GATC 4 cut(s) 25, 62, 75, 284
DpnII GATC 4 cut(s) 23, 60, 73, 282
DraIII CACNNNGTG 2 cut(s) 193, 625
EaeI YGGCCR 2 cut(s) 3, 169
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 2 cut(s) 150, 720
Eco47I GGWCC 2 cut(s) 184, 366
Eco57I CTGAAG 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 689
EcoT14I CCWWGG 2 cut(s) 150, 720
ErhI CCWWGG 2 cut(s) 150, 720
FaeI CATG 1 cut(s) 194
FaiI YATR 5 cut(s) 192, 378, 602, 639, 705
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 70, 617
Fsp4HI GCNGC 2 cut(s) 70, 617
GlaI GCGC 1 cut(s) 478
GluI GCNGC 2 cut(s) 70, 617
HaeIII GGCC 4 cut(s) 5, 134, 171, 546
HapII CCGG 5 cut(s) 21, 29, 160, 172, 182
HhaI GCGC 1 cut(s) 479
Hin1II CATG 1 cut(s) 194
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HinfI GANTC 5 cut(s) 80, 104, 137, 394, 736
HpaII CCGG 5 cut(s) 21, 29, 160, 172, 182
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 5 cut(s) 103, 109, 124, 454, 750
Hpy188III TCNNGA 7 cut(s) 77, 160, 280, 410, 425, 586, 694
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 124, 235, 500, 670
HpyCH4III ACNGT 2 cut(s) 274, 625
HpyCH4V TGCA 6 cut(s) 350, 442, 449, 485, 503, 619
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 347, 539, 625
HpyF3I CTNAG 3 cut(s) 453, 458, 765
Hsp92II CATG 1 cut(s) 194
HspAI GCGC 1 cut(s) 477
Kpn2I TCCGGA 1 cut(s) 159
Kzo9I GATC 4 cut(s) 23, 60, 73, 282
LmnI GCTCC 2 cut(s) 34, 266
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 3 cut(s) 458, 512, 706
MaeIII GTNAC 2 cut(s) 578, 679
MalI GATC 4 cut(s) 25, 62, 75, 284
MboI GATC 4 cut(s) 23, 60, 73, 282
MflI RGATCY 2 cut(s) 60, 282
MhlI GDGCHC 3 cut(s) 39, 198, 630
MluCI AATT 1 cut(s) 689
MlyI GAGTC 3 cut(s) 98, 146, 388
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 5 cut(s) 94, 371, 408, 411, 753
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 524
MspI CCGG 5 cut(s) 21, 29, 160, 172, 182
MspR9I CCNGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 4 cut(s) 34, 347, 539, 625
NciI CCSGG 1 cut(s) 173
NdeII GATC 4 cut(s) 23, 60, 73, 282
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 2 cut(s) 268, 284
NmeAIII GCCGAG 2 cut(s) 160, 177
NmuCI GTSAC 1 cut(s) 679
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 2 cut(s) 80, 736
PkrI GCNGC 2 cut(s) 71, 618
PleI GAGTC 3 cut(s) 98, 145, 388
PpsI GAGTC 3 cut(s) 98, 145, 388
PshAI GACNNNNGTC 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 268, 284
PspPI GGNCC 2 cut(s) 184, 366
PstI CTGCAG 1 cut(s) 444
PsuI RGATCY 2 cut(s) 60, 282
RsaI GTAC 2 cut(s) 305, 647
RsaNI GTAC 2 cut(s) 304, 646
SatI GCNGC 2 cut(s) 70, 617
Sau3AI GATC 4 cut(s) 23, 60, 73, 282
Sau96I GGNCC 2 cut(s) 184, 366
SbfI CCTGCAGG 1 cut(s) 444
SchI GAGTC 3 cut(s) 98, 146, 388
ScrFI CCNGG 1 cut(s) 173
SdaI CCTGCAGG 1 cut(s) 444
SduI GDGCHC 3 cut(s) 39, 198, 630
SetI ASST 8 cut(s) 156, 246, 312, 447, 459, 593, 681, 726
SfaNI GCATC 3 cut(s) 458, 512, 706
SfcI CTRYAG 1 cut(s) 440
SgrAI CRCCGGYG 1 cut(s) 28
SinI GGWCC 2 cut(s) 184, 366
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse8387I CCTGCAGG 1 cut(s) 444
Sse9I AATT 1 cut(s) 689
SsiI CCGC 5 cut(s) 69, 201, 264, 671, 740
SspI AATATT 1 cut(s) 499
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 2 cut(s) 150, 720
TaaI ACNGT 2 cut(s) 274, 625
TaqI TCGA 2 cut(s) 660, 687
TasI AATT 1 cut(s) 689
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 2 cut(s) 80, 736
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 1 cut(s) 616
Tsp45I GTSAC 1 cut(s) 679
TspGWI ACGGA 1 cut(s) 550
VpaK11BI GGWCC 2 cut(s) 184, 366
XapI RAATTY 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.