RchiOBHm_Chr1g0357851

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002430
Physical Location & Seq
Forward (+)
50083793 .. 50086024
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58306

Sequence Viewer

Length: 783 bp
ATGGCCGAGCACAGTGAGAGCGCCGATGCGAAGGTGGAGCAGACGATGATGGACAAGATCAGCGAGAAGATCCACGATCATCACGACTCTTCGTCGTCGTCGTCTTCGGATTCCGACACCGAGAAGCCGGCGCCGGCCACGGAGGAGTCGTCGGTCAAGGAAAAGGTGTTCCGTCTCTTCGGAAGGGAGCGTCCTGTGCATCATGTCTTTGGCGGTGGAAAGCCTGCTGATGTGTTTTTGTGGAGGAACAAGAAAATTTCAGCTGGGGTTCTTGGAGGAGCTACTGCTGTTTATGTCCTTTTTGAGTTGCTTCAATACCACTTGCTTACGCTTATTTGCCACATTCTGGTTGGTACCCTAGCAATCTTGTTTCTGTGGTCCAATGCCCATACTATTATCCACAAGACTCCACCGCGTATCCCAGAGGTTTGTCTTCCTGAGGAGCCATTCCTGCAGGTTGCTTCTGAGCTGAGACATCAAATCAACTGGACTTTTGCACAACTTCATAACATTGCATCTGGAAGGGAGTTGAAGAAATTCCTGATTGTTATTGCTGGCTTGTGGGTTCTTTCCATTGTTGGGAGTTGGTGCAATTTCTTGACCTTGTTTTACATTACTTTTGTTTTGCTGCACACTGTGCCTGTTCTGTATGAGAAGTATGAACACAAGGTTGACCCACTTGCAGAGAAGGCAACAGTTGAGATCAAAAAGCAGTATGGAGTGTTTGATGCCAAGGTTTTGACAAAGGTTCGCGAAACAGTGCTGAAAAACAAGAGATCTTAG

Protein Analysis

260

Amino Acids

29.39

Weight (kDa)

7.81

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 76 - 231 3.2e-57 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 445
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 130, 353
AccB7I CCANNNNNTGG 1 cut(s) 346
AccII CGCG 2 cut(s) 415, 753
AciI CCGC 2 cut(s) 213, 413
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 2 cut(s) 3, 135
AcsI RAATTY 2 cut(s) 255, 536
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 2 cut(s) 346, 579
AgsI TTSAA 2 cut(s) 314, 532
AhdI GACNNNNNGTC 1 cut(s) 91
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 263, 281, 469
AluI AGCT 3 cut(s) 263, 281, 469
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 466
AlwI GGATC 1 cut(s) 64
AoxI GGCC 2 cut(s) 3, 135
ApeKI GCWGC 1 cut(s) 628
ApoI RAATTY 2 cut(s) 255, 536
Asp700I GAANNNNTTC 1 cut(s) 536
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 23, 133
AspS9I GGNCC 1 cut(s) 378
AvaII GGWCC 1 cut(s) 378
AxyI CCTNAGG 1 cut(s) 438
BanI GGYRCC 2 cut(s) 130, 353
BbsI GAAGAC 2 cut(s) 96, 425
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 615
BccI CCATC 1 cut(s) 43
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 179, 466
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 2 cut(s) 24, 134
BfuAI ACCTGC 1 cut(s) 445
BfuI GTATCC 1 cut(s) 428
BglII AGATCT 1 cut(s) 776
BisI GCNGC 1 cut(s) 629
BlsI GCNGC 1 cut(s) 630
Bme18I GGWCC 1 cut(s) 378
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 3 cut(s) 132, 355, 444
BmsI GCATC 4 cut(s) 16, 208, 524, 718
BpiI GAAGAC 2 cut(s) 96, 425
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 138, 732
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 579
Bse118I RCCGGY 2 cut(s) 127, 133
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 138, 732
BseLI CCNNNNNNNGG 2 cut(s) 346, 579
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 3 cut(s) 429, 456, 461
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 3 cut(s) 158, 291, 455
BseXI GCAGC 1 cut(s) 615
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 2 cut(s) 415, 753
BshFI GGCC 2 cut(s) 5, 137
BshNI GGYRCC 2 cut(s) 130, 353
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 128, 134
BslI CCNNNNNNNGG 2 cut(s) 346, 579
BsmAI GTCTC 2 cut(s) 179, 466
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 5, 137
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 5 cut(s) 57, 69, 76, 702, 776
Bsp68I TCGCGA 1 cut(s) 753
BspACI CCGC 2 cut(s) 213, 413
BspANI GGCC 2 cut(s) 5, 137
BspCNI CTCAG 3 cut(s) 430, 457, 462
BspFNI CGCG 2 cut(s) 415, 753
BspLI GGNNCC 3 cut(s) 132, 355, 444
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 2 cut(s) 130, 353
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 2 cut(s) 127, 133
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 2 cut(s) 127, 133
BssECI CCNNGG 2 cut(s) 138, 732
BssMI GATC 5 cut(s) 57, 69, 76, 702, 776
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 732
Bst4CI ACNGT 4 cut(s) 14, 637, 697, 760
Bst6I CTCTTC 2 cut(s) 94, 182
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 4 cut(s) 129, 135, 225, 556
BstDEI CTNAG 4 cut(s) 438, 465, 470, 780
BstDSI CCRYGG 1 cut(s) 138
BstFNI CGCG 2 cut(s) 415, 753
BstH2I RGCGCY 2 cut(s) 24, 134
BstHHI GCGC 2 cut(s) 23, 133
BstKTI GATC 5 cut(s) 60, 72, 79, 705, 779
BstMAI GTCTC 2 cut(s) 179, 466
BstMBI GATC 5 cut(s) 57, 69, 76, 702, 776
BstMWI GCNNNNNNNGC 4 cut(s) 196, 451, 637, 689
BstSFI CTRYAG 1 cut(s) 452
BstUI CGCG 2 cut(s) 415, 753
BstV1I GCAGC 1 cut(s) 615
BstV2I GAAGAC 2 cut(s) 96, 425
BstX2I RGATCY 2 cut(s) 69, 776
BstYI RGATCY 2 cut(s) 69, 776
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 5, 137
BtgI CCRYGG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 19, 633, 765
BtuMI TCGCGA 1 cut(s) 753
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 4 cut(s) 129, 135, 225, 556
CfoI GCGC 2 cut(s) 23, 133
Cfr10I RCCGGY 2 cut(s) 127, 133
Cfr13I GGNCC 1 cut(s) 378
CseI GACGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 354
CviAII CATG 1 cut(s) 203
CviJI RGCY 9 cut(s) 5, 127, 137, 223, 263, 281, 445, 469, 558
CviKI_1 RGCY 9 cut(s) 5, 127, 137, 223, 263, 281, 445, 469, 558
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 4 cut(s) 438, 465, 470, 780
DinI GGCGCC 1 cut(s) 132
DpnI GATC 5 cut(s) 59, 71, 78, 704, 778
DpnII GATC 5 cut(s) 57, 69, 76, 702, 776
DraIII CACNNNGTG 1 cut(s) 637
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 2 cut(s) 3, 135
Eam1104I CTCTTC 2 cut(s) 94, 182
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 2 cut(s) 94, 182
Eco130I CCWWGG 1 cut(s) 732
Eco47I GGWCC 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 732
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 732
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 1 cut(s) 206
FaiI YATR 7 cut(s) 204, 294, 390, 507, 651, 660, 717
FatI CATG 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 629
Fsp4HI GCNGC 1 cut(s) 629
FspBI CTAG 1 cut(s) 359
GlaI GCGC 2 cut(s) 22, 132
GluI GCNGC 1 cut(s) 629
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 24, 134
HaeIII GGCC 2 cut(s) 5, 137
HapII CCGG 2 cut(s) 128, 134
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 23, 133
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 2 cut(s) 21, 131
HinP1I GCGC 2 cut(s) 21, 131
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 4 cut(s) 86, 110, 146, 406
HpaII CCGG 2 cut(s) 128, 134
Hpy166II GTNNAC 1 cut(s) 673
Hpy188I TCNGA 4 cut(s) 109, 115, 182, 466
Hpy188III TCNNGA 6 cut(s) 83, 437, 519, 541, 598, 752
Hpy8I GTNNAC 1 cut(s) 673
Hpy99I CGWCG 4 cut(s) 97, 100, 103, 154
HpyAV CCTTC 4 cut(s) 25, 177, 516, 682
HpyCH4III ACNGT 4 cut(s) 14, 637, 697, 760
HpyCH4V TGCA 7 cut(s) 199, 454, 497, 515, 591, 631, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 451, 637, 689
HpyF3I CTNAG 4 cut(s) 438, 465, 470, 780
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 2 cut(s) 21, 131
KasI GGCGCC 1 cut(s) 130
KpnI GGTACC 1 cut(s) 357
KroI GCCGGC 2 cut(s) 127, 133
KroNI GCCGGC 2 cut(s) 129, 135
Kzo9I GATC 5 cut(s) 57, 69, 76, 702, 776
LmnI GCTCC 4 cut(s) 37, 187, 278, 442
Lsp1109I GCAGC 1 cut(s) 615
LweI GCATC 4 cut(s) 16, 208, 524, 718
MaeI CTAG 1 cut(s) 359
MalI GATC 5 cut(s) 59, 71, 78, 704, 778
MboI GATC 5 cut(s) 57, 69, 76, 702, 776
MboII GAAGA 6 cut(s) 79, 81, 96, 169, 425, 544
MflI RGATCY 2 cut(s) 69, 776
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 3 cut(s) 255, 536, 592
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 3 cut(s) 80, 155, 400
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 136, 237, 269, 418, 433
MroNI GCCGGC 2 cut(s) 127, 133
MroXI GAANNNNTTC 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 128, 134
MvnI CGCG 2 cut(s) 415, 753
MwoI GCNNNNNNNGC 4 cut(s) 196, 451, 637, 689
NaeI GCCGGC 2 cut(s) 129, 135
NarI GGCGCC 1 cut(s) 131
NdeII GATC 5 cut(s) 57, 69, 76, 702, 776
NgoMIV GCCGGC 2 cut(s) 127, 133
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 132, 355, 444
NmeAIII GCCGAG 1 cut(s) 31
NruI TCGCGA 1 cut(s) 753
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 98, 146
PdiI GCCGGC 2 cut(s) 129, 135
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 1 cut(s) 630
PleI GAGTC 3 cut(s) 80, 154, 400
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 3 cut(s) 80, 154, 400
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 132, 355, 444
PspPI GGNCC 1 cut(s) 378
PstI CTGCAG 1 cut(s) 456
PsuI RGATCY 2 cut(s) 69, 776
PvuII CAGCTG 1 cut(s) 263
RruI TCGCGA 1 cut(s) 753
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SatI GCNGC 1 cut(s) 629
Sau3AI GATC 5 cut(s) 57, 69, 76, 702, 776
Sau96I GGNCC 1 cut(s) 378
SbfI CCTGCAGG 1 cut(s) 456
SchI GAGTC 3 cut(s) 80, 155, 400
SdaI CCTGCAGG 1 cut(s) 456
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 4 cut(s) 16, 208, 524, 718
SfcI CTRYAG 1 cut(s) 452
SfoI GGCGCC 1 cut(s) 132
SinI GGWCC 1 cut(s) 378
Sse8387I CCTGCAGG 1 cut(s) 456
Sse9I AATT 3 cut(s) 255, 536, 592
SsiI CCGC 2 cut(s) 213, 413
SspDI GGCGCC 1 cut(s) 130
SspMI CTAG 1 cut(s) 359
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 4 cut(s) 14, 637, 697, 760
TaqII GACCGA 1 cut(s) 142
TasI AATT 3 cut(s) 255, 536, 592
TfiI GAWTC 1 cut(s) 110
TscAI CASTG 3 cut(s) 19, 640, 765
TseI GCWGC 1 cut(s) 628
TspDTI ATGAA 2 cut(s) 494, 675
TspGWI ACGGA 2 cut(s) 155, 161
TspRI CASTG 3 cut(s) 19, 640, 765
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 2 cut(s) 255, 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.