MD01G1072500.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002430
Physical Location & Seq
Forward (+)
17775991 .. 17778276
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1072500.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGCCGATGAGAAGGAACACTCCGGATCGACGGTGGAGGATGAGCCATCCCTGTTGGAGAAGATGGGCGACAAGATCCACGGCGGTGATCACCATTCGTCCTCGTCCTCTTCTTCCGACTCGGATAACGAGAAATCGGAGAAGAAGACCGAGTCGGTGAAGGCCAAGGTTTTCCGGATTTTCGGCCGTGAGAGGCCGGTTCACCATGTGCTCGGCGGTGGAAAGCCTGCTGATGTGTTCTTGTGGAGAAACAAGAAGGTTTCGGCTGGAGTTCTCAGCGGAGCCACCGTCATCTGGGTCCTCTTCGAGTTGCTTCAGTACCACCTGCTTACACTTGTTGGCCACATTCTGATTGGTGCCCTTGCAATCTTGTTCCTCTGGTCCAATGCCCATGCTTTTATCCACAAGACTCCTCCTCGTATCCCTGAAGTTCGCCTTCCTGAAGAACCATTCCTGCAGGTTGCATCTGAGCTGAGAGTTCAAATCAACTGTGCATTTGCACTGCTTCATGATATTGCATCTGGGAGAGACTTGAAGAAATTCCTCGCTGTTATTGCTGGCTTGTGGGTTCTATCCGTTGTGGGCAAATGGTGCAACTTCCTGACCTTGTTTTACATATGCTTTGTTGTGCTGCACACCGTGCCCGTCATTTATGAGAAGTACGAAGACAAGATCGACCCACTCGCAGAGAGGGCGACTATCGAGTTCAAAAAGCAGTATGCCGTGTTTGATGCCAAGGTTTTGAGTAAGATTCCGCATGGTCTAAAAAACAAGAGGTCTTAG

Protein Analysis

261

Amino Acids

29.15

Weight (kDa)

8.63

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 77 - 231 3.1e-59 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 333
Acc36I ACCTGC 2 cut(s) 333, 448
AccB1I GGYRCC 1 cut(s) 356
AccIII TCCGGA 2 cut(s) 23, 174
AciI CCGC 4 cut(s) 84, 216, 279, 755
AclWI GGATC 2 cut(s) 34, 70
AcoI YGGCCR 3 cut(s) 3, 184, 340
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 3 cut(s) 299, 447, 462
AdeI CACNNNGTG 2 cut(s) 208, 640
AfaI GTAC 2 cut(s) 320, 662
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 3 cut(s) 482, 535, 709
AleI CACNNNNGTG 1 cut(s) 84
AluBI AGCT 1 cut(s) 472
AluI AGCT 1 cut(s) 472
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 2 cut(s) 34, 70
Aor13HI TCCGGA 2 cut(s) 23, 174
AoxI GGCC 5 cut(s) 3, 162, 184, 194, 340
ApeKI GCWGC 1 cut(s) 631
ApoI RAATTY 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 298, 381
AsuHPI GGTGA 4 cut(s) 83, 98, 169, 194
AvaII GGWCC 2 cut(s) 298, 381
BaeGI GKGCMC 2 cut(s) 361, 645
BalI TGGCCA 1 cut(s) 342
BanI GGYRCC 1 cut(s) 356
BbsI GAAGAC 2 cut(s) 152, 672
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 618
BccI CCATC 2 cut(s) 55, 58
BceAI ACGGC 3 cut(s) 97, 171, 707
BciVI GTATCC 1 cut(s) 431
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 455
BfuAI ACCTGC 2 cut(s) 333, 448
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 1 cut(s) 632
BlsI GCNGC 1 cut(s) 633
Bme18I GGWCC 2 cut(s) 298, 381
BmgT120I GGNCC 2 cut(s) 298, 381
BmiI GGNNCC 3 cut(s) 283, 299, 358
BmsI GCATC 3 cut(s) 473, 527, 721
BpiI GAAGAC 2 cut(s) 152, 672
BpmI CTGGAG 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 79, 165, 735
BsaWI WCCGGW 2 cut(s) 23, 174
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 196
BseAI TCCGGA 2 cut(s) 23, 174
BseDI CCNNGG 3 cut(s) 79, 165, 735
BseGI GGATG 2 cut(s) 46, 47
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 3 cut(s) 289, 459, 464
BseRI GAGGAG 2 cut(s) 402, 405
BseSI GKGCMC 2 cut(s) 361, 645
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 618
BsgI GTGCAG 1 cut(s) 617
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 5 cut(s) 5, 164, 186, 196, 342
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 3 cut(s) 24, 175, 197
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 522
BsnI GGCC 5 cut(s) 5, 164, 186, 196, 342
Bsp1286I GDGCHC 3 cut(s) 213, 361, 645
Bsp13I TCCGGA 2 cut(s) 23, 174
Bsp143I GATC 4 cut(s) 26, 75, 88, 672
BspACI CCGC 4 cut(s) 84, 216, 279, 755
BspANI GGCC 5 cut(s) 5, 164, 186, 196, 342
BspCNI CTCAG 3 cut(s) 288, 460, 465
BspEI TCCGGA 2 cut(s) 23, 174
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 283, 299, 358
BspMAI CTGCAG 1 cut(s) 459
BspMI ACCTGC 2 cut(s) 333, 448
BspPI GGATC 2 cut(s) 34, 70
BspT107I GGYRCC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 3 cut(s) 79, 165, 735
BssMI GATC 4 cut(s) 26, 75, 88, 672
BssT1I CCWWGG 2 cut(s) 165, 735
Bst4CI ACNGT 4 cut(s) 34, 289, 491, 640
Bst6I CTCTTC 2 cut(s) 115, 308
BstAPI GCANNNNNTGC 2 cut(s) 591, 640
BstC8I GCNNGC 2 cut(s) 228, 559
BstDEI CTNAG 4 cut(s) 275, 468, 473, 780
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 46, 47
BstKTI GATC 4 cut(s) 29, 78, 91, 675
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 4 cut(s) 26, 75, 88, 672
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
BstSFI CTRYAG 1 cut(s) 455
BstSLI GKGCMC 2 cut(s) 361, 645
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 2 cut(s) 152, 672
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BstZI CGGCCG 1 cut(s) 184
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 5 cut(s) 5, 164, 186, 196, 342
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 46, 47
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 1 cut(s) 500
BveI ACCTGC 2 cut(s) 333, 448
Cac8I GCNNGC 2 cut(s) 228, 559
CciI TCATGA 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 298, 381
Csp6I GTAC 2 cut(s) 319, 661
CviAII CATG 4 cut(s) 206, 392, 509, 758
CviQI GTAC 2 cut(s) 319, 661
DdeI CTNAG 4 cut(s) 275, 468, 473, 780
DpnI GATC 4 cut(s) 28, 77, 90, 674
DpnII GATC 4 cut(s) 26, 75, 88, 672
DraIII CACNNNGTG 2 cut(s) 208, 640
EaeI YGGCCR 3 cut(s) 3, 184, 340
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 115, 308
EarI CTCTTC 2 cut(s) 115, 308
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 165, 735
Eco47I GGWCC 2 cut(s) 298, 381
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 3 cut(s) 299, 447, 462
EcoO109I RGGNCCY 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 165, 735
ErhI CCWWGG 2 cut(s) 165, 735
FaeI CATG 4 cut(s) 209, 395, 512, 761
FaiI YATR 8 cut(s) 207, 393, 510, 617, 619, 654, 720, 759
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 4 cut(s) 205, 391, 508, 757
FauNDI CATATG 1 cut(s) 617
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 632
FokI GGATG 2 cut(s) 34, 53
Fsp4HI GCNGC 1 cut(s) 632
GluI GCNGC 1 cut(s) 632
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 5 cut(s) 5, 164, 186, 196, 342
HapII CCGG 3 cut(s) 24, 175, 197
Hin1II CATG 4 cut(s) 209, 395, 512, 761
HinfI GANTC 4 cut(s) 119, 152, 409, 751
HpaII CCGG 3 cut(s) 24, 175, 197
HphI GGTGA 4 cut(s) 83, 98, 169, 194
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 5 cut(s) 118, 124, 139, 351, 469
Hpy188III TCNNGA 5 cut(s) 24, 175, 440, 509, 601
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 4 cut(s) 7, 154, 250, 446
HpyCH4III ACNGT 4 cut(s) 34, 289, 491, 640
HpyCH4V TGCA 8 cut(s) 365, 457, 464, 494, 500, 518, 594, 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
HpyF3I CTNAG 4 cut(s) 275, 468, 473, 780
Hsp92II CATG 4 cut(s) 209, 395, 512, 761
Kpn2I TCCGGA 2 cut(s) 23, 174
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 4 cut(s) 26, 75, 88, 672
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 3 cut(s) 473, 527, 721
MalI GATC 4 cut(s) 28, 77, 90, 674
MboI GATC 4 cut(s) 26, 75, 88, 672
MboII GAAGA 9 cut(s) 73, 102, 105, 154, 157, 295, 455, 547, 677
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 3 cut(s) 213, 361, 645
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 1 cut(s) 539
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 3 cut(s) 113, 161, 403
MmeI TCCRAC 2 cut(s) 36, 141
Mox20I TGGCCA 1 cut(s) 342
MroI TCCGGA 2 cut(s) 23, 174
MroXI GAANNNNTTC 1 cut(s) 539
MscI TGGCCA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 3 cut(s) 24, 175, 197
MwoI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
NdeI CATATG 1 cut(s) 617
NdeII GATC 4 cut(s) 26, 75, 88, 672
NlaIII CATG 4 cut(s) 209, 395, 512, 761
NlaIV GGNNCC 3 cut(s) 283, 299, 358
NmeAIII GCCGAG 1 cut(s) 192
OliI CACNNNNGTG 1 cut(s) 84
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 539
PfeI GAWTC 1 cut(s) 751
PflFI GACNNNGTC 1 cut(s) 151
PkrI GCNGC 1 cut(s) 633
PleI GAGTC 3 cut(s) 113, 160, 403
PpsI GAGTC 3 cut(s) 113, 160, 403
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 283, 299, 358
PspPI GGNCC 2 cut(s) 298, 381
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 1 cut(s) 459
PsuI RGATCY 1 cut(s) 75
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 2 cut(s) 320, 662
RsaNI GTAC 2 cut(s) 319, 661
RseI CAYNNNNRTG 1 cut(s) 84
SatI GCNGC 1 cut(s) 632
Sau3AI GATC 4 cut(s) 26, 75, 88, 672
Sau96I GGNCC 2 cut(s) 298, 381
SbfI CCTGCAGG 1 cut(s) 459
SchI GAGTC 3 cut(s) 113, 161, 403
SdaI CCTGCAGG 1 cut(s) 459
SduI GDGCHC 3 cut(s) 213, 361, 645
SetI ASST 8 cut(s) 171, 261, 327, 462, 474, 608, 741, 779
SfaNI GCATC 3 cut(s) 473, 527, 721
SfcI CTRYAG 1 cut(s) 455
SinI GGWCC 2 cut(s) 298, 381
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse8387I CCTGCAGG 1 cut(s) 459
Sse9I AATT 1 cut(s) 539
SsiI CCGC 4 cut(s) 84, 216, 279, 755
StyI CCWWGG 2 cut(s) 165, 735
TaaI ACNGT 4 cut(s) 34, 289, 491, 640
TaqI TCGA 4 cut(s) 29, 306, 675, 702
TaqII GACCGA 1 cut(s) 164
TasI AATT 1 cut(s) 539
TfiI GAWTC 1 cut(s) 751
TscAI CASTG 1 cut(s) 507
TseI GCWGC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 298, 381
XapI RAATTY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.