FvH4_7g15520

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002430
Physical Location & Seq
Forward (+)
13420616 .. 13423029
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15520.t1

Sequence Viewer

Length: 774 bp
ATGGCGGAGCACGGTGAGGACGCCAAGGCGGAGCACGGTCTGATGGAGAAGATGACCGAGAAGATCCACGATCATCATGACTCGTCGAGTTCGTCGTCTTCGGATTCCGACACCGAGAAGCCGAAGCCGGCGGCGGAGGAGTCGCCGAAGGAGAAGGTGTTCCGTCTCTTCGGAAGGGAGCGCCCTGTTCATCATGTCTTGGGCGGTGGAAAGCCTGCTGATGTGTTCTTGTGGAGGAACAAGAAGATTTCTGCTGGGGTTCTTGGAGGAGCCACTGTCGTTTATGTTCTTTTTGAGTTGGTTGAATACCATTTGCTCACACTTATCTGCCACATTCTGATTGGTGCCCTAGCAGTGATGTTCCTGTGGTCCAATGCCCATACTATTTTCCACAAGACTGCACCACACATCCCAGAGGTTTCTCTTCCTGAGGAGCCGTTCCTGCAGGTTGCTTCTGAGCTGAGGCTTCAAATCAACTGGGCTTTTGCACTCCTTCATAATATTGCATCTGGAAGAGAGTTGAAGAAATTCCTGATTGTTATTGCTGGCTTGTGGGTTCTTTCTATTGTTGGGAGTTGGTGCAATTTCTTGACCTTGTTTTACATAACTTTTGTTCTCCTGCACACCGTGCCTGTTCTGTATGAGAAGTATGAACACAAGGTTGACCCACTAGCAGAGAAGGCGACAATTGAGATGAAAAAGCAGTATGGAGTTTTTGATGCCAAGGTTGTGACAAAGGTTCGTGAAACTGTGCTGAAAAACAAGAGGTCGTAG

Protein Analysis

258

Amino Acids

29.0

Weight (kDa)

7.28

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 73 - 228 9.2e-57 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 344
AciI CCGC 5 cut(s) 5, 29, 131, 134, 204
AclWI GGATC 1 cut(s) 58
AcsI RAATTY 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 628
AgsI TTSAA 3 cut(s) 305, 470, 523
AloI GAACNNNNNNTCC 2 cut(s) 143, 175
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw21I GWGCWC 2 cut(s) 12, 36
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 527
Asp700I GAANNNNTTC 2 cut(s) 158, 527
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 369
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 369
AxyI CCTNAGG 1 cut(s) 429
BaeGI GKGCMC 1 cut(s) 349
BanI GGYRCC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 2 cut(s) 12, 36
BbvCI CCTCAGC 1 cut(s) 461
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 2 cut(s) 350, 671
BfmI CTRYAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 3 cut(s) 271, 346, 435
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 2 cut(s) 515, 709
BmuI ACTGGG 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 24, 723
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 261, 291
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 482
Bse21I CCTNAGG 1 cut(s) 429
BseDI CCNNGG 2 cut(s) 24, 723
BseGI GGATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 420, 447, 452
BseNI ACTGG 1 cut(s) 482
BseRI GAGGAG 3 cut(s) 152, 282, 446
BseSI GKGCMC 1 cut(s) 349
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 2 cut(s) 384, 605
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 2 cut(s) 12, 36
BsiSI CCGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 12, 36, 349
Bsp143I GATC 2 cut(s) 63, 70
BspACI CCGC 5 cut(s) 5, 29, 131, 134, 204
BspCNI CTCAG 3 cut(s) 421, 448, 453
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 271, 346, 435
BspMAI CTGCAG 1 cut(s) 447
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 24, 723
BssMI GATC 2 cut(s) 63, 70
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 2 cut(s) 24, 723
Bst4CI ACNGT 5 cut(s) 14, 38, 277, 628, 751
Bst6I CTCTTC 3 cut(s) 173, 429, 508
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 628
BstC8I GCNNGC 3 cut(s) 129, 216, 547
BstDEI CTNAG 3 cut(s) 429, 456, 461
BstF5I GGATG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 2 cut(s) 66, 73
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 63, 70
BstMWI GCNNNNNNNGC 3 cut(s) 442, 628, 680
BstSFI CTRYAG 1 cut(s) 443
BstSLI GKGCMC 1 cut(s) 349
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 429
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 273, 360
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 129, 216, 547
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 369
CseI GACGC 1 cut(s) 29
CviAII CATG 2 cut(s) 77, 194
CviJI RGCY 9 cut(s) 121, 127, 214, 272, 436, 460, 466, 482, 549
CviKI_1 RGCY 9 cut(s) 121, 127, 214, 272, 436, 460, 466, 482, 549
DdeI CTNAG 3 cut(s) 429, 456, 461
DpnI GATC 2 cut(s) 65, 72
DpnII GATC 2 cut(s) 63, 70
DraIII CACNNNGTG 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 173, 429, 508
EarI CTCTTC 3 cut(s) 173, 429, 508
EciI GGCGGA 3 cut(s) 20, 44, 149
Eco130I CCWWGG 2 cut(s) 24, 723
Eco47I GGWCC 1 cut(s) 369
Eco81I CCTNAGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 24, 723
ErhI CCWWGG 2 cut(s) 24, 723
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 2 cut(s) 80, 197
FaiI YATR 9 cut(s) 78, 195, 285, 381, 498, 605, 642, 651, 708
FatI CATG 2 cut(s) 76, 193
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 2 cut(s) 350, 671
GlaI GCGC 1 cut(s) 182
GluI GCNGC 1 cut(s) 132
GsaI CCCAGC 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 1 cut(s) 128
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 80, 197
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 3 cut(s) 80, 104, 140
HpaII CCGG 1 cut(s) 128
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 6 cut(s) 42, 103, 109, 173, 339, 457
Hpy188III TCNNGA 6 cut(s) 77, 428, 510, 532, 589, 743
Hpy8I GTNNAC 1 cut(s) 664
Hpy99I CGWCG 2 cut(s) 88, 97
HpyAV CCTTC 5 cut(s) 142, 148, 168, 503, 673
HpyCH4III ACNGT 5 cut(s) 14, 38, 277, 628, 751
HpyCH4V TGCA 6 cut(s) 401, 445, 488, 506, 582, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 442, 628, 680
HpyF3I CTNAG 3 cut(s) 429, 456, 461
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 80, 197
HspAI GCGC 1 cut(s) 181
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 63, 70
LmnI GCTCC 5 cut(s) 7, 31, 178, 269, 433
LweI GCATC 2 cut(s) 515, 709
MaeI CTAG 2 cut(s) 350, 671
MaeIII GTNAC 1 cut(s) 730
MalI GATC 2 cut(s) 65, 72
MboI GATC 2 cut(s) 63, 70
MboII GAAGA 8 cut(s) 61, 73, 90, 160, 256, 416, 525, 535
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 3 cut(s) 12, 36, 349
MluCI AATT 3 cut(s) 527, 583, 687
MlyI GAGTC 2 cut(s) 74, 149
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 8 cut(s) 10, 130, 228, 260, 409, 424, 456, 759
MroNI GCCGGC 1 cut(s) 127
MroXI GAANNNNTTC 2 cut(s) 158, 527
MspI CCGG 1 cut(s) 128
MunI CAATTG 1 cut(s) 687
MwoI GCNNNNNNNGC 3 cut(s) 442, 628, 680
NaeI GCCGGC 1 cut(s) 129
NdeII GATC 2 cut(s) 63, 70
NgoMIV GCCGGC 1 cut(s) 127
NlaIII CATG 2 cut(s) 80, 197
NlaIV GGNNCC 3 cut(s) 271, 346, 435
NmuCI GTSAC 1 cut(s) 730
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 92
PdiI GCCGGC 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 158, 527
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 2 cut(s) 74, 148
PpsI GAGTC 2 cut(s) 74, 148
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 3 cut(s) 271, 346, 435
PspPI GGNCC 1 cut(s) 369
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 63
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 2 cut(s) 63, 70
Sau96I GGNCC 1 cut(s) 369
SbfI CCTGCAGG 1 cut(s) 447
SchI GAGTC 2 cut(s) 74, 149
SdaI CCTGCAGG 1 cut(s) 447
SduI GDGCHC 3 cut(s) 12, 36, 349
SetI ASST 9 cut(s) 159, 420, 450, 462, 596, 663, 729, 741, 770
SfaNI GCATC 2 cut(s) 515, 709
SfcI CTRYAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 369
Sse8387I CCTGCAGG 1 cut(s) 447
Sse9I AATT 3 cut(s) 527, 583, 687
SsiI CCGC 5 cut(s) 5, 29, 131, 134, 204
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 350, 671
StyI CCWWGG 2 cut(s) 24, 723
TaaI ACNGT 5 cut(s) 14, 38, 277, 628, 751
TaqI TCGA 1 cut(s) 86
TaqII GACCGA 1 cut(s) 71
TasI AATT 3 cut(s) 527, 583, 687
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 104
TscAI CASTG 2 cut(s) 280, 360
TseFI GTSAC 1 cut(s) 730
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 4 cut(s) 179, 485, 666, 710
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 280, 360
VpaK11BI GGWCC 1 cut(s) 369
XapI RAATTY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmnI GAANNNNTTC 2 cut(s) 158, 527
XspI CTAG 2 cut(s) 350, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.