AT4G01270

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001668
Physical Location & Seq
Forward (+)
532247 .. 534984
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01270.1

Sequence Viewer

Length: 1521 bp
ATGGCCGAAGAAAACGCGGCGGGGAATGCAATTTGTTCAATCTGCTACGAAGATCTTAAACCGGTGGTTGAGAATCTGCAATCAATCTCCGCTTGTGGCCATGTCTTTCACGAACTTTGTTTGCAGCAATGGTTTGAGTATTGCCCGAGTACGAACAAGCGAAATTGTCCGATTTGTAAGCAGAAGTGCTCTCTAAAGGATCCTTGTCGACTTTACTTTCAGTCTTCTGGGAATCAAACCGATTCGATTGCCTCGGATAAGGTTGTTGGAATCGAAGAAGATCCGGTTTTGTTGAGAGGAGAAGTGAAGCGGCTTGAAGGGAAGGTACAGAATCTTACTTCGGCTCTGGAGGCGAAGAAAAAGGAGAATGTTGAAGTCTCTGATAAGTTGCATCAATGCAATGAGCAATTGAAGGAGGATAAAGTGAAAAGGTGGGAGGCTCTTCAAGAAATATCAACGACTCAGCATTTGCTTAAGTTAAAATCTGAGGAATGTATCCAGCTTAACTCACAGTGTGTTAAGCTACAGGAGAGAACTGTGGCTCTTGCCAAGGAGCTCGCATCGCTTAAATTGGTTTCTGATTTAAGCTTGGAGGAAGATGATGTACTGAAGTTGGCCTTGTTGGGGAATAACGCGAAGACGAAAGATACAATAGACACACTAGTCAAGTCCTTGGTTATTCGGAATAGGAGTTATAAGGAATTGCTAGCCAAGTGCAATCAGCTGGGAAGAGGCGAAGCACGATCTTCTGAGAAACTTGAAAAAGCTTTGGAAAAGATAGAAAAACTAAAGAAACGAATGAGGGAACTTGAGTTGATAACTGAAGAGAGAGAGAACAGAGCTCTACGGGACATAAACGTTTCAAAGAAATGCAGTTACACAGAAGTTTCCGAGCCTGCAATCGAGAGCATGTCTTCTTTCAGAATGCTTTCATCAGACAACAAAGTGGAGAAAATCTCCACACCACCTGGTAAATTAGAAGAAAAGGATGGCTTTACCATTCAAGGATCATGCTTAAGGGGAAGAGAAGACTCCTTCGTTAGCAGAACAGACTCGGTTATAGATGTAGATGATGATTATGTTCCTGAAACCAACACTTCTGGCATTAGAGATTGGAATACTAATATTGAAGAGAAAGGTGATAATTCCATGGTGAAGGATATAAAGTTCAACATCAGAAAAGACCCGACATCATCAGTGTCACCTTACAGCAATGGTAGTGGAAACATTTGGCAGTCAAGTGGAACAAATAGAAACCTTGGTAGATGGAGCAAACATGGAGAGAGAAACGAAGCAACTCCATCACTAGGAGGTTCTGTTCCAAGAAAAGATGATCTCATCTCTATTGGACCTGATGGTAAAGGTGGTAGAATCAAAGTGCTGAGATCTAAACCCCAAATTTCTAAAACCAATGCAAGCTCAGGAAGTGGTAAGAGATTCAAGCTTGGAACTAAAACAAGTGGCTCGTCCTCTCAAGGTTGTCTTCAGATAGAACACTATTTCGGAAAAACTAATCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

506

Amino Acids

56.52

Weight (kDa)

8.44

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 12 - 60 3.8e-08 Ring finger domain
zf-RING_UBOX PF13445 12 - 57 3.8e-07 RING-type zinc-finger
zf-C3HC4 PF00097 12 - 59 5.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AasI GACNNNNNNGTC 1 cut(s) 662
AccI GTMKAC 1 cut(s) 208
AccII CGCG 2 cut(s) 17, 635
AciI CCGC 4 cut(s) 17, 20, 90, 310
AclI AACGTT 1 cut(s) 858
AclWI GGATC 4 cut(s) 194, 207, 275, 1015
AcoI YGGCCR 2 cut(s) 3, 97
AcsI RAATTY 1 cut(s) 1398
AcuI CTGAAG 3 cut(s) 629, 843, 1469
AdeI CACNNNGTG 1 cut(s) 515
AfaI GTAC 3 cut(s) 151, 327, 606
AfiI CCNNNNNNNGG 2 cut(s) 624, 1307
AflII CTTAAG 2 cut(s) 473, 1015
AgeI ACCGGT 1 cut(s) 61
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 1 cut(s) 967
AloI GAACNNNNNNTCC 4 cut(s) 1151, 1183, 1302, 1334
AluBI AGCT 9 cut(s) 502, 523, 556, 588, 724, 767, 842, 1419, 1444
AluI AGCT 9 cut(s) 502, 523, 556, 588, 724, 767, 842, 1419, 1444
Alw21I GWGCWC 3 cut(s) 191, 558, 844
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 4 cut(s) 194, 207, 275, 1015
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 3 cut(s) 3, 97, 615
ApeKI GCWGC 1 cut(s) 124
ApoI RAATTY 1 cut(s) 1398
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
AsiGI ACCGGT 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 928
AspS9I GGNCC 1 cut(s) 1349
AsuHPI GGTGA 3 cut(s) 1151, 1165, 1194
AsuNHI GCTAGC 1 cut(s) 706
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 1349
BalI TGGCCA 1 cut(s) 99
BamHI GGATCC 1 cut(s) 199
BanII GRGCYC 2 cut(s) 558, 844
BarI GAAGNNNNNNTAC 4 cut(s) 309, 341, 588, 620
BbsI GAAGAC 5 cut(s) 216, 644, 906, 1035, 1475
Bbv12I GWGCWC 3 cut(s) 191, 558, 844
BbvI GCAGC 1 cut(s) 136
BccI CCATC 4 cut(s) 983, 1260, 1309, 1349
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 969
BciVI GTATCC 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 382
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 3 cut(s) 662, 707, 1307
BfmI CTRYAG 1 cut(s) 524
BfrI CTTAAG 2 cut(s) 473, 1015
BfuI GTATCC 1 cut(s) 506
BglII AGATCT 2 cut(s) 52, 1385
BisI GCNGC 3 cut(s) 18, 125, 311
BlsI GCNGC 3 cut(s) 19, 126, 312
Bme1390I CCNGG 1 cut(s) 969
Bme18I GGWCC 1 cut(s) 1349
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 1349
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 969
BmsI GCATC 2 cut(s) 400, 569
BmtI GCTAGC 1 cut(s) 710
BpiI GAAGAC 5 cut(s) 216, 644, 906, 1035, 1475
BplI GAGNNNNNCTC 2 cut(s) 941, 973
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 1420
BpuEI CTTGAG 2 cut(s) 830, 1458
BsaJI CCNNGG 5 cut(s) 252, 549, 672, 1149, 1258
BsaWI WCCGGW 2 cut(s) 61, 283
BsaXI ACNNNNNCTCC 2 cut(s) 1302, 1332
Bsc4I CCNNNNNNNGG 2 cut(s) 624, 1307
Bse118I RCCGGY 1 cut(s) 61
Bse3DI GCAATG 3 cut(s) 134, 406, 1219
BseBI CCWGG 1 cut(s) 969
BseDI CCNNGG 5 cut(s) 252, 549, 672, 1149, 1258
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 2 cut(s) 624, 1307
BseMI GCAATG 3 cut(s) 134, 406, 1219
BseMII CTCAG 5 cut(s) 476, 477, 741, 1373, 1434
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 724
Bsh1236I CGCG 2 cut(s) 17, 635
BshFI GGCC 3 cut(s) 5, 99, 617
BshTI ACCGGT 1 cut(s) 61
BsiHKAI GWGCWC 3 cut(s) 191, 558, 844
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 2 cut(s) 62, 284
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 2 cut(s) 624, 1307
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 863
BsmI GAATGC 2 cut(s) 31, 930
BsnI GGCC 3 cut(s) 5, 99, 617
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 3 cut(s) 191, 558, 844
Bsp143I GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 4 cut(s) 17, 20, 90, 310
BspANI GGCC 3 cut(s) 5, 99, 617
BspCNI CTCAG 5 cut(s) 475, 478, 742, 1374, 1433
BspFNI CGCG 2 cut(s) 17, 635
BspLI GGNNCC 1 cut(s) 201
BspOI GCTAGC 1 cut(s) 710
BspPI GGATC 4 cut(s) 194, 207, 275, 1015
BspQI GCTCTTC 1 cut(s) 447
BspTI CTTAAG 2 cut(s) 473, 1015
BsrDI GCAATG 3 cut(s) 134, 406, 1219
BsrFI RCCGGY 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 5 cut(s) 252, 549, 672, 1149, 1258
BssMI GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
BssT1I CCWWGG 4 cut(s) 549, 672, 1149, 1258
Bst2UI CCWGG 1 cut(s) 969
Bst4CI ACNGT 2 cut(s) 513, 538
Bst6I CTCTTC 5 cut(s) 447, 724, 819, 1018, 1125
BstAFI CTTAAG 2 cut(s) 473, 1015
BstC8I GCNNGC 4 cut(s) 558, 708, 897, 1417
BstDEI CTNAG 5 cut(s) 462, 486, 750, 1382, 1420
BstDSI CCRYGG 1 cut(s) 1149
BstF5I GGATG 1 cut(s) 994
BstFNI CGCG 2 cut(s) 17, 635
BstKTI GATC 7 cut(s) 55, 202, 283, 746, 1010, 1336, 1388
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
BstMWI GCNNNNNNNGC 3 cut(s) 26, 350, 562
BstNI CCWGG 1 cut(s) 969
BstNSI RCATGY 1 cut(s) 913
BstSCI CCNGG 1 cut(s) 967
BstSFI CTRYAG 1 cut(s) 524
BstUI CGCG 2 cut(s) 17, 635
BstV1I GCAGC 1 cut(s) 136
BstV2I GAAGAC 5 cut(s) 216, 644, 906, 1035, 1475
BstX2I RGATCY 4 cut(s) 52, 199, 280, 1385
BstYI RGATCY 4 cut(s) 52, 199, 280, 1385
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 3 cut(s) 5, 99, 617
BtgI CCRYGG 1 cut(s) 1149
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 1 cut(s) 994
BtsIMutI CAGTG 2 cut(s) 518, 1203
Cac8I GCNNGC 4 cut(s) 558, 708, 897, 1417
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 1349
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 150, 326, 605
CspAI ACCGGT 1 cut(s) 61
CspCI CAANNNNNGTGG 2 cut(s) 1201, 1236
CviAII CATG 5 cut(s) 101, 910, 1011, 1150, 1277
CviQI GTAC 3 cut(s) 150, 326, 605
DdeI CTNAG 5 cut(s) 462, 486, 750, 1382, 1420
DpnI GATC 7 cut(s) 54, 201, 282, 745, 1009, 1335, 1387
DpnII GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
DraIII CACNNNGTG 1 cut(s) 515
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 2 cut(s) 3, 97
Eam1104I CTCTTC 5 cut(s) 447, 724, 819, 1018, 1125
EarI CTCTTC 5 cut(s) 447, 724, 819, 1018, 1125
Ecl136II GAGCTC 2 cut(s) 556, 842
Eco130I CCWWGG 4 cut(s) 549, 672, 1149, 1258
Eco24I GRGCYC 2 cut(s) 558, 844
Eco47I GGWCC 1 cut(s) 1349
Eco53kI GAGCTC 2 cut(s) 556, 842
Eco57I CTGAAG 3 cut(s) 629, 843, 1469
Eco88I CYCGRG 1 cut(s) 145
EcoICRI GAGCTC 2 cut(s) 556, 842
EcoRII CCWGG 1 cut(s) 967
EcoT14I CCWWGG 4 cut(s) 549, 672, 1149, 1258
EcoT38I GRGCYC 2 cut(s) 558, 844
ErhI CCWWGG 4 cut(s) 549, 672, 1149, 1258
FaeI CATG 5 cut(s) 104, 913, 1014, 1153, 1280
FalI AAGNNNNNCTT 6 cut(s) 602, 634, 977, 1009, 1467, 1499
FaqI GGGAC 1 cut(s) 863
FatI CATG 5 cut(s) 100, 909, 1010, 1149, 1276
FauI CCCGC 1 cut(s) 13
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 18, 125, 311
FokI GGATG 1 cut(s) 1001
FriOI GRGCYC 2 cut(s) 558, 844
Fsp4HI GCNGC 3 cut(s) 18, 125, 311
FspBI CTAG 3 cut(s) 662, 707, 1307
GluI GCNGC 3 cut(s) 18, 125, 311
GsaI CCCAGC 1 cut(s) 728
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 3 cut(s) 5, 99, 617
HapII CCGG 2 cut(s) 62, 284
Hin1II CATG 5 cut(s) 104, 913, 1014, 1153, 1280
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HindIII AAGCTT 3 cut(s) 586, 765, 1442
HpaII CCGG 2 cut(s) 62, 284
HphI GGTGA 3 cut(s) 1151, 1165, 1194
Hpy166II GTNNAC 1 cut(s) 209
Hpy188III TCNNGA 6 cut(s) 110, 347, 446, 904, 1085, 1422
Hpy8I GTNNAC 1 cut(s) 209
HpyAV CCTTC 5 cut(s) 311, 316, 406, 1045, 1150
HpyCH4III ACNGT 2 cut(s) 513, 538
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 9 cut(s) 29, 79, 124, 391, 399, 717, 873, 899, 1415
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 350, 562
HpyF3I CTNAG 5 cut(s) 462, 486, 750, 1382, 1420
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 5 cut(s) 104, 913, 1014, 1153, 1280
Kzo9I GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 2 cut(s) 553, 1269
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 2 cut(s) 400, 569
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 3 cut(s) 662, 707, 1307
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 2 cut(s) 875, 1200
MalI GATC 7 cut(s) 54, 201, 282, 745, 1009, 1335, 1387
MboI GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
MfeI CAATTG 1 cut(s) 407
MflI RGATCY 4 cut(s) 52, 199, 280, 1385
MhlI GDGCHC 3 cut(s) 191, 558, 844
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 8 cut(s) 30, 163, 407, 569, 701, 974, 1144, 1398
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 3 cut(s) 454, 1025, 1046
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 99
MroXI GAANNNNTTC 1 cut(s) 928
MscI TGGCCA 1 cut(s) 99
MseI TTAA 8 cut(s) 57, 474, 479, 504, 519, 567, 584, 1016
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 724
MspCI CTTAAG 2 cut(s) 473, 1015
MspI CCGG 2 cut(s) 62, 284
MspR9I CCNGG 1 cut(s) 969
MunI CAATTG 1 cut(s) 407
Mva1269I GAATGC 2 cut(s) 31, 930
MvaI CCWGG 1 cut(s) 969
MvnI CGCG 2 cut(s) 17, 635
MwoI GCNNNNNNNGC 3 cut(s) 26, 350, 562
NcoI CCATGG 1 cut(s) 1149
NdeII GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
NheI GCTAGC 1 cut(s) 706
NlaIII CATG 5 cut(s) 104, 913, 1014, 1153, 1280
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 1200
NspI RCATGY 1 cut(s) 913
PciSI GCTCTTC 1 cut(s) 447
PctI GAATGC 2 cut(s) 31, 930
PdmI GAANNNNTTC 1 cut(s) 928
PfeI GAWTC 7 cut(s) 73, 232, 242, 270, 331, 1371, 1437
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 3 cut(s) 19, 126, 312
PleI GAGTC 3 cut(s) 454, 1025, 1046
PpsI GAGTC 3 cut(s) 454, 1025, 1046
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 2 cut(s) 558, 844
Psp1406I AACGTT 1 cut(s) 858
Psp6I CCWGG 1 cut(s) 967
PspFI CCCAGC 1 cut(s) 724
PspGI CCWGG 1 cut(s) 967
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 1349
PsuI RGATCY 4 cut(s) 52, 199, 280, 1385
PvuII CAGCTG 1 cut(s) 724
RsaI GTAC 3 cut(s) 151, 327, 606
RsaNI GTAC 3 cut(s) 150, 326, 605
SacI GAGCTC 2 cut(s) 558, 844
SalI GTCGAC 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 8 cut(s) 57, 474, 479, 504, 519, 567, 584, 1016
SatI GCNGC 3 cut(s) 18, 125, 311
Sau3AI GATC 7 cut(s) 52, 199, 280, 743, 1007, 1333, 1385
Sau96I GGNCC 1 cut(s) 1349
SchI GAGTC 3 cut(s) 454, 1025, 1046
ScrFI CCNGG 1 cut(s) 969
SduI GDGCHC 3 cut(s) 191, 558, 844
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 2 cut(s) 400, 569
SfcI CTRYAG 1 cut(s) 524
SinI GGWCC 1 cut(s) 1349
SmlI CTYRAG 4 cut(s) 473, 809, 1015, 1473
SmoI CTYRAG 4 cut(s) 473, 809, 1015, 1473
SpeI ACTAGT 1 cut(s) 661
Sse9I AATT 8 cut(s) 30, 163, 407, 569, 701, 974, 1144, 1398
SsiI CCGC 4 cut(s) 17, 20, 90, 310
SspI AATATT 1 cut(s) 1126
SspMI CTAG 3 cut(s) 662, 707, 1307
SstI GAGCTC 2 cut(s) 558, 844
StyD4I CCNGG 1 cut(s) 967
StyI CCWWGG 4 cut(s) 549, 672, 1149, 1258
TaaI ACNGT 2 cut(s) 513, 538
TaiI ACGT 1 cut(s) 861
TaqI TCGA 4 cut(s) 208, 245, 273, 903
TasI AATT 8 cut(s) 30, 163, 407, 569, 701, 974, 1144, 1398
TatI WGTACW 1 cut(s) 604
TauI GCSGC 2 cut(s) 20, 313
TfiI GAWTC 7 cut(s) 73, 232, 242, 270, 331, 1371, 1437
Tru1I TTAA 8 cut(s) 57, 474, 479, 504, 519, 567, 584, 1016
Tru9I TTAA 8 cut(s) 57, 474, 479, 504, 519, 567, 584, 1016
TscAI CASTG 2 cut(s) 518, 1203
TseFI GTSAC 1 cut(s) 1200
TseI GCWGC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 1200
TspDTI ATGAA 1 cut(s) 921
TspRI CASTG 2 cut(s) 518, 1203
Vha464I CTTAAG 2 cut(s) 473, 1015
VpaK11BI GGWCC 1 cut(s) 1349
XapI RAATTY 1 cut(s) 1398
XceI RCATGY 1 cut(s) 913
XmiI GTMKAC 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 928
XspI CTAG 3 cut(s) 662, 707, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.