Rh2AG228300

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001668
Physical Location & Seq
Forward (+)
24246134 .. 24251834
5701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG228300.1

Sequence Viewer

Length: 1476 bp
ATGGTCAATTGCGACCACCACCACGACTCTAGCTTCAGCAAAGCCATCTGCTCAATCTGCCACAAAGACCTCGAGCCCTTCCTCGAGGACCTCCAAGCCATCTTCATCTGCGGCCACGTCTTCCACGAGTTCTGCTTGCAAGAGTCCTTCGGCTTCTTTGAGAGCGTCAAGAGGTACAGCTGTCCGGTGTGCAAACAGCGATGCAAGCCGAACGACGTCGCTCGGCTCTATTTCGAGTCGGTGGGCATCTCGCCGGAGCCGCCGAGTTTGACTCGGTCGCCGATTGGGGAAGATTCCGAGGCATTGCATCGTGAGGTCAAAGAGCTTAAAGAGGAGCTTTATTTATGCAAGGAGCAAGCGAAGAAAGAAGTGGCTAATTTAAAGATTGAAGCTCTGAAACAACAAGCATCTATACGACAGATGCTTCATATGAAAACTGAGGCACTTGATAAATTGACTGTGGAGTGCTTGCGGCTGCAAGAAAGAAATAGGGCACTGACCAAAGAACTTGCAGCTTTCAAATTGGCATCTGATCTAGATCTCGATGAAAAGGAGATTTTGAACCTTGCCGCACTTGGTAATGGGGGAAACAATGAAAACACAATAGATGTTCTTAGAAAATCTTTGATTATGAGTAATAGGAATCGCAAAGAACTGATTGCCCAGCATATTCTTCTTGAACGAGGAGAGGCTCTTCACCGTAAAAAACTAGAGAAGGCTAAAGGAAAGATAAATAGATTAAATACAAAGGTACAGGAATTGGCAGACCAGTGCAATCTTCTTGAACGAGGTGAGGCTCGTTGTAGTAGAAAGCTAGAGAAGGCTAAAGGAAAGATAAACAAATTGAAGACAAAGGTACAGAAACTGAAGGACCAATGCAATCTTCTTGAACAAGGTGAGGCTCGTTACAGTAGAAAGATAGAGAAGGCTAAAGGGAAGATAAATACATTGAAGGCAAGGGTACAAGAATTTGATGGTGCTGTTGAAGTAAGGAAAGAAACTTCAGTCCTTGGACCAAGCGATCTGTTTGAGAGTATGGTTCCTGTACAATCCATGCTTAACATTACAAAGGAATCTCCAATCACCCAGCCACTTGCCAGACCAGATGCTGTGATTCTTGATGATCTCAACCAAGTTCAACCCATGCTTAATAGTAGCAAGGAATCTCCGATTACTCAACCACTTCCCAGACCAGTGAGTGCATATTTCTCTGGTGGCTTGGTTGGTCCTGATGGAACGAAAAGGTTTTTAGGTAAATGGTGCAAGCGGAGCTTGAACAATCAATTGGTAACAAACAAATGTTCAAGTACAGATGCTGGCTGTACGATTGTTGTAGTATCTGATGAAGAGGATATTACCGAAAATACACCGCAGACAAAGAGGTGCAAGTATGAAGATAAGTCAAAAATTGTACAGTCTCAAGGGTGCTTGCAGTACAACACTTCTTTGGAAGGGTCGGTAACTACGAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

491

Amino Acids

55.35

Weight (kDa)

8.72

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
AbsI CCTCGAGG 1 cut(s) 83
AciI CCGC 6 cut(s) 111, 260, 472, 570, 1267, 1370
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 968
AcuI CTGAAG 3 cut(s) 19, 887, 987
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 9 cut(s) 176, 753, 858, 963, 1047, 1309, 1324, 1413, 1436
AjiI CACGTC 1 cut(s) 118
AjuI GAANNNNNNNTTGG 4 cut(s) 867, 899, 1268, 1300
AluBI AGCT 8 cut(s) 33, 180, 325, 337, 392, 515, 814, 1272
AluI AGCT 8 cut(s) 33, 180, 325, 337, 392, 515, 814, 1272
Alw26I GTCTC 1 cut(s) 1422
AlwNI CAGNNNCTG 3 cut(s) 865, 1109, 1316
Ama87I CYCGRG 2 cut(s) 71, 83
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 475, 512
ApoI RAATTY 1 cut(s) 968
AspS9I GGNCC 4 cut(s) 88, 871, 1013, 1226
AsuHPI GGTGA 4 cut(s) 689, 803, 908, 1075
AvaI CYCGRG 2 cut(s) 71, 83
AvaII GGWCC 4 cut(s) 88, 871, 1013, 1226
BaeGI GKGCMC 1 cut(s) 496
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BanII GRGCYC 1 cut(s) 78
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 2 cut(s) 112, 854
BbvI GCAGC 2 cut(s) 462, 524
BccI CCATC 4 cut(s) 53, 107, 968, 1226
BcoDI GTCTC 1 cut(s) 1422
BfaI CTAG 4 cut(s) 30, 536, 710, 815
BglII AGATCT 1 cut(s) 538
BisI GCNGC 6 cut(s) 112, 260, 473, 476, 513, 570
BlsI GCNGC 6 cut(s) 113, 261, 474, 477, 514, 571
Bme18I GGWCC 4 cut(s) 88, 871, 1013, 1226
BmeT110I CYCGRG 2 cut(s) 71, 83
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 4 cut(s) 88, 871, 1013, 1226
BmiI GGNNCC 2 cut(s) 258, 1041
BmsI GCATC 8 cut(s) 191, 255, 316, 411, 416, 536, 1096, 1303
BpiI GAAGAC 2 cut(s) 112, 854
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BpuEI CTTGAG 1 cut(s) 1404
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 297, 1009
BsaWI WCCGGW 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 769, 1193
Bse3DI GCAATG 1 cut(s) 302
Bse8I GATNNNNATC 1 cut(s) 537
BseDI CCNNGG 2 cut(s) 297, 1009
BseJI GATNNNNATC 1 cut(s) 537
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 769, 1193
BseRI GAGGAG 2 cut(s) 347, 699
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 2 cut(s) 462, 524
BseYI CCCAGC 2 cut(s) 663, 1086
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 278
BsiHKCI CYCGRG 2 cut(s) 71, 83
BsiSI CCGG 2 cut(s) 185, 254
BsmAI GTCTC 1 cut(s) 1422
BsnI GGCC 1 cut(s) 114
BsoBI CYCGRG 2 cut(s) 71, 83
Bsp1286I GDGCHC 2 cut(s) 78, 496
Bsp1407I TGTACA 2 cut(s) 1045, 1411
Bsp143I GATC 4 cut(s) 532, 538, 1021, 1123
BspACI CCGC 6 cut(s) 111, 260, 472, 570, 1267, 1370
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 258, 1041
BspQI GCTCTTC 1 cut(s) 699
BsrDI GCAATG 1 cut(s) 302
BsrGI TGTACA 2 cut(s) 1045, 1411
BsrI ACTGG 2 cut(s) 769, 1193
BssECI CCNNGG 2 cut(s) 297, 1009
BssMI GATC 4 cut(s) 532, 538, 1021, 1123
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 1009
Bst2BI CACGAG 1 cut(s) 125
Bst4CI ACNGT 4 cut(s) 460, 701, 911, 1416
Bst6I CTCTTC 2 cut(s) 699, 1341
BstACI GRCGYC 1 cut(s) 216
BstAUI TGTACA 2 cut(s) 1045, 1411
BstC8I GCNNGC 7 cut(s) 137, 206, 357, 470, 1265, 1318, 1430
BstDEI CTNAG 2 cut(s) 438, 614
BstKTI GATC 4 cut(s) 535, 541, 1024, 1126
BstMAI GTCTC 1 cut(s) 1422
BstMBI GATC 4 cut(s) 532, 538, 1021, 1123
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1269
BstSLI GKGCMC 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 462, 524
BstV2I GAAGAC 2 cut(s) 112, 854
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 214
BtrI CACGTC 1 cut(s) 118
BtsIMutI CAGTG 3 cut(s) 494, 776, 1200
Cac8I GCNNGC 7 cut(s) 137, 206, 357, 470, 1265, 1318, 1430
CaiI CAGNNNCTG 3 cut(s) 865, 1109, 1316
Cfr13I GGNCC 4 cut(s) 88, 871, 1013, 1226
CseI GACGC 1 cut(s) 154
Csp6I GTAC 9 cut(s) 175, 752, 857, 962, 1046, 1308, 1323, 1412, 1435
CviAII CATG 2 cut(s) 1054, 1144
CviQI GTAC 9 cut(s) 175, 752, 857, 962, 1046, 1308, 1323, 1412, 1435
DdeI CTNAG 2 cut(s) 438, 614
DpnI GATC 4 cut(s) 534, 540, 1023, 1125
DpnII GATC 4 cut(s) 532, 538, 1021, 1123
DraI TTTAAA 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 699, 1341
EarI CTCTTC 2 cut(s) 699, 1341
Eco130I CCWWGG 1 cut(s) 1009
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 4 cut(s) 88, 871, 1013, 1226
Eco57I CTGAAG 3 cut(s) 19, 887, 987
Eco88I CYCGRG 2 cut(s) 71, 83
EcoO109I RGGNCCY 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 1009
FaeI CATG 2 cut(s) 1057, 1147
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 1256, 1288
FatI CATG 2 cut(s) 1053, 1143
FauNDI CATATG 1 cut(s) 429
Fnu4HI GCNGC 6 cut(s) 112, 260, 473, 476, 513, 570
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 6 cut(s) 112, 260, 473, 476, 513, 570
FspBI CTAG 4 cut(s) 30, 536, 710, 815
GluI GCNGC 6 cut(s) 112, 260, 473, 476, 513, 570
GsaI CCCAGC 2 cut(s) 667, 1090
HaeIII GGCC 1 cut(s) 114
HapII CCGG 2 cut(s) 185, 254
HgaI GACGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 1057, 1147
HinfI GANTC 9 cut(s) 26, 143, 236, 271, 293, 643, 1073, 1114, 1163
HpaII CCGG 2 cut(s) 185, 254
HphI GGTGA 4 cut(s) 689, 803, 908, 1075
Hpy188I TCNGA 5 cut(s) 298, 396, 532, 1170, 1342
Hpy188III TCNNGA 9 cut(s) 169, 311, 536, 542, 677, 782, 887, 1118, 1229
Hpy99I CGWCG 2 cut(s) 218, 221
HpyAV CCTTC 8 cut(s) 88, 157, 709, 814, 862, 919, 946, 1445
HpyCH4III ACNGT 4 cut(s) 460, 701, 911, 1416
HpyCH4IV ACGT 2 cut(s) 117, 216
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1269
HpyF3I CTNAG 2 cut(s) 438, 614
HpySE526I ACGT 2 cut(s) 117, 216
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 1057, 1147
Kzo9I GATC 4 cut(s) 532, 538, 1021, 1123
LguI GCTCTTC 1 cut(s) 699
LmnI GCTCC 4 cut(s) 256, 334, 352, 1269
Lsp1109I GCAGC 2 cut(s) 462, 524
LweI GCATC 8 cut(s) 191, 255, 316, 411, 416, 536, 1096, 1303
MaeI CTAG 4 cut(s) 30, 536, 710, 815
MaeII ACGT 2 cut(s) 117, 216
MaeIII GTNAC 3 cut(s) 905, 1288, 1459
MalI GATC 4 cut(s) 534, 540, 1023, 1125
MboI GATC 4 cut(s) 532, 538, 1021, 1123
MfeI CAATTG 2 cut(s) 7, 1283
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 78, 496
MluCI AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1283, 1407
MlyI GAGTC 4 cut(s) 20, 152, 245, 265
MseI TTAA 5 cut(s) 327, 380, 740, 1059, 1149
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 2 cut(s) 185, 254
MunI CAATTG 2 cut(s) 7, 1283
MwoI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1269
NdeI CATATG 1 cut(s) 429
NdeII GATC 4 cut(s) 532, 538, 1021, 1123
NlaIII CATG 2 cut(s) 1057, 1147
NlaIV GGNNCC 2 cut(s) 258, 1041
NmeAIII GCCGAG 2 cut(s) 202, 288
PaeR7I CTCGAG 2 cut(s) 71, 83
PciSI GCTCTTC 1 cut(s) 699
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 1463
PfeI GAWTC 5 cut(s) 293, 643, 1073, 1114, 1163
PflFI GACNNNGTC 1 cut(s) 274
PkrI GCNGC 6 cut(s) 113, 261, 474, 477, 514, 571
PleI GAGTC 4 cut(s) 20, 151, 244, 265
PpsI GAGTC 4 cut(s) 20, 151, 244, 265
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
PspFI CCCAGC 2 cut(s) 663, 1086
PspN4I GGNNCC 2 cut(s) 258, 1041
PspPI GGNCC 4 cut(s) 88, 871, 1013, 1226
PspPPI RGGWCCY 1 cut(s) 88
PspXI VCTCGAGB 2 cut(s) 71, 83
PstNI CAGNNNCTG 3 cut(s) 865, 1109, 1316
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 180
RsaI GTAC 9 cut(s) 176, 753, 858, 963, 1047, 1309, 1324, 1413, 1436
RsaNI GTAC 9 cut(s) 175, 752, 857, 962, 1046, 1308, 1323, 1412, 1435
SapI GCTCTTC 1 cut(s) 699
SaqAI TTAA 5 cut(s) 327, 380, 740, 1059, 1149
SatI GCNGC 6 cut(s) 112, 260, 473, 476, 513, 570
Sau3AI GATC 4 cut(s) 532, 538, 1021, 1123
Sau96I GGNCC 4 cut(s) 88, 871, 1013, 1226
SchI GAGTC 4 cut(s) 20, 152, 245, 265
SduI GDGCHC 2 cut(s) 78, 496
SfaNI GCATC 8 cut(s) 191, 255, 316, 411, 416, 536, 1096, 1303
Sfr274I CTCGAG 2 cut(s) 71, 83
SinI GGWCC 4 cut(s) 88, 871, 1013, 1226
SlaI CTCGAG 2 cut(s) 71, 83
SmlI CTYRAG 3 cut(s) 71, 83, 1419
SmoI CTYRAG 3 cut(s) 71, 83, 1419
Sse9I AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1283, 1407
SsiI CCGC 6 cut(s) 111, 260, 472, 570, 1267, 1370
SspMI CTAG 4 cut(s) 30, 536, 710, 815
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 4 cut(s) 460, 701, 911, 1416
TaiI ACGT 2 cut(s) 120, 219
TaqI TCGA 4 cut(s) 72, 84, 234, 543
TaqII GACCGA 1 cut(s) 264
TasI AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1283, 1407
TatI WGTACW 4 cut(s) 1045, 1307, 1411, 1434
TauI GCSGC 4 cut(s) 114, 262, 475, 572
TfiI GAWTC 5 cut(s) 293, 643, 1073, 1114, 1163
Tru1I TTAA 5 cut(s) 327, 380, 740, 1059, 1149
Tru9I TTAA 5 cut(s) 327, 380, 740, 1059, 1149
TscAI CASTG 3 cut(s) 501, 776, 1200
TseI GCWGC 2 cut(s) 475, 512
TspDTI ATGAA 7 cut(s) 94, 416, 446, 561, 609, 1359, 1407
TspRI CASTG 3 cut(s) 501, 776, 1200
Tth111I GACNNNGTC 1 cut(s) 274
VpaK11BI GGWCC 4 cut(s) 88, 871, 1013, 1226
XapI RAATTY 1 cut(s) 968
XbaI TCTAGA 1 cut(s) 535
XhoI CTCGAG 2 cut(s) 71, 83
XspI CTAG 4 cut(s) 30, 536, 710, 815
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.