RLG00000017916

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001668
Physical Location & Seq
Forward (+)
22935253 .. 22938731
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017916

Sequence Viewer

Length: 1557 bp
ATGGTCAATTGCGACCACCACCACGACTCTAGCTTCAGCAAAGCCATCTGCTCAATCTGCCACAAAGACCTCGAGCCCTTCCTCGAGGACCTCCAAGCCATCTTCATCTGCGGCCACGTCTTCCACGAGTTCTGCTTGCAAGAGTCCTTCGGCTTCTTTGAGAGCGTCAAGAGGTACAGCTGTCCGGTGTGCAAACAGCGATGCAAGCCGAACGACGTCGCTCGGCTCTATTTCGAGTCGGTGGGCATCTCGCCGGAGCCGCCGAGTTTGACTCGCTCACCGATTTGGGAAGATTCCGAGGCATTGCGTCGTGAGGTCAAAGAGCTTAAAGAGGAGCTTTATTTATGCAAGGAGCAAGCGAAGAAAGAAGTGGCTAATTTAAAGAATGAAGCTCTGAAACAACAAGCATCTATACGACAGACGCTTCATATGAAAACTGAGGCGCTTGATAAATTGACTGTGGAGTGCTTGCGACTGCAAGAAAGAAATAGGGCACTGACCAAAGAGCTTGCAGCTTTCAAATTGGCATCTGATCTAGATCTCGATGAAAAGGAGATTTTGAAGCTTGCCGCACTTGGTAATGGGGGAAACAATGAAAACACAATAGATGTTCTTAGAAAATCTTTGATTATGAGTAATAGGAATCGCAAAGAACTGATTGCCCAGCATAATCTTCTTGAACGAGGAGAGGCTCTTCACCATAAAAAACTAGAGAAGGCTAAAGGAAAGATAAATAGATTAAATACAAAGGTACAGGAATTGACAGACCAGTGCAATCTTTTTGAACGAGGTGAGGCTCGTTGTAGTAGAAAGCTAGAGAAGGCTAAAGGAAAGATAAACAAATTGAAGACAAAGGTACAGAAACTGAAGGACCAATGCAATCTTCTCGAACAAGGTGAGGCTCGTTACAGTAGAAAGATAGAGAAGGCTAAAGGGAAGATAAATACATTGAAGGCAAGGGTACAAGAATTTGATGGTGCTGTTGAAGTAAGGAAAGAAACTTCAGTCCTTGGACCAAGCGATCTGTTTGAGAGTATGGTTCCTGTGAGTGGTACCTCTAATGATACTGCCAAAGCTCCTGCTGATGATATTGCAGATGTTCTGATCCTTGACAATGTTAAACAAGTCCAATCCATGCTTAACATTACAAAGGAATCTCCAATCACCCAGCCACTTGCCAGACCAGATGCTGTGATTCTTGATGATCTCAACCAAGTTCAACCCATGCTTAATAGTAGCAAGGAATCTCCGATTACACAACCACTTCCCAGACCGGTGAGTGCATATTTTTCTGGTGGCTTGGTTGGTCCTGATGGAACAAAAAGGTTTTTAGGTAAATGGTGCAAGCGGAGCTTGAACAATCAATTGGTAACAAACAAATATTCAAGTACAGATGCTGGCTGTTCGATTGTTGTAGTATCTGATGAAGAGGATATTACCGAAAATACACCGCAGACAAAGAGGTGCAAGTATGAAGATAGGTCAAAAATTGTACAGTCCCAAGGGTGCTTGCAGTACAACACTTCTTTGGAAGGGTCGGTAACTACGAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

519

Amino Acids

58.36

Weight (kDa)

8.59

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
AbsI CCTCGAGG 1 cut(s) 83
Acc65I GGTACC 1 cut(s) 1052
AccB1I GGYRCC 1 cut(s) 1052
AciI CCGC 5 cut(s) 111, 260, 570, 1348, 1451
AclWI GGATC 1 cut(s) 1099
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 968
AcuI CTGAAG 3 cut(s) 19, 887, 987
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 8 cut(s) 176, 753, 858, 963, 1054, 1390, 1494, 1517
AfiI CCNNNNNNNGG 1 cut(s) 1049
AgeI ACCGGT 1 cut(s) 1273
AjiI CACGTC 1 cut(s) 118
AjuI GAANNNNNNNTTGG 4 cut(s) 867, 899, 1349, 1381
AlwI GGATC 1 cut(s) 1099
AlwNI CAGNNNCTG 3 cut(s) 865, 1190, 1397
Ama87I CYCGRG 2 cut(s) 71, 83
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 1 cut(s) 968
AsiGI ACCGGT 1 cut(s) 1273
Asp718I GGTACC 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 4 cut(s) 88, 871, 1013, 1307
AsuHPI GGTGA 6 cut(s) 270, 689, 803, 908, 1156, 1288
AvaI CYCGRG 2 cut(s) 71, 83
AvaII GGWCC 4 cut(s) 88, 871, 1013, 1307
BaeGI GKGCMC 1 cut(s) 496
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BanI GGYRCC 1 cut(s) 1052
BanII GRGCYC 1 cut(s) 78
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 2 cut(s) 112, 854
BbvI GCAGC 1 cut(s) 524
BccI CCATC 4 cut(s) 53, 107, 968, 1307
BcgI CGANNNNNNTGC 2 cut(s) 868, 902
BfaI CTAG 4 cut(s) 30, 536, 710, 815
BfoI RGCGCY 1 cut(s) 446
BglII AGATCT 1 cut(s) 538
BisI GCNGC 4 cut(s) 112, 260, 513, 570
BlsI GCNGC 4 cut(s) 113, 261, 514, 571
Bme18I GGWCC 4 cut(s) 88, 871, 1013, 1307
BmeT110I CYCGRG 2 cut(s) 71, 83
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 4 cut(s) 88, 871, 1013, 1307
BmiI GGNNCC 3 cut(s) 258, 1041, 1054
BmsI GCATC 6 cut(s) 191, 255, 416, 536, 1177, 1384
BpiI GAAGAC 2 cut(s) 112, 854
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 297, 1009, 1501
BsaWI WCCGGW 2 cut(s) 184, 1273
Bsc4I CCNNNNNNNGG 1 cut(s) 1049
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 1 cut(s) 769
Bse3DI GCAATG 1 cut(s) 302
Bse8I GATNNNNATC 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 297, 1009, 1501
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 1 cut(s) 1049
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 769
BseRI GAGGAG 2 cut(s) 347, 699
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 1 cut(s) 524
BseYI CCCAGC 2 cut(s) 663, 1167
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 1052
BshTI ACCGGT 1 cut(s) 1273
BsiHKCI CYCGRG 2 cut(s) 71, 83
BsiSI CCGG 3 cut(s) 185, 254, 1274
BslFI GGGAC 1 cut(s) 1483
BslI CCNNNNNNNGG 1 cut(s) 1049
BsmFI GGGAC 1 cut(s) 1483
BsnI GGCC 1 cut(s) 114
BsoBI CYCGRG 2 cut(s) 71, 83
Bsp1286I GDGCHC 2 cut(s) 78, 496
Bsp1407I TGTACA 1 cut(s) 1492
Bsp143I GATC 5 cut(s) 532, 538, 1021, 1104, 1204
BspACI CCGC 5 cut(s) 111, 260, 570, 1348, 1451
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 258, 1041, 1054
BspPI GGATC 1 cut(s) 1099
BspQI GCTCTTC 1 cut(s) 699
BspT107I GGYRCC 1 cut(s) 1052
BsrDI GCAATG 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 1273
BsrGI TGTACA 1 cut(s) 1492
BsrI ACTGG 1 cut(s) 769
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 3 cut(s) 297, 1009, 1501
BssMI GATC 5 cut(s) 532, 538, 1021, 1104, 1204
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 1009, 1501
Bst2BI CACGAG 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 460, 911, 1497
Bst6I CTCTTC 2 cut(s) 699, 1422
BstACI GRCGYC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 1492
BstC8I GCNNGC 9 cut(s) 137, 206, 357, 470, 510, 567, 1346, 1399, 1511
BstDEI CTNAG 2 cut(s) 438, 614
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 5 cut(s) 535, 541, 1024, 1107, 1207
BstMBI GATC 5 cut(s) 532, 538, 1021, 1104, 1204
BstMWI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1350
BstSLI GKGCMC 1 cut(s) 496
BstV1I GCAGC 1 cut(s) 524
BstV2I GAAGAC 2 cut(s) 112, 854
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 214
BtrI CACGTC 1 cut(s) 118
BtsIMutI CAGTG 2 cut(s) 494, 776
Cac8I GCNNGC 9 cut(s) 137, 206, 357, 470, 510, 567, 1346, 1399, 1511
CaiI CAGNNNCTG 3 cut(s) 865, 1190, 1397
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 4 cut(s) 88, 871, 1013, 1307
CseI GACGC 3 cut(s) 154, 296, 430
Csp6I GTAC 8 cut(s) 175, 752, 857, 962, 1053, 1389, 1493, 1516
CspAI ACCGGT 1 cut(s) 1273
CviAII CATG 2 cut(s) 1135, 1225
CviQI GTAC 8 cut(s) 175, 752, 857, 962, 1053, 1389, 1493, 1516
DdeI CTNAG 2 cut(s) 438, 614
DpnI GATC 5 cut(s) 534, 540, 1023, 1106, 1206
DpnII GATC 5 cut(s) 532, 538, 1021, 1104, 1204
DraI TTTAAA 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 699, 1422
EarI CTCTTC 2 cut(s) 699, 1422
Eco130I CCWWGG 2 cut(s) 1009, 1501
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 4 cut(s) 88, 871, 1013, 1307
Eco57I CTGAAG 3 cut(s) 19, 887, 987
Eco88I CYCGRG 2 cut(s) 71, 83
EcoO109I RGGNCCY 1 cut(s) 88
EcoT14I CCWWGG 2 cut(s) 1009, 1501
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 2 cut(s) 1009, 1501
FaeI CATG 2 cut(s) 1138, 1228
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 1337, 1369
FaqI GGGAC 1 cut(s) 1483
FatI CATG 2 cut(s) 1134, 1224
FauNDI CATATG 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 112, 260, 513, 570
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 4 cut(s) 112, 260, 513, 570
FspBI CTAG 4 cut(s) 30, 536, 710, 815
GlaI GCGC 1 cut(s) 444
GluI GCNGC 4 cut(s) 112, 260, 513, 570
GsaI CCCAGC 2 cut(s) 667, 1171
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 1 cut(s) 114
HapII CCGG 3 cut(s) 185, 254, 1274
HgaI GACGC 3 cut(s) 154, 296, 430
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 1138, 1228
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 9 cut(s) 26, 143, 236, 271, 293, 643, 1154, 1195, 1244
HpaII CCGG 3 cut(s) 185, 254, 1274
HphI GGTGA 6 cut(s) 270, 689, 803, 908, 1156, 1288
Hpy188I TCNGA 6 cut(s) 298, 396, 532, 1104, 1251, 1423
Hpy188III TCNNGA 8 cut(s) 169, 311, 536, 542, 677, 887, 1199, 1310
Hpy99I CGWCG 3 cut(s) 218, 221, 312
HpyAV CCTTC 8 cut(s) 88, 157, 709, 814, 862, 919, 946, 1526
HpyCH4III ACNGT 3 cut(s) 460, 911, 1497
HpyCH4IV ACGT 2 cut(s) 117, 216
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1350
HpyF3I CTNAG 2 cut(s) 438, 614
HpySE526I ACGT 2 cut(s) 117, 216
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 1138, 1228
HspAI GCGC 1 cut(s) 443
KpnI GGTACC 1 cut(s) 1056
Kzo9I GATC 5 cut(s) 532, 538, 1021, 1104, 1204
LguI GCTCTTC 1 cut(s) 699
LmnI GCTCC 5 cut(s) 256, 334, 352, 1081, 1350
Lsp1109I GCAGC 1 cut(s) 524
LweI GCATC 6 cut(s) 191, 255, 416, 536, 1177, 1384
MaeI CTAG 4 cut(s) 30, 536, 710, 815
MaeII ACGT 2 cut(s) 117, 216
MaeIII GTNAC 3 cut(s) 905, 1369, 1540
MalI GATC 5 cut(s) 534, 540, 1023, 1106, 1206
MboI GATC 5 cut(s) 532, 538, 1021, 1104, 1204
MfeI CAATTG 2 cut(s) 7, 1364
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 78, 496
MluCI AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1364, 1488
MlyI GAGTC 4 cut(s) 20, 152, 245, 265
MseI TTAA 6 cut(s) 327, 380, 740, 1119, 1140, 1230
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 3 cut(s) 185, 254, 1274
MunI CAATTG 2 cut(s) 7, 1364
MwoI GCNNNNNNNGC 4 cut(s) 57, 205, 259, 1350
NdeI CATATG 1 cut(s) 429
NdeII GATC 5 cut(s) 532, 538, 1021, 1104, 1204
NlaIII CATG 2 cut(s) 1138, 1228
NlaIV GGNNCC 3 cut(s) 258, 1041, 1054
NmeAIII GCCGAG 2 cut(s) 202, 288
PaeR7I CTCGAG 2 cut(s) 71, 83
PciSI GCTCTTC 1 cut(s) 699
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 1544
PfeI GAWTC 5 cut(s) 293, 643, 1154, 1195, 1244
PinAI ACCGGT 1 cut(s) 1273
PkrI GCNGC 4 cut(s) 113, 261, 514, 571
PleI GAGTC 4 cut(s) 20, 151, 244, 265
PpsI GAGTC 4 cut(s) 20, 151, 244, 265
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
PspFI CCCAGC 2 cut(s) 663, 1167
PspN4I GGNNCC 3 cut(s) 258, 1041, 1054
PspPI GGNCC 4 cut(s) 88, 871, 1013, 1307
PspPPI RGGWCCY 1 cut(s) 88
PspXI VCTCGAGB 2 cut(s) 71, 83
PstNI CAGNNNCTG 3 cut(s) 865, 1190, 1397
PsuI RGATCY 1 cut(s) 538
PvuII CAGCTG 1 cut(s) 180
RsaI GTAC 8 cut(s) 176, 753, 858, 963, 1054, 1390, 1494, 1517
RsaNI GTAC 8 cut(s) 175, 752, 857, 962, 1053, 1389, 1493, 1516
SapI GCTCTTC 1 cut(s) 699
SaqAI TTAA 6 cut(s) 327, 380, 740, 1119, 1140, 1230
SatI GCNGC 4 cut(s) 112, 260, 513, 570
Sau3AI GATC 5 cut(s) 532, 538, 1021, 1104, 1204
Sau96I GGNCC 4 cut(s) 88, 871, 1013, 1307
SchI GAGTC 4 cut(s) 20, 152, 245, 265
SduI GDGCHC 2 cut(s) 78, 496
SfaNI GCATC 6 cut(s) 191, 255, 416, 536, 1177, 1384
Sfr274I CTCGAG 2 cut(s) 71, 83
SinI GGWCC 4 cut(s) 88, 871, 1013, 1307
SlaI CTCGAG 2 cut(s) 71, 83
SmlI CTYRAG 2 cut(s) 71, 83
SmoI CTYRAG 2 cut(s) 71, 83
Sse9I AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1364, 1488
SsiI CCGC 5 cut(s) 111, 260, 570, 1348, 1451
SspI AATATT 1 cut(s) 1382
SspMI CTAG 4 cut(s) 30, 536, 710, 815
StyI CCWWGG 2 cut(s) 1009, 1501
TaaI ACNGT 3 cut(s) 460, 911, 1497
TaiI ACGT 2 cut(s) 120, 219
TaqI TCGA 6 cut(s) 72, 84, 234, 543, 888, 1406
TasI AATT 9 cut(s) 7, 376, 452, 521, 758, 842, 968, 1364, 1488
TatI WGTACW 3 cut(s) 1388, 1492, 1515
TauI GCSGC 3 cut(s) 114, 262, 572
TfiI GAWTC 5 cut(s) 293, 643, 1154, 1195, 1244
Tru1I TTAA 6 cut(s) 327, 380, 740, 1119, 1140, 1230
Tru9I TTAA 6 cut(s) 327, 380, 740, 1119, 1140, 1230
TscAI CASTG 2 cut(s) 501, 776
TseI GCWGC 1 cut(s) 512
TspDTI ATGAA 8 cut(s) 94, 402, 416, 446, 561, 609, 1440, 1488
TspRI CASTG 2 cut(s) 501, 776
VpaK11BI GGWCC 4 cut(s) 88, 871, 1013, 1307
XapI RAATTY 1 cut(s) 968
XbaI TCTAGA 1 cut(s) 535
XhoI CTCGAG 2 cut(s) 71, 83
XspI CTAG 4 cut(s) 30, 536, 710, 815
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.