Rroxscaffold_2G00132180

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001668
Physical Location & Seq
Reverse (-)
69183989 .. 69188093
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132180.1

Sequence Viewer

Length: 1587 bp
ATGGTCAATTGTGACCACCACCACCACCACGACTCTAGCTTCAGCAAAGCCATCTGCTCAATCTGCCACAAAGACCTCGAGCCCTTCCTCGAGGACCTCCAAGCCATCTTCATCTGCGGCCACGTCTTCCACGAGTTCTGGTCTCTCTCTCTCTCTCTCTCGCTCTCTCTAGATTTTCTGATCGGAAATTTTTTTTGGTCTCTAAAACGGTGCGTTTTGTGTCTGCAAAACCCTAGCTTGCAAGAGTCCTTCGGCTTCTTTGAGAGCGTCAAGAGGTACAGCTGTCCGGTGTGCAAACAGCGATGCAAGCCGAACGACGTCGCTCGGCTCTATTTCGAGTCGGTGGGCATCTCGCCGGAACCGCCGAGTTTGACTCGCTCGCCGATTGGGGAAGATTCCGAGGCATTGCTTCGTGAGGTCAAAGAGCTTAAAGAGGAGCTTTATTTATGCAAGGAGCAAGCGAAGAAAGAAGTGGCTAATTTAAAGAATGAAGCTTTGAAACAACAAGCATCTATACAACAGATGCTTCATATGAAAACTGAGGCGCTTGATAAATTGACTGTGGAGTGCTTGAGGCAGCAAGAAAGGAATAGGGCATTGACCAAAGAGCTTGCAGCTTTCAAATTGGCATCTGATCTAGATCTTGATGAAAATGAGATTATGAATCTTGCCGCACTTGGTAATGGGGGAAACAATGAAAACACAATAGATGTTCTTAGAAAATCCTTGATTATGAGTAATAGGAATCGCAAAGAACTGATAGCCCAGTGCAATCTTCTTGAACGAGGAGAAGCTCTTCACCATAAAAAACTAGAGAAGGCTAAAGGAAAGATAAATAGATTAAATACAAAGGTACAGGAACTGACAGACCAGTGCAATCTTCTTGGACGAGGTGAGGCTCGTTATAGTAGAAAGCTAGAGAAGGCTAAAGGAAAGATAAACAAATTGAAGACAAAGGTACAGAAACTGAAGCACCAATGCAATCTTCTTGAACAAGGTGAGGCTCGTCACAGTAGAAAGCTAGAGAAGGCTAAAGGGAAGATAAATACATTGAAGGCAAAGGTACTAGAATTTGATGGTGTTGTTGAAGTAGAGAAAGAAACTTCAGTCCTTGGACCAAGCGATCTGTTTGAGAGTATGGTTCCTGTGAGTGGTACCTCTAATGATACTGCCAAAGCTCCTGCTGATGATATTGCAGATGTTCTGATCCTTGACGATGTTAAACAAGTCCAATCCATGCTTAACATTACAAAGGAATCTCCAATCACCCAACCACTTGCCAGACCAGATGCTGTGATTCTTGATGATCTCAACCAAGTTCAACCCATGCTTAATAGTAGCAAGGAATCTCCGATTACACAACCACTTCCCAGACCGGTGAGTGCATATTTCTCTGGTGGCTTGGTTGGTCCTGATGGAACGAAAAGGTTTTTAGGTAAATGGTGCAAGCGGAGCTTGAACAATCAATTGGTAACAAACAAATATTCAAGTACAGATGCTGGCTGTTCAATTGTTGTGGTATCTGATGAAGAGGATATTACCGAAAATACACCGCAGACAAAGAGGTGCAAGTATGAAGATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

528

Amino Acids

59.4

Weight (kDa)

8.02

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 321
AbsI CCTCGAGG 1 cut(s) 89
Acc65I GGTACC 1 cut(s) 1154
AccB1I GGYRCC 1 cut(s) 1154
AciI CCGC 5 cut(s) 117, 362, 672, 1450, 1553
AclWI GGATC 1 cut(s) 1201
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 2 cut(s) 187, 1070
AcuI CTGAAG 3 cut(s) 25, 989, 1089
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 6 cut(s) 278, 855, 960, 1065, 1156, 1492
AfiI CCNNNNNNNGG 1 cut(s) 1151
AgeI ACCGGT 1 cut(s) 1375
AjiI CACGTC 1 cut(s) 124
AjuI GAANNNNNNNTTGG 6 cut(s) 178, 210, 969, 1001, 1451, 1483
Alw26I GTCTC 2 cut(s) 147, 204
AlwI GGATC 1 cut(s) 1201
AlwNI CAGNNNCTG 4 cut(s) 862, 967, 1292, 1499
Ama87I CYCGRG 2 cut(s) 77, 89
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 2 cut(s) 577, 614
ApoI RAATTY 2 cut(s) 187, 1070
AsiGI ACCGGT 1 cut(s) 1375
Asp700I GAANNNNTTC 1 cut(s) 795
Asp718I GGTACC 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 3 cut(s) 94, 1115, 1409
AsuHPI GGTGA 5 cut(s) 791, 905, 1010, 1258, 1390
AvaI CYCGRG 2 cut(s) 77, 89
AvaII GGWCC 3 cut(s) 94, 1115, 1409
BaeI ACNNNNGTAYC 2 cut(s) 268, 301
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 84
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 2 cut(s) 118, 956
BbvI GCAGC 2 cut(s) 589, 626
BccI CCATC 4 cut(s) 59, 113, 1070, 1409
BcoDI GTCTC 2 cut(s) 147, 204
BfaI CTAG 8 cut(s) 36, 170, 234, 638, 812, 917, 1022, 1067
BfoI RGCGCY 1 cut(s) 548
BglII AGATCT 1 cut(s) 640
BisI GCNGC 4 cut(s) 118, 578, 615, 672
BlsI GCNGC 4 cut(s) 119, 579, 616, 673
Bme18I GGWCC 3 cut(s) 94, 1115, 1409
BmeT110I CYCGRG 2 cut(s) 77, 89
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 94, 1115, 1409
BmiI GGNNCC 3 cut(s) 360, 1143, 1156
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 7 cut(s) 293, 357, 513, 518, 638, 1279, 1486
BmuI ACTGGG 1 cut(s) 760
BpiI GAAGAC 2 cut(s) 118, 956
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpuEI CTTGAG 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 639
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 2 cut(s) 147, 204
BsaJI CCNNGG 2 cut(s) 399, 1111
BsaWI WCCGGW 2 cut(s) 286, 1375
Bsc4I CCNNNNNNNGG 1 cut(s) 1151
Bse118I RCCGGY 1 cut(s) 1375
Bse1I ACTGG 2 cut(s) 766, 871
Bse3DI GCAATG 1 cut(s) 404
Bse8I GATNNNNATC 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 399, 1111
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 1151
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 766, 871
BseRI GAGGAG 2 cut(s) 449, 801
BseXI GCAGC 2 cut(s) 589, 626
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 1154
BshTI ACCGGT 1 cut(s) 1375
BsiHKCI CYCGRG 2 cut(s) 77, 89
BsiSI CCGG 3 cut(s) 287, 356, 1376
BslI CCNNNNNNNGG 1 cut(s) 1151
BsmAI GTCTC 2 cut(s) 147, 204
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 2 cut(s) 147, 204
BsoBI CYCGRG 2 cut(s) 77, 89
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
BspACI CCGC 5 cut(s) 117, 362, 672, 1450, 1553
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 532
BspLI GGNNCC 3 cut(s) 360, 1143, 1156
BspPI GGATC 1 cut(s) 1201
BspQI GCTCTTC 1 cut(s) 801
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 2 cut(s) 147, 204
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 1375
BsrI ACTGG 2 cut(s) 766, 871
BssAI RCCGGY 1 cut(s) 1375
BssECI CCNNGG 2 cut(s) 399, 1111
BssMI GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 1111
Bst2BI CACGAG 1 cut(s) 131
Bst4CI ACNGT 3 cut(s) 210, 562, 1013
Bst6I CTCTTC 2 cut(s) 801, 1524
BstACI GRCGYC 1 cut(s) 318
BstC8I GCNNGC 7 cut(s) 239, 308, 380, 459, 612, 1448, 1501
BstDEI CTNAG 2 cut(s) 540, 716
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 6 cut(s) 183, 637, 643, 1126, 1209, 1309
BstMAI GTCTC 2 cut(s) 147, 204
BstMBI GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
BstMWI GCNNNNNNNGC 4 cut(s) 63, 307, 361, 1452
BstV1I GCAGC 2 cut(s) 589, 626
BstV2I GAAGAC 2 cut(s) 118, 956
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 316
BtrI CACGTC 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 773, 878
Cac8I GCNNGC 7 cut(s) 239, 308, 380, 459, 612, 1448, 1501
CaiI CAGNNNCTG 4 cut(s) 862, 967, 1292, 1499
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 1 cut(s) 1375
Cfr13I GGNCC 3 cut(s) 94, 1115, 1409
CseI GACGC 1 cut(s) 256
Csp6I GTAC 6 cut(s) 277, 854, 959, 1064, 1155, 1491
CspAI ACCGGT 1 cut(s) 1375
CspCI CAANNNNNGTGG 2 cut(s) 1497, 1532
CviAII CATG 2 cut(s) 1237, 1327
CviQI GTAC 6 cut(s) 277, 854, 959, 1064, 1155, 1491
DdeI CTNAG 2 cut(s) 540, 716
DpnI GATC 6 cut(s) 182, 636, 642, 1125, 1208, 1308
DpnII GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
DraI TTTAAA 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 801, 1524
EarI CTCTTC 2 cut(s) 801, 1524
Eco130I CCWWGG 1 cut(s) 1111
Eco24I GRGCYC 1 cut(s) 84
Eco31I GGTCTC 2 cut(s) 147, 204
Eco47I GGWCC 3 cut(s) 94, 1115, 1409
Eco57I CTGAAG 3 cut(s) 25, 989, 1089
Eco88I CYCGRG 2 cut(s) 77, 89
EcoO109I RGGNCCY 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 1111
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 1111
FaeI CATG 2 cut(s) 1240, 1330
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 1439, 1471
FatI CATG 2 cut(s) 1236, 1326
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 4 cut(s) 118, 578, 615, 672
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 4 cut(s) 118, 578, 615, 672
FspBI CTAG 8 cut(s) 36, 170, 234, 638, 812, 917, 1022, 1067
GlaI GCGC 1 cut(s) 546
GluI GCNGC 4 cut(s) 118, 578, 615, 672
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 1 cut(s) 120
HapII CCGG 3 cut(s) 287, 356, 1376
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 547
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 2 cut(s) 1240, 1330
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HindIII AAGCTT 1 cut(s) 492
HpaII CCGG 3 cut(s) 287, 356, 1376
HphI GGTGA 5 cut(s) 791, 905, 1010, 1258, 1390
Hpy188I TCNGA 7 cut(s) 180, 185, 400, 634, 1206, 1353, 1525
Hpy188III TCNNGA 9 cut(s) 170, 271, 413, 638, 644, 779, 989, 1301, 1412
Hpy99I CGWCG 2 cut(s) 320, 323
HpyAV CCTTC 6 cut(s) 94, 259, 811, 916, 1021, 1048
HpyCH4III ACNGT 3 cut(s) 210, 562, 1013
HpyCH4IV ACGT 2 cut(s) 123, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 307, 361, 1452
HpyF3I CTNAG 2 cut(s) 540, 716
HpySE526I ACGT 2 cut(s) 123, 318
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 2 cut(s) 1240, 1330
HspAI GCGC 1 cut(s) 545
KpnI GGTACC 1 cut(s) 1158
Kzo9I GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
LguI GCTCTTC 1 cut(s) 801
LmnI GCTCC 4 cut(s) 436, 454, 1183, 1452
Lsp1109I GCAGC 2 cut(s) 589, 626
LweI GCATC 7 cut(s) 293, 357, 513, 518, 638, 1279, 1486
MaeI CTAG 8 cut(s) 36, 170, 234, 638, 812, 917, 1022, 1067
MaeII ACGT 2 cut(s) 123, 318
MaeIII GTNAC 3 cut(s) 11, 1007, 1471
MalI GATC 6 cut(s) 182, 636, 642, 1125, 1208, 1308
MboI GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
MfeI CAATTG 3 cut(s) 7, 1466, 1509
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 9 cut(s) 7, 187, 478, 554, 623, 944, 1070, 1466, 1509
MlyI GAGTC 4 cut(s) 26, 254, 347, 367
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 6 cut(s) 429, 482, 842, 1221, 1242, 1332
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 3 cut(s) 287, 356, 1376
MunI CAATTG 3 cut(s) 7, 1466, 1509
MwoI GCNNNNNNNGC 4 cut(s) 63, 307, 361, 1452
NdeI CATATG 1 cut(s) 531
NdeII GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
NlaIII CATG 2 cut(s) 1240, 1330
NlaIV GGNNCC 3 cut(s) 360, 1143, 1156
NmeAIII GCCGAG 2 cut(s) 304, 390
NmuCI GTSAC 2 cut(s) 11, 1007
PaeR7I CTCGAG 2 cut(s) 77, 89
PciSI GCTCTTC 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 6 cut(s) 395, 664, 745, 1256, 1297, 1346
PinAI ACCGGT 1 cut(s) 1375
PkrI GCNGC 4 cut(s) 119, 579, 616, 673
PleI GAGTC 4 cut(s) 26, 253, 346, 367
PpsI GAGTC 4 cut(s) 26, 253, 346, 367
PpuMI RGGWCCY 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 94
PspN4I GGNNCC 3 cut(s) 360, 1143, 1156
PspPI GGNCC 3 cut(s) 94, 1115, 1409
PspPPI RGGWCCY 1 cut(s) 94
PspXI VCTCGAGB 2 cut(s) 77, 89
PstNI CAGNNNCTG 4 cut(s) 862, 967, 1292, 1499
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 282
RsaI GTAC 6 cut(s) 278, 855, 960, 1065, 1156, 1492
RsaNI GTAC 6 cut(s) 277, 854, 959, 1064, 1155, 1491
SapI GCTCTTC 1 cut(s) 801
SaqAI TTAA 6 cut(s) 429, 482, 842, 1221, 1242, 1332
SatI GCNGC 4 cut(s) 118, 578, 615, 672
Sau3AI GATC 6 cut(s) 180, 634, 640, 1123, 1206, 1306
Sau96I GGNCC 3 cut(s) 94, 1115, 1409
SchI GAGTC 4 cut(s) 26, 254, 347, 367
SduI GDGCHC 1 cut(s) 84
SfaNI GCATC 7 cut(s) 293, 357, 513, 518, 638, 1279, 1486
Sfr274I CTCGAG 2 cut(s) 77, 89
SinI GGWCC 3 cut(s) 94, 1115, 1409
SlaI CTCGAG 2 cut(s) 77, 89
SmlI CTYRAG 3 cut(s) 77, 89, 571
SmoI CTYRAG 3 cut(s) 77, 89, 571
Sse9I AATT 9 cut(s) 7, 187, 478, 554, 623, 944, 1070, 1466, 1509
SsiI CCGC 5 cut(s) 117, 362, 672, 1450, 1553
SspI AATATT 1 cut(s) 1484
SspMI CTAG 8 cut(s) 36, 170, 234, 638, 812, 917, 1022, 1067
StyI CCWWGG 1 cut(s) 1111
TaaI ACNGT 3 cut(s) 210, 562, 1013
TaiI ACGT 2 cut(s) 126, 321
TaqI TCGA 3 cut(s) 78, 90, 336
TasI AATT 9 cut(s) 7, 187, 478, 554, 623, 944, 1070, 1466, 1509
TatI WGTACW 1 cut(s) 1490
TauI GCSGC 2 cut(s) 120, 674
TfiI GAWTC 6 cut(s) 395, 664, 745, 1256, 1297, 1346
Tru1I TTAA 6 cut(s) 429, 482, 842, 1221, 1242, 1332
Tru9I TTAA 6 cut(s) 429, 482, 842, 1221, 1242, 1332
TscAI CASTG 2 cut(s) 773, 878
TseFI GTSAC 2 cut(s) 11, 1007
TseI GCWGC 2 cut(s) 577, 614
Tsp45I GTSAC 2 cut(s) 11, 1007
TspDTI ATGAA 8 cut(s) 100, 504, 518, 548, 663, 677, 711, 1542
TspRI CASTG 2 cut(s) 773, 878
VpaK11BI GGWCC 3 cut(s) 94, 1115, 1409
XapI RAATTY 2 cut(s) 187, 1070
XbaI TCTAGA 2 cut(s) 169, 637
XhoI CTCGAG 2 cut(s) 77, 89
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 8 cut(s) 36, 170, 234, 638, 812, 917, 1022, 1067
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.