RchiOBHm_Chr2g0111671

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001668
Physical Location & Seq
Forward (+)
23312996 .. 23317051
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48524

Sequence Viewer

Length: 1764 bp
ATGGACTTACTGAGTTGGTCCGTTTCAAAAGCCCAATCTGAAAACCTCTTCCCCGTCTCCCGCCCAGTCAAACCAGAACAAATTGCTTCTTCCCGCTCAGCCAGAGTCACTTTCCCTATCTTCTCCAAACCCTTCCCCAGTTTTTTTTTTTTTTTTCTCCGAGCAGATCACAAAAATCACAGTAAGAGGTTCATCGGCACCAGAGCCATGGTCAATTGCGACCACCACCAGGACTCTAGCTTCAGCAAAGCCATCTGCTCAATCTGCCACAAAGACCTCGAGCCCTTCCTCGAGGACCTCCAAGCCATCTTCATCTGCGGCCACGTCTTCCACGAGTTCTGCTTGCAAGAGTCCTTCGGCTTCTTTGAGAGCGTCAAGAGGTACAGCTGTCCGGTGTGCAAACAGCGATGCAAGCCGAACGACGTCGCTCGGCTCTATTTCGAGTCGGTGGGCATCTCGCCGGAGCCGCCGAGTTTGACTCGGTCGCCGATTGGGGAAGATTCCGAGGCATTGCGTCGTGAGGTCAAAGAGCTTAAAGAGGAGCTTTATTTATGCAAGGAGCAAGCGAAGAAAGAAGTGGCTAATTTAAAGAATGAAGCTCTGAAACAACAAGCATCTATACGACAGACGCTTCATTTGAAAACTGAGGCGCTTGATAAATTGACTGTGGAGTGCTTGCGGCTGCAAGAAAGAAATAGGGCACTGACCAAAGAGCTTGCAGCTTTCAAATTGGCATCTGATCTAGATCTCGATGAAAAGGAGATTTTGAACCTTGCCGCACTTGGTAATGGGGGAAACAATGAAAACACAATAGATGTTCTTAGAAAATCTTTGATTATGAGTAATAGGAATCGCAAAGAACTGATTGCCCAGCATATTCTTCTTGAACGAGGAGAGGCTCTTCACCGTAAAAAACTAGAGAAGGCTAAAGGAAAGATAAATAGATTGAATACAAAGGTACAGGAATTGACAGACCAGTGCAATCTTCTTGAACGAGGTGAGGCTCGTTGTAGTAGAAAGCTAGAGAAGGCTAAAGGAAAGATAAACAAATTGAAGACAAAGGTACAGAAACTGAAGGACCAATGCAATCTTCTTGAACAAGGTGAGGCTCGTTACAGTAGAAAGATAGCGAAGGCTAAAGGGAAGATAAATACATTGAAGGCAAGGGTACAAGAATTTGATGGTGCTGTTGAAGTAAGGAAAGAAACTTCAGTCCTTGGACCAAGCGATCTGTTTGAGAGTATGGTTCCTGTGAGTGGTACCTCTAATGATACTGCCAAAGCTCCTGCTGATGATATTGCAGATGTTCTGATCCTTGACGATGTTAAACAGGTACAATCCATGCTTAACATTACAAAGGAATCTCCAATCACCCAGCCACTTGCCAGACCAGATGCTGTGATTCTTGATGATCTCAACCAAGTTCAACCCATGCTTAATAGTAGCAAGGAATCTCCGATTACTCAACCACTTCCCAGACCGGTGAGTGCATATTTCTCTGGTGGCTTGATTGGTCCTGATGGAACGAAAAGGTTTTTAGGTAAATGGTGCAAGCGGAGCTTGAACAATCAATTGGTAACAAACAAATATTCAAGTACAGATGCTGGCTGTTCGATTGTTGTAGTATCTGATGAAGAGGATATTACCGAAAATACACCGCAGACAAAGAGGTGCAAGTATGAAGATAAGTCAAAAATTGTACAGTCCCAAGGGTGCTTGCAGTACAACACTTCTTTGGAAGGGTCGGTAACTACGAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

587

Amino Acids

66.06

Weight (kDa)

8.98

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
AbsI CCTCGAGG 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 1259
AccB1I GGYRCC 2 cut(s) 197, 1259
AccBSI CCGCTC 1 cut(s) 96
AciI CCGC 8 cut(s) 61, 94, 318, 467, 679, 777, 1555, 1658
AclWI GGATC 1 cut(s) 1306
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 1175
AcuI CTGAAG 3 cut(s) 226, 1094, 1194
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 9 cut(s) 383, 960, 1065, 1170, 1261, 1335, 1597, 1701, 1724
AfiI CCNNNNNNNGG 2 cut(s) 229, 1256
AgeI ACCGGT 1 cut(s) 1480
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 4 cut(s) 1074, 1106, 1556, 1588
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 1306
AlwNI CAGNNNCTG 3 cut(s) 1072, 1397, 1604
Ama87I CYCGRG 2 cut(s) 278, 290
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 682, 719
ApoI RAATTY 1 cut(s) 1175
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AsiGI ACCGGT 1 cut(s) 1480
Asp718I GGTACC 1 cut(s) 1259
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 5 cut(s) 18, 295, 1078, 1220, 1514
AsuHPI GGTGA 5 cut(s) 896, 1010, 1115, 1363, 1495
AvaI CYCGRG 2 cut(s) 278, 290
AvaII GGWCC 5 cut(s) 18, 295, 1078, 1220, 1514
BaeGI GKGCMC 1 cut(s) 703
BaeI ACNNNNGTAYC 2 cut(s) 373, 406
BanI GGYRCC 2 cut(s) 197, 1259
BanII GRGCYC 1 cut(s) 285
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 2 cut(s) 319, 1061
BbvI GCAGC 2 cut(s) 669, 731
BccI CCATC 4 cut(s) 260, 314, 1175, 1514
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 4 cut(s) 237, 743, 917, 1022
BfoI RGCGCY 1 cut(s) 653
BglII AGATCT 1 cut(s) 745
BisI GCNGC 6 cut(s) 319, 467, 680, 683, 720, 777
BlpI GCTNAGC 1 cut(s) 97
BlsI GCNGC 6 cut(s) 320, 468, 681, 684, 721, 778
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 5 cut(s) 18, 295, 1078, 1220, 1514
BmeT110I CYCGRG 2 cut(s) 278, 290
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 5 cut(s) 18, 295, 1078, 1220, 1514
BmiI GGNNCC 4 cut(s) 199, 465, 1248, 1261
BmrFI CCNGG 1 cut(s) 230
BmrI ACTGGG 2 cut(s) 59, 132
BmsI GCATC 6 cut(s) 398, 462, 623, 743, 1384, 1591
BmuI ACTGGG 2 cut(s) 59, 132
BpiI GAAGAC 2 cut(s) 319, 1061
BplI GAGNNNNNCTC 2 cut(s) 463, 495
Bpu1102I GCTNAGC 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 4 cut(s) 207, 504, 1216, 1708
BsaWI WCCGGW 2 cut(s) 391, 1480
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 1256
Bse118I RCCGGY 1 cut(s) 1480
Bse1I ACTGG 3 cut(s) 65, 138, 976
Bse3DI GCAATG 1 cut(s) 509
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 4 cut(s) 207, 504, 1216, 1708
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 2 cut(s) 229, 1256
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 111, 636
BseNI ACTGG 3 cut(s) 65, 138, 976
BseRI GAGGAG 2 cut(s) 554, 906
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 2 cut(s) 669, 731
BseYI CCCAGC 2 cut(s) 870, 1374
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 2 cut(s) 197, 1259
BshTI ACCGGT 1 cut(s) 1480
BsiEI CGRYCG 1 cut(s) 485
BsiHKCI CYCGRG 2 cut(s) 278, 290
BsiSI CCGG 3 cut(s) 392, 461, 1481
BslFI GGGAC 1 cut(s) 1690
BslI CCNNNNNNNGG 2 cut(s) 229, 1256
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 1690
BsnI GGCC 1 cut(s) 321
BsoBI CYCGRG 2 cut(s) 278, 290
Bsp1286I GDGCHC 2 cut(s) 285, 703
Bsp1407I TGTACA 1 cut(s) 1699
Bsp143I GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
Bsp1720I GCTNAGC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 8 cut(s) 61, 94, 318, 467, 679, 777, 1555, 1658
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 110, 637
BspLI GGNNCC 4 cut(s) 199, 465, 1248, 1261
BspPI GGATC 1 cut(s) 1306
BspQI GCTCTTC 1 cut(s) 906
BspT107I GGYRCC 2 cut(s) 197, 1259
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 1480
BsrGI TGTACA 1 cut(s) 1699
BsrI ACTGG 3 cut(s) 65, 138, 976
BssAI RCCGGY 1 cut(s) 1480
BssECI CCNNGG 4 cut(s) 207, 504, 1216, 1708
BssMI GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
BssNI GRCGYC 1 cut(s) 423
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 3 cut(s) 207, 1216, 1708
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 5 cut(s) 182, 667, 908, 1118, 1704
Bst6I CTCTTC 3 cut(s) 53, 906, 1629
BstACI GRCGYC 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 1699
BstC8I GCNNGC 8 cut(s) 344, 413, 564, 677, 717, 1553, 1606, 1718
BstDEI CTNAG 4 cut(s) 11, 97, 645, 821
BstDSI CCRYGG 1 cut(s) 207
BstH2I RGCGCY 1 cut(s) 653
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 6 cut(s) 169, 742, 748, 1231, 1314, 1414
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 4 cut(s) 264, 412, 466, 1557
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 228
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 669, 731
BstV2I GAAGAC 2 cut(s) 319, 1061
BstX2I RGATCY 1 cut(s) 745
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 745
BsuRI GGCC 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 421
BtrI CACGTC 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 701, 983
Cac8I GCNNGC 8 cut(s) 344, 413, 564, 677, 717, 1553, 1606, 1718
CaiI CAGNNNCTG 3 cut(s) 1072, 1397, 1604
CfoI GCGC 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 1480
Cfr13I GGNCC 5 cut(s) 18, 295, 1078, 1220, 1514
CseI GACGC 3 cut(s) 361, 503, 637
Csp6I GTAC 9 cut(s) 382, 959, 1064, 1169, 1260, 1334, 1596, 1700, 1723
CspAI ACCGGT 1 cut(s) 1480
CviAII CATG 3 cut(s) 208, 1342, 1432
CviQI GTAC 9 cut(s) 382, 959, 1064, 1169, 1260, 1334, 1596, 1700, 1723
DdeI CTNAG 4 cut(s) 11, 97, 645, 821
DpnI GATC 6 cut(s) 168, 741, 747, 1230, 1313, 1413
DpnII GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
DraI TTTAAA 1 cut(s) 588
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 3 cut(s) 53, 906, 1629
EarI CTCTTC 3 cut(s) 53, 906, 1629
Eco130I CCWWGG 3 cut(s) 207, 1216, 1708
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 5 cut(s) 18, 295, 1078, 1220, 1514
Eco57I CTGAAG 3 cut(s) 226, 1094, 1194
Eco88I CYCGRG 2 cut(s) 278, 290
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 3 cut(s) 207, 1216, 1708
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 3 cut(s) 207, 1216, 1708
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 3 cut(s) 211, 1345, 1435
FalI AAGNNNNNCTT 4 cut(s) 528, 560, 1544, 1576
FaqI GGGAC 1 cut(s) 1690
FatI CATG 3 cut(s) 207, 1341, 1431
FauI CCCGC 2 cut(s) 68, 101
Fnu4HI GCNGC 6 cut(s) 319, 467, 680, 683, 720, 777
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 6 cut(s) 319, 467, 680, 683, 720, 777
FspBI CTAG 4 cut(s) 237, 743, 917, 1022
GlaI GCGC 1 cut(s) 651
GluI GCNGC 6 cut(s) 319, 467, 680, 683, 720, 777
GsaI CCCAGC 2 cut(s) 874, 1378
HaeII RGCGCY 1 cut(s) 653
HaeIII GGCC 1 cut(s) 321
HapII CCGG 3 cut(s) 392, 461, 1481
HgaI GACGC 3 cut(s) 361, 503, 637
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 3 cut(s) 211, 1345, 1435
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HpaII CCGG 3 cut(s) 392, 461, 1481
HphI GGTGA 5 cut(s) 896, 1010, 1115, 1363, 1495
Hpy188I TCNGA 8 cut(s) 40, 161, 505, 603, 739, 1311, 1458, 1630
Hpy188III TCNNGA 9 cut(s) 376, 518, 743, 749, 884, 989, 1094, 1406, 1517
Hpy99I CGWCG 3 cut(s) 425, 428, 519
HpyAV CCTTC 9 cut(s) 142, 295, 364, 916, 1021, 1069, 1126, 1153, 1733
HpyCH4III ACNGT 5 cut(s) 182, 667, 908, 1118, 1704
HpyCH4IV ACGT 2 cut(s) 324, 423
HpyF10VI GCNNNNNNNGC 4 cut(s) 264, 412, 466, 1557
HpyF3I CTNAG 4 cut(s) 11, 97, 645, 821
HpySE526I ACGT 2 cut(s) 324, 423
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 3 cut(s) 211, 1345, 1435
HspAI GCGC 1 cut(s) 650
KpnI GGTACC 1 cut(s) 1263
Kzo9I GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 5 cut(s) 463, 541, 559, 1288, 1557
Lsp1109I GCAGC 2 cut(s) 669, 731
LweI GCATC 6 cut(s) 398, 462, 623, 743, 1384, 1591
MaeI CTAG 4 cut(s) 237, 743, 917, 1022
MaeII ACGT 2 cut(s) 324, 423
MaeIII GTNAC 4 cut(s) 106, 1112, 1576, 1747
MalI GATC 6 cut(s) 168, 741, 747, 1230, 1313, 1413
MbiI CCGCTC 1 cut(s) 96
MboI GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
MfeI CAATTG 2 cut(s) 214, 1571
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 2 cut(s) 285, 703
MlyI GAGTC 5 cut(s) 114, 227, 359, 452, 472
MseI TTAA 5 cut(s) 534, 587, 1326, 1347, 1437
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 3 cut(s) 392, 461, 1481
MspR9I CCNGG 1 cut(s) 230
MunI CAATTG 2 cut(s) 214, 1571
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 264, 412, 466, 1557
NcoI CCATGG 1 cut(s) 207
NdeII GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
NlaIII CATG 3 cut(s) 211, 1345, 1435
NlaIV GGNNCC 4 cut(s) 199, 465, 1248, 1261
NmeAIII GCCGAG 2 cut(s) 409, 495
NmuCI GTSAC 1 cut(s) 106
PaeR7I CTCGAG 2 cut(s) 278, 290
PciSI GCTCTTC 1 cut(s) 906
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 1751
PfeI GAWTC 5 cut(s) 500, 850, 1361, 1402, 1451
PflFI GACNNNGTC 1 cut(s) 481
PinAI ACCGGT 1 cut(s) 1480
PkrI GCNGC 6 cut(s) 320, 468, 681, 684, 721, 778
PleI GAGTC 5 cut(s) 113, 227, 358, 451, 472
PpsI GAGTC 5 cut(s) 113, 227, 358, 451, 472
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 228
PspFI CCCAGC 2 cut(s) 870, 1374
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 4 cut(s) 199, 465, 1248, 1261
PspPI GGNCC 5 cut(s) 18, 295, 1078, 1220, 1514
PspPPI RGGWCCY 1 cut(s) 295
PspXI VCTCGAGB 2 cut(s) 278, 290
PstNI CAGNNNCTG 3 cut(s) 1072, 1397, 1604
PsuI RGATCY 1 cut(s) 745
PsyI GACNNNGTC 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 387
RsaI GTAC 9 cut(s) 383, 960, 1065, 1170, 1261, 1335, 1597, 1701, 1724
RsaNI GTAC 9 cut(s) 382, 959, 1064, 1169, 1260, 1334, 1596, 1700, 1723
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 5 cut(s) 534, 587, 1326, 1347, 1437
SatI GCNGC 6 cut(s) 319, 467, 680, 683, 720, 777
Sau3AI GATC 6 cut(s) 166, 739, 745, 1228, 1311, 1411
Sau96I GGNCC 5 cut(s) 18, 295, 1078, 1220, 1514
SchI GAGTC 5 cut(s) 114, 227, 359, 452, 472
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 285, 703
SfaNI GCATC 6 cut(s) 398, 462, 623, 743, 1384, 1591
Sfr274I CTCGAG 2 cut(s) 278, 290
SinI GGWCC 5 cut(s) 18, 295, 1078, 1220, 1514
SlaI CTCGAG 2 cut(s) 278, 290
SmlI CTYRAG 2 cut(s) 278, 290
SmoI CTYRAG 2 cut(s) 278, 290
SsiI CCGC 8 cut(s) 61, 94, 318, 467, 679, 777, 1555, 1658
SspI AATATT 1 cut(s) 1589
SspMI CTAG 4 cut(s) 237, 743, 917, 1022
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 3 cut(s) 207, 1216, 1708
TaaI ACNGT 5 cut(s) 182, 667, 908, 1118, 1704
TaiI ACGT 2 cut(s) 327, 426
TaqI TCGA 5 cut(s) 279, 291, 441, 750, 1613
TaqII GACCGA 1 cut(s) 471
TatI WGTACW 3 cut(s) 1595, 1699, 1722
TauI GCSGC 4 cut(s) 321, 469, 682, 779
TfiI GAWTC 5 cut(s) 500, 850, 1361, 1402, 1451
Tru1I TTAA 5 cut(s) 534, 587, 1326, 1347, 1437
Tru9I TTAA 5 cut(s) 534, 587, 1326, 1347, 1437
TscAI CASTG 2 cut(s) 708, 983
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 682, 719
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 8 cut(s) 181, 301, 609, 623, 768, 816, 1647, 1695
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 708, 983
Tth111I GACNNNGTC 1 cut(s) 481
VpaK11BI GGWCC 5 cut(s) 18, 295, 1078, 1220, 1514
XapI RAATTY 1 cut(s) 1175
XbaI TCTAGA 1 cut(s) 742
XhoI CTCGAG 2 cut(s) 278, 290
XspI CTAG 4 cut(s) 237, 743, 917, 1022
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.