pycom01g09970

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001668
Physical Location & Seq
Reverse (-)
10801183 .. 10803083
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09970.3

Sequence Viewer

Length: 753 bp
ATGGTGCATTGCGGCGGCGACGGCGACGGCGACTTCTTTACCAAAACCATTTGCTCACTCTGCTGCAAAGACTTCGATCCCGTTGTCGAGGACCTCCAAGTCATCTCAATCTGCGGCCATGTCTTCCACGAACTCTGTCTGCAAGAGTCGTTCGGGTACTATGCGAACAAGAAGAGGAACAGTTGCCCGGTGTGCAAACAGAGTTGCAACCTAAACGACGTCGTCCGGGTTTATTTTCAATGGGCGGATCCGAATCTCAGTCAGAGTCAGAATCGGAGGTCGAGTGGCCAATGCGAGGAAGACTCCGAGGCGCTGCGGCAAGAGGTGAGGAGGTTGGAGGCCGTGGCGCTAGGGCTTGCTTCGGATTTGGAGCTCAAGGAGAAGGAGCTCAAATTGGTCAAAGAAGAGCTTTGTCAATCCAAGGTGCAGGCAAAGATAGAAGTTGCATTAAAGAATGAAGCCTTGAAACAGCGTACATCTGCGCAACAGCAGCTTCAAATGAAATCTGAGGAATTTGATAAATTGACTTTGGAGAGTTTAAGGTTGCGAGAAAGAAATAAGGCATTGGACAAAGAGCTTGCAGTGGTCAAATTGGTATCTGACCTAGATCTGGATAAAGAGGATGTCTCAAAGCTTGCCACTTTTGGTGATGGGGCCAACAACAAAGACACAATAGATGTTTTAAGCATTTCCTTTGCAATGTCTAATAGGAGTTGCAAGGAATGGATGAATAAATGTGGTCCTCTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

251

Amino Acids

28.23

Weight (kDa)

5.25

Isoelectric Point (pI)

59.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AatII GACGTC 1 cut(s) 222
Acc16I TGCGCA 1 cut(s) 483
AciI CCGC 5 cut(s) 12, 15, 114, 245, 316
AclWI GGATC 3 cut(s) 71, 242, 255
AcoI YGGCCR 2 cut(s) 115, 286
AcsI RAATTY 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 158, 475
AfiI CCNNNNNNNGG 2 cut(s) 295, 610
AgsI TTSAA 4 cut(s) 239, 466, 497, 749
AluBI AGCT 6 cut(s) 373, 388, 409, 493, 577, 634
AluI AGCT 6 cut(s) 373, 388, 409, 493, 577, 634
Alw21I GWGCWC 2 cut(s) 375, 390
Alw26I GTCTC 1 cut(s) 631
AlwI GGATC 3 cut(s) 71, 242, 255
AoxI GGCC 4 cut(s) 115, 286, 339, 654
ApeKI GCWGC 3 cut(s) 63, 313, 490
ApoI RAATTY 1 cut(s) 512
AspLEI GCGC 3 cut(s) 313, 349, 484
AspS9I GGNCC 3 cut(s) 91, 654, 740
AsuC2I CCSGG 2 cut(s) 188, 227
AsuHPI GGTGA 2 cut(s) 337, 659
AvaII GGWCC 2 cut(s) 91, 740
BalI TGGCCA 1 cut(s) 288
BamHI GGATCC 1 cut(s) 247
BanII GRGCYC 2 cut(s) 375, 390
BbsI GAAGAC 2 cut(s) 115, 306
Bbv12I GWGCWC 2 cut(s) 375, 390
BbvI GCAGC 3 cut(s) 50, 300, 502
BccI CCATC 1 cut(s) 644
BceAI ACGGC 3 cut(s) 37, 43, 326
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BcnI CCSGG 2 cut(s) 188, 227
BcoDI GTCTC 1 cut(s) 631
BfaI CTAG 2 cut(s) 350, 605
BfoI RGCGCY 2 cut(s) 314, 350
BglII AGATCT 1 cut(s) 607
BisI GCNGC 7 cut(s) 13, 16, 64, 115, 314, 317, 491
BlsI GCNGC 7 cut(s) 14, 17, 65, 116, 315, 318, 492
Bme1390I CCNGG 2 cut(s) 188, 227
Bme18I GGWCC 2 cut(s) 91, 740
BmgT120I GGNCC 3 cut(s) 91, 654, 740
BmiI GGNNCC 2 cut(s) 249, 655
BmrFI CCNGG 2 cut(s) 188, 227
BpiI GAAGAC 2 cut(s) 115, 306
BplI GAGNNNNNCTC 4 cut(s) 287, 319, 611, 643
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 188, 227
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 306, 342, 420
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 610
Bse3DI GCAATG 2 cut(s) 7, 705
Bse8I GATNNNNATC 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 306, 342, 420
BseGI GGATG 2 cut(s) 628, 732
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 295, 610
BseMI GCAATG 2 cut(s) 7, 705
BseMII CTCAG 2 cut(s) 271, 498
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 3 cut(s) 50, 300, 502
BsgI GTGCAG 1 cut(s) 446
BshFI GGCC 4 cut(s) 117, 288, 341, 656
BsiHKAI GWGCWC 2 cut(s) 375, 390
BsiSI CCGG 2 cut(s) 188, 226
BslI CCNNNNNNNGG 2 cut(s) 295, 610
BsmAI GTCTC 1 cut(s) 631
BsnI GGCC 4 cut(s) 117, 288, 341, 656
Bsp1286I GDGCHC 2 cut(s) 375, 390
Bsp143I GATC 3 cut(s) 76, 247, 607
BspACI CCGC 5 cut(s) 12, 15, 114, 245, 316
BspANI GGCC 4 cut(s) 117, 288, 341, 656
BspCNI CTCAG 2 cut(s) 270, 499
BspLI GGNNCC 2 cut(s) 249, 655
BspPI GGATC 3 cut(s) 71, 242, 255
BspQI GCTCTTC 1 cut(s) 399
BsrDI GCAATG 2 cut(s) 7, 705
BssECI CCNNGG 3 cut(s) 306, 342, 420
BssMI GATC 3 cut(s) 76, 247, 607
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 2 cut(s) 167, 399
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 4 cut(s) 357, 429, 579, 636
BstDEI CTNAG 2 cut(s) 257, 507
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 628, 732
BstH2I RGCGCY 2 cut(s) 314, 350
BstHHI GCGC 3 cut(s) 313, 349, 484
BstKTI GATC 3 cut(s) 79, 250, 610
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 3 cut(s) 76, 247, 607
BstMWI GCNNNNNNNGC 4 cut(s) 21, 60, 192, 490
BstSCI CCNGG 2 cut(s) 186, 225
BstV1I GCAGC 3 cut(s) 50, 300, 502
BstV2I GAAGAC 2 cut(s) 115, 306
BstX2I RGATCY 2 cut(s) 247, 607
BstYI RGATCY 2 cut(s) 247, 607
BsuRI GGCC 4 cut(s) 117, 288, 341, 656
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 628, 732
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 4 cut(s) 357, 429, 579, 636
CfoI GCGC 3 cut(s) 313, 349, 484
Cfr13I GGNCC 3 cut(s) 91, 654, 740
Csp6I GTAC 2 cut(s) 157, 474
CviAII CATG 1 cut(s) 119
CviQI GTAC 2 cut(s) 157, 474
DdeI CTNAG 2 cut(s) 257, 507
DpnI GATC 3 cut(s) 78, 249, 609
DpnII GATC 3 cut(s) 76, 247, 607
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 2 cut(s) 115, 286
Eam1104I CTCTTC 2 cut(s) 167, 399
EarI CTCTTC 2 cut(s) 167, 399
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 2 cut(s) 373, 388
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 2 cut(s) 375, 390
Eco47I GGWCC 2 cut(s) 91, 740
Eco53kI GAGCTC 2 cut(s) 373, 388
EcoICRI GAGCTC 2 cut(s) 373, 388
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 2 cut(s) 375, 390
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 1 cut(s) 122
FaiI YATR 2 cut(s) 120, 162
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 1 cut(s) 118
Fnu4HI GCNGC 7 cut(s) 13, 16, 64, 115, 314, 317, 491
FokI GGATG 2 cut(s) 635, 739
FriOI GRGCYC 2 cut(s) 375, 390
Fsp4HI GCNGC 7 cut(s) 13, 16, 64, 115, 314, 317, 491
FspBI CTAG 2 cut(s) 350, 605
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 3 cut(s) 312, 348, 483
GluI GCNGC 7 cut(s) 13, 16, 64, 115, 314, 317, 491
HaeII RGCGCY 2 cut(s) 314, 350
HaeIII GGCC 4 cut(s) 117, 288, 341, 656
HapII CCGG 2 cut(s) 188, 226
HhaI GCGC 3 cut(s) 313, 349, 484
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 3 cut(s) 311, 347, 482
HinP1I GCGC 3 cut(s) 311, 347, 482
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 146, 253, 265, 271, 302
HpaII CCGG 2 cut(s) 188, 226
HphI GGTGA 2 cut(s) 337, 659
Hpy188I TCNGA 8 cut(s) 252, 264, 270, 276, 307, 364, 508, 601
Hpy188III TCNNGA 2 cut(s) 611, 746
Hpy99I CGWCG 4 cut(s) 23, 29, 221, 224
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 60, 192, 490
HpyF3I CTNAG 2 cut(s) 257, 507
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 3 cut(s) 311, 347, 482
Kzo9I GATC 3 cut(s) 76, 247, 607
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 370, 385
LpnPI CCDG 4 cut(s) 201, 239, 413, 596
Lsp1109I GCAGC 3 cut(s) 50, 300, 502
MaeI CTAG 2 cut(s) 350, 605
MaeII ACGT 1 cut(s) 219
MalI GATC 3 cut(s) 78, 249, 609
MboI GATC 3 cut(s) 76, 247, 607
MboII GAAGA 4 cut(s) 115, 184, 311, 416
MflI RGATCY 2 cut(s) 247, 607
MhlI GDGCHC 2 cut(s) 375, 390
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 4 cut(s) 392, 512, 521, 590
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 3 cut(s) 155, 274, 296
MmeI TCCRAC 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 3 cut(s) 449, 539, 683
Msp20I TGGCCA 1 cut(s) 288
MspI CCGG 2 cut(s) 188, 226
MspR9I CCNGG 2 cut(s) 188, 227
MwoI GCNNNNNNNGC 4 cut(s) 21, 60, 192, 490
NciI CCSGG 2 cut(s) 188, 227
NdeII GATC 3 cut(s) 76, 247, 607
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 2 cut(s) 249, 655
NsbI TGCGCA 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PfeI GAWTC 2 cut(s) 253, 271
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 7 cut(s) 14, 17, 65, 116, 315, 318, 492
PleI GAGTC 3 cut(s) 154, 273, 296
PpsI GAGTC 3 cut(s) 154, 273, 296
PpuMI RGGWCCY 1 cut(s) 91
Psp124BI GAGCTC 2 cut(s) 375, 390
Psp5II RGGWCCY 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 249, 655
PspPI GGNCC 3 cut(s) 91, 654, 740
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 2 cut(s) 247, 607
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 2 cut(s) 158, 475
RsaNI GTAC 2 cut(s) 157, 474
SacI GAGCTC 2 cut(s) 375, 390
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 3 cut(s) 449, 539, 683
SatI GCNGC 7 cut(s) 13, 16, 64, 115, 314, 317, 491
Sau3AI GATC 3 cut(s) 76, 247, 607
Sau96I GGNCC 3 cut(s) 91, 654, 740
SchI GAGTC 3 cut(s) 155, 274, 296
ScrFI CCNGG 2 cut(s) 188, 227
SduI GDGCHC 2 cut(s) 375, 390
SinI GGWCC 2 cut(s) 91, 740
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 4 cut(s) 392, 512, 521, 590
SsiI CCGC 5 cut(s) 12, 15, 114, 245, 316
SspMI CTAG 2 cut(s) 350, 605
SstI GAGCTC 2 cut(s) 375, 390
StyD4I CCNGG 2 cut(s) 186, 225
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 75, 87, 281
TasI AATT 4 cut(s) 392, 512, 521, 590
TauI GCSGC 4 cut(s) 15, 18, 117, 319
TfiI GAWTC 2 cut(s) 253, 271
Tru1I TTAA 3 cut(s) 449, 539, 683
Tru9I TTAA 3 cut(s) 449, 539, 683
TscAI CASTG 1 cut(s) 588
TseI GCWGC 3 cut(s) 63, 313, 490
TspDTI ATGAA 3 cut(s) 471, 515, 743
TspRI CASTG 1 cut(s) 588
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 91, 740
XapI RAATTY 1 cut(s) 512
XspI CTAG 2 cut(s) 350, 605
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.