Prupe.2G177000_v2.0.a1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001668
Physical Location & Seq
Reverse (-)
22215515 .. 22221313
5799 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G177000.7

Sequence Viewer

Length: 1236 bp
ATGGTGAATTGCAACGCCTTTAGCAAAACCATTTGCTCACTCTGCTACAAAGATTTCGATCCCATTGTTGAGGATCTCCAAGTCATCTCCATCTGCGGCCATGTCTTCCATGAGCTCTGTCTGCAAGAGCGGTTCGGGTACTTTTCGAGCAAGAAGAAGTACAGTTGCCCGGTGTGCAAACAAAGTTGCAAGCCAAACGACGTCGCTCGGCTTTATTTTCAATGGGGTGATTCGAGTCTCACTCAGAGTCCGGTTATCGAACGCGAGGAAGATTCCGAGGCGTTGCGTCGAGAGGTGAGAAGGTTGGAGGCCATGGCGCTAGGGCTTGGTTCTGCTTTGGAGCGTAAGGAGAAGGAGGTCAAAGGGCTCAACCAGGAGCTTTATCTATCCAAGGAGCAGGTAAAAAAAGAAGTTGAATTAAAGAATGAAGCCTTGAAACAACAAACTTCCATGCAACAGCTGCTTCATATGAAATCTAAGGAATTTGATAAATTGACTTTGGAGTGCTTGAGGTTGCAAGAAAGAAATAGAGCATTGGACAAAGAGCTTGCAGCTGTTGAACTGGTATCTGATCTAGATCTGGATGAAAAGGACGTTTTAAAGATTGCAGCTCTTGGTAATGGAGCCGACAACAAAGACACAATAGATGTTCTAAGAACGTCCTTGGTGATGTCTAATAGGAATTACAAGGAATTGATGGCCAAATACAATCTACTTGAACAGAAAGAAGCTCGTCACAGTAAAAAGCTAGAGAAGGCTAGAGGAAAGATAAATAAATTGAAGACAAAGGTACAAGAATTGGAGACTGCTGTTGAAGTTAAGAATACTGAAGTTTTGAGGGCTTTAAAAGCTTCAAAGAAAACTCGAGGTGGAGGATTTATTCAGCACGGTGTAAATCAGGCAGCTTCTGATAGAAAGAAAGAAACTTCAGTTCTTGGACTGAGCAATCTGGTTGAGGCGTTGGGTTCAATAACTGGTACTTGTGCCGATACTGCCAAGACTCCTGCTGCTGATATTGCTGATGTTGTGATTATTGATGACGATGAACAAGTTCAACCCATGATGAACATCAGAAACGAATCTCCTATCCCACAACAACTTCCCAGACCAGGGGATGCATGCTTCTCTGGTGGCTTGCTTGGTCCTGATGGAACAAACAGGTATTTAGGTAAATGGTGCAAGCGGAAGCATAAGAACAGAACGGCAATAGCACAAGATCCAAGCTGTACAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

412

Amino Acids

46.11

Weight (kDa)

8.54

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 388
AccBSI CCGCTC 1 cut(s) 130
AccII CGCG 1 cut(s) 264
AciI CCGC 3 cut(s) 96, 130, 1183
AclWI GGATC 3 cut(s) 53, 81, 1211
AcoI YGGCCR 2 cut(s) 97, 699
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 2 cut(s) 849, 912
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 5 cut(s) 140, 161, 792, 979, 1228
AfiI CCNNNNNNNGG 2 cut(s) 1109, 1110
AjnI CCWGG 2 cut(s) 372, 1108
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 242, 797
AlwI GGATC 3 cut(s) 53, 81, 1211
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 3 cut(s) 97, 309, 699
ApeKI GCWGC 5 cut(s) 460, 551, 608, 902, 1007
ApoI RAATTY 1 cut(s) 482
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 1142
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 4 cut(s) 16, 239, 307, 679
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 1 cut(s) 1142
BalI TGGCCA 1 cut(s) 701
BanII GRGCYC 2 cut(s) 117, 369
BbsI GAAGAC 2 cut(s) 97, 788
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 5 cut(s) 447, 563, 620, 914, 994
BccI CCATC 3 cut(s) 98, 691, 1142
BceAI ACGGC 1 cut(s) 1218
BciT130I CCWGG 2 cut(s) 374, 1110
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 242, 797
BfaI CTAG 4 cut(s) 320, 575, 749, 759
BfoI RGCGCY 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 388
BglII AGATCT 1 cut(s) 577
BisI GCNGC 6 cut(s) 97, 461, 552, 609, 903, 1008
BlsI GCNGC 6 cut(s) 98, 462, 553, 610, 904, 1009
Bme1390I CCNGG 3 cut(s) 170, 374, 1110
Bme18I GGWCC 1 cut(s) 1142
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 1142
BmiI GGNNCC 1 cut(s) 625
BmrFI CCNGG 3 cut(s) 170, 374, 1110
BmsI GCATC 1 cut(s) 1105
BpiI GAAGAC 2 cut(s) 97, 788
BplI GAGNNNNNCTC 2 cut(s) 226, 258
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 170
BsaBI GATNNNNATC 3 cut(s) 57, 576, 1067
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 5 cut(s) 276, 312, 390, 663, 1109
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 1109, 1110
Bse1I ACTGG 2 cut(s) 567, 979
Bse8I GATNNNNATC 3 cut(s) 57, 576, 1067
BseBI CCWGG 2 cut(s) 374, 1110
BseDI CCNNGG 5 cut(s) 276, 312, 390, 663, 1109
BseGI GGATG 2 cut(s) 589, 1120
BseJI GATNNNNATC 3 cut(s) 57, 576, 1067
BseLI CCNNNNNNNGG 2 cut(s) 1109, 1110
BseMII CTCAG 2 cut(s) 257, 932
BseNI ACTGG 2 cut(s) 567, 979
BseXI GCAGC 5 cut(s) 447, 563, 620, 914, 994
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 3 cut(s) 99, 311, 701
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 2 cut(s) 170, 251
BslI CCNNNNNNNGG 2 cut(s) 1109, 1110
BsmAI GTCTC 2 cut(s) 242, 797
BsnI GGCC 3 cut(s) 99, 311, 701
BsoBI CYCGRG 1 cut(s) 864
Bsp1286I GDGCHC 2 cut(s) 117, 369
Bsp1407I TGTACA 1 cut(s) 1226
Bsp143I GATC 5 cut(s) 58, 73, 571, 577, 1216
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 3 cut(s) 96, 130, 1183
BspANI GGCC 3 cut(s) 99, 311, 701
BspCNI CTCAG 2 cut(s) 256, 933
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 625
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 3 cut(s) 53, 81, 1211
BsrBI CCGCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 1226
BsrI ACTGG 2 cut(s) 567, 979
BssECI CCNNGG 5 cut(s) 276, 312, 390, 663, 1109
BssMI GATC 5 cut(s) 58, 73, 571, 577, 1216
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 3 cut(s) 312, 390, 663
Bst2UI CCWGG 2 cut(s) 374, 1110
Bst4CI ACNGT 3 cut(s) 164, 740, 890
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 460
BstAUI TGTACA 1 cut(s) 1226
BstC8I GCNNGC 5 cut(s) 191, 549, 1120, 1136, 1181
BstDEI CTNAG 4 cut(s) 243, 477, 653, 941
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 589, 1120
BstFNI CGCG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 5 cut(s) 61, 76, 574, 580, 1219
BstMAI GTCTC 2 cut(s) 242, 797
BstMBI GATC 5 cut(s) 58, 73, 571, 577, 1216
BstMWI GCNNNNNNNGC 8 cut(s) 42, 121, 174, 460, 848, 992, 1016, 1230
BstNI CCWGG 2 cut(s) 374, 1110
BstNSI RCATGY 1 cut(s) 1122
BstSCI CCNGG 3 cut(s) 168, 372, 1108
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 5 cut(s) 447, 563, 620, 914, 994
BstV2I GAAGAC 2 cut(s) 97, 788
BstX2I RGATCY 3 cut(s) 73, 577, 1216
BstYI RGATCY 3 cut(s) 73, 577, 1216
BsuRI GGCC 3 cut(s) 99, 311, 701
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 589, 1120
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 5 cut(s) 191, 549, 1120, 1136, 1181
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 1142
CseI GACGC 1 cut(s) 275
Csp6I GTAC 5 cut(s) 139, 160, 791, 978, 1227
CviAII CATG 6 cut(s) 101, 110, 313, 451, 1060, 1119
CviQI GTAC 5 cut(s) 139, 160, 791, 978, 1227
DdeI CTNAG 4 cut(s) 243, 477, 653, 941
DpnI GATC 5 cut(s) 60, 75, 573, 579, 1218
DpnII GATC 5 cut(s) 58, 73, 571, 577, 1216
DraI TTTAAA 2 cut(s) 600, 846
EaeI YGGCCR 2 cut(s) 97, 699
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 312, 390, 663
Eco24I GRGCYC 2 cut(s) 117, 369
Eco47I GGWCC 1 cut(s) 1142
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 849, 912
Eco88I CYCGRG 1 cut(s) 864
EcoICRI GAGCTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 372, 1108
EcoT14I CCWWGG 3 cut(s) 312, 390, 663
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 2 cut(s) 117, 369
ErhI CCWWGG 3 cut(s) 312, 390, 663
FaeI CATG 6 cut(s) 104, 113, 316, 454, 1063, 1122
FaiI YATR 9 cut(s) 102, 111, 314, 452, 468, 470, 1061, 1120, 1191
FatI CATG 6 cut(s) 100, 109, 312, 450, 1059, 1118
FauNDI CATATG 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 97, 461, 552, 609, 903, 1008
FokI GGATG 2 cut(s) 596, 1127
FriOI GRGCYC 2 cut(s) 117, 369
Fsp4HI GCNGC 6 cut(s) 97, 461, 552, 609, 903, 1008
FspBI CTAG 4 cut(s) 320, 575, 749, 759
GlaI GCGC 1 cut(s) 318
GluI GCNGC 6 cut(s) 97, 461, 552, 609, 903, 1008
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 3 cut(s) 99, 311, 701
HapII CCGG 2 cut(s) 170, 251
HgaI GACGC 1 cut(s) 275
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 6 cut(s) 104, 113, 316, 454, 1063, 1122
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 6 cut(s) 230, 235, 247, 272, 1000, 1079
HpaII CCGG 2 cut(s) 170, 251
HphI GGTGA 4 cut(s) 16, 239, 307, 679
Hpy188I TCNGA 5 cut(s) 246, 277, 571, 910, 1073
Hpy188III TCNNGA 4 cut(s) 290, 575, 581, 1145
Hpy99I CGWCG 3 cut(s) 203, 206, 291
HpyAV CCTTC 3 cut(s) 294, 346, 748
HpyCH4III ACNGT 3 cut(s) 164, 740, 890
HpyCH4IV ACGT 3 cut(s) 201, 594, 659
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 121, 174, 460, 848, 992, 1016, 1230
HpyF3I CTNAG 4 cut(s) 243, 477, 653, 941
HpySE526I ACGT 3 cut(s) 201, 594, 659
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 6 cut(s) 104, 113, 316, 454, 1063, 1122
HspAI GCGC 1 cut(s) 317
Kzo9I GATC 5 cut(s) 58, 73, 571, 577, 1216
LmnI GCTCC 4 cut(s) 340, 376, 394, 623
Lsp1109I GCAGC 5 cut(s) 447, 563, 620, 914, 994
LweI GCATC 1 cut(s) 1105
MaeI CTAG 4 cut(s) 320, 575, 749, 759
MaeII ACGT 3 cut(s) 201, 594, 659
MaeIII GTNAC 1 cut(s) 734
MalI GATC 5 cut(s) 60, 75, 573, 579, 1218
MbiI CCGCTC 1 cut(s) 130
MboI GATC 5 cut(s) 58, 73, 571, 577, 1216
MboII GAAGA 4 cut(s) 97, 166, 281, 793
MflI RGATCY 3 cut(s) 73, 577, 1216
MhlI GDGCHC 2 cut(s) 117, 369
MlsI TGGCCA 1 cut(s) 701
MluCI AATT 8 cut(s) 7, 416, 482, 491, 682, 692, 776, 797
MluNI TGGCCA 1 cut(s) 701
MlyI GAGTC 3 cut(s) 244, 256, 994
MmeI TCCRAC 1 cut(s) 285
Mox20I TGGCCA 1 cut(s) 701
Mph1103I ATGCAT 1 cut(s) 1120
MroXI GAANNNNTTC 1 cut(s) 1050
MscI TGGCCA 1 cut(s) 701
MseI TTAA 4 cut(s) 419, 599, 819, 845
Msp20I TGGCCA 1 cut(s) 701
MspA1I CMGCKG 2 cut(s) 460, 554
MspI CCGG 2 cut(s) 170, 251
MspR9I CCNGG 3 cut(s) 170, 374, 1110
MvaI CCWGG 2 cut(s) 374, 1110
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 8 cut(s) 42, 121, 174, 460, 848, 992, 1016, 1230
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 468
NdeII GATC 5 cut(s) 58, 73, 571, 577, 1216
NlaIII CATG 6 cut(s) 104, 113, 316, 454, 1063, 1122
NlaIV GGNNCC 1 cut(s) 625
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 734
NsiI ATGCAT 1 cut(s) 1120
NspI RCATGY 1 cut(s) 1122
PaeI GCATGC 1 cut(s) 1122
PaeR7I CTCGAG 1 cut(s) 864
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 3 cut(s) 230, 272, 1079
PkrI GCNGC 6 cut(s) 98, 462, 553, 610, 904, 1009
PleI GAGTC 3 cut(s) 243, 255, 994
PpsI GAGTC 3 cut(s) 243, 255, 994
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 372, 1108
PspGI CCWGG 2 cut(s) 372, 1108
PspN4I GGNNCC 1 cut(s) 625
PspPI GGNCC 1 cut(s) 1142
PspXI VCTCGAGB 1 cut(s) 864
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 3 cut(s) 73, 577, 1216
PvuII CAGCTG 2 cut(s) 460, 554
RsaI GTAC 5 cut(s) 140, 161, 792, 979, 1228
RsaNI GTAC 5 cut(s) 139, 160, 791, 978, 1227
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 4 cut(s) 419, 599, 819, 845
SatI GCNGC 6 cut(s) 97, 461, 552, 609, 903, 1008
Sau3AI GATC 5 cut(s) 58, 73, 571, 577, 1216
Sau96I GGNCC 1 cut(s) 1142
SchI GAGTC 3 cut(s) 244, 256, 994
ScrFI CCNGG 3 cut(s) 170, 374, 1110
SduI GDGCHC 2 cut(s) 117, 369
SfaNI GCATC 1 cut(s) 1105
Sfr274I CTCGAG 1 cut(s) 864
SinI GGWCC 1 cut(s) 1142
SlaI CTCGAG 1 cut(s) 864
SmlI CTYRAG 2 cut(s) 508, 864
SmoI CTYRAG 2 cut(s) 508, 864
SphI GCATGC 1 cut(s) 1122
Sse9I AATT 8 cut(s) 7, 416, 482, 491, 682, 692, 776, 797
SsiI CCGC 3 cut(s) 96, 130, 1183
SspMI CTAG 4 cut(s) 320, 575, 749, 759
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 3 cut(s) 168, 372, 1108
StyI CCWWGG 3 cut(s) 312, 390, 663
TaaI ACNGT 3 cut(s) 164, 740, 890
TaiI ACGT 3 cut(s) 204, 597, 662
TaqI TCGA 6 cut(s) 57, 146, 233, 258, 289, 865
TasI AATT 8 cut(s) 7, 416, 482, 491, 682, 692, 776, 797
TatI WGTACW 2 cut(s) 159, 1226
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 3 cut(s) 230, 272, 1079
Tru1I TTAA 4 cut(s) 419, 599, 819, 845
Tru9I TTAA 4 cut(s) 419, 599, 819, 845
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 5 cut(s) 460, 551, 608, 902, 1007
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 6 cut(s) 441, 455, 485, 600, 1059, 1079
VpaK11BI GGWCC 1 cut(s) 1142
XapI RAATTY 1 cut(s) 482
XbaI TCTAGA 1 cut(s) 574
XceI RCATGY 1 cut(s) 1122
XhoI CTCGAG 1 cut(s) 864
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 4 cut(s) 320, 575, 749, 759
ZraI GACGTC 1 cut(s) 202
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.