Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0001668

Physical Location & Seq

Genomic Coordinates Reverse (-)

49978318 .. 49982366

Gene Structure 4049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG271400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGACCAATTCCAACACCGCCTTCGCCAAAACCATCTGCTCCATCTGCTACGAAGACCTCAACCCCGTCTCCGAGGATCTCCAATCCATCTCCCTCTGCGGCCACGTCTTCCACGAGCTCTGTTTGCAGCAGTGGTTCGAGTACTCGTCGAACAACAAGTGTAGTTGCCCGGTGTGCAAGCAGAGCTGCAAGGCGAGCGATGCCGGCCGGCTTTATTTCCAGTCGGTGGATCCGAGTAGCACTCAGCGACCGGTTGTTGAGTGTGAGGAGGAGGACGCCGAGGCATTGCGTCAAGAGGTGCAGAGGTTGGAGTCCAAGACGATTGTGCTTACTTCCGCATTGGACCGGCAGGGGCAGAGTCTCAAGGATCTCACCGACGAGCTTTGTTTGTGCAAGGAGCAGGCAAAGAAAGAAGTAGCATCAAAGAATGATGCAGTGGCATCAAAGAATGAAGCCTTGAAACAACAAGCATCCATGGAACGGATGCTTCGTATGAAATCTGAGGAGCTTGATAAGGTAACTATGGAGTGCTTGAGGTTGCAAGAAAAGAATATGAAATTGGCCAAGGAGCTTGCAGCATTCAAATTGGTATCTGACTTTGATCTAAAAGAAGAGGATGTCTTAAACTATGCAGCTCTTGGTAATGGGGTGAACAACAAAGAATCGATAGATAGCCTAAAAAAGTCCTTGTTGTCGCGTGAAAGGGTTTACCAGGTCCATGCCAAAAAAATGACGGCTAGATACAATGAATTGATGAAAAAGCACAGAGATCTGGCACATGAAAAGGCTCGTTCTAGTGAAGAACTAGAGAAGGCTAAAGAAAAGATGAATACATTAAAGACAAGGGTACAAGAATTGGAGAGTGCGGTTGAAGTCAAGGACAATGAAGTTTTGAGGGCTTTAAAAGCTTCAAGGGAAACTCGAGGGGAAAGAGTTATTCAGAATAATGTTAGTTGTAAATCTGATTCTGTGTCCGTCAACAATTCATCAGAAGATCAAAGCAAACAGTTTGCTCCAATGCATAAATTTGATAGGATAGGAAGCTTGCCAAATGATCCACTGTGCCAGACAGTACACTTCAATTTCATTAGCCCTATGGATGCAAATTCTACTAAAGATGGTACAAGTACCAGTGCTGATGGTGAAGAAAGAGATCCTTACCCTTTAATTGATGATGATGCTTCAAAATTTTCCACAACTAGACATGAACTTTCAAATGCCAATGTGAAAAACCCACTCTGTGAACATAGAACTAGCCAGAAGTCTAGTATGTTGAGACCAGGGGCTGCTTCTGATTCAATCAATGAAACTTCAGTCTGTAGACTAAGAAATCTGGACGAGCCTTTGGGCTCAATAACTGGTAAAATGACTCATATTTCCAAGTCTCCTGCTGCTGATGTTGTGATTCTTGATGATGTAGAAGAAGTCTCCCCGGTGCTTAACATTATAAAGGAATCTCCCATCACACAAGCACTTCCCAGACAAGGGGTTGCAGGCTCTTCTGGTGGCTTGCTTGGTCTTGATGGAGCAAACAGGTTGGGAAAATGGTGCAAGCAGAGTGTAAACAATGGATCTGTAACAAGGCAAGACTCAACGGCAGATAAAAGTGATTTGATTGCTGTAGGAACTGACGGAAGAGGTGGAAGAATCAAAGTTCTGAGATCTCATAATCAATCAACATTGGTGAGGTCATCTAGTGTTATTTGTTTTATTCTGGTCCAAAATGCTTTATCAGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 map00053 map01100

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

580

Amino Acids

64.31

Weight (kDa)

6.1

Isoelectric Point (pI)

50.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	12 - 60	1e-08	Ring finger domain
zf-RING_UBOX	PF13445	13 - 57	2.3e-07	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001668)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01245 AT4G01270
fragaria_vesca	FvH4_1g19860 FvH4_1g19860 FvH4_1g19860 FvH4_1g19860 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320
malus_domestica	MD01G1070500.v1.1 MD01G1070600.v1.1 MD01G1071100.v1.1
prunus_persica	Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177100_v2.0.a1 Prupe.2G177100_v2.0.a1
pyrus_communis	pycom01g09970 pycom01g10000 pycom07g12860
rosa_chinensis	RchiOBHm_Chr1g0357651 RchiOBHm_Chr2g0111671
rosa_laevigata	RLG00000017916 RLG00000028011
rosa_multiflora	Rmu_sc0004683.1_g000004 Rmu_sc0005791.1_g000023
rosa_roxburghii	Rroxscaffold_2G00132180 Rroxscaffold_4G00298670
rosa_rugosa	Rorug01G0257400 Rorug02G0175300 Rorug02G0175400
rosa_samantha	Rh1AG271400 Rh1BG238300 Rh1CG255200 Rh1DG266600 Rh2AG228300 Rh2BG240500 Rh2CG231700 Rh2DG236300
rosa_wichuraiana	Rw1G024190 Rw2G017620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1448
AccB7I	CCANNNNNTGG	1 cut(s)	226
AccI	GTMKAC	1 cut(s)	1321
AccII	CGCG	1 cut(s)	697
AciI	CCGC	4 cut(s)	18, 99, 336, 866
AclWI	GGATC	7 cut(s)	84, 224, 237, 375, 1049, 1148, 1579
AcoI	YGGCCR	3 cut(s)	100, 205, 561
AcsI	RAATTY	3 cut(s)	1025, 1105, 1187
AcuI	CTGAAG	1 cut(s)	1296
AcyI	GRCGYC	1 cut(s)	276
AdeI	CACNNNGTG	1 cut(s)	1241
AfaI	GTAC	5 cut(s)	143, 849, 1074, 1123, 1129
AfiI	CCNNNNNNNGG	4 cut(s)	226, 480, 1484, 1485
AgeI	ACCGGT	1 cut(s)	250
AgsI	TTSAA	8 cut(s)	460, 583, 872, 912, 1081, 1185, 1215, 1299
AjiI	CACGTC	1 cut(s)	106
AjnI	CCWGG	2 cut(s)	711, 1279
AjuI	GAANNNNNNNTTGG	3 cut(s)	37, 542, 574
AluBI	AGCT	8 cut(s)	118, 186, 382, 508, 571, 635, 908, 1044
AluI	AGCT	8 cut(s)	118, 186, 382, 508, 571, 635, 908, 1044
Alw21I	GWGCWC	1 cut(s)	120
Alw26I	GTCTC	5 cut(s)	73, 365, 1270, 1389, 1432
AlwI	GGATC	7 cut(s)	84, 224, 237, 375, 1049, 1148, 1579
AlwNI	CAGNNNCTG	1 cut(s)	1286
Ama87I	CYCGRG	1 cut(s)	921
AoxI	GGCC	3 cut(s)	100, 205, 561
ApeKI	GCWGC	6 cut(s)	127, 186, 575, 632, 1286, 1391
ApoI	RAATTY	3 cut(s)	1025, 1105, 1187
AsiGI	ACCGGT	1 cut(s)	250
AspS9I	GGNCC	3 cut(s)	343, 715, 1717
AsuC2I	CCSGG	2 cut(s)	170, 1433
AsuHPI	GGTGA	4 cut(s)	364, 661, 1154, 1696
AvaI	CYCGRG	1 cut(s)	921
AvaII	GGWCC	3 cut(s)	343, 715, 1717
BalI	TGGCCA	1 cut(s)	563
BamHI	GGATCC	1 cut(s)	229
BanII	GRGCYC	2 cut(s)	120, 1352
BauI	CACGAG	1 cut(s)	113
BbsI	GAAGAC	2 cut(s)	60, 100
Bbv12I	GWGCWC	1 cut(s)	120
BbvI	GCAGC	6 cut(s)	139, 173, 587, 644, 1273, 1378
BccI	CCATC	7 cut(s)	41, 50, 95, 1112, 1133, 1469, 1517
BceAI	ACGGC	2 cut(s)	750, 1611
BciT130I	CCWGG	2 cut(s)	713, 1281
BcnI	CCSGG	2 cut(s)	170, 1433
BcoDI	GTCTC	5 cut(s)	73, 365, 1270, 1389, 1432
BfaI	CTAG	7 cut(s)	738, 795, 806, 1200, 1254, 1266, 1695
BfmI	CTRYAG	2 cut(s)	1318, 1620
BglII	AGATCT	2 cut(s)	769, 1661
BisI	GCNGC	7 cut(s)	100, 128, 187, 576, 633, 1287, 1392
BlsI	GCNGC	7 cut(s)	101, 129, 188, 577, 634, 1288, 1393
BmcAI	AGTACT	1 cut(s)	143
Bme1390I	CCNGG	4 cut(s)	170, 713, 1281, 1433
Bme18I	GGWCC	3 cut(s)	343, 715, 1717
BmeT110I	CYCGRG	1 cut(s)	921
BmgBI	CACGTC	1 cut(s)	106
BmgT120I	GGNCC	3 cut(s)	343, 715, 1717
BmiI	GGNNCC	1 cut(s)	231
BmrFI	CCNGG	4 cut(s)	170, 713, 1281, 1433
BmsI	GCATC	8 cut(s)	190, 421, 428, 449, 474, 479, 1090, 1168
BpiI	GAAGAC	2 cut(s)	60, 100
BplI	GAGNNNNNCTC	2 cut(s)	226, 258
BpuEI	CTTGAG	2 cut(s)	347, 553
BpuMI	CCSGG	2 cut(s)	170, 1433
Bsa29I	ATCGAT	1 cut(s)	665
BsaHI	GRCGYC	1 cut(s)	276
BsaI	GGTCTC	1 cut(s)	1270
BsaJI	CCNNGG	6 cut(s)	72, 279, 474, 564, 1280, 1431
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	53, 83
Bsc4I	CCNNNNNNNGG	4 cut(s)	226, 480, 1484, 1485
Bse118I	RCCGGY	4 cut(s)	203, 207, 250, 345
Bse1I	ACTGG	4 cut(s)	220, 1131, 1363, 1742
Bse3DI	GCAATG	1 cut(s)	284
BseBI	CCWGG	2 cut(s)	713, 1281
BseCI	ATCGAT	1 cut(s)	665
BseDI	CCNNGG	6 cut(s)	72, 279, 474, 564, 1280, 1431
BseGI	GGATG	4 cut(s)	470, 489, 622, 1105
BseLI	CCNNNNNNNGG	4 cut(s)	226, 480, 1484, 1485
BseMI	GCAATG	1 cut(s)	284
BseMII	CTCAG	3 cut(s)	257, 492, 1649
BseNI	ACTGG	4 cut(s)	220, 1131, 1363, 1742
BseRI	GAGGAG	3 cut(s)	281, 284, 518
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	6 cut(s)	139, 173, 587, 644, 1273, 1378
BsgI	GTGCAG	1 cut(s)	320
Bsh1236I	CGCG	1 cut(s)	697
Bsh1285I	CGRYCG	2 cut(s)	208, 251
BshFI	GGCC	3 cut(s)	102, 207, 563
BshTI	ACCGGT	1 cut(s)	250
BshVI	ATCGAT	1 cut(s)	665
BsiEI	CGRYCG	2 cut(s)	208, 251
BsiHKAI	GWGCWC	1 cut(s)	120
BsiHKCI	CYCGRG	1 cut(s)	921
BsiSI	CCGG	6 cut(s)	170, 204, 208, 251, 346, 1433
BslI	CCNNNNNNNGG	4 cut(s)	226, 480, 1484, 1485
BsmAI	GTCTC	5 cut(s)	73, 365, 1270, 1389, 1432
BsmBI	CGTCTC	1 cut(s)	73
BsmI	GAATGC	1 cut(s)	578
BsnI	GGCC	3 cut(s)	102, 207, 563
Bso31I	GGTCTC	1 cut(s)	1270
BsoBI	CYCGRG	1 cut(s)	921
Bsp1286I	GDGCHC	2 cut(s)	120, 1352
Bsp19I	CCATGG	1 cut(s)	474
BspACI	CCGC	4 cut(s)	18, 99, 336, 866
BspANI	GGCC	3 cut(s)	102, 207, 563
BspCNI	CTCAG	3 cut(s)	256, 493, 1650
BspDI	ATCGAT	1 cut(s)	665
BspFNI	CGCG	1 cut(s)	697
BspLI	GGNNCC	1 cut(s)	231
BspPI	GGATC	7 cut(s)	84, 224, 237, 375, 1049, 1148, 1579
BspQI	GCTCTTC	1 cut(s)	1504
BspTNI	GGTCTC	1 cut(s)	1270
BsrDI	GCAATG	1 cut(s)	284
BsrFI	RCCGGY	4 cut(s)	203, 207, 250, 345
BsrI	ACTGG	4 cut(s)	220, 1131, 1363, 1742
BssAI	RCCGGY	4 cut(s)	203, 207, 250, 345
BssECI	CCNNGG	6 cut(s)	72, 279, 474, 564, 1280, 1431
BssNI	GRCGYC	1 cut(s)	276
BssSI	CACGAG	1 cut(s)	113
BssT1I	CCWWGG	2 cut(s)	474, 564
Bst2BI	CACGAG	1 cut(s)	113
Bst2UI	CCWGG	2 cut(s)	713, 1281
Bst4CI	ACNGT	3 cut(s)	1008, 1062, 1072
Bst6I	CTCTTC	3 cut(s)	606, 1504, 1630
BstACI	GRCGYC	1 cut(s)	276
BstDEI	CTNAG	4 cut(s)	243, 501, 1325, 1658
BstDSI	CCRYGG	1 cut(s)	474
BstF5I	GGATG	4 cut(s)	470, 489, 622, 1105
BstFNI	CGCG	1 cut(s)	697
BstMAI	GTCTC	5 cut(s)	73, 365, 1270, 1389, 1432
BstMCI	CGRYCG	2 cut(s)	208, 251
BstMWI	GCNNNNNNNGC	8 cut(s)	45, 124, 174, 183, 195, 200, 204, 905
BstNI	CCWGG	2 cut(s)	713, 1281
BstSCI	CCNGG	4 cut(s)	168, 711, 1279, 1431
BstSFI	CTRYAG	2 cut(s)	1318, 1620
BstUI	CGCG	1 cut(s)	697
BstV1I	GCAGC	6 cut(s)	139, 173, 587, 644, 1273, 1378
BstV2I	GAAGAC	2 cut(s)	60, 100
BstX2I	RGATCY	7 cut(s)	76, 229, 367, 769, 1153, 1571, 1661
BstYI	RGATCY	7 cut(s)	76, 229, 367, 769, 1153, 1571, 1661
BstZI	CGGCCG	1 cut(s)	205
Bsu15I	ATCGAT	1 cut(s)	665
BsuRI	GGCC	3 cut(s)	102, 207, 563
BsuTUI	ATCGAT	1 cut(s)	665
BtgI	CCRYGG	1 cut(s)	474
BtgZI	GCGATG	1 cut(s)	213
BtrI	CACGTC	1 cut(s)	106
BtsCI	GGATG	4 cut(s)	470, 489, 622, 1105
BtsI	GCAGTG	2 cut(s)	137, 441
BtsIMutI	CAGTG	4 cut(s)	137, 441, 1058, 1138
CaiI	CAGNNNCTG	1 cut(s)	1286
Cfr10I	RCCGGY	4 cut(s)	203, 207, 250, 345
Cfr13I	GGNCC	3 cut(s)	343, 715, 1717
ClaI	ATCGAT	1 cut(s)	665
CseI	GACGC	2 cut(s)	278, 284
CsiI	ACCWGGT	1 cut(s)	711
Csp6I	GTAC	5 cut(s)	142, 848, 1073, 1122, 1128
CspAI	ACCGGT	1 cut(s)	250
CviAII	CATG	4 cut(s)	475, 719, 779, 1205
CviQI	GTAC	5 cut(s)	142, 848, 1073, 1122, 1128
DdeI	CTNAG	4 cut(s)	243, 501, 1325, 1658
DraI	TTTAAA	1 cut(s)	903
DraIII	CACNNNGTG	1 cut(s)	1241
EaeI	YGGCCR	3 cut(s)	100, 205, 561
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	3 cut(s)	606, 1504, 1630
EarI	CTCTTC	3 cut(s)	606, 1504, 1630
Ecl136II	GAGCTC	1 cut(s)	118
EclXI	CGGCCG	1 cut(s)	205
Eco130I	CCWWGG	2 cut(s)	474, 564
Eco24I	GRGCYC	2 cut(s)	120, 1352
Eco31I	GGTCTC	1 cut(s)	1270
Eco47I	GGWCC	3 cut(s)	343, 715, 1717
Eco52I	CGGCCG	1 cut(s)	205
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	1 cut(s)	1296
Eco88I	CYCGRG	1 cut(s)	921
EcoICRI	GAGCTC	1 cut(s)	118
EcoRII	CCWGG	2 cut(s)	711, 1279
EcoT14I	CCWWGG	2 cut(s)	474, 564
EcoT22I	ATGCAT	1 cut(s)	1023
EcoT38I	GRGCYC	2 cut(s)	120, 1352
ErhI	CCWWGG	2 cut(s)	474, 564
Esp3I	CGTCTC	1 cut(s)	73
FaeI	CATG	4 cut(s)	478, 722, 782, 1208
FalI	AAGNNNNNCTT	2 cut(s)	1141, 1173
FatI	CATG	4 cut(s)	474, 718, 778, 1204
FblI	GTMKAC	1 cut(s)	1321
Fnu4HI	GCNGC	7 cut(s)	100, 128, 187, 576, 633, 1287, 1392
FokI	GGATG	4 cut(s)	457, 496, 629, 1112
FriOI	GRGCYC	2 cut(s)	120, 1352
Fsp4HI	GCNGC	7 cut(s)	100, 128, 187, 576, 633, 1287, 1392
FspBI	CTAG	7 cut(s)	738, 795, 806, 1200, 1254, 1266, 1695
GluI	GCNGC	7 cut(s)	100, 128, 187, 576, 633, 1287, 1392
HaeIII	GGCC	3 cut(s)	102, 207, 563
HapII	CCGG	6 cut(s)	170, 204, 208, 251, 346, 1433
HgaI	GACGC	2 cut(s)	278, 284
Hin1I	GRCGYC	1 cut(s)	276
Hin1II	CATG	4 cut(s)	478, 722, 782, 1208
HincII	GTYRAC	1 cut(s)	979
HindII	GTYRAC	1 cut(s)	979
HindIII	AAGCTT	2 cut(s)	906, 1042
HpaII	CCGG	6 cut(s)	170, 204, 208, 251, 346, 1433
HphI	GGTGA	4 cut(s)	364, 661, 1154, 1696
Hpy166II	GTNNAC	7 cut(s)	652, 709, 979, 1075, 1244, 1322, 1564
Hpy188III	TCNNGA	4 cut(s)	293, 1334, 1409, 1520
Hpy8I	GTNNAC	7 cut(s)	652, 709, 979, 1075, 1244, 1322, 1564
Hpy99I	CGWCG	2 cut(s)	151, 380
HpyAV	CCTTC	2 cut(s)	31, 805
HpyCH4III	ACNGT	3 cut(s)	1008, 1062, 1072
HpyCH4IV	ACGT	1 cut(s)	105
HpyF10VI	GCNNNNNNNGC	8 cut(s)	45, 124, 174, 183, 195, 200, 204, 905
HpyF3I	CTNAG	4 cut(s)	243, 501, 1325, 1658
HpySE526I	ACGT	1 cut(s)	105
Hsp92I	GRCGYC	1 cut(s)	276
Hsp92II	CATG	4 cut(s)	478, 722, 782, 1208
KroI	GCCGGC	2 cut(s)	203, 207
KroNI	GCCGGC	2 cut(s)	205, 209
LguI	GCTCTTC	1 cut(s)	1504
LmnI	GCTCC	6 cut(s)	44, 397, 505, 568, 1018, 1526
Lsp1109I	GCAGC	6 cut(s)	139, 173, 587, 644, 1273, 1378
LweI	GCATC	8 cut(s)	190, 421, 428, 449, 474, 479, 1090, 1168
MabI	ACCWGGT	1 cut(s)	711
MaeI	CTAG	7 cut(s)	738, 795, 806, 1200, 1254, 1266, 1695
MaeII	ACGT	1 cut(s)	105
MaeIII	GTNAC	2 cut(s)	517, 1576
MflI	RGATCY	7 cut(s)	76, 229, 367, 769, 1153, 1571, 1661
MhlI	GDGCHC	2 cut(s)	120, 1352
MlsI	TGGCCA	1 cut(s)	563
MluNI	TGGCCA	1 cut(s)	563
MlyI	GAGTC	4 cut(s)	320, 367, 1363, 1583
MmeI	TCCRAC	2 cut(s)	36, 288
Mox20I	TGGCCA	1 cut(s)	563
Mph1103I	ATGCAT	1 cut(s)	1023
MroNI	GCCGGC	2 cut(s)	203, 207
MscI	TGGCCA	1 cut(s)	563
MseI	TTAA	5 cut(s)	623, 836, 902, 1166, 1440
Msp20I	TGGCCA	1 cut(s)	563
MspI	CCGG	6 cut(s)	170, 204, 208, 251, 346, 1433
MspR9I	CCNGG	4 cut(s)	170, 713, 1281, 1433
Mva1269I	GAATGC	1 cut(s)	578
MvaI	CCWGG	2 cut(s)	713, 1281
MvnI	CGCG	1 cut(s)	697
MwoI	GCNNNNNNNGC	8 cut(s)	45, 124, 174, 183, 195, 200, 204, 905
NaeI	GCCGGC	2 cut(s)	205, 209
NciI	CCSGG	2 cut(s)	170, 1433
NcoI	CCATGG	1 cut(s)	474
NgoMIV	GCCGGC	2 cut(s)	203, 207
NlaIII	CATG	4 cut(s)	478, 722, 782, 1208
NlaIV	GGNNCC	1 cut(s)	231
NmeAIII	GCCGAG	1 cut(s)	304
NsiI	ATGCAT	1 cut(s)	1023
PaeR7I	CTCGAG	1 cut(s)	921
PciSI	GCTCTTC	1 cut(s)	1504
PcsI	WCGNNNNNNNCGW	3 cut(s)	111, 230, 487
PctI	GAATGC	1 cut(s)	578
PdiI	GCCGGC	2 cut(s)	205, 209
PfeI	GAWTC	6 cut(s)	662, 965, 1295, 1405, 1454, 1647
PflMI	CCANNNNNTGG	1 cut(s)	226
PinAI	ACCGGT	1 cut(s)	250
PkrI	GCNGC	7 cut(s)	101, 129, 188, 577, 634, 1288, 1393
PleI	GAGTC	4 cut(s)	319, 366, 1363, 1583
PpsI	GAGTC	4 cut(s)	319, 366, 1363, 1583
PsiI	TTATAA	1 cut(s)	1448
Psp124BI	GAGCTC	1 cut(s)	120
Psp6I	CCWGG	2 cut(s)	711, 1279
PspGI	CCWGG	2 cut(s)	711, 1279
PspN4I	GGNNCC	1 cut(s)	231
PspPI	GGNCC	3 cut(s)	343, 715, 1717
PspXI	VCTCGAGB	1 cut(s)	921
PstNI	CAGNNNCTG	1 cut(s)	1286
PsuI	RGATCY	7 cut(s)	76, 229, 367, 769, 1153, 1571, 1661
RsaI	GTAC	5 cut(s)	143, 849, 1074, 1123, 1129
RsaNI	GTAC	5 cut(s)	142, 848, 1073, 1122, 1128
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	1504
SaqAI	TTAA	5 cut(s)	623, 836, 902, 1166, 1440
SatI	GCNGC	7 cut(s)	100, 128, 187, 576, 633, 1287, 1392
Sau96I	GGNCC	3 cut(s)	343, 715, 1717
ScaI	AGTACT	1 cut(s)	143
SchI	GAGTC	4 cut(s)	320, 367, 1363, 1583
ScrFI	CCNGG	4 cut(s)	170, 713, 1281, 1433
SduI	GDGCHC	2 cut(s)	120, 1352
SexAI	ACCWGGT	1 cut(s)	711
SfaNI	GCATC	8 cut(s)	190, 421, 428, 449, 474, 479, 1090, 1168
SfcI	CTRYAG	2 cut(s)	1318, 1620
Sfr274I	CTCGAG	1 cut(s)	921
SinI	GGWCC	3 cut(s)	343, 715, 1717
SlaI	CTCGAG	1 cut(s)	921
SmlI	CTYRAG	3 cut(s)	362, 532, 921
SmoI	CTYRAG	3 cut(s)	362, 532, 921
SsiI	CCGC	4 cut(s)	18, 99, 336, 866
SspMI	CTAG	7 cut(s)	738, 795, 806, 1200, 1254, 1266, 1695
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	4 cut(s)	168, 711, 1279, 1431
StyI	CCWWGG	2 cut(s)	474, 564
TaaI	ACNGT	3 cut(s)	1008, 1062, 1072
TaiI	ACGT	1 cut(s)	108
TaqI	TCGA	4 cut(s)	138, 149, 665, 922
TatI	WGTACW	2 cut(s)	141, 1072
TauI	GCSGC	1 cut(s)	102
TfiI	GAWTC	6 cut(s)	662, 965, 1295, 1405, 1454, 1647
Tru1I	TTAA	5 cut(s)	623, 836, 902, 1166, 1440
Tru9I	TTAA	5 cut(s)	623, 836, 902, 1166, 1440
TscAI	CASTG	4 cut(s)	137, 441, 1065, 1138
TseI	GCWGC	6 cut(s)	127, 186, 575, 632, 1286, 1391
TspGWI	ACGGA	3 cut(s)	496, 964, 1647
TspRI	CASTG	4 cut(s)	137, 441, 1065, 1138
Van91I	CCANNNNNTGG	1 cut(s)	226
VpaK11BI	GGWCC	3 cut(s)	343, 715, 1717
XapI	RAATTY	3 cut(s)	1025, 1105, 1187
XhoI	CTCGAG	1 cut(s)	921
XmiI	GTMKAC	1 cut(s)	1321
XspI	CTAG	7 cut(s)	738, 795, 806, 1200, 1254, 1266, 1695
ZrmI	AGTACT	1 cut(s)	143
Zsp2I	ATGCAT	1 cut(s)	1023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.