Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001668

Physical Location & Seq

Genomic Coordinates Reverse (-)

17520930 .. 17524673

Gene Structure 3744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1070600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGGCCAATGCAGATTCCCACAATTTTACCAAAACAATCTGCTCAATCTGCTACGAAGACCTCAAGCCCACTTCTGAGGATCTCCAATCCATCTCCATCTGCGGCCATGTCTTCCACGAGCTCTGTCTGCAACAGTGGTTCGAGTACTCTGCGAGGACGAAGAAGTACACTTGCCCGGTGTGCAAACAAAATTGCAAGCAAAAGGATGCCGGCCGGCTTTATTTTCAGTCGGTGGGGGACGCGGCGGATGCGACTCAGAGGCCGATTAGCAAATGCGAGGAAGATCCCCAGGTGTTGCGTCGAGAGGTGAAAAGGTTGGAGGTGGCGGAGCTGGGAATTCGTTTGGCTTTCGATCTTCAGGGGAAGGAGCTTAAGGAGCTCAAAGAGGAGCTTTGTCAATGTAAGGAGGTGGCACAGAAAGAAGTTGTCTTGAAAAATGAAGCCTTGAAACAACAAGCATACATGCAGCTACAGCTTACGATGAAAACTGAGGCTCTTGATAAATCGACCATGGAGCGTTTGAGGTTGCAAGAAAGAAACATGGCATTGGCCAAAGAGCTTGCAGCATTCAAACTGGTATCTGATCTAGATCTGGATGAAGATGAGGTTTCAAAGCTTTCCACTCTTGGTAATGGTGCCAACACCAAAGACACGATAGATATTCTTAGAAAGTCCTTGGAGATGAGTAAAAGGAATTACAAAGAATTGATAAACAAATACAATGGTCTTGCACGAGAAGCTCGTGAGAGCAAAAAACTGGAGCAGGAGGCTAAAGAAAAGATTAAAAAAATGAAGACAAGGGTACAAGAAATGGAGACGGTTGTCGAAGTAAAAGATAATGAAGTTTTGAGGGCTTTAAAAGCTTCAAAGACAACTCGGGGTGAAGGAATTATGCAGAATGGTGAAAGATGTACCACTAATTCAGTGTCTGTCAACAATTCATCAGAAGTTCAAAGAAAACATCCGTATGCACCCATTCGTAAGTTGGATAGAATTGAAAAATGGGAAAATGATTTACTGTGTCATAGGAAAACAGAAAACTCAAACTTCACTGACGATATGGATTTAAGCTGTACTAAAGATGGTACAAGCACCACTGTTCATGATAAAGTAAGAGATGCCTACTCTTTAGGAGATAAAGGTGCTTCAAAACTTTCTACAGCTAAGCGTGGACTTTCAAATCCCAAATTGAAAGAACCAACCTATGAGGATGTTGTTAACCAGAAGTGCACTCGGTTGAGGTCAGATGCAGTTTCTGATACTAGGAAAGAAACTCCTACTTGTAACAATACTGCCAGGACTCCTGCTGCCGATGTTGTGATTCTTGATGATGTTGAACAAGATCAACCCATGTTTAACATTAGAAAGGAGTCTCCTGTCCCACAACCACTTCCCGGAACAGGGGATGTATGTTTTTCTGGTGGCTTGCTTGGGCCTGATGGAACAAACAGGTTTTTAGGTAAATGGTGCAAGCGGGGTCTGAACAAGGGATCAGAATCAAGACAAAACCCAAGTGCGGATTCTGGTAATTTGATTGCTGTAGGAGCTGACGGAAGAGGTGGAAGAATCAAAGTTCTTAGATCACTGAATCATTCAACAGTGGATAATAAGGAGAATGCACCGGCGACAAAGCGCTTCAAGTCGGGGTCTAAAGCAAATAGTGTGCAGTCCCAAGGGTGCTTGCAAATAGAACACTTCTTTGGGAGGGTTGGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 map00053 map01100

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

573

Amino Acids

64.21

Weight (kDa)

8.8

Isoelectric Point (pI)

39.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	13 - 62	1.9e-09	Ring finger domain
zf-RING_UBOX	PF13445	14 - 59	9.9e-08	RING-type zinc-finger
zf-C3HC4	PF00097	14 - 61	3.3e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	14 - 63	3.4e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001668)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01245 AT4G01270
fragaria_vesca	FvH4_1g19860 FvH4_1g19860 FvH4_1g19860 FvH4_1g19860 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320 FvH4_7g15320
malus_domestica	MD01G1070500.v1.1 MD01G1070600.v1.1 MD01G1071100.v1.1
prunus_persica	Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177000_v2.0.a1 Prupe.2G177100_v2.0.a1 Prupe.2G177100_v2.0.a1
pyrus_communis	pycom01g09970 pycom01g10000 pycom07g12860
rosa_chinensis	RchiOBHm_Chr1g0357651 RchiOBHm_Chr2g0111671
rosa_laevigata	RLG00000017916 RLG00000028011
rosa_multiflora	Rmu_sc0004683.1_g000004 Rmu_sc0005791.1_g000023
rosa_roxburghii	Rroxscaffold_2G00132180 Rroxscaffold_4G00298670
rosa_rugosa	Rorug01G0257400 Rorug02G0175300 Rorug02G0175400
rosa_samantha	Rh1AG271400 Rh1BG238300 Rh1CG255200 Rh1DG266600 Rh2AG228300 Rh2BG240500 Rh2CG231700 Rh2DG236300
rosa_wichuraiana	Rw1G024190 Rw2G017620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	635
AccII	CGCG	1 cut(s)	242
AciI	CCGC	6 cut(s)	102, 242, 245, 326, 1474, 1517
AclWI	GGATC	3 cut(s)	87, 278, 1498
AcoI	YGGCCR	4 cut(s)	3, 103, 211, 549
AcsI	RAATTY	1 cut(s)	336
AcuI	CTGAAG	1 cut(s)	341
AfaI	GTAC	6 cut(s)	146, 167, 804, 913, 1075, 1087
AfeI	AGCGCT	1 cut(s)	1634
AfiI	CCNNNNNNNGG	4 cut(s)	1394, 1400, 1401, 1516
AflII	CTTAAG	1 cut(s)	371
AjnI	CCWGG	2 cut(s)	288, 1295
AjuI	GAANNNNNNNTTGG	4 cut(s)	1504, 1536, 1683, 1715
Alw21I	GWGCWC	3 cut(s)	123, 381, 1232
Alw26I	GTCTC	2 cut(s)	809, 1377
Alw44I	GTGCAC	1 cut(s)	1228
AlwI	GGATC	3 cut(s)	87, 278, 1498
AlwNI	CAGNNNCTG	2 cut(s)	929, 1256
Ama87I	CYCGRG	1 cut(s)	876
Aor51HI	AGCGCT	1 cut(s)	1634
AoxI	GGCC	6 cut(s)	3, 103, 211, 260, 549, 1433
ApaLI	GTGCAC	1 cut(s)	1228
ApeKI	GCWGC	3 cut(s)	466, 563, 1307
ApoI	RAATTY	1 cut(s)	336
AspLEI	GCGC	1 cut(s)	1635
AspS9I	GGNCC	1 cut(s)	1433
AsuC2I	CCSGG	2 cut(s)	176, 1395
AsuHPI	GGTGA	3 cut(s)	319, 893, 914
AvaI	CYCGRG	1 cut(s)	876
BaeGI	GKGCMC	1 cut(s)	1232
BalI	TGGCCA	2 cut(s)	5, 551
BanI	GGYRCC	1 cut(s)	635
BanII	GRGCYC	2 cut(s)	123, 381
BauI	CACGAG	3 cut(s)	116, 732, 741
BbsI	GAAGAC	3 cut(s)	63, 103, 800
Bbv12I	GWGCWC	3 cut(s)	123, 381, 1232
BbvI	GCAGC	3 cut(s)	478, 575, 1294
BccI	CCATC	4 cut(s)	98, 104, 1076, 1433
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	2 cut(s)	290, 1297
BcnI	CCSGG	2 cut(s)	176, 1395
BcoDI	GTCTC	2 cut(s)	809, 1377
BfaI	CTAG	2 cut(s)	587, 1263
BfmI	CTRYAG	3 cut(s)	470, 1158, 1539
BfoI	RGCGCY	1 cut(s)	1636
BfrI	CTTAAG	1 cut(s)	371
BglII	AGATCT	1 cut(s)	589
BisI	GCNGC	5 cut(s)	103, 243, 467, 564, 1308
BlpI	GCTNAGC	1 cut(s)	1164
BlsI	GCNGC	5 cut(s)	104, 244, 468, 565, 1309
BmcAI	AGTACT	1 cut(s)	146
Bme1390I	CCNGG	4 cut(s)	176, 290, 1297, 1395
BmeT110I	CYCGRG	1 cut(s)	876
BmgT120I	GGNCC	1 cut(s)	1433
BmiI	GGNNCC	1 cut(s)	637
BmrFI	CCNGG	4 cut(s)	176, 290, 1297, 1395
BmsI	GCATC	4 cut(s)	196, 238, 1108, 1237
BoxI	GACNNNNGTC	1 cut(s)	821
BpiI	GAAGAC	3 cut(s)	63, 103, 800
BpmI	CTGGAG	1 cut(s)	779
Bpu1102I	GCTNAGC	1 cut(s)	1164
BpuEI	CTTGAG	1 cut(s)	47
BpuMI	CCSGG	2 cut(s)	176, 1395
BsaBI	GATNNNNATC	2 cut(s)	588, 1495
BsaJI	CCNNGG	4 cut(s)	288, 510, 675, 1672
BsaXI	ACNNNNNCTCC	1 cut(s)	1696
Bsc4I	CCNNNNNNNGG	4 cut(s)	1394, 1400, 1401, 1516
Bse118I	RCCGGY	3 cut(s)	209, 213, 1621
Bse1I	ACTGG	2 cut(s)	579, 762
Bse8I	GATNNNNATC	2 cut(s)	588, 1495
BseBI	CCWGG	2 cut(s)	290, 1297
BseDI	CCNNGG	4 cut(s)	288, 510, 675, 1672
BseGI	GGATG	6 cut(s)	211, 253, 601, 961, 1216, 1411
BseJI	GATNNNNATC	2 cut(s)	588, 1495
BseLI	CCNNNNNNNGG	4 cut(s)	1394, 1400, 1401, 1516
BseMII	CTCAG	3 cut(s)	66, 269, 480
BseNI	ACTGG	2 cut(s)	579, 762
BseRI	GAGGAG	1 cut(s)	401
BseSI	GKGCMC	1 cut(s)	1232
BseX3I	CGGCCG	1 cut(s)	211
BseXI	GCAGC	3 cut(s)	478, 575, 1294
BseYI	CCCAGC	1 cut(s)	331
BsgI	GTGCAG	1 cut(s)	1685
Bsh1236I	CGCG	1 cut(s)	242
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	6 cut(s)	5, 105, 213, 262, 551, 1435
BshNI	GGYRCC	1 cut(s)	635
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	3 cut(s)	123, 381, 1232
BsiHKCI	CYCGRG	1 cut(s)	876
BsiSI	CCGG	5 cut(s)	176, 210, 214, 1395, 1622
BslFI	GGGAC	3 cut(s)	251, 1364, 1654
BslI	CCNNNNNNNGG	4 cut(s)	1394, 1400, 1401, 1516
BsmAI	GTCTC	2 cut(s)	809, 1377
BsmBI	CGTCTC	1 cut(s)	809
BsmFI	GGGAC	3 cut(s)	251, 1364, 1654
BsmI	GAATGC	2 cut(s)	566, 1621
BsnI	GGCC	6 cut(s)	5, 105, 213, 262, 551, 1435
BsoBI	CYCGRG	1 cut(s)	876
Bsp1286I	GDGCHC	3 cut(s)	123, 381, 1232
Bsp143I	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
Bsp1720I	GCTNAGC	1 cut(s)	1164
Bsp19I	CCATGG	1 cut(s)	510
BspACI	CCGC	6 cut(s)	102, 242, 245, 326, 1474, 1517
BspANI	GGCC	6 cut(s)	5, 105, 213, 262, 551, 1435
BspCNI	CTCAG	3 cut(s)	67, 268, 481
BspFNI	CGCG	1 cut(s)	242
BspHI	TCATGA	1 cut(s)	1102
BspLI	GGNNCC	1 cut(s)	637
BspPI	GGATC	3 cut(s)	87, 278, 1498
BspT107I	GGYRCC	1 cut(s)	635
BspTI	CTTAAG	1 cut(s)	371
BsrFI	RCCGGY	3 cut(s)	209, 213, 1621
BsrI	ACTGG	2 cut(s)	579, 762
BssAI	RCCGGY	3 cut(s)	209, 213, 1621
BssECI	CCNNGG	4 cut(s)	288, 510, 675, 1672
BssMI	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
BssSI	CACGAG	3 cut(s)	116, 732, 741
BssT1I	CCWWGG	3 cut(s)	510, 675, 1672
Bst2BI	CACGAG	3 cut(s)	116, 732, 741
Bst2UI	CCWGG	2 cut(s)	290, 1297
Bst4CI	ACNGT	5 cut(s)	135, 820, 1020, 1099, 1600
Bst6I	CTCTTC	1 cut(s)	1549
BstAFI	CTTAAG	1 cut(s)	371
BstC8I	GCNNGC	7 cut(s)	197, 211, 215, 561, 1427, 1472, 1682
BstDEI	CTNAG	6 cut(s)	75, 255, 489, 665, 1164, 1577
BstDSI	CCRYGG	1 cut(s)	510
BstF5I	GGATG	6 cut(s)	211, 253, 601, 961, 1216, 1411
BstFNI	CGCG	1 cut(s)	242
BstH2I	RGCGCY	1 cut(s)	1636
BstHHI	GCGC	1 cut(s)	1635
BstKTI	GATC	8 cut(s)	82, 286, 355, 586, 592, 1345, 1493, 1583
BstMAI	GTCTC	2 cut(s)	809, 1377
BstMBI	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
BstMCI	CGRYCG	1 cut(s)	214
BstMWI	GCNNNNNNNGC	9 cut(s)	48, 127, 180, 248, 376, 472, 737, 860, 1544
BstNI	CCWGG	2 cut(s)	290, 1297
BstNSI	RCATGY	1 cut(s)	466
BstPAI	GACNNNNGTC	1 cut(s)	821
BstSCI	CCNGG	4 cut(s)	174, 288, 1295, 1393
BstSFI	CTRYAG	3 cut(s)	470, 1158, 1539
BstSLI	GKGCMC	1 cut(s)	1232
BstUI	CGCG	1 cut(s)	242
BstV1I	GCAGC	3 cut(s)	478, 575, 1294
BstV2I	GAAGAC	3 cut(s)	63, 103, 800
BstX2I	RGATCY	3 cut(s)	79, 283, 589
BstYI	RGATCY	3 cut(s)	79, 283, 589
BstZI	CGGCCG	1 cut(s)	211
BsuRI	GGCC	6 cut(s)	5, 105, 213, 262, 551, 1435
BtgI	CCRYGG	1 cut(s)	510
BtsCI	GGATG	6 cut(s)	211, 253, 601, 961, 1216, 1411
BtsIMutI	CAGTG	6 cut(s)	140, 930, 1050, 1095, 1583, 1605
Cac8I	GCNNGC	7 cut(s)	197, 211, 215, 561, 1427, 1472, 1682
CaiI	CAGNNNCTG	2 cut(s)	929, 1256
CciI	TCATGA	1 cut(s)	1102
CfoI	GCGC	1 cut(s)	1635
Cfr10I	RCCGGY	3 cut(s)	209, 213, 1621
Cfr13I	GGNCC	1 cut(s)	1433
CseI	GACGC	2 cut(s)	248, 287
Csp6I	GTAC	6 cut(s)	145, 166, 803, 912, 1074, 1086
CviAII	CATG	6 cut(s)	107, 463, 511, 541, 1103, 1351
CviQI	GTAC	6 cut(s)	145, 166, 803, 912, 1074, 1086
DdeI	CTNAG	6 cut(s)	75, 255, 489, 665, 1164, 1577
DpnI	GATC	8 cut(s)	81, 285, 354, 585, 591, 1344, 1492, 1582
DpnII	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
DraI	TTTAAA	1 cut(s)	858
EaeI	YGGCCR	4 cut(s)	3, 103, 211, 549
EagI	CGGCCG	1 cut(s)	211
Eam1104I	CTCTTC	1 cut(s)	1549
EarI	CTCTTC	1 cut(s)	1549
EciI	GGCGGA	2 cut(s)	260, 341
Ecl136II	GAGCTC	2 cut(s)	121, 379
EclXI	CGGCCG	1 cut(s)	211
Eco130I	CCWWGG	3 cut(s)	510, 675, 1672
Eco24I	GRGCYC	2 cut(s)	123, 381
Eco47III	AGCGCT	1 cut(s)	1634
Eco52I	CGGCCG	1 cut(s)	211
Eco53kI	GAGCTC	2 cut(s)	121, 379
Eco57I	CTGAAG	1 cut(s)	341
Eco88I	CYCGRG	1 cut(s)	876
EcoICRI	GAGCTC	2 cut(s)	121, 379
EcoRI	GAATTC	1 cut(s)	336
EcoRII	CCWGG	2 cut(s)	288, 1295
EcoT14I	CCWWGG	3 cut(s)	510, 675, 1672
EcoT38I	GRGCYC	2 cut(s)	123, 381
ErhI	CCWWGG	3 cut(s)	510, 675, 1672
Esp3I	CGTCTC	1 cut(s)	809
FaeI	CATG	6 cut(s)	110, 466, 514, 544, 1106, 1354
FalI	AAGNNNNNCTT	2 cut(s)	375, 407
FaqI	GGGAC	3 cut(s)	251, 1364, 1654
FatI	CATG	6 cut(s)	106, 462, 510, 540, 1102, 1350
FauI	CCCGC	1 cut(s)	1467
Fnu4HI	GCNGC	5 cut(s)	103, 243, 467, 564, 1308
FokI	GGATG	6 cut(s)	218, 260, 608, 948, 1223, 1418
FriOI	GRGCYC	2 cut(s)	123, 381
Fsp4HI	GCNGC	5 cut(s)	103, 243, 467, 564, 1308
FspBI	CTAG	2 cut(s)	587, 1263
GlaI	GCGC	1 cut(s)	1634
GluI	GCNGC	5 cut(s)	103, 243, 467, 564, 1308
GsaI	CCCAGC	1 cut(s)	335
GsuI	CTGGAG	1 cut(s)	779
HaeII	RGCGCY	1 cut(s)	1636
HaeIII	GGCC	6 cut(s)	5, 105, 213, 262, 551, 1435
HapII	CCGG	5 cut(s)	176, 210, 214, 1395, 1622
HgaI	GACGC	2 cut(s)	248, 287
HhaI	GCGC	1 cut(s)	1635
Hin1II	CATG	6 cut(s)	110, 466, 514, 544, 1106, 1354
Hin6I	GCGC	1 cut(s)	1633
HinP1I	GCGC	1 cut(s)	1633
HincII	GTYRAC	2 cut(s)	934, 1219
HindII	GTYRAC	2 cut(s)	934, 1219
HindIII	AAGCTT	2 cut(s)	614, 861
HinfI	GANTC	9 cut(s)	14, 253, 1300, 1321, 1370, 1496, 1520, 1566, 1588
HpaI	GTTAAC	1 cut(s)	1219
HpaII	CCGG	5 cut(s)	176, 210, 214, 1395, 1622
HphI	GGTGA	3 cut(s)	319, 893, 914
Hpy166II	GTNNAC	5 cut(s)	168, 934, 1172, 1219, 1230
Hpy188I	TCNGA	8 cut(s)	76, 258, 583, 946, 1246, 1258, 1482, 1495
Hpy188III	TCNNGA	9 cut(s)	302, 430, 497, 587, 593, 743, 1103, 1325, 1500
Hpy8I	GTNNAC	5 cut(s)	168, 934, 1172, 1219, 1230
Hpy99I	CGWCG	1 cut(s)	303
HpyAV	CCTTC	2 cut(s)	358, 878
HpyCH4III	ACNGT	5 cut(s)	135, 820, 1020, 1099, 1600
HpyF10VI	GCNNNNNNNGC	9 cut(s)	48, 127, 180, 248, 376, 472, 737, 860, 1544
HpyF3I	CTNAG	6 cut(s)	75, 255, 489, 665, 1164, 1577
Hsp92II	CATG	6 cut(s)	110, 466, 514, 544, 1106, 1354
HspAI	GCGC	1 cut(s)	1633
KroI	GCCGGC	2 cut(s)	209, 213
KroNI	GCCGGC	2 cut(s)	211, 215
KspAI	GTTAAC	1 cut(s)	1219
Kzo9I	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
LmnI	GCTCC	7 cut(s)	328, 367, 376, 388, 514, 760, 1544
Lsp1109I	GCAGC	3 cut(s)	478, 575, 1294
LweI	GCATC	4 cut(s)	196, 238, 1108, 1237
MaeI	CTAG	2 cut(s)	587, 1263
MaeIII	GTNAC	1 cut(s)	1283
MalI	GATC	8 cut(s)	81, 285, 354, 585, 591, 1344, 1492, 1582
MboI	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
MboII	GAAGA	9 cut(s)	68, 103, 172, 293, 347, 611, 805, 1566, 1575
MflI	RGATCY	3 cut(s)	79, 283, 589
MhlI	GDGCHC	3 cut(s)	123, 381, 1232
MlsI	TGGCCA	2 cut(s)	5, 551
MluNI	TGGCCA	2 cut(s)	5, 551
MlyI	GAGTC	3 cut(s)	247, 1294, 1379
MmeI	TCCRAC	2 cut(s)	297, 966
Mox20I	TGGCCA	2 cut(s)	5, 551
MroNI	GCCGGC	2 cut(s)	209, 213
MscI	TGGCCA	2 cut(s)	5, 551
MseI	TTAA	6 cut(s)	372, 783, 857, 1067, 1218, 1356
MslI	CAYNNNNRTG	1 cut(s)	966
Msp20I	TGGCCA	2 cut(s)	5, 551
MspCI	CTTAAG	1 cut(s)	371
MspI	CCGG	5 cut(s)	176, 210, 214, 1395, 1622
MspR9I	CCNGG	4 cut(s)	176, 290, 1297, 1395
Mva1269I	GAATGC	2 cut(s)	566, 1621
MvaI	CCWGG	2 cut(s)	290, 1297
MvnI	CGCG	1 cut(s)	242
MwoI	GCNNNNNNNGC	9 cut(s)	48, 127, 180, 248, 376, 472, 737, 860, 1544
NaeI	GCCGGC	2 cut(s)	211, 215
NciI	CCSGG	2 cut(s)	176, 1395
NcoI	CCATGG	1 cut(s)	510
NdeII	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
NgoMIV	GCCGGC	2 cut(s)	209, 213
NlaIII	CATG	6 cut(s)	110, 466, 514, 544, 1106, 1354
NlaIV	GGNNCC	1 cut(s)	637
NspI	RCATGY	1 cut(s)	466
PagI	TCATGA	1 cut(s)	1102
PcsI	WCGNNNNNNNCGW	1 cut(s)	739
PctI	GAATGC	2 cut(s)	566, 1621
PdiI	GCCGGC	2 cut(s)	211, 215
PfeI	GAWTC	6 cut(s)	14, 1321, 1496, 1520, 1566, 1588
PfoI	TCCNGGA	1 cut(s)	1393
PkrI	GCNGC	5 cut(s)	104, 244, 468, 565, 1309
PleI	GAGTC	3 cut(s)	247, 1294, 1378
PpsI	GAGTC	3 cut(s)	247, 1294, 1378
PshAI	GACNNNNGTC	1 cut(s)	821
Psp124BI	GAGCTC	2 cut(s)	123, 381
Psp6I	CCWGG	2 cut(s)	288, 1295
PspFI	CCCAGC	1 cut(s)	331
PspGI	CCWGG	2 cut(s)	288, 1295
PspN4I	GGNNCC	1 cut(s)	637
PspPI	GGNCC	1 cut(s)	1433
PstNI	CAGNNNCTG	2 cut(s)	929, 1256
PsuI	RGATCY	3 cut(s)	79, 283, 589
RsaI	GTAC	6 cut(s)	146, 167, 804, 913, 1075, 1087
RsaNI	GTAC	6 cut(s)	145, 166, 803, 912, 1074, 1086
RseI	CAYNNNNRTG	1 cut(s)	966
SacI	GAGCTC	2 cut(s)	123, 381
SaqAI	TTAA	6 cut(s)	372, 783, 857, 1067, 1218, 1356
SatI	GCNGC	5 cut(s)	103, 243, 467, 564, 1308
Sau3AI	GATC	8 cut(s)	79, 283, 352, 583, 589, 1342, 1490, 1580
Sau96I	GGNCC	1 cut(s)	1433
ScaI	AGTACT	1 cut(s)	146
SchI	GAGTC	3 cut(s)	247, 1294, 1379
ScrFI	CCNGG	4 cut(s)	176, 290, 1297, 1395
SduI	GDGCHC	3 cut(s)	123, 381, 1232
SfaNI	GCATC	4 cut(s)	196, 238, 1108, 1237
SfcI	CTRYAG	3 cut(s)	470, 1158, 1539
SgrAI	CRCCGGYG	1 cut(s)	1621
SmiMI	CAYNNNNRTG	1 cut(s)	966
SmlI	CTYRAG	2 cut(s)	62, 371
SmoI	CTYRAG	2 cut(s)	62, 371
SsiI	CCGC	6 cut(s)	102, 242, 245, 326, 1474, 1517
SspMI	CTAG	2 cut(s)	587, 1263
SstI	GAGCTC	2 cut(s)	123, 381
StyD4I	CCNGG	4 cut(s)	174, 288, 1295, 1393
StyI	CCWWGG	3 cut(s)	510, 675, 1672
TaaI	ACNGT	5 cut(s)	135, 820, 1020, 1099, 1600
TaqI	TCGA	5 cut(s)	141, 301, 351, 506, 825
TatI	WGTACW	3 cut(s)	144, 165, 1073
TauI	GCSGC	2 cut(s)	105, 245
TfiI	GAWTC	6 cut(s)	14, 1321, 1496, 1520, 1566, 1588
Tru1I	TTAA	6 cut(s)	372, 783, 857, 1067, 1218, 1356
Tru9I	TTAA	6 cut(s)	372, 783, 857, 1067, 1218, 1356
TscAI	CASTG	6 cut(s)	140, 930, 1057, 1102, 1590, 1605
TseI	GCWGC	3 cut(s)	466, 563, 1307
TspDTI	ATGAA	7 cut(s)	453, 497, 612, 806, 855, 930, 1091
TspGWI	ACGGA	2 cut(s)	954, 1566
TspRI	CASTG	6 cut(s)	140, 930, 1057, 1102, 1590, 1605
Vha464I	CTTAAG	1 cut(s)	371
VneI	GTGCAC	1 cut(s)	1228
XapI	RAATTY	1 cut(s)	336
XbaI	TCTAGA	1 cut(s)	586
XceI	RCATGY	1 cut(s)	466
XcmI	CCANNNNNNNNNTGG	1 cut(s)	982
XspI	CTAG	2 cut(s)	587, 1263
ZrmI	AGTACT	1 cut(s)	146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.