Rorug02G0175300

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001668
Physical Location & Seq
Forward (+)
16127888 .. 16130004
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0175300.1

Sequence Viewer

Length: 1212 bp
ATGGTTGACGTCGATCGGAGAATGACCGGCCTCAACCCGGCCCACGTCGCCGGCCTCCGCCGCCTCTCCGCCCGAGCCGCCGCCGCCCCAACAACTCCCTCCAACACCAAACCCTCCCTCCCTGTGCGCAACGGTCTCCTCTCATATTCCTCCCTGGCCGAGAATCTCATCACCCACCTTAAAACCTCCGGTATCCAAGTCCAGCCGGGTCTGTCCGAACCCGAATTCGCCCGCGCCGAGGCCGAGTTCGGCTTCGCATTCCCGCCCGACCTCAAAGCCGTTCTATCCGCCGGCCTTCCGGTGGGCCCAGGTTTCCCAGATTGGCGCGCACCCGGTGCTCGCCTCCACCTCAAGGCCTCGCTGGACCTCCCCATCGCCGCGATTTCGTTCCAAATTGCCCGGAATACCCTCTGGTCCAAGTCGTGGGGTCCGAGACCGTCCGACCCGGAAAAGGCCCTCCGGGTCGCGAGGACCGCCTTGAAAAGAGCGCCGCTTTTGATTCCCATTTTCAACCATTGCTACATTCCTTGCAACCCTTGCCTCGCCGGAAACCCCATCTTCTTCGTCGACGAGAATCGGATCTTCTGCTGCGGGTTGGATCTATCCGACTTCTTCGAGCGCGAGTCGGTTTTCCGGAAATCCGACTCCGACCCGTACATCCTCAAGAAGCAACGATCCGTCAGCGAAAAATCCGCCGGGTCGTCCGCGAATTTTTCTCGCCGGAGCCTCGACACGGGTCTCGGGTCCGGAGCCGCCAGAACCCCGAGATGGGTCGAGTTCTGGAGCGACGCCGCCGTGGACCGTCGCCGGAGAAACTCCTCATCATCCTCGTGTTCCTCGCCGGACAGGCTTTTCGATTTGCCGAGGTCCGAATCCCCGAAATGGGTCGACGACTACATTGATCAGATAGGATCCGCTTTGAAACTGGGCGGGTGGTCCGACTCCGATATATCGGATATCGTATCGGTTTCCGCGTCCGGGTTTTTCGAAGGCGAGATGGTCATGTTGGACAACCAAGCCGTGCTGGACGCTCTGCTGCTCAAAGCGGACCGGTTCTCGGACTCGCTCCGAATGGCCGGGTGGAGCTCCGAGGAAGTATCCGACGCGCTGGGTTTCAATTTTCGCGCGGAGAAGGAGAAGAAACCGGCGAAGAAGCTGTCCCCGGAGCTCGTGGAAAGAATCGGGAAACTGGCCGAGTCGGTTTCCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

403

Amino Acids

44.12

Weight (kDa)

8.84

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
Acc16I TGCGCA 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 423
AccI GTMKAC 2 cut(s) 567, 888
AccIII TCCGGA 2 cut(s) 633, 746
AclWI GGATC 5 cut(s) 587, 606, 669, 906, 919
AcoI YGGCCR 3 cut(s) 156, 1074, 1191
AcsI RAATTY 2 cut(s) 224, 709
AcyI GRCGYC 2 cut(s) 9, 789
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 656
AgeI ACCGGT 1 cut(s) 1050
AgsI TTSAA 4 cut(s) 481, 511, 922, 1117
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 153, 307
AluBI AGCT 3 cut(s) 1086, 1156, 1168
AluI AGCT 3 cut(s) 1086, 1156, 1168
Alw21I GWGCWC 3 cut(s) 340, 1088, 1170
Alw26I GTCTC 3 cut(s) 140, 427, 743
AlwI GGATC 5 cut(s) 587, 606, 669, 906, 919
Ama87I CYCGRG 3 cut(s) 72, 740, 763
Aor13HI TCCGGA 2 cut(s) 633, 746
ApaI GGGCCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 588, 1036
ApoI RAATTY 2 cut(s) 224, 709
ArsI GACNNNNNNTTYG 4 cut(s) 836, 868, 1142, 1174
AsiGI ACCGGT 1 cut(s) 1050
AspLEI GCGC 8 cut(s) 129, 236, 327, 329, 490, 621, 1108, 1127
AsuHPI GGTGA 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 987
AvaI CYCGRG 3 cut(s) 72, 740, 763
AvaII GGWCC 9 cut(s) 364, 414, 428, 471, 744, 799, 867, 936, 1048
BaeGI GKGCMC 1 cut(s) 308
BamHI GGATCC 1 cut(s) 911
BanII GRGCYC 3 cut(s) 308, 1088, 1170
BauI CACGAG 2 cut(s) 829, 1169
Bbv12I GWGCWC 3 cut(s) 340, 1088, 1170
BbvI GCAGC 2 cut(s) 575, 1023
BccI CCATC 4 cut(s) 380, 563, 762, 991
BceAI ACGGC 3 cut(s) 263, 779, 1004
BciT130I CCWGG 2 cut(s) 155, 309
BciVI GTATCC 2 cut(s) 203, 1108
BclI TGATCA 1 cut(s) 901
BcoDI GTCTC 3 cut(s) 140, 427, 743
BfoI RGCGCY 1 cut(s) 491
BfuI GTATCC 2 cut(s) 203, 1108
BglI GCCNNNNNGGC 1 cut(s) 847
Bme18I GGWCC 9 cut(s) 364, 414, 428, 471, 744, 799, 867, 936, 1048
BmeT110I CYCGRG 3 cut(s) 72, 740, 763
BmgBI CACGTC 1 cut(s) 46
BmiI GGNNCC 6 cut(s) 306, 429, 725, 745, 751, 913
BmrI ACTGGG 1 cut(s) 935
BmuI ACTGGG 1 cut(s) 935
BoxI GACNNNNGTC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 987
BpuEI CTTGAG 2 cut(s) 335, 647
BsaHI GRCGYC 2 cut(s) 9, 789
BsaI GGTCTC 3 cut(s) 140, 427, 743
BsaJI CCNNGG 7 cut(s) 153, 237, 307, 795, 863, 1089, 1161
BsaWI WCCGGW 5 cut(s) 188, 298, 633, 746, 1050
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bse118I RCCGGY 5 cut(s) 26, 50, 290, 1050, 1144
Bse1I ACTGG 2 cut(s) 930, 1194
Bse3DI GCAATG 1 cut(s) 514
BseAI TCCGGA 2 cut(s) 633, 746
BseBI CCWGG 2 cut(s) 155, 309
BseDI CCNNGG 7 cut(s) 153, 237, 307, 795, 863, 1089, 1161
BseGI GGATG 2 cut(s) 657, 824
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 2 cut(s) 930, 1194
BsePI GCGCGC 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 128, 808
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 2 cut(s) 575, 1023
BseYI CCCAGC 1 cut(s) 1108
Bsh1285I CGRYCG 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 1050
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 3 cut(s) 340, 1088, 1170
BsiHKCI CYCGRG 3 cut(s) 72, 740, 763
BslFI GGGAC 1 cut(s) 1144
BsmAI GTCTC 3 cut(s) 140, 427, 743
BsmFI GGGAC 1 cut(s) 1144
BsmI GAATGC 1 cut(s) 257
Bso31I GGTCTC 3 cut(s) 140, 427, 743
BsoBI CYCGRG 3 cut(s) 72, 740, 763
Bsp119I TTCGAA 1 cut(s) 987
Bsp120I GGGCCC 1 cut(s) 304
Bsp1286I GDGCHC 4 cut(s) 308, 340, 1088, 1170
Bsp13I TCCGGA 2 cut(s) 633, 746
Bsp143I GATC 6 cut(s) 13, 579, 598, 674, 901, 911
Bsp68I TCGCGA 1 cut(s) 467
BspEI TCCGGA 2 cut(s) 633, 746
BspLI GGNNCC 6 cut(s) 306, 429, 725, 745, 751, 913
BspPI GGATC 5 cut(s) 587, 606, 669, 906, 919
BspT104I TTCGAA 1 cut(s) 987
BspTNI GGTCTC 3 cut(s) 140, 427, 743
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 5 cut(s) 26, 50, 290, 1050, 1144
BsrI ACTGG 2 cut(s) 930, 1194
BssAI RCCGGY 5 cut(s) 26, 50, 290, 1050, 1144
BssECI CCNNGG 7 cut(s) 153, 237, 307, 795, 863, 1089, 1161
BssHII GCGCGC 1 cut(s) 325
BssMI GATC 6 cut(s) 13, 579, 598, 674, 901, 911
BssNI GRCGYC 2 cut(s) 9, 789
BssSI CACGAG 2 cut(s) 829, 1169
Bst2BI CACGAG 2 cut(s) 829, 1169
Bst2UI CCWGG 2 cut(s) 155, 309
Bst4CI ACNGT 3 cut(s) 134, 438, 803
BstACI GRCGYC 2 cut(s) 9, 789
BstAPI GCANNNNNTGC 2 cut(s) 335, 537
BstBI TTCGAA 1 cut(s) 987
BstC8I GCNNGC 5 cut(s) 52, 232, 292, 327, 340
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 2 cut(s) 657, 824
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 8 cut(s) 129, 236, 327, 329, 490, 621, 1108, 1127
BstKTI GATC 6 cut(s) 16, 582, 601, 677, 904, 914
BstMAI GTCTC 3 cut(s) 140, 427, 743
BstMBI GATC 6 cut(s) 13, 579, 598, 674, 901, 911
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 8 cut(s) 47, 60, 77, 83, 335, 473, 537, 847
BstNI CCWGG 2 cut(s) 155, 309
BstPAI GACNNNNGTC 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 575, 1023
BstX2I RGATCY 3 cut(s) 579, 598, 911
BstYI RGATCY 3 cut(s) 579, 598, 911
BsuI GTATCC 2 cut(s) 203, 1108
BtgI CCRYGG 1 cut(s) 795
BtgZI GCGATG 1 cut(s) 358
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 2 cut(s) 657, 824
BtuMI TCGCGA 1 cut(s) 467
Cac8I GCNNGC 5 cut(s) 52, 232, 292, 327, 340
CfoI GCGC 8 cut(s) 129, 236, 327, 329, 490, 621, 1108, 1127
Cfr10I RCCGGY 5 cut(s) 26, 50, 290, 1050, 1144
CpoI CGGWCCG 1 cut(s) 1048
CseI GACGC 4 cut(s) 797, 963, 1037, 1112
Csp6I GTAC 1 cut(s) 655
CspAI ACCGGT 1 cut(s) 1050
CspI CGGWCCG 1 cut(s) 1048
CviAII CATG 1 cut(s) 1003
CviQI GTAC 1 cut(s) 655
DpnI GATC 6 cut(s) 15, 581, 600, 676, 903, 913
DpnII GATC 6 cut(s) 13, 579, 598, 674, 901, 911
DraIII CACNNNGTG 1 cut(s) 335
EaeI YGGCCR 3 cut(s) 156, 1074, 1191
EciI GGCGGA 4 cut(s) 47, 58, 277, 682
Ecl136II GAGCTC 2 cut(s) 1086, 1168
Eco147I AGGCCT 1 cut(s) 356
Eco24I GRGCYC 3 cut(s) 308, 1088, 1170
Eco31I GGTCTC 3 cut(s) 140, 427, 743
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 9 cut(s) 364, 414, 428, 471, 744, 799, 867, 936, 1048
Eco53kI GAGCTC 2 cut(s) 1086, 1168
Eco88I CYCGRG 3 cut(s) 72, 740, 763
EcoICRI GAGCTC 2 cut(s) 1086, 1168
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 153, 307
EcoRV GATATC 1 cut(s) 958
EcoT38I GRGCYC 3 cut(s) 308, 1088, 1170
FaeI CATG 1 cut(s) 1006
FaiI YATR 3 cut(s) 145, 950, 1004
FaqI GGGAC 1 cut(s) 1144
FatI CATG 1 cut(s) 1002
FauI CCCGC 4 cut(s) 239, 270, 584, 923
FbaI TGATCA 1 cut(s) 901
FblI GTMKAC 2 cut(s) 567, 888
FokI GGATG 2 cut(s) 644, 811
FriOI GRGCYC 3 cut(s) 308, 1088, 1170
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 8 cut(s) 128, 235, 326, 328, 489, 620, 1107, 1126
GsaI CCCAGC 1 cut(s) 1112
GsuI CTGGAG 1 cut(s) 802
HaeII RGCGCY 1 cut(s) 491
HgaI GACGC 4 cut(s) 797, 963, 1037, 1112
HhaI GCGC 8 cut(s) 129, 236, 327, 329, 490, 621, 1108, 1127
Hin1I GRCGYC 2 cut(s) 9, 789
Hin1II CATG 1 cut(s) 1006
Hin6I GCGC 8 cut(s) 127, 234, 325, 327, 488, 619, 1106, 1125
HinP1I GCGC 8 cut(s) 127, 234, 325, 327, 488, 619, 1106, 1125
HincII GTYRAC 3 cut(s) 7, 568, 889
HindII GTYRAC 3 cut(s) 7, 568, 889
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 4 cut(s) 7, 568, 799, 889
Hpy188III TCNNGA 6 cut(s) 466, 634, 664, 747, 781, 1183
Hpy8I GTNNAC 4 cut(s) 7, 568, 799, 889
Hpy99I CGWCG 8 cut(s) 14, 50, 569, 572, 791, 807, 893, 1106
HpyAV CCTTC 3 cut(s) 305, 983, 1126
HpyCH4III ACNGT 3 cut(s) 134, 438, 803
HpyCH4IV ACGT 2 cut(s) 9, 45
HpyCH4V TGCA 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 60, 77, 83, 335, 473, 537, 847
HpySE526I ACGT 2 cut(s) 9, 45
Hsp92I GRCGYC 2 cut(s) 9, 789
Hsp92II CATG 1 cut(s) 1006
HspAI GCGC 8 cut(s) 127, 234, 325, 327, 488, 619, 1106, 1125
Kpn2I TCCGGA 2 cut(s) 633, 746
KroI GCCGGC 2 cut(s) 50, 290
KroNI GCCGGC 2 cut(s) 52, 292
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 6 cut(s) 13, 579, 598, 674, 901, 911
LmnI GCTCC 7 cut(s) 723, 749, 783, 1071, 1083, 1091, 1165
Lsp1109I GCAGC 2 cut(s) 575, 1023
MaeII ACGT 2 cut(s) 9, 45
MalI GATC 6 cut(s) 15, 581, 600, 676, 903, 913
MboI GATC 6 cut(s) 13, 579, 598, 674, 901, 911
MboII GAAGA 6 cut(s) 550, 553, 574, 604, 1150, 1162
MflI RGATCY 3 cut(s) 579, 598, 911
MhlI GDGCHC 4 cut(s) 308, 340, 1088, 1170
MluCI AATT 4 cut(s) 224, 393, 709, 1117
MlyI GAGTC 5 cut(s) 632, 638, 935, 1055, 1205
MmeI TCCRAC 9 cut(s) 126, 465, 576, 630, 666, 672, 963, 987, 1125
MroI TCCGGA 2 cut(s) 633, 746
MroNI GCCGGC 2 cut(s) 50, 290
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 829
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 2 cut(s) 155, 309
MwoI GCNNNNNNNGC 8 cut(s) 47, 60, 77, 83, 335, 473, 537, 847
NaeI GCCGGC 2 cut(s) 52, 292
NdeII GATC 6 cut(s) 13, 579, 598, 674, 901, 911
NgoMIV GCCGGC 2 cut(s) 50, 290
NlaIII CATG 1 cut(s) 1006
NlaIV GGNNCC 6 cut(s) 306, 429, 725, 745, 751, 913
NmeAIII GCCGAG 4 cut(s) 184, 262, 268, 888
NruI TCGCGA 1 cut(s) 467
NsbI TGCGCA 1 cut(s) 128
NspV TTCGAA 1 cut(s) 987
PauI GCGCGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 356
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 428, 971
PctI GAATGC 1 cut(s) 257
PdiI GCCGGC 2 cut(s) 52, 292
PfeI GAWTC 5 cut(s) 163, 499, 574, 872, 1179
PflMI CCANNNNNTGG 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 1050
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 5 cut(s) 631, 638, 935, 1055, 1204
PpsI GAGTC 5 cut(s) 631, 638, 935, 1055, 1204
PshAI GACNNNNGTC 1 cut(s) 735
Psp124BI GAGCTC 2 cut(s) 1088, 1170
Psp6I CCWGG 2 cut(s) 153, 307
PspFI CCCAGC 1 cut(s) 1108
PspGI CCWGG 2 cut(s) 153, 307
PspN4I GGNNCC 6 cut(s) 306, 429, 725, 745, 751, 913
PspOMI GGGCCC 1 cut(s) 304
PsuI RGATCY 3 cut(s) 579, 598, 911
PteI GCGCGC 1 cut(s) 325
PvuI CGATCG 1 cut(s) 16
RruI TCGCGA 1 cut(s) 467
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 829
Rsr2I CGGWCCG 1 cut(s) 1048
RsrII CGGWCCG 1 cut(s) 1048
SacI GAGCTC 2 cut(s) 1088, 1170
SalI GTCGAC 2 cut(s) 566, 887
SaqAI TTAA 1 cut(s) 180
Sau3AI GATC 6 cut(s) 13, 579, 598, 674, 901, 911
SchI GAGTC 5 cut(s) 632, 638, 935, 1055, 1205
SduI GDGCHC 4 cut(s) 308, 340, 1088, 1170
SfuI TTCGAA 1 cut(s) 987
SgrDI CGTCGACG 1 cut(s) 566
SinI GGWCC 9 cut(s) 364, 414, 428, 471, 744, 799, 867, 936, 1048
SmiMI CAYNNNNRTG 1 cut(s) 829
SmlI CTYRAG 2 cut(s) 350, 662
SmoI CTYRAG 2 cut(s) 350, 662
Sse9I AATT 4 cut(s) 224, 393, 709, 1117
SseBI AGGCCT 1 cut(s) 356
SstI GAGCTC 2 cut(s) 1088, 1170
StuI AGGCCT 1 cut(s) 356
TaaI ACNGT 3 cut(s) 134, 438, 803
TaiI ACGT 2 cut(s) 12, 48
TaqI TCGA 8 cut(s) 12, 567, 615, 729, 774, 855, 888, 987
TasI AATT 4 cut(s) 224, 393, 709, 1117
TauI GCSGC 8 cut(s) 63, 80, 83, 86, 380, 493, 755, 794
TfiI GAWTC 5 cut(s) 163, 499, 574, 872, 1179
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseI GCWGC 2 cut(s) 588, 1036
TspGWI ACGGA 1 cut(s) 667
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 9 cut(s) 364, 414, 428, 471, 744, 799, 867, 936, 1048
XapI RAATTY 2 cut(s) 224, 709
XmiI GTMKAC 2 cut(s) 567, 888
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.