AT4G01940

nifU-like protein 1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003568
Physical Location & Seq
Reverse (-)
841989 .. 843531
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01940.1

Sequence Viewer

Length: 696 bp
ATGATGGCTTCTCTCGCGACTTCCATAAGTGGAAGCTTCAGAATTCTGGTTAAATCATCTTCGACGAGGAATGGGTTTCCTGTAATCTCTGATCAAAACCCGAGTTTCGTCTTGTTTGCGAATAAACGCAGACATATCTCTAGAACGGCGATTTTTCACCGATCTGCGATAAGTGGTTCGAGTCAAGGAGAAAAGATATCACCTTTAGCATCTGGTGTATCTTCAGGTTTATATTCAGCTCAGACTTTTGATTTGACGCCTCAAAACGTTGATTTGGTGCTTGAGGATGTTAGACCGTTCTTGATCTCTGATGGTGGAAACGTCGACGTTGTCTCCGTTGAAGATGGGGTAGTCTCTCTTAAGCTCCAAGGAGCATGTACCAGCTGTCCAAGTTCTTCAACAACTATGACAATGGGAATAGAGAGGGTACTCAAGGAAAAGTTTGGAGATGCATTGAAAGATATTCGACAAGTTTTTGATGAAGAAGTAAAACAGATAACCGTGGAGGCAGTGAATGCTCATCTAGATATACTGAGGCCAGCTATCAAGAACTATGGCGGAAGTGTTGAAGTTTTATCGGTTGAAGGCGAGGACTGCGTTGTAAAATATGTTGGACCGGAGTCGATTGGAATGGGAATACAGGCAGCGATTAAAGAAAAGTTTAAGGACATATCTAATGTAACCTTTACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

231

Amino Acids

24.77

Weight (kDa)

5.67

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 91 - 150 3.4e-22 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 17
AciI CCGC 1 cut(s) 558
AclI AACGTT 1 cut(s) 267
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 2 cut(s) 22, 207
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 2 cut(s) 379, 429
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 5 cut(s) 341, 399, 457, 569, 584
AluBI AGCT 6 cut(s) 36, 239, 364, 384, 542, 693
AluI AGCT 6 cut(s) 36, 239, 364, 384, 542, 693
Alw26I GTCTC 2 cut(s) 337, 358
Ama87I CYCGRG 1 cut(s) 100
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 235, 267
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 149, 192
AvaI CYCGRG 1 cut(s) 100
AvaII GGWCC 1 cut(s) 614
BbvI GCAGC 1 cut(s) 656
BccI CCATC 2 cut(s) 305, 338
BceAI ACGGC 1 cut(s) 162
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 2 cut(s) 337, 358
BfaI CTAG 3 cut(s) 141, 524, 694
BfrI CTTAAG 1 cut(s) 359
BisI GCNGC 1 cut(s) 645
BlsI GCNGC 1 cut(s) 646
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 614
BmsI GCATC 2 cut(s) 218, 439
BoxI GACNNNNGTC 1 cut(s) 619
BpuEI CTTGAG 2 cut(s) 302, 416
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 367, 501
BsaWI WCCGGW 1 cut(s) 616
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
BseDI CCNNGG 2 cut(s) 367, 501
BseGI GGATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 254, 524
BseXI GCAGC 1 cut(s) 656
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 1 cut(s) 617
BsmAI GTCTC 2 cut(s) 337, 358
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 100
Bsp143I GATC 3 cut(s) 91, 161, 303
Bsp68I TCGCGA 1 cut(s) 17
BspACI CCGC 1 cut(s) 558
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 2 cut(s) 253, 525
BspFNI CGCG 1 cut(s) 17
BspTI CTTAAG 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 367, 501
BssMI GATC 3 cut(s) 91, 161, 303
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 367
Bst4CI ACNGT 2 cut(s) 297, 502
BstACI GRCGYC 1 cut(s) 257
BstAFI CTTAAG 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 240, 533
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 94, 164, 306
BstMAI GTCTC 2 cut(s) 337, 358
BstMBI GATC 3 cut(s) 91, 161, 303
BstMWI GCNNNNNNNGC 3 cut(s) 14, 515, 594
BstNSI RCATGY 1 cut(s) 378
BstPAI GACNNNNGTC 1 cut(s) 619
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 656
BsuRI GGCC 1 cut(s) 538
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 1 cut(s) 516
BtuMI TCGCGA 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 614
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 378, 428
CviAII CATG 1 cut(s) 375
CviJI RGCY 8 cut(s) 8, 36, 239, 364, 384, 538, 542, 693
CviKI_1 RGCY 8 cut(s) 8, 36, 239, 364, 384, 538, 542, 693
CviQI GTAC 2 cut(s) 378, 428
DdeI CTNAG 2 cut(s) 240, 533
DpnI GATC 3 cut(s) 93, 163, 305
DpnII GATC 3 cut(s) 91, 161, 303
EciI GGCGGA 1 cut(s) 573
Eco130I CCWWGG 1 cut(s) 367
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 614
Eco57I CTGAAG 2 cut(s) 22, 207
Eco88I CYCGRG 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 42
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 367
EcoT22I ATGCAT 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 1 cut(s) 378
FaiI YATR 9 cut(s) 26, 135, 232, 376, 407, 530, 555, 609, 671
FatI CATG 1 cut(s) 374
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 645
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 645
FspBI CTAG 3 cut(s) 141, 524, 694
GluI GCNGC 1 cut(s) 645
HaeIII GGCC 1 cut(s) 538
HapII CCGG 1 cut(s) 617
HgaI GACGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 1 cut(s) 378
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 2 cut(s) 181, 620
HpaII CCGG 1 cut(s) 617
HphI GGTGA 2 cut(s) 149, 192
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 4 cut(s) 41, 91, 243, 310
Hpy188III TCNNGA 5 cut(s) 16, 141, 301, 524, 547
Hpy8I GTNNAC 1 cut(s) 325
Hpy99I CGWCG 3 cut(s) 67, 326, 329
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 2 cut(s) 297, 502
HpyCH4IV ACGT 3 cut(s) 267, 321, 327
HpyCH4V TGCA 1 cut(s) 452
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 515, 594
HpyF3I CTNAG 2 cut(s) 240, 533
HpySE526I ACGT 3 cut(s) 267, 321, 327
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 161, 303
LmnI GCTCC 2 cut(s) 369, 371
LpnPI CCDG 8 cut(s) 32, 93, 198, 210, 394, 552, 626, 630
Lsp1109I GCAGC 1 cut(s) 656
LweI GCATC 2 cut(s) 218, 439
MaeI CTAG 3 cut(s) 141, 524, 694
MaeII ACGT 3 cut(s) 267, 321, 327
MaeIII GTNAC 1 cut(s) 679
MalI GATC 3 cut(s) 93, 163, 305
MboI GATC 3 cut(s) 91, 161, 303
MboII GAAGA 5 cut(s) 51, 213, 353, 387, 494
MluCI AATT 1 cut(s) 42
MlyI GAGTC 2 cut(s) 190, 629
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 7 cut(s) 60, 270, 277, 417, 499, 528, 583
Mph1103I ATGCAT 1 cut(s) 454
MseI TTAA 4 cut(s) 51, 360, 651, 663
MspA1I CMGCKG 1 cut(s) 384
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 1 cut(s) 617
Mva1269I GAATGC 1 cut(s) 520
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 14, 515, 594
NdeII GATC 3 cut(s) 91, 161, 303
NlaIII CATG 1 cut(s) 378
NruI TCGCGA 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 1 cut(s) 520
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 1 cut(s) 646
PleI GAGTC 2 cut(s) 189, 628
PpsI GAGTC 2 cut(s) 189, 628
PshAI GACNNNNGTC 1 cut(s) 619
Psp1406I AACGTT 1 cut(s) 267
PspPI GGNCC 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 384
RruI TCGCGA 1 cut(s) 17
RsaI GTAC 2 cut(s) 379, 429
RsaNI GTAC 2 cut(s) 378, 428
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 4 cut(s) 51, 360, 651, 663
SatI GCNGC 1 cut(s) 645
Sau3AI GATC 3 cut(s) 91, 161, 303
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 2 cut(s) 190, 629
SfaNI GCATC 2 cut(s) 218, 439
SgrDI CGTCGACG 1 cut(s) 323
SinI GGWCC 1 cut(s) 614
SmlI CTYRAG 3 cut(s) 281, 359, 431
SmoI CTYRAG 3 cut(s) 281, 359, 431
Sse9I AATT 1 cut(s) 42
SsiI CCGC 1 cut(s) 558
SspMI CTAG 3 cut(s) 141, 524, 694
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 297, 502
TaiI ACGT 3 cut(s) 270, 324, 330
TaqI TCGA 5 cut(s) 62, 179, 324, 466, 623
TasI AATT 1 cut(s) 42
Tru1I TTAA 4 cut(s) 51, 360, 651, 663
Tru9I TTAA 4 cut(s) 51, 360, 651, 663
TscAI CASTG 1 cut(s) 516
TseI GCWGC 1 cut(s) 644
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 1 cut(s) 516
Tth111I GACNNNGTC 1 cut(s) 329
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 614
XapI RAATTY 1 cut(s) 42
XbaI TCTAGA 2 cut(s) 140, 523
XceI RCATGY 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 3 cut(s) 141, 524, 694
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.