Rw0G001690

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00049
OG0003568
Physical Location & Seq
Reverse (-)
38113 .. 39036
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001690.1

Sequence Viewer

Length: 669 bp
ATGGCGTCTCTCACCACCTGCTGGTTGCCCAAAACCGCAATTCCTTCTCCTCAAATCCCAGCGAAATCCCATCAGTCCCCACAGCTTATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAAGAAGGATCTTTCTGGGTTCCGCCATTAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCGTCTTCTTCATCTTCTCCGGGACTCTACTCGGCCAAGCAATTCGAGCTCACAGTTCCCAACGTCGACTTGGTCCTCGAGGACGTCCGCCCCTATCTCATCGCCGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATCGAACGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAGAACAGAGAGACCACAGTTGAGGCAGTGAATGGTCATCTCGACATATTGAGGCCGGCGATCAAGAACTTTGGTGGAAGTGTTGAAGTGTTATCAGTTGAAGGTGAGGATTGCCATGTAAAATATGTGGGGCCCGACTCCATTGGATCAGGAATCAGAGCAGCTATTAAGGAGAAGTTCCCAGATATTGTCAATGTTGTGTTTACAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

222

Amino Acids

23.76

Weight (kDa)

5.68

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 82 - 141 1.1e-23 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
AatII GACGTC 1 cut(s) 264
AbsI CCTCGAGG 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 21
AccI GTMKAC 2 cut(s) 243, 297
AciI CCGC 4 cut(s) 36, 141, 171, 265
AclWI GGATC 3 cut(s) 134, 398, 610
AcoI YGGCCR 1 cut(s) 210
AcyI GRCGYC 3 cut(s) 5, 261, 321
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 3 cut(s) 440, 542, 557
AhdI GACNNNNNGTC 1 cut(s) 304
AluBI AGCT 7 cut(s) 85, 152, 226, 337, 357, 366, 620
AluI AGCT 7 cut(s) 85, 152, 226, 337, 357, 366, 620
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 12, 461
AlwI GGATC 3 cut(s) 134, 398, 610
Ama87I CYCGRG 1 cut(s) 254
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 3 cut(s) 210, 509, 587
ApaI GGGCCC 1 cut(s) 591
ApeKI GCWGC 2 cut(s) 167, 617
ArsI GACNNNNNNTTYG 2 cut(s) 306, 338
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 3 cut(s) 250, 587, 588
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 4, 572
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 591
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 228, 591
BbsI GAAGAC 2 cut(s) 174, 321
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 154, 629
BccI CCATC 2 cut(s) 78, 278
BceAI ACGGC 2 cut(s) 90, 334
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 12, 461
BfaI CTAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 3 cut(s) 168, 171, 618
BlsI GCNGC 3 cut(s) 169, 172, 619
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 1 cut(s) 250
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 3 cut(s) 250, 587, 588
BmiI GGNNCC 4 cut(s) 104, 139, 588, 589
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 412
BpiI GAAGAC 2 cut(s) 174, 321
BplI GAGNNNNNCTC 2 cut(s) 411, 443
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 317, 413
BpuMI CCSGG 1 cut(s) 198
BsaHI GRCGYC 3 cut(s) 5, 261, 321
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse118I RCCGGY 1 cut(s) 511
BseDI CCNNGG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 39, 105
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 2 cut(s) 154, 629
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 3 cut(s) 212, 511, 589
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 197, 512
BslFI GGGAC 2 cut(s) 61, 213
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 2 cut(s) 12, 461
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 61, 213
BsnI GGCC 3 cut(s) 212, 511, 589
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 155
Bsp120I GGGCCC 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 228, 591
Bsp143I GATC 4 cut(s) 126, 390, 516, 602
BspACI CCGC 4 cut(s) 36, 141, 171, 265
BspANI GGCC 3 cut(s) 212, 511, 589
BspLI GGNNCC 4 cut(s) 104, 139, 588, 589
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 134, 398, 610
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 4 cut(s) 126, 390, 516, 602
BssNI GRCGYC 3 cut(s) 5, 261, 321
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 102, 232, 475
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 3 cut(s) 5, 261, 321
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 2 cut(s) 163, 513
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 4 cut(s) 129, 393, 519, 605
BstMAI GTCTC 2 cut(s) 12, 461
BstMBI GATC 4 cut(s) 126, 390, 516, 602
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 363
BstSCI CCNGG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 591
BstV1I GCAGC 2 cut(s) 154, 629
BstV2I GAAGAC 2 cut(s) 174, 321
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 3 cut(s) 212, 511, 589
BtgZI GCGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 163, 513
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 3 cut(s) 250, 587, 588
CseI GACGC 1 cut(s) 310
CviAII CATG 2 cut(s) 379, 572
DpnI GATC 4 cut(s) 128, 392, 518, 604
DpnII GATC 4 cut(s) 126, 390, 516, 602
DriI GACNNNNNGTC 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 450
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 450
EciI GGCGGA 2 cut(s) 130, 254
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 2 cut(s) 228, 591
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 250
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 2 cut(s) 228, 591
ErhI CCWWGG 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 382, 575
FaiI YATR 5 cut(s) 89, 380, 503, 573, 582
FaqI GGGAC 2 cut(s) 61, 213
FatI CATG 2 cut(s) 378, 571
FblI GTMKAC 2 cut(s) 243, 297
Fnu4HI GCNGC 3 cut(s) 168, 171, 618
FriOI GRGCYC 2 cut(s) 228, 591
Fsp4HI GCNGC 3 cut(s) 168, 171, 618
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 166
GluI GCNGC 3 cut(s) 168, 171, 618
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 3 cut(s) 212, 511, 589
HapII CCGG 2 cut(s) 197, 512
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 3 cut(s) 5, 261, 321
Hin1II CATG 2 cut(s) 382, 575
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 2 cut(s) 244, 298
HindII GTYRAC 2 cut(s) 244, 298
HinfI GANTC 4 cut(s) 176, 201, 593, 609
HpaII CCGG 2 cut(s) 197, 512
HphI GGTGA 2 cut(s) 4, 572
Hpy166II GTNNAC 3 cut(s) 244, 298, 660
Hpy188I TCNGA 1 cut(s) 614
Hpy188III TCNNGA 3 cut(s) 497, 520, 606
Hpy8I GTNNAC 3 cut(s) 244, 298, 660
Hpy99I CGWCG 5 cut(s) 245, 299, 302, 314, 326
HpyAV CCTTC 3 cut(s) 54, 117, 551
HpyCH4III ACNGT 3 cut(s) 102, 232, 475
HpyCH4IV ACGT 4 cut(s) 240, 261, 294, 300
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 363
HpySE526I ACGT 4 cut(s) 240, 261, 294, 300
Hsp92I GRCGYC 3 cut(s) 5, 261, 321
Hsp92II CATG 2 cut(s) 382, 575
HspAI GCGC 1 cut(s) 165
KroI GCCGGC 1 cut(s) 511
KroNI GCCGGC 1 cut(s) 513
Kzo9I GATC 4 cut(s) 126, 390, 516, 602
LmnI GCTCC 1 cut(s) 342
LpnPI CCDG 9 cut(s) 7, 31, 72, 119, 210, 525, 591, 648, 651
Lsp1109I GCAGC 2 cut(s) 154, 629
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 363
MaeII ACGT 4 cut(s) 240, 261, 294, 300
MalI GATC 4 cut(s) 128, 392, 518, 604
MboI GATC 4 cut(s) 126, 390, 516, 602
MboII GAAGA 5 cut(s) 174, 177, 183, 326, 467
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 228, 591
MluCI AATT 2 cut(s) 39, 218
MlyI GAGTC 3 cut(s) 185, 195, 587
MnlI CCTC 7 cut(s) 60, 126, 250, 263, 472, 501, 556
MroNI GCCGGC 1 cut(s) 511
MseI TTAA 3 cut(s) 110, 405, 624
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 2 cut(s) 197, 512
MspR9I CCNGG 1 cut(s) 198
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 363
NaeI GCCGGC 1 cut(s) 513
NciI CCSGG 1 cut(s) 198
NdeII GATC 4 cut(s) 126, 390, 516, 602
NgoMIV GCCGGC 1 cut(s) 511
NlaIII CATG 2 cut(s) 382, 575
NlaIV GGNNCC 4 cut(s) 104, 139, 588, 589
NmeAIII GCCGAG 2 cut(s) 188, 199
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 318
PdiI GCCGGC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 609
PflFI GACNNNGTC 2 cut(s) 248, 320
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 3 cut(s) 169, 172, 619
PleI GAGTC 3 cut(s) 184, 195, 587
PpsI GAGTC 3 cut(s) 184, 195, 587
Psp124BI GAGCTC 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 4 cut(s) 104, 139, 588, 589
PspOMI GGGCCC 1 cut(s) 587
PspPI GGNCC 3 cut(s) 250, 587, 588
PspXI VCTCGAGB 1 cut(s) 254
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 2 cut(s) 248, 320
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 2 cut(s) 242, 296
SaqAI TTAA 3 cut(s) 110, 405, 624
SatI GCNGC 3 cut(s) 168, 171, 618
Sau3AI GATC 4 cut(s) 126, 390, 516, 602
Sau96I GGNCC 3 cut(s) 250, 587, 588
SchI GAGTC 3 cut(s) 185, 195, 587
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 2 cut(s) 228, 591
SfaNI GCATC 1 cut(s) 412
Sfr274I CTCGAG 1 cut(s) 254
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 296
SinI GGWCC 1 cut(s) 250
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 3 cut(s) 254, 332, 428
SmoI CTYRAG 3 cut(s) 254, 332, 428
Sse9I AATT 2 cut(s) 39, 218
SsiI CCGC 4 cut(s) 36, 141, 171, 265
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 3 cut(s) 102, 232, 475
TaiI ACGT 4 cut(s) 243, 264, 297, 303
TaqI TCGA 8 cut(s) 155, 222, 243, 255, 297, 312, 393, 498
TasI AATT 2 cut(s) 39, 218
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 609
Tru1I TTAA 3 cut(s) 110, 405, 624
Tru9I TTAA 3 cut(s) 110, 405, 624
TscAI CASTG 1 cut(s) 489
TseI GCWGC 2 cut(s) 167, 617
TspDTI ATGAA 3 cut(s) 177, 395, 468
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 489
Tth111I GACNNNGTC 2 cut(s) 248, 320
Van91I CCANNNNNTGG 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 3 cut(s) 244, 347, 382
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 2 cut(s) 243, 297
XspI CTAG 1 cut(s) 363
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.