RLG00000023039

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003568
Physical Location & Seq
Forward (+)
17103188 .. 17104106
919 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023039

Sequence Viewer

Length: 663 bp
ATGGCGTCTCTCACAACCTCCTGGTTGCCCAAAACCCCAACTCCTCAAATCCCAGCGAAATCCCATCAGTCCTCACAACTTATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAAGAAGGATCTTTCTGGGTTCCGCCGTTGGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCTTCTTCTTCTTCTTCTCCGGGACTCTACTCGGCCAAGCAATTCGAGCTCACAGTTCCCAACGTCGACTTGGTTCTCGAGGACGTCCGCCCCTATCTCATCGCCGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGAATCGAACGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAATCTTTGATGAAGAGAACAGAGAGACCACAGTTGAGGCAGTGAATGGTCATCTCGACATATTGAGGCCGGCGATCAAGAACTTTGGTGGAAGTGTTGAAGTGTTATCAGTTGAAGGTGGGGATTGCCATGTAAAATATGTGGGGCCTGACTCCATTGGATCAGGAATCAGAGCAGCTATTAAGGAGAAGTTCCCAGATATTGTCAATGTTGTGTTTACAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

221

Amino Acids

23.39

Weight (kDa)

5.67

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 80 - 139 1.1e-23 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 96
AccI GTMKAC 2 cut(s) 237, 291
AciI CCGC 3 cut(s) 135, 165, 259
AclWI GGATC 2 cut(s) 128, 604
AcoI YGGCCR 1 cut(s) 204
AcyI GRCGYC 3 cut(s) 5, 255, 315
AfeI AGCGCT 1 cut(s) 160
AgsI TTSAA 3 cut(s) 434, 536, 551
AhdI GACNNNNNGTC 1 cut(s) 298
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 6 cut(s) 146, 220, 331, 351, 360, 614
AluI AGCT 6 cut(s) 146, 220, 331, 351, 360, 614
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 12, 455
AlwI GGATC 2 cut(s) 128, 604
Ama87I CYCGRG 1 cut(s) 248
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 3 cut(s) 204, 503, 581
ApeKI GCWGC 2 cut(s) 161, 611
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 1 cut(s) 192
AsuII TTCGAA 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 248
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 222
BbsI GAAGAC 2 cut(s) 165, 315
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 148, 623
BccI CCATC 2 cut(s) 72, 272
BceAI ACGGC 3 cut(s) 84, 122, 328
BciT130I CCWGG 1 cut(s) 22
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 12, 455
BfaI CTAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 3 cut(s) 162, 165, 612
BlsI GCNGC 3 cut(s) 163, 166, 613
Bme1390I CCNGG 2 cut(s) 22, 192
BmeRI GACNNNNNGTC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 98, 133, 582
BmrFI CCNGG 2 cut(s) 22, 192
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 2 cut(s) 165, 315
BplI GAGNNNNNCTC 2 cut(s) 405, 437
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 311, 407
BpuMI CCSGG 1 cut(s) 192
BsaHI GRCGYC 3 cut(s) 5, 255, 315
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bse118I RCCGGY 1 cut(s) 505
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 334
BseRI GAGGAG 2 cut(s) 33, 99
BseXI GCAGC 2 cut(s) 148, 623
BseYI CCCAGC 1 cut(s) 52
BshFI GGCC 3 cut(s) 206, 505, 583
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 191, 506
BslFI GGGAC 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 12, 455
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 207
BsnI GGCC 3 cut(s) 206, 505, 583
Bso31I GGTCTC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 3 cut(s) 120, 510, 596
BspACI CCGC 3 cut(s) 135, 165, 259
BspANI GGCC 3 cut(s) 206, 505, 583
BspLI GGNNCC 3 cut(s) 98, 133, 582
BspPI GGATC 2 cut(s) 128, 604
BspT104I TTCGAA 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 3 cut(s) 120, 510, 596
BssNI GRCGYC 3 cut(s) 5, 255, 315
BssT1I CCWWGG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 96, 226, 469
Bst6I CTCTTC 1 cut(s) 444
BstACI GRCGYC 3 cut(s) 5, 255, 315
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 157, 507
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 123, 513, 599
BstMAI GTCTC 2 cut(s) 12, 455
BstMBI GATC 3 cut(s) 120, 510, 596
BstMWI GCNNNNNNNGC 5 cut(s) 143, 152, 161, 217, 357
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 20, 190
BstV1I GCAGC 2 cut(s) 148, 623
BstV2I GAAGAC 2 cut(s) 165, 315
BstX2I RGATCY 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 3 cut(s) 206, 505, 583
BtgZI GCGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 2 cut(s) 157, 507
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 1 cut(s) 304
CviAII CATG 2 cut(s) 373, 566
DpnI GATC 3 cut(s) 122, 512, 598
DpnII GATC 3 cut(s) 120, 510, 596
DriI GACNNNNNGTC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 444
Eam1105I GACNNNNNGTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 444
EciI GGCGGA 2 cut(s) 124, 248
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 1 cut(s) 455
Eco47III AGCGCT 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 220
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 334
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 376, 569
FaiI YATR 5 cut(s) 83, 374, 497, 567, 576
FaqI GGGAC 1 cut(s) 207
FatI CATG 2 cut(s) 372, 565
FblI GTMKAC 2 cut(s) 237, 291
Fnu4HI GCNGC 3 cut(s) 162, 165, 612
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 3 cut(s) 162, 165, 612
FspBI CTAG 1 cut(s) 357
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 162, 165, 612
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 206, 505, 583
HapII CCGG 2 cut(s) 191, 506
HgaI GACGC 1 cut(s) 304
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 3 cut(s) 5, 255, 315
Hin1II CATG 2 cut(s) 376, 569
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 2 cut(s) 238, 292
HindII GTYRAC 2 cut(s) 238, 292
HinfI GANTC 5 cut(s) 170, 195, 384, 587, 603
HpaII CCGG 2 cut(s) 191, 506
Hpy166II GTNNAC 3 cut(s) 238, 292, 654
Hpy188I TCNGA 1 cut(s) 608
Hpy188III TCNNGA 4 cut(s) 248, 491, 514, 600
Hpy8I GTNNAC 3 cut(s) 238, 292, 654
Hpy99I CGWCG 5 cut(s) 239, 293, 296, 308, 320
HpyAV CCTTC 2 cut(s) 111, 545
HpyCH4III ACNGT 3 cut(s) 96, 226, 469
HpyCH4IV ACGT 4 cut(s) 234, 255, 288, 294
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 152, 161, 217, 357
HpySE526I ACGT 4 cut(s) 234, 255, 288, 294
Hsp92I GRCGYC 3 cut(s) 5, 255, 315
Hsp92II CATG 2 cut(s) 376, 569
HspAI GCGC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 505
KroNI GCCGGC 1 cut(s) 507
Kzo9I GATC 3 cut(s) 120, 510, 596
LmnI GCTCC 2 cut(s) 143, 336
Lsp1109I GCAGC 2 cut(s) 148, 623
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 357
MaeII ACGT 4 cut(s) 234, 255, 288, 294
MalI GATC 3 cut(s) 122, 512, 598
MboI GATC 3 cut(s) 120, 510, 596
MboII GAAGA 7 cut(s) 165, 168, 171, 174, 177, 320, 461
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 1 cut(s) 212
MlyI GAGTC 3 cut(s) 179, 189, 581
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 7 cut(s) 28, 54, 82, 120, 244, 466, 495
MroNI GCCGGC 1 cut(s) 505
MseI TTAA 3 cut(s) 104, 399, 618
MslI CAYNNNNRTG 1 cut(s) 377
MspI CCGG 2 cut(s) 191, 506
MspR9I CCNGG 2 cut(s) 22, 192
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 5 cut(s) 143, 152, 161, 217, 357
NaeI GCCGGC 1 cut(s) 507
NciI CCSGG 1 cut(s) 192
NdeII GATC 3 cut(s) 120, 510, 596
NgoMIV GCCGGC 1 cut(s) 505
NlaIII CATG 2 cut(s) 376, 569
NlaIV GGNNCC 3 cut(s) 98, 133, 582
NmeAIII GCCGAG 2 cut(s) 182, 193
NspV TTCGAA 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 248
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 312
PdiI GCCGGC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 384, 603
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 190
PkrI GCNGC 3 cut(s) 163, 166, 613
PleI GAGTC 3 cut(s) 178, 189, 581
PpsI GAGTC 3 cut(s) 178, 189, 581
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 98, 133, 582
PspPI GGNCC 1 cut(s) 581
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 222
SalI GTCGAC 2 cut(s) 236, 290
SaqAI TTAA 3 cut(s) 104, 399, 618
SatI GCNGC 3 cut(s) 162, 165, 612
Sau3AI GATC 3 cut(s) 120, 510, 596
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 3 cut(s) 179, 189, 581
ScrFI CCNGG 2 cut(s) 22, 192
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 149
SgrDI CGTCGACG 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 248, 326, 422
SmoI CTYRAG 3 cut(s) 248, 326, 422
Sse9I AATT 1 cut(s) 212
SsiI CCGC 3 cut(s) 135, 165, 259
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 20, 190
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 3 cut(s) 96, 226, 469
TaiI ACGT 4 cut(s) 237, 258, 291, 297
TaqI TCGA 8 cut(s) 149, 216, 237, 249, 291, 306, 387, 492
TasI AATT 1 cut(s) 212
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 384, 603
Tru1I TTAA 3 cut(s) 104, 399, 618
Tru9I TTAA 3 cut(s) 104, 399, 618
TscAI CASTG 1 cut(s) 483
TseI GCWGC 2 cut(s) 161, 611
TspDTI ATGAA 2 cut(s) 389, 462
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 1 cut(s) 483
Tth111I GACNNNGTC 1 cut(s) 314
XcmI CCANNNNNNNNNTGG 3 cut(s) 238, 341, 376
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 2 cut(s) 237, 291
XspI CTAG 1 cut(s) 357
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.