Rh5DG137300

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003568
Physical Location & Seq
Forward (+)
13821436 .. 13839648
18213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG137300.1

Sequence Viewer

Length: 624 bp
ATGGTTCATTCTGATTTCTACTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAATGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAACGCAAGCGTTGCCGCTGAGTCTTCTTCTTCTTCTCTAGGACTCTACTTGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCAAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACATTGTGTCCGTCAAAGACGGCGTCATTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTTTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGGGGATGCACTCAAGGATATTCAACAAGTCTTTAATGAAGAAAACAGAGAGACCACAGTTGAGGCTCTTACAAATCAAGCTGGAAAACAGGGGTGGAAGGTTGCAAGTCAAATCATTTGTTTCCTTTCTAAGGCAATTTGTTGTTGGAGCCATGACAATTTCTTTGGTGGTGGACTTGCTCTTATAGGACCTGCTTCACACCACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

207

Amino Acids

22.61

Weight (kDa)

8.52

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 81 - 140 1.8e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 613
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 294
AciI CCGC 2 cut(s) 141, 171
AcoI YGGCCR 1 cut(s) 207
AcyI GRCGYC 2 cut(s) 258, 318
AfiI CCNNNNNNNGG 1 cut(s) 544
AgsI TTSAA 2 cut(s) 392, 437
AhdI GACNNNNNGTC 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 6 cut(s) 152, 223, 334, 354, 363, 494
AluI AGCT 6 cut(s) 152, 223, 334, 354, 363, 494
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 458
AoxI GGCC 2 cut(s) 207, 262
ArsI GACNNNNNNTTYG 4 cut(s) 303, 335, 560, 592
AspS9I GGNCC 2 cut(s) 263, 602
AsuII TTCGAA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 225
BccI CCATC 2 cut(s) 78, 275
BceAI ACGGC 2 cut(s) 90, 331
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 2 cut(s) 194, 360
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 602
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 263, 602
BmiI GGNNCC 3 cut(s) 104, 265, 563
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 3 cut(s) 236, 314, 410
BsaHI GRCGYC 2 cut(s) 258, 318
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 337
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse1I ACTGG 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 2 cut(s) 209, 264
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 2 cut(s) 209, 264
Bso31I GGTCTC 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 225
BspACI CCGC 2 cut(s) 141, 171
BspANI GGCC 2 cut(s) 209, 264
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 104, 265, 563
BspMI ACCTGC 1 cut(s) 613
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 458
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 337
BssNI GRCGYC 2 cut(s) 258, 318
BssT1I CCWWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 472
BstACI GRCGYC 2 cut(s) 258, 318
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 174, 543
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 458
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 382
BsuRI GGCC 2 cut(s) 209, 264
BtsCI GGATG 1 cut(s) 424
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 263, 602
CseI GACGC 1 cut(s) 307
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 2 cut(s) 376, 566
DdeI CTNAG 2 cut(s) 174, 543
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 301
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 2 cut(s) 379, 569
FaiI YATR 5 cut(s) 89, 227, 377, 567, 599
FatI CATG 2 cut(s) 375, 565
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 2 cut(s) 194, 360
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 2 cut(s) 209, 264
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 2 cut(s) 258, 318
Hin1II CATG 2 cut(s) 379, 569
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 3 cut(s) 126, 176, 198
Hpy166II GTNNAC 2 cut(s) 295, 587
Hpy188I TCNGA 1 cut(s) 13
Hpy8I GTNNAC 2 cut(s) 295, 587
Hpy99I CGWCG 2 cut(s) 263, 296
HpyAV CCTTC 2 cut(s) 54, 505
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 2 cut(s) 258, 291
HpyCH4V TGCA 2 cut(s) 422, 518
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
HpyF3I CTNAG 2 cut(s) 174, 543
HpySE526I ACGT 2 cut(s) 258, 291
Hsp92I GRCGYC 2 cut(s) 258, 318
Hsp92II CATG 2 cut(s) 379, 569
LmnI GCTCC 2 cut(s) 339, 561
LpnPI CCDG 5 cut(s) 7, 72, 480, 488, 618
LweI GCATC 1 cut(s) 409
MaeI CTAG 2 cut(s) 194, 360
MaeII ACGT 2 cut(s) 258, 291
MboII GAAGA 7 cut(s) 39, 135, 171, 174, 177, 180, 464
MhlI GDGCHC 1 cut(s) 225
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 4 cut(s) 39, 215, 549, 571
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 185, 192
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 2 cut(s) 126, 469
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 402, 447
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 173
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
NlaIII CATG 2 cut(s) 379, 569
NlaIV GGNNCC 3 cut(s) 104, 265, 563
NspV TTCGAA 1 cut(s) 155
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 2 cut(s) 184, 192
PpsI GAGTC 2 cut(s) 184, 192
PpuMI RGGWCCY 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 602
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 104, 265, 563
PspPI GGNCC 2 cut(s) 263, 602
PspPPI RGGWCCY 1 cut(s) 602
PsyI GACNNNGTC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 402, 447
SatI GCNGC 1 cut(s) 171
Sau96I GGNCC 2 cut(s) 263, 602
SchI GAGTC 2 cut(s) 185, 192
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 1 cut(s) 409
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 251, 329, 425
SmoI CTYRAG 3 cut(s) 251, 329, 425
Sse9I AATT 4 cut(s) 39, 215, 549, 571
SsiI CCGC 2 cut(s) 141, 171
SspMI CTAG 2 cut(s) 194, 360
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 2 cut(s) 261, 294
TaqI TCGA 2 cut(s) 155, 294
TasI AATT 4 cut(s) 39, 215, 549, 571
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 2 cut(s) 402, 447
Tru9I TTAA 2 cut(s) 402, 447
TspDTI ATGAA 2 cut(s) 392, 465
TspGWI ACGGA 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 602
XagI CCTNNNNNAGG 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 3 cut(s) 241, 344, 379
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 2 cut(s) 194, 360
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.