Rh1AG179500

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003568
Physical Location & Seq
Forward (+)
34051715 .. 34063833
12119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG179500.1

Sequence Viewer

Length: 621 bp
ATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCACCATTAGAGCTTCGAACGCAAGCGCTGCCTCCGAGTCTTCTTCTTCTTCTCCGGGACTCTACTCGACCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTTGACGTTGTGTCCGTCGAAGACGGCGTCATTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGGAGATACACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGGCAACATCTGAAACTGGTTCACCTGCGGATGAAACTGAATTTGAGAAGTTAGAAGAGAAAGCCTTCAATTTGAGAAAGACATTCACCATGAGGGCTACTGATGTGACTGGAAAGGTGTCTCAGATAATGATAATGATAAGGAGTCGGACACGGTTTAATGAGCAAAGAACAATTTTTGCTTTTATGAAGATGCCAAGACTGTTGGGGCCGGTGTCATCCAGTCCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

206

Amino Acids

22.85

Weight (kDa)

5.73

Isoelectric Point (pI)

57.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 52 - 111 1.5e-21 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 421
AatII GACGTC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 15
AciI CCGC 1 cut(s) 416
AclI AACGTT 1 cut(s) 204
AcsI RAATTY 1 cut(s) 428
AcyI GRCGYC 2 cut(s) 171, 231
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 3 cut(s) 305, 350, 457
AhdI GACNNNNNGTC 1 cut(s) 214
AluBI AGCT 5 cut(s) 65, 136, 247, 267, 276
AluI AGCT 5 cut(s) 65, 136, 247, 267, 276
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 371, 513
Ama87I CYCGRG 1 cut(s) 164
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 2 cut(s) 175, 596
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 1 cut(s) 428
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 176, 596
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 402, 466
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 164
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 84, 231
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 188
BceAI ACGGC 2 cut(s) 3, 244
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 371, 513
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 108
BmeRI GACNNNNNGTC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 176, 596
BmiI GGNNCC 3 cut(s) 17, 178, 597
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 570
BpiI GAAGAC 2 cut(s) 84, 231
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 227, 323
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 171, 231
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 3 cut(s) 409, 502, 609
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 2 cut(s) 424, 605
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 3 cut(s) 409, 502, 609
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 1 cut(s) 67
BshFI GGCC 2 cut(s) 177, 598
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 107, 599
BslFI GGGAC 1 cut(s) 123
BsmAI GTCTC 2 cut(s) 371, 513
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 2 cut(s) 177, 598
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 138
BspACI CCGC 1 cut(s) 416
BspANI GGCC 2 cut(s) 177, 598
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 3 cut(s) 17, 178, 597
BspMI ACCTGC 1 cut(s) 421
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 3 cut(s) 409, 502, 609
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 1 cut(s) 250
BssNI GRCGYC 2 cut(s) 171, 231
BssT1I CCWWGG 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 15, 385, 543, 591
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 171, 231
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 510
BstF5I GGATG 2 cut(s) 424, 605
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstMAI GTCTC 2 cut(s) 371, 513
BstMWI GCNNNNNNNGC 4 cut(s) 71, 80, 133, 273
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 2 cut(s) 84, 231
BstXI CCANNNNNNTGG 1 cut(s) 295
BsuRI GGCC 2 cut(s) 177, 598
BtsCI GGATG 2 cut(s) 424, 605
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 176, 596
CseI GACGC 1 cut(s) 220
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 2 cut(s) 289, 478
DdeI CTNAG 1 cut(s) 510
DriI GACNNNNNGTC 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 438
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 292, 481
FaiI YATR 4 cut(s) 140, 290, 479, 575
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 288, 477
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 2 cut(s) 431, 592
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 1 cut(s) 273
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 177, 598
HapII CCGG 2 cut(s) 107, 599
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 80
Hin1I GRCGYC 2 cut(s) 171, 231
Hin1II CATG 2 cut(s) 292, 481
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 4 cut(s) 39, 89, 111, 532
HpaII CCGG 2 cut(s) 107, 599
HphI GGTGA 2 cut(s) 402, 466
Hpy166II GTNNAC 2 cut(s) 208, 410
Hpy188I TCNGA 4 cut(s) 88, 400, 513, 537
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 208, 410
Hpy99I CGWCG 2 cut(s) 176, 224
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 4 cut(s) 15, 385, 543, 591
HpyCH4IV ACGT 3 cut(s) 171, 204, 210
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 80, 133, 273
HpyF3I CTNAG 1 cut(s) 510
HpySE526I ACGT 3 cut(s) 171, 204, 210
Hsp92I GRCGYC 2 cut(s) 171, 231
Hsp92II CATG 2 cut(s) 292, 481
HspAI GCGC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 5 cut(s) 120, 390, 426, 483, 612
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 570
MaeI CTAG 1 cut(s) 273
MaeII ACGT 3 cut(s) 171, 204, 210
MaeIII GTNAC 1 cut(s) 493
MboII GAAGA 9 cut(s) 48, 84, 87, 90, 93, 236, 377, 455, 589
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 4 cut(s) 128, 428, 457, 561
MlyI GAGTC 3 cut(s) 98, 105, 541
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 5 cut(s) 39, 94, 160, 382, 474
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 3 cut(s) 23, 315, 546
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 107, 599
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 4 cut(s) 71, 80, 133, 273
NciI CCSGG 1 cut(s) 108
NlaIII CATG 2 cut(s) 292, 481
NlaIV GGNNCC 3 cut(s) 17, 178, 597
NmuCI GTSAC 1 cut(s) 493
NspV TTCGAA 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 421
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 228
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 3 cut(s) 97, 105, 540
PpsI GAGTC 3 cut(s) 97, 105, 540
Psp124BI GAGCTC 1 cut(s) 138
Psp1406I AACGTT 1 cut(s) 204
PspN4I GGNNCC 3 cut(s) 17, 178, 597
PspPI GGNCC 2 cut(s) 176, 596
PsyI GACNNNGTC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 23, 315, 546
SatI GCNGC 1 cut(s) 81
Sau96I GGNCC 2 cut(s) 176, 596
SchI GAGTC 3 cut(s) 98, 105, 541
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 1 cut(s) 570
Sfr274I CTCGAG 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 3 cut(s) 164, 242, 338
SmoI CTYRAG 3 cut(s) 164, 242, 338
Sse9I AATT 4 cut(s) 128, 428, 457, 561
SsiI CCGC 1 cut(s) 416
SspMI CTAG 1 cut(s) 273
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 4 cut(s) 15, 385, 543, 591
TaiI ACGT 3 cut(s) 174, 207, 213
TaqI TCGA 4 cut(s) 68, 119, 165, 222
TasI AATT 4 cut(s) 128, 428, 457, 561
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 3 cut(s) 23, 315, 546
Tru9I TTAA 3 cut(s) 23, 315, 546
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 4 cut(s) 305, 378, 435, 590
TspGWI ACGGA 2 cut(s) 208, 603
Tth111I GACNNNGTC 1 cut(s) 230
XapI RAATTY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 3 cut(s) 154, 257, 292
XhoI CTCGAG 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 1 cut(s) 273
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.