RchiOBHm_Chr4g0387891

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003568
Physical Location & Seq
Forward (+)
3044443 .. 3045183
741 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36116

Sequence Viewer

Length: 495 bp
ATGGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAATGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCTTCTTCTTCTTCTCTGAGAGTCTACTCGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCGTTTCTCTGAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAAGGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCATAGTTGAGGCAGTCTCGACACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

164

Amino Acids

17.78

Weight (kDa)

5.17

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 81 - 140 8.5e-22 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 2 cut(s) 201, 294
AciI CCGC 2 cut(s) 141, 171
AcoI YGGCCR 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 2 cut(s) 258, 318
AfeI AGCGCT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 392, 437
AhdI GACNNNNNGTC 1 cut(s) 301
AjnI CCWGG 1 cut(s) 20
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 5 cut(s) 152, 223, 334, 354, 363
AluI AGCT 5 cut(s) 152, 223, 334, 354, 363
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 458, 487
Ama87I CYCGRG 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 2 cut(s) 207, 262
ApeKI GCWGC 1 cut(s) 167
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 251
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 171, 318
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 78, 275
BceAI ACGGC 2 cut(s) 90, 331
BciT130I CCWGG 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 458, 487
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 2 cut(s) 168, 171
BlsI GCNGC 2 cut(s) 169, 172
Bme1390I CCNGG 1 cut(s) 22
BmeRI GACNNNNNGTC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 3 cut(s) 6, 104, 265
BmrFI CCNGG 1 cut(s) 22
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 2 cut(s) 171, 318
BplI GAGNNNNNCTC 3 cut(s) 408, 440, 467
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 410
BsaHI GRCGYC 2 cut(s) 258, 318
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 337
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 185
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 154
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 2 cut(s) 209, 264
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 251
BsmAI GTCTC 2 cut(s) 458, 487
BsnI GGCC 2 cut(s) 209, 264
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 225
BspACI CCGC 2 cut(s) 141, 171
BspANI GGCC 2 cut(s) 209, 264
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 6, 104, 265
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 458
BssECI CCNNGG 1 cut(s) 337
BssNI GRCGYC 2 cut(s) 258, 318
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 22
BstACI GRCGYC 2 cut(s) 258, 318
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstMAI GTCTC 2 cut(s) 458, 487
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 2 cut(s) 171, 318
BstXI CCANNNNNNTGG 1 cut(s) 382
BsuRI GGCC 2 cut(s) 209, 264
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 263
CseI GACGC 1 cut(s) 307
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 1 cut(s) 376
CviJI RGCY 8 cut(s) 152, 209, 223, 264, 334, 344, 354, 363
CviKI_1 RGCY 8 cut(s) 152, 209, 223, 264, 334, 344, 354, 363
DdeI CTNAG 1 cut(s) 194
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 301
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 458
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 1 cut(s) 379
FaiI YATR 4 cut(s) 89, 227, 377, 470
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 1 cut(s) 375
FblI GTMKAC 2 cut(s) 201, 294
Fnu4HI GCNGC 2 cut(s) 168, 171
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 2 cut(s) 168, 171
FspBI CTAG 1 cut(s) 360
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 168, 171
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 2 cut(s) 209, 264
HgaI GACGC 1 cut(s) 307
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 2 cut(s) 258, 318
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 126, 176, 198
Hpy166II GTNNAC 2 cut(s) 202, 295
Hpy188I TCNGA 3 cut(s) 13, 195, 330
Hpy188III TCNNGA 2 cut(s) 251, 484
Hpy8I GTNNAC 2 cut(s) 202, 295
Hpy99I CGWCG 5 cut(s) 263, 296, 299, 311, 323
HpyAV CCTTC 3 cut(s) 18, 54, 386
HpyCH4IV ACGT 3 cut(s) 258, 291, 297
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 3 cut(s) 258, 291, 297
Hsp92I GRCGYC 2 cut(s) 258, 318
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 339
LpnPI CCDG 3 cut(s) 7, 34, 72
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 360
MaeII ACGT 3 cut(s) 258, 291, 297
MboII GAAGA 8 cut(s) 39, 135, 171, 174, 177, 180, 323, 464
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 39, 215
MlyI GAGTC 2 cut(s) 185, 207
MnlI CCTC 3 cut(s) 126, 247, 469
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 22
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 360
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 3 cut(s) 6, 104, 265
NmeAIII GCCGAG 2 cut(s) 185, 199
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 315
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 2 cut(s) 169, 172
PleI GAGTC 2 cut(s) 184, 206
PpsI GAGTC 2 cut(s) 184, 206
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 6, 104, 265
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 168, 171
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 185, 207
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 1 cut(s) 225
SetI ASST 8 cut(s) 154, 225, 261, 294, 300, 336, 356, 365
SfaNI GCATC 1 cut(s) 409
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 251, 425
SmoI CTYRAG 2 cut(s) 251, 425
Sse9I AATT 2 cut(s) 39, 215
SsiI CCGC 2 cut(s) 141, 171
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 337
TaiI ACGT 3 cut(s) 261, 294, 300
TaqI TCGA 5 cut(s) 155, 252, 294, 309, 485
TasI AATT 2 cut(s) 39, 215
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 392, 465
TspGWI ACGGA 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 3 cut(s) 241, 344, 379
XhoI CTCGAG 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 201, 294
XspI CTAG 1 cut(s) 360
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.