Rh3CG312900

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0003568
Physical Location & Seq
Reverse (-)
33096559 .. 33101131
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG312900.1

Sequence Viewer

Length: 678 bp
ATGGCGTCTCTCACCACCTGCTGGTTTCCCAAAACCCCAATTCCTTCTCCTCAAATCCCAGCGAAATCCCATCAGTCCCCACAACTTATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAACAAGGATCTTTCTGGGTTCCGCCATTAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCGTCGTCTTCTTCTTCTTCATCTTCTCCGGGACTCTACTCAGCCAAGCAATTCGAGCTCACAGTTCCCAACGTCGACTTGGTCCTCGAGGACGTCCGCCCCTATCTCATCGCCGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATCGAACGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAGAACAGAGAGACCACAGTTGAGGCAGTGAATGGTCATCTCGACATATTGAGGCCGGCGATCAAGAACTTTGGTGGAAGTGTTGAAGTGTTATCAGTTGAAGGTGAGGATTGCCATGTAAAATATGTGGGGCCTGACTCCATTGGATCAGGAATCAGAGCAGCTATTAAGGAGAAGTTCCCAGATATTGTCAATGTTGTGTTTACAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

225

Amino Acids

24.03

Weight (kDa)

5.46

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 85 - 144 1.1e-23 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
AatII GACGTC 1 cut(s) 273
AbsI CCTCGAGG 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 21
AccI GTMKAC 2 cut(s) 252, 306
AciI CCGC 3 cut(s) 141, 171, 274
AclWI GGATC 3 cut(s) 134, 407, 619
AcyI GRCGYC 3 cut(s) 5, 270, 330
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 3 cut(s) 449, 551, 566
AhdI GACNNNNNGTC 1 cut(s) 313
AluBI AGCT 6 cut(s) 152, 235, 346, 366, 375, 629
AluI AGCT 6 cut(s) 152, 235, 346, 366, 375, 629
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 12, 470
AlwI GGATC 3 cut(s) 134, 407, 619
Ama87I CYCGRG 1 cut(s) 263
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 2 cut(s) 518, 596
ApeKI GCWGC 2 cut(s) 167, 626
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 259, 596
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 4, 581
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 177, 330
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 154, 638
BccI CCATC 2 cut(s) 78, 287
BceAI ACGGC 2 cut(s) 90, 343
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 12, 470
BfaI CTAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 3 cut(s) 168, 171, 627
BlsI GCNGC 3 cut(s) 169, 172, 628
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 259
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 259, 596
BmiI GGNNCC 3 cut(s) 104, 139, 597
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 421
BpiI GAAGAC 2 cut(s) 177, 330
BplI GAGNNNNNCTC 2 cut(s) 420, 452
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 326, 422
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 3 cut(s) 5, 270, 330
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 349
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse118I RCCGGY 1 cut(s) 520
BseDI CCNNGG 1 cut(s) 349
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 231
BseRI GAGGAG 2 cut(s) 39, 105
BseXI GCAGC 2 cut(s) 154, 638
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 2 cut(s) 520, 598
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 2 cut(s) 206, 521
BslFI GGGAC 2 cut(s) 61, 222
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 2 cut(s) 12, 470
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 61, 222
BsnI GGCC 2 cut(s) 520, 598
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 4 cut(s) 126, 399, 525, 611
BspACI CCGC 3 cut(s) 141, 171, 274
BspANI GGCC 2 cut(s) 520, 598
BspCNI CTCAG 1 cut(s) 230
BspLI GGNNCC 3 cut(s) 104, 139, 597
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 134, 407, 619
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 520
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 4 cut(s) 126, 399, 525, 611
BssNI GRCGYC 3 cut(s) 5, 270, 330
BssT1I CCWWGG 1 cut(s) 349
Bst4CI ACNGT 3 cut(s) 102, 241, 484
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 3 cut(s) 5, 270, 330
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 2 cut(s) 163, 522
BstDEI CTNAG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 4 cut(s) 129, 402, 528, 614
BstMAI GTCTC 2 cut(s) 12, 470
BstMBI GATC 4 cut(s) 126, 399, 525, 611
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
BstSCI CCNGG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 154, 638
BstV2I GAAGAC 2 cut(s) 177, 330
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 520, 598
BtgZI GCGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 163, 522
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 259, 596
CseI GACGC 1 cut(s) 319
CviAII CATG 2 cut(s) 388, 581
DdeI CTNAG 1 cut(s) 217
DpnI GATC 4 cut(s) 128, 401, 527, 613
DpnII GATC 4 cut(s) 126, 399, 525, 611
DriI GACNNNNNGTC 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 2 cut(s) 130, 263
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 596
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 391, 584
FaiI YATR 5 cut(s) 89, 389, 512, 582, 591
FaqI GGGAC 2 cut(s) 61, 222
FatI CATG 2 cut(s) 387, 580
FblI GTMKAC 2 cut(s) 252, 306
Fnu4HI GCNGC 3 cut(s) 168, 171, 627
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 168, 171, 627
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 166
GluI GCNGC 3 cut(s) 168, 171, 627
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 2 cut(s) 520, 598
HapII CCGG 2 cut(s) 206, 521
HgaI GACGC 1 cut(s) 319
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 3 cut(s) 5, 270, 330
Hin1II CATG 2 cut(s) 391, 584
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 2 cut(s) 253, 307
HindII GTYRAC 2 cut(s) 253, 307
HinfI GANTC 4 cut(s) 176, 210, 602, 618
HpaII CCGG 2 cut(s) 206, 521
HphI GGTGA 2 cut(s) 4, 581
Hpy166II GTNNAC 3 cut(s) 253, 307, 669
Hpy188I TCNGA 1 cut(s) 623
Hpy188III TCNNGA 3 cut(s) 506, 529, 615
Hpy8I GTNNAC 3 cut(s) 253, 307, 669
Hpy99I CGWCG 6 cut(s) 184, 254, 308, 311, 323, 335
HpyAV CCTTC 2 cut(s) 54, 560
HpyCH4III ACNGT 3 cut(s) 102, 241, 484
HpyCH4IV ACGT 4 cut(s) 249, 270, 303, 309
HpyCH4V TGCA 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
HpyF3I CTNAG 1 cut(s) 217
HpySE526I ACGT 4 cut(s) 249, 270, 303, 309
Hsp92I GRCGYC 3 cut(s) 5, 270, 330
Hsp92II CATG 2 cut(s) 391, 584
HspAI GCGC 1 cut(s) 165
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
Kzo9I GATC 4 cut(s) 126, 399, 525, 611
LmnI GCTCC 1 cut(s) 351
Lsp1109I GCAGC 2 cut(s) 154, 638
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 372
MaeII ACGT 4 cut(s) 249, 270, 303, 309
MalI GATC 4 cut(s) 128, 401, 527, 613
MboI GATC 4 cut(s) 126, 399, 525, 611
MboII GAAGA 7 cut(s) 177, 180, 183, 186, 192, 335, 476
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 2 cut(s) 39, 227
MlyI GAGTC 3 cut(s) 185, 204, 596
MnlI CCTC 7 cut(s) 60, 126, 259, 272, 481, 510, 565
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 3 cut(s) 110, 414, 633
MslI CAYNNNNRTG 1 cut(s) 392
MspI CCGG 2 cut(s) 206, 521
MspR9I CCNGG 1 cut(s) 207
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
NaeI GCCGGC 1 cut(s) 522
NciI CCSGG 1 cut(s) 207
NdeII GATC 4 cut(s) 126, 399, 525, 611
NgoMIV GCCGGC 1 cut(s) 520
NlaIII CATG 2 cut(s) 391, 584
NlaIV GGNNCC 3 cut(s) 104, 139, 597
NmeAIII GCCGAG 1 cut(s) 199
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 327
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 618
PflFI GACNNNGTC 2 cut(s) 257, 329
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 3 cut(s) 169, 172, 628
PleI GAGTC 3 cut(s) 184, 204, 596
PpsI GAGTC 3 cut(s) 184, 204, 596
Psp124BI GAGCTC 1 cut(s) 237
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 104, 139, 597
PspPI GGNCC 2 cut(s) 259, 596
PspXI VCTCGAGB 1 cut(s) 263
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 2 cut(s) 257, 329
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 2 cut(s) 251, 305
SaqAI TTAA 3 cut(s) 110, 414, 633
SatI GCNGC 3 cut(s) 168, 171, 627
Sau3AI GATC 4 cut(s) 126, 399, 525, 611
Sau96I GGNCC 2 cut(s) 259, 596
SchI GAGTC 3 cut(s) 185, 204, 596
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 1 cut(s) 421
Sfr274I CTCGAG 1 cut(s) 263
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 305
SinI GGWCC 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 3 cut(s) 263, 341, 437
SmoI CTYRAG 3 cut(s) 263, 341, 437
Sse9I AATT 2 cut(s) 39, 227
SsiI CCGC 3 cut(s) 141, 171, 274
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 3 cut(s) 102, 241, 484
TaiI ACGT 4 cut(s) 252, 273, 306, 312
TaqI TCGA 8 cut(s) 155, 231, 252, 264, 306, 321, 402, 507
TasI AATT 2 cut(s) 39, 227
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 618
Tru1I TTAA 3 cut(s) 110, 414, 633
Tru9I TTAA 3 cut(s) 110, 414, 633
TscAI CASTG 1 cut(s) 498
TseI GCWGC 2 cut(s) 167, 626
TspDTI ATGAA 3 cut(s) 186, 404, 477
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 1 cut(s) 498
Tth111I GACNNNGTC 2 cut(s) 257, 329
Van91I CCANNNNNTGG 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 3 cut(s) 253, 356, 391
XhoI CTCGAG 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 252, 306
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.