MD17G1279000.v1.1

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003568
Physical Location & Seq
Forward (+)
33879060 .. 33880907
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1279000.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGCGTCTCTCGCAGCCACCGGCTTCCACAAAGCCCCAACGCTTTCTTCCAAAATCCCACCCAATTCTCACCGATGCCCACCATTTACGTGTTTGAAACGGCACTGTTTCACCGCTAAAACGCCCATCTTTCTGAAAACCGCCATTAGAGCTTCGAACCCAAGCGCCGCCGCCGAGTCCTCTTCTCCGGGACTCTACTCCGCCAAGCAGTACGAACTCACGGTTCCCAACGTCGACTTGGTTCTCGAGGACGTCCGCCCTTATCTCATCTCCGATGGCGGTAACGTCGACGTTGTCTCCGTTGAGGACGGCGTCGTTTCGCTCAAGCTTCAAGGGGCATGTGGGAGCTGTCCGAGCTCAACGACCACCATGAAGATGGGAATCGAAAGGGTGCTCAAGGAAAAGTTTGGAGACGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAATAAAGAGATCACCGTTGAGGTAGTGAATCGTCACCTGGACATATTGAGACCAGCCATAAAGAACTTTGGTGGGAGTGTGGAAGTGTTATCGGTTGAAGGTGGGGATTGCCATGTAAATTATGTAGGGCCTGAGTCCATCGGATCAGGAATCAAAGCAGCGATTAAGGAGAAGTTCCCAGATATCGTCAATGTTGTATTCACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

220

Amino Acids

23.43

Weight (kDa)

6.21

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 79 - 138 1e-23 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccI GTMKAC 2 cut(s) 234, 288
AciI CCGC 7 cut(s) 114, 141, 168, 171, 201, 256, 279
AclWI GGATC 1 cut(s) 601
AcyI GRCGYC 3 cut(s) 5, 252, 312
AfaI GTAC 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 4 cut(s) 97, 332, 431, 548
AjnI CCWGG 1 cut(s) 486
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 4 cut(s) 152, 328, 348, 357
AluI AGCT 4 cut(s) 152, 328, 348, 357
Alw21I GWGCWC 2 cut(s) 359, 396
Alw26I GTCTC 4 cut(s) 12, 301, 405, 493
AlwI GGATC 1 cut(s) 601
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 2 cut(s) 14, 608
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 578
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 4 cut(s) 62, 103, 454, 476
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 245
BanII GRGCYC 1 cut(s) 359
Bbv12I GWGCWC 2 cut(s) 359, 396
BbvI GCAGC 2 cut(s) 26, 620
BccI CCATC 4 cut(s) 134, 269, 370, 596
BceAI ACGGC 2 cut(s) 116, 325
BciT130I CCWGG 1 cut(s) 488
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 4 cut(s) 12, 301, 405, 493
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 4 cut(s) 15, 168, 171, 609
BlsI GCNGC 4 cut(s) 16, 169, 172, 610
Bme1390I CCNGG 2 cut(s) 189, 488
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 2 cut(s) 189, 488
BmsI GCATC 1 cut(s) 65
BplI GAGNNNNNCTC 2 cut(s) 402, 434
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 3 cut(s) 308, 380, 404
BpuMI CCSGG 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 90
BsaBI GATNNNNATC 1 cut(s) 380
BsaHI GRCGYC 3 cut(s) 5, 252, 312
BsaI GGTCTC 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 658
Bse8I GATNNNNATC 1 cut(s) 380
BseBI CCWGG 1 cut(s) 488
BseJI GATNNNNATC 1 cut(s) 380
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 658
BseXI GCAGC 2 cut(s) 26, 620
BshFI GGCC 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 359, 396
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 21, 188
BslFI GGGAC 1 cut(s) 204
BsmAI GTCTC 4 cut(s) 12, 301, 405, 493
BsmBI CGTCTC 2 cut(s) 12, 405
BsmFI GGGAC 1 cut(s) 204
BsnI GGCC 1 cut(s) 580
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 359, 396
Bsp143I GATC 2 cut(s) 459, 593
BspACI CCGC 7 cut(s) 114, 141, 168, 171, 201, 256, 279
BspANI GGCC 1 cut(s) 580
BspCNI CTCAG 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 1 cut(s) 601
BspT104I TTCGAA 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 1 cut(s) 658
BssAI RCCGGY 1 cut(s) 20
BssMI GATC 2 cut(s) 459, 593
BssNI GRCGYC 3 cut(s) 5, 252, 312
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 3 cut(s) 107, 223, 466
Bst6I CTCTTC 1 cut(s) 187
BstACI GRCGYC 3 cut(s) 5, 252, 312
BstBAI YACGTR 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 462, 596
BstMAI GTCTC 4 cut(s) 12, 301, 405, 493
BstMBI GATC 2 cut(s) 459, 593
BstMWI GCNNNNNNNGC 3 cut(s) 11, 149, 354
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 187, 486
BstV1I GCAGC 2 cut(s) 26, 620
BstXI CCANNNNNNTGG 1 cut(s) 376
BsuRI GGCC 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 103, 651
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 2 cut(s) 301, 422
Csp6I GTAC 1 cut(s) 211
CviAII CATG 3 cut(s) 339, 370, 563
CviJI RGCY 9 cut(s) 17, 24, 35, 152, 328, 348, 357, 506, 580
CviKI_1 RGCY 9 cut(s) 17, 24, 35, 152, 328, 348, 357, 506, 580
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 582
DpnI GATC 2 cut(s) 461, 595
DpnII GATC 2 cut(s) 459, 593
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
EciI GGCGGA 2 cut(s) 190, 245
Ecl136II GAGCTC 1 cut(s) 357
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 493
Eco32I GATATC 1 cut(s) 634
Eco53kI GAGCTC 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 486
EcoRV GATATC 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 359
Esp3I CGTCTC 2 cut(s) 12, 405
FaeI CATG 3 cut(s) 342, 373, 566
FaiI YATR 6 cut(s) 340, 371, 494, 509, 564, 573
FaqI GGGAC 1 cut(s) 204
FatI CATG 3 cut(s) 338, 369, 562
FblI GTMKAC 2 cut(s) 234, 288
Fnu4HI GCNGC 4 cut(s) 15, 168, 171, 609
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 15, 168, 171, 609
GlaI GCGC 1 cut(s) 166
GluI GCNGC 4 cut(s) 15, 168, 171, 609
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 580
HapII CCGG 2 cut(s) 21, 188
HgaI GACGC 2 cut(s) 301, 422
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 3 cut(s) 5, 252, 312
Hin1II CATG 3 cut(s) 342, 373, 566
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 2 cut(s) 235, 289
HindII GTYRAC 2 cut(s) 235, 289
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 6 cut(s) 176, 192, 381, 478, 584, 600
HpaII CCGG 2 cut(s) 21, 188
HphI GGTGA 4 cut(s) 62, 103, 454, 476
Hpy166II GTNNAC 2 cut(s) 235, 289
Hpy188I TCNGA 4 cut(s) 135, 274, 354, 593
Hpy188III TCNNGA 2 cut(s) 245, 597
Hpy8I GTNNAC 2 cut(s) 235, 289
Hpy99I CGWCG 4 cut(s) 236, 290, 293, 317
HpyAV CCTTC 1 cut(s) 542
HpyCH4III ACNGT 3 cut(s) 107, 223, 466
HpyCH4IV ACGT 5 cut(s) 89, 231, 252, 285, 291
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 149, 354
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 5 cut(s) 89, 231, 252, 285, 291
Hsp92I GRCGYC 3 cut(s) 5, 252, 312
Hsp92II CATG 3 cut(s) 342, 373, 566
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 459, 593
LmnI GCTCC 1 cut(s) 345
LpnPI CCDG 9 cut(s) 34, 201, 473, 500, 516, 582, 594, 639, 642
Lsp1109I GCAGC 2 cut(s) 26, 620
LweI GCATC 1 cut(s) 65
MaeII ACGT 5 cut(s) 89, 231, 252, 285, 291
MaeIII GTNAC 2 cut(s) 281, 482
MalI GATC 2 cut(s) 461, 595
MboI GATC 2 cut(s) 459, 593
MboII GAAGA 4 cut(s) 39, 174, 385, 458
MhlI GDGCHC 2 cut(s) 359, 396
MluCI AATT 2 cut(s) 64, 568
MlyI GAGTC 3 cut(s) 185, 186, 593
MnlI CCTC 4 cut(s) 190, 241, 298, 463
MseI TTAA 1 cut(s) 615
MslI CAYNNNNRTG 2 cut(s) 88, 374
MspI CCGG 2 cut(s) 21, 188
MspR9I CCNGG 2 cut(s) 189, 488
MvaI CCWGG 1 cut(s) 488
MwoI GCNNNNNNNGC 3 cut(s) 11, 149, 354
NciI CCSGG 1 cut(s) 189
NdeII GATC 2 cut(s) 459, 593
NlaIII CATG 3 cut(s) 342, 373, 566
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 1 cut(s) 342
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 3 cut(s) 381, 478, 600
PflFI GACNNNGTC 2 cut(s) 293, 311
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 4 cut(s) 16, 169, 172, 610
PleI GAGTC 3 cut(s) 184, 186, 592
PpsI GAGTC 3 cut(s) 184, 186, 592
Ppu21I YACGTR 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 578
PsyI GACNNNGTC 2 cut(s) 293, 311
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 88, 374
SacI GAGCTC 1 cut(s) 359
SalI GTCGAC 2 cut(s) 233, 287
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 4 cut(s) 15, 168, 171, 609
Sau3AI GATC 2 cut(s) 459, 593
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 3 cut(s) 185, 186, 593
ScrFI CCNGG 2 cut(s) 189, 488
SduI GDGCHC 2 cut(s) 359, 396
SfaNI GCATC 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 287
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 88, 374
SmlI CTYRAG 4 cut(s) 245, 323, 395, 419
SmoI CTYRAG 4 cut(s) 245, 323, 395, 419
Sse9I AATT 2 cut(s) 64, 568
SsiI CCGC 7 cut(s) 114, 141, 168, 171, 201, 256, 279
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 187, 486
TaaI ACNGT 3 cut(s) 107, 223, 466
TaiI ACGT 5 cut(s) 92, 234, 255, 288, 294
TaqI TCGA 5 cut(s) 155, 234, 246, 288, 384
TasI AATT 2 cut(s) 64, 568
TauI GCSGC 2 cut(s) 170, 173
TfiI GAWTC 3 cut(s) 381, 478, 600
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 2 cut(s) 110, 658
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 14, 608
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 2 cut(s) 386, 459
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 2 cut(s) 110, 658
Tth111I GACNNNGTC 2 cut(s) 293, 311
XceI RCATGY 1 cut(s) 342
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 373
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 234, 288
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.