Rh2DG406100

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003568
Physical Location & Seq
Reverse (-)
58951183 .. 58951790
608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG406100.1

Sequence Viewer

Length: 444 bp
ATGCTTGTAAAACGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTATTCAGCCATTAGAGCTTCGAACGAAAGCGCTGCCGCCGAGTCTTCTTTTGCTTCTCCGGGACTCTACTCGGCCAAGCAATTTAAGCTCACAGTTCCCAACATTGACTTGGTTCTTGAGGACGTTCGCCCCTGGCTCATCAATGATGGTGGAAATGTCGACATTGTGTCCGTCGAAGACGGTGTCGTTTCTCTGAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGCAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACATAGAGACCACAGTTGAGGTGAGAGCTTTTGATTTCTCGACATGTTTATACAGTGTCGATGGATTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

147

Amino Acids

16.21

Weight (kDa)

4.88

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 52 - 110 1.2e-22 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 207
AciI CCGC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 263
AfeI AGCGCT 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 2 cut(s) 305, 350
AhdI GACNNNNNGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 6 cut(s) 65, 136, 247, 267, 276, 398
AluI AGCT 6 cut(s) 65, 136, 247, 267, 276, 398
Alw26I GTCTC 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AspLEI GCGC 1 cut(s) 80
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 403
AsuII TTCGAA 1 cut(s) 68
BanI GGYRCC 1 cut(s) 15
BbsI GAAGAC 2 cut(s) 84, 231
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 188, 425
BceAI ACGGC 1 cut(s) 3
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 181
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 2 cut(s) 81, 84
BlsI GCNGC 2 cut(s) 82, 85
Bme1390I CCNGG 2 cut(s) 108, 181
BmeRI GACNNNNNGTC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 2 cut(s) 108, 181
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 84, 231
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 185, 323
BpuMI CCSGG 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 179, 250
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 179, 250
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 1 cut(s) 67
BshFI GGCC 1 cut(s) 122
BshNI GGYRCC 1 cut(s) 15
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 1 cut(s) 122
Bso31I GGTCTC 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 68
BspACI CCGC 1 cut(s) 84
BspANI GGCC 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 17
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 179, 250
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 5 cut(s) 15, 142, 230, 385, 425
BstBI TTCGAA 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstMAI GTCTC 1 cut(s) 371
BstMWI GCNNNNNNNGC 3 cut(s) 62, 133, 273
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 106, 179
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 2 cut(s) 84, 231
BstXI CCANNNNNNTGG 1 cut(s) 295
BsuRI GGCC 1 cut(s) 122
BtsIMutI CAGTG 1 cut(s) 430
CfoI GCGC 1 cut(s) 80
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 2 cut(s) 289, 414
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 120
Eam1105I GACNNNNNGTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 292, 417
FaiI YATR 4 cut(s) 290, 374, 415, 421
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 288, 413
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 2 cut(s) 81, 84
Fsp4HI GCNGC 2 cut(s) 81, 84
FspBI CTAG 1 cut(s) 273
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 81, 84
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 122
HapII CCGG 1 cut(s) 107
HhaI GCGC 1 cut(s) 80
Hin1II CATG 2 cut(s) 292, 417
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 3 cut(s) 39, 89, 111
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 3 cut(s) 164, 409, 441
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 224
HpyCH4III ACNGT 5 cut(s) 15, 142, 230, 385, 425
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 329, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 133, 273
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 292, 417
HspAI GCGC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 3 cut(s) 120, 166, 193
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 273
MaeII ACGT 1 cut(s) 171
MboII GAAGA 4 cut(s) 48, 84, 236, 377
MluCI AATT 1 cut(s) 128
MlyI GAGTC 2 cut(s) 98, 105
MnlI CCTC 3 cut(s) 39, 160, 382
MseI TTAA 2 cut(s) 132, 315
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 108, 181
MvaI CCWGG 1 cut(s) 181
MwoI GCNNNNNNNGC 3 cut(s) 62, 133, 273
NciI CCSGG 1 cut(s) 108
NlaIII CATG 2 cut(s) 292, 417
NlaIV GGNNCC 1 cut(s) 17
NmeAIII GCCGAG 2 cut(s) 98, 112
NspI RCATGY 1 cut(s) 417
NspV TTCGAA 1 cut(s) 68
PciI ACATGT 1 cut(s) 413
PfeI GAWTC 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 2 cut(s) 82, 85
PleI GAGTC 2 cut(s) 97, 105
PpsI GAGTC 2 cut(s) 97, 105
PscI ACATGT 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 179
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 293
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 2 cut(s) 132, 315
SatI GCNGC 2 cut(s) 81, 84
SchI GAGTC 2 cut(s) 98, 105
ScrFI CCNGG 2 cut(s) 108, 181
SetI ASST 8 cut(s) 67, 138, 174, 249, 269, 278, 393, 400
SfaNI GCATC 1 cut(s) 322
SfuI TTCGAA 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 164, 338
SmoI CTYRAG 2 cut(s) 164, 338
Sse9I AATT 1 cut(s) 128
SsiI CCGC 1 cut(s) 84
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 106, 179
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 5 cut(s) 15, 142, 230, 385, 425
TaiI ACGT 1 cut(s) 174
TaqI TCGA 5 cut(s) 68, 207, 222, 410, 429
TasI AATT 1 cut(s) 128
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 2 cut(s) 132, 315
Tru9I TTAA 2 cut(s) 132, 315
TscAI CASTG 1 cut(s) 430
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 305, 378
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 1 cut(s) 430
Tth111I GACNNNGTC 1 cut(s) 230
XceI RCATGY 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 3 cut(s) 154, 257, 292
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.