Rw6G010010

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003568
Physical Location & Seq
Forward (+)
17123517 .. 17125858
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010010.1

Sequence Viewer

Length: 612 bp
ATGGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAAGAAGAATCTTTCTCTGTTCTGCCATTAGAGCTTCGAACGCAAGCGGTGCCTCCGAGTCTTCTTCTTCTTCTCCGGGACTCTACTCGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACGTTGTGTTCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGTGGGATTTCATTTCCTTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGCGATTTGGCCGAAAGAGCTCGATTTTGAGCTGTGAAGGTTACTGTAGCAGTTTGGAGTTTTCATCGATTTCCGACGATTCCGGCCGTCTCCGGCCACCAAACCGGTGTCGAAGGTTCGGTTTTTGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

203

Amino Acids

22.38

Weight (kDa)

7.53

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 81 - 141 1.8e-15 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 102, 167
AccI GTMKAC 1 cut(s) 294
AciI CCGC 1 cut(s) 165
AcoI YGGCCR 4 cut(s) 207, 487, 562, 572
AcyI GRCGYC 2 cut(s) 258, 318
AgeI ACCGGT 1 cut(s) 582
AgsI TTSAA 2 cut(s) 395, 440
AjnI CCWGG 1 cut(s) 20
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 6 cut(s) 152, 223, 334, 366, 498, 510
AluI AGCT 6 cut(s) 152, 223, 334, 366, 498, 510
Alw21I GWGCWC 2 cut(s) 225, 500
Alw26I GTCTC 2 cut(s) 461, 572
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 207, 262, 487, 562, 572
ArsI GACNNNNNNTTYG 4 cut(s) 287, 303, 319, 335
AsiGI ACCGGT 1 cut(s) 582
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 195
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 251
BanI GGYRCC 2 cut(s) 102, 167
BanII GRGCYC 2 cut(s) 225, 500
BbsI GAAGAC 2 cut(s) 171, 318
Bbv12I GWGCWC 2 cut(s) 225, 500
BccI CCATC 2 cut(s) 78, 275
BceAI ACGGC 3 cut(s) 90, 331, 549
BciT130I CCWGG 1 cut(s) 22
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 2 cut(s) 461, 572
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 22, 195
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 4 cut(s) 6, 104, 169, 265
BmrFI CCNGG 2 cut(s) 22, 195
BmsI GCATC 1 cut(s) 412
BpiI GAAGAC 2 cut(s) 171, 318
BplI GAGNNNNNCTC 2 cut(s) 411, 443
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 314, 413
BpuMI CCSGG 1 cut(s) 195
Bsa29I ATCGAT 1 cut(s) 545
BsaHI GRCGYC 2 cut(s) 258, 318
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 1 cut(s) 337
BsaWI WCCGGW 1 cut(s) 582
Bse118I RCCGGY 1 cut(s) 582
BseBI CCWGG 1 cut(s) 22
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 105
BseX3I CGGCCG 1 cut(s) 562
BseYI CCCAGC 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 5 cut(s) 209, 264, 489, 564, 574
BshNI GGYRCC 2 cut(s) 102, 167
BshTI ACCGGT 1 cut(s) 582
BshVI ATCGAT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 225, 500
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 4 cut(s) 194, 561, 571, 583
BslFI GGGAC 1 cut(s) 210
BsmAI GTCTC 2 cut(s) 461, 572
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 5 cut(s) 209, 264, 489, 564, 574
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 225, 500
BspACI CCGC 1 cut(s) 165
BspANI GGCC 5 cut(s) 209, 264, 489, 564, 574
BspDI ATCGAT 1 cut(s) 545
BspLI GGNNCC 4 cut(s) 6, 104, 169, 265
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 2 cut(s) 102, 167
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 582
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 1 cut(s) 337
BssNI GRCGYC 2 cut(s) 258, 318
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 102, 475, 524
BstACI GRCGYC 2 cut(s) 258, 318
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstMAI GTCTC 2 cut(s) 461, 572
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 495
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 20, 193
BstSFI CTRYAG 1 cut(s) 523
BstV2I GAAGAC 2 cut(s) 171, 318
BstXI CCANNNNNNTGG 1 cut(s) 385
BstZI CGGCCG 1 cut(s) 562
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 5 cut(s) 209, 264, 489, 564, 574
BsuTUI ATCGAT 1 cut(s) 545
Cac8I GCNNGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 1 cut(s) 307
CspAI ACCGGT 1 cut(s) 582
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 1 cut(s) 379
EaeI YGGCCR 4 cut(s) 207, 487, 562, 572
EagI CGGCCG 1 cut(s) 562
Ecl136II GAGCTC 2 cut(s) 223, 498
EclXI CGGCCG 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 225, 500
Eco31I GGTCTC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 562
Eco53kI GAGCTC 2 cut(s) 223, 498
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 2 cut(s) 223, 498
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 225, 500
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 1 cut(s) 382
FaiI YATR 3 cut(s) 89, 227, 380
FaqI GGGAC 1 cut(s) 210
FatI CATG 1 cut(s) 378
FblI GTMKAC 1 cut(s) 294
FriOI GRGCYC 2 cut(s) 225, 500
FspBI CTAG 1 cut(s) 363
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 5 cut(s) 209, 264, 489, 564, 574
HapII CCGG 4 cut(s) 194, 561, 571, 583
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 2 cut(s) 258, 318
Hin1II CATG 1 cut(s) 382
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 4 cut(s) 126, 176, 198, 557
HpaII CCGG 4 cut(s) 194, 561, 571, 583
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 13, 175, 553
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 6 cut(s) 263, 296, 299, 311, 323, 557
HpyAV CCTTC 4 cut(s) 18, 54, 509, 585
HpyCH4III ACNGT 3 cut(s) 102, 475, 524
HpyCH4IV ACGT 3 cut(s) 258, 291, 297
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 495
HpySE526I ACGT 3 cut(s) 258, 291, 297
Hsp92I GRCGYC 2 cut(s) 258, 318
Hsp92II CATG 1 cut(s) 382
LmnI GCTCC 1 cut(s) 339
LpnPI CCDG 7 cut(s) 7, 34, 72, 207, 574, 584, 596
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 363
MaeII ACGT 3 cut(s) 258, 291, 297
MaeIII GTNAC 1 cut(s) 518
MboII GAAGA 8 cut(s) 39, 135, 171, 174, 177, 180, 323, 467
MhlI GDGCHC 2 cut(s) 225, 500
MluCI AATT 2 cut(s) 39, 215
MlyI GAGTC 2 cut(s) 185, 192
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 3 cut(s) 126, 181, 247
MseI TTAA 2 cut(s) 110, 405
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 4 cut(s) 194, 561, 571, 583
MspR9I CCNGG 2 cut(s) 22, 195
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 495
NciI CCSGG 1 cut(s) 195
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 4 cut(s) 6, 104, 169, 265
NmeAIII GCCGAG 1 cut(s) 185
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 315
PfeI GAWTC 2 cut(s) 126, 557
PflFI GACNNNGTC 1 cut(s) 317
PfoI TCCNGGA 1 cut(s) 193
PinAI ACCGGT 1 cut(s) 582
PleI GAGTC 2 cut(s) 184, 192
PpsI GAGTC 2 cut(s) 184, 192
Psp124BI GAGCTC 2 cut(s) 225, 500
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 4 cut(s) 6, 104, 169, 265
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 2 cut(s) 225, 500
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 110, 405
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 185, 192
ScrFI CCNGG 2 cut(s) 22, 195
SduI GDGCHC 2 cut(s) 225, 500
SfaNI GCATC 1 cut(s) 412
SfcI CTRYAG 1 cut(s) 523
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 3 cut(s) 251, 329, 428
SmoI CTYRAG 3 cut(s) 251, 329, 428
Sse9I AATT 2 cut(s) 39, 215
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 2 cut(s) 225, 500
StyD4I CCNGG 2 cut(s) 20, 193
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 102, 475, 524
TaiI ACGT 3 cut(s) 261, 294, 300
TaqI TCGA 7 cut(s) 155, 252, 294, 309, 500, 545, 589
TasI AATT 2 cut(s) 39, 215
TfiI GAWTC 2 cut(s) 126, 557
Tru1I TTAA 2 cut(s) 110, 405
Tru9I TTAA 2 cut(s) 110, 405
TspDTI ATGAA 4 cut(s) 339, 395, 468, 531
Tth111I GACNNNGTC 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 2 cut(s) 241, 382
XhoI CTCGAG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 1 cut(s) 363
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.