Rw4G018310

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003568
Physical Location & Seq
Forward (+)
40732125 .. 40732912
788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018310.1

Sequence Viewer

Length: 561 bp
ATGCTTGTAAAACGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTTTGTTCCGCCAATAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCTTCTTCTTCTTCTCCGGTACTCTACTCGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACATTGTGTCCGTCATAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGAATTGAAAGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGTTTATGATTGTAAGTGCAGGCAGTCTCGACACATTGAGGCCGGCGAAGAATTTTGAACTATTGATGCTATATGGTAGCATGTTGCCAATTGCATTACGTGCTGTTTTTCTAACTCAAATAGGTACTGGAACCTTTTTGTTCCAAAAGTTCAGTATTTTTACTATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

186

Amino Acids

20.32

Weight (kDa)

5.54

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 52 - 111 4.8e-21 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 207
AciI CCGC 2 cut(s) 54, 84
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 439
AcyI GRCGYC 2 cut(s) 171, 231
AfaI GTAC 2 cut(s) 111, 514
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 3 cut(s) 305, 350, 446
AhdI GACNNNNNGTC 1 cut(s) 214
AluBI AGCT 5 cut(s) 65, 136, 247, 267, 276
AluI AGCT 5 cut(s) 65, 136, 247, 267, 276
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 371, 419
Ama87I CYCGRG 1 cut(s) 164
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 3 cut(s) 120, 175, 428
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 1 cut(s) 439
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 176
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 164
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 188
BceAI ACGGC 2 cut(s) 3, 244
BcoDI GTCTC 2 cut(s) 371, 419
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 2 cut(s) 81, 84
BlsI GCNGC 2 cut(s) 82, 85
BmeRI GACNNNNNGTC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 3 cut(s) 17, 178, 520
BmsI GCATC 2 cut(s) 322, 444
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 227, 323
BsaAI YACGTR 1 cut(s) 488
BsaHI GRCGYC 2 cut(s) 171, 231
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 250
BsaWI WCCGGW 1 cut(s) 106
Bse118I RCCGGY 1 cut(s) 430
Bse1I ACTGG 1 cut(s) 520
BseDI CCNNGG 1 cut(s) 250
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 426
BshFI GGCC 3 cut(s) 122, 177, 430
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 107, 431
BsmAI GTCTC 2 cut(s) 371, 419
BsnI GGCC 3 cut(s) 122, 177, 430
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 138
BspACI CCGC 2 cut(s) 54, 84
BspANI GGCC 3 cut(s) 122, 177, 430
BspLI GGNNCC 3 cut(s) 17, 178, 520
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 430
BsrI ACTGG 1 cut(s) 520
BssAI RCCGGY 1 cut(s) 430
BssECI CCNNGG 1 cut(s) 250
BssNI GRCGYC 2 cut(s) 171, 231
BssT1I CCWWGG 1 cut(s) 250
Bst4CI ACNGT 2 cut(s) 15, 385
BstACI GRCGYC 2 cut(s) 171, 231
BstAPI GCANNNNNTGC 2 cut(s) 80, 488
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 3 cut(s) 76, 409, 432
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstMAI GTCTC 2 cut(s) 371, 419
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 133, 273, 488
BstNSI RCATGY 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 295
BsuRI GGCC 3 cut(s) 122, 177, 430
Cac8I GCNNGC 3 cut(s) 76, 409, 432
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 430
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 220
Csp6I GTAC 2 cut(s) 110, 513
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 2 cut(s) 289, 469
CviJI RGCY 9 cut(s) 65, 122, 136, 177, 247, 257, 267, 276, 430
CviKI_1 RGCY 9 cut(s) 65, 122, 136, 177, 247, 257, 267, 276, 430
CviQI GTAC 2 cut(s) 110, 513
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 120
Eam1105I GACNNNNNGTC 1 cut(s) 214
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 292, 472
FaiI YATR 7 cut(s) 140, 224, 290, 395, 460, 462, 470
FatI CATG 2 cut(s) 288, 468
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 2 cut(s) 81, 84
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 81, 84
FspBI CTAG 1 cut(s) 273
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 81, 84
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 3 cut(s) 122, 177, 430
HapII CCGG 2 cut(s) 107, 431
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 80
Hin1I GRCGYC 2 cut(s) 171, 231
Hin1II CATG 2 cut(s) 292, 472
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 2 cut(s) 39, 89
HpaII CCGG 2 cut(s) 107, 431
Hpy166II GTNNAC 1 cut(s) 208
Hpy188III TCNNGA 2 cut(s) 164, 416
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 3 cut(s) 176, 209, 236
HpyCH4III ACNGT 2 cut(s) 15, 385
HpyCH4IV ACGT 3 cut(s) 171, 204, 487
HpyCH4V TGCA 3 cut(s) 335, 407, 482
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 133, 273, 488
HpySE526I ACGT 3 cut(s) 171, 204, 487
Hsp92I GRCGYC 2 cut(s) 171, 231
Hsp92II CATG 2 cut(s) 292, 472
HspAI GCGC 1 cut(s) 78
KroI GCCGGC 1 cut(s) 430
KroNI GCCGGC 1 cut(s) 432
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 4 cut(s) 120, 393, 444, 501
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 2 cut(s) 322, 444
MaeI CTAG 1 cut(s) 273
MaeII ACGT 3 cut(s) 171, 204, 487
MboII GAAGA 7 cut(s) 48, 84, 87, 90, 93, 377, 448
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 4 cut(s) 128, 300, 439, 477
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 3 cut(s) 39, 160, 420
MroNI GCCGGC 1 cut(s) 430
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 107, 431
MunI CAATTG 1 cut(s) 477
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 133, 273, 488
NaeI GCCGGC 1 cut(s) 432
NgoMIV GCCGGC 1 cut(s) 430
NlaIII CATG 2 cut(s) 292, 472
NlaIV GGNNCC 3 cut(s) 17, 178, 520
NmeAIII GCCGAG 2 cut(s) 98, 112
NspI RCATGY 1 cut(s) 472
NspV TTCGAA 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 432
PfeI GAWTC 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 82, 85
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Ppu21I YACGTR 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 17, 178, 520
PspPI GGNCC 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 2 cut(s) 111, 514
RsaNI GTAC 2 cut(s) 110, 513
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 2 cut(s) 81, 84
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 2 cut(s) 322, 444
Sfr274I CTCGAG 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 3 cut(s) 164, 242, 338
SmoI CTYRAG 3 cut(s) 164, 242, 338
Sse9I AATT 4 cut(s) 128, 300, 439, 477
SsiI CCGC 2 cut(s) 54, 84
SspMI CTAG 1 cut(s) 273
SstI GAGCTC 1 cut(s) 138
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 2 cut(s) 15, 385
TaiI ACGT 3 cut(s) 174, 207, 490
TaqI TCGA 4 cut(s) 68, 165, 207, 417
TasI AATT 4 cut(s) 128, 300, 439, 477
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 305, 378
TspGWI ACGGA 1 cut(s) 208
Tth111I GACNNNGTC 1 cut(s) 230
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 3 cut(s) 154, 257, 292
XhoI CTCGAG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 1 cut(s) 273
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.