Rw5G012200

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003568
Physical Location & Seq
Forward (+)
14290252 .. 14291203
952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012200.1

Sequence Viewer

Length: 726 bp
ATGGTTCCTTCTGATTTCTACTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAATGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAACGCAAGCGTTGCCGCCGAGTCTTCTTCTTCTTCTCTAGGACTCTACTTGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCAAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACATTGTGTCCGTCAAAGACGGCGTCATTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTTTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGGGGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGTTTATGATTGTAAGTGCAGGCAGTCTCGACACATTGAGGCCGGCGAAGAATTTTGAACTATTGATGCTATATGGTAGCATGTTGCCAATTGCAATACGTGCAGTTTTTTCTAACTCAAATAGGTACTGGAACCTTTTTGTTCCAAAAGTTCAGTATTTTTACTATTTTGTAAAGTATTGGTGTAATTATTCCACTTTTGTAGTTGTATACCGTGATAGTATAATGCCAATTTATACCATTTTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

241

Amino Acids

27.28

Weight (kDa)

8.79

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 82 - 140 2.5e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 2 cut(s) 294, 684
AciI CCGC 2 cut(s) 141, 171
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 2 cut(s) 258, 318
AfaI GTAC 1 cut(s) 602
AgsI TTSAA 3 cut(s) 392, 437, 533
AhdI GACNNNNNGTC 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 5 cut(s) 152, 223, 334, 354, 363
AluI AGCT 5 cut(s) 152, 223, 334, 354, 363
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 458, 506
AoxI GGCC 3 cut(s) 207, 262, 515
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspS9I GGNCC 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 155
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 225
BccI CCATC 2 cut(s) 78, 275
BceAI ACGGC 2 cut(s) 90, 331
BcoDI GTCTC 2 cut(s) 458, 506
BfaI CTAG 2 cut(s) 194, 360
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 4 cut(s) 6, 104, 265, 608
BmsI GCATC 2 cut(s) 409, 531
BpiI GAAGAC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 3 cut(s) 236, 314, 410
BsaAI YACGTR 1 cut(s) 575
BsaHI GRCGYC 2 cut(s) 258, 318
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 337
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 2 cut(s) 26, 608
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 1 cut(s) 424
BseNI ACTGG 2 cut(s) 26, 608
BseRI GAGGAG 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 2 cut(s) 513, 597
BshFI GGCC 3 cut(s) 209, 264, 517
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 1 cut(s) 518
BsmAI GTCTC 2 cut(s) 458, 506
BsnI GGCC 3 cut(s) 209, 264, 517
Bso31I GGTCTC 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 225
BspACI CCGC 2 cut(s) 141, 171
BspANI GGCC 3 cut(s) 209, 264, 517
BspLI GGNNCC 4 cut(s) 6, 104, 265, 608
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 2 cut(s) 26, 608
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 337
BssNAI GTATAC 1 cut(s) 685
BssNI GRCGYC 2 cut(s) 258, 318
BssT1I CCWWGG 1 cut(s) 337
Bst1107I GTATAC 1 cut(s) 685
Bst4CI ACNGT 2 cut(s) 472, 689
BstACI GRCGYC 2 cut(s) 258, 318
BstAPI GCANNNNNTGC 2 cut(s) 167, 575
BstBAI YACGTR 1 cut(s) 575
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 163, 496, 519
BstF5I GGATG 1 cut(s) 424
BstMAI GTCTC 2 cut(s) 458, 506
BstMWI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 360, 575
BstNSI RCATGY 1 cut(s) 559
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 382
BstZ17I GTATAC 1 cut(s) 685
BsuRI GGCC 3 cut(s) 209, 264, 517
BtsCI GGATG 1 cut(s) 424
Cac8I GCNNGC 3 cut(s) 163, 496, 519
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 263
CseI GACGC 1 cut(s) 307
Csp6I GTAC 1 cut(s) 601
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 2 cut(s) 376, 556
CviJI RGCY 9 cut(s) 152, 209, 223, 264, 334, 344, 354, 363, 517
CviKI_1 RGCY 9 cut(s) 152, 209, 223, 264, 334, 344, 354, 363, 517
CviQI GTAC 1 cut(s) 601
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 301
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 2 cut(s) 379, 559
FatI CATG 2 cut(s) 375, 555
FblI GTMKAC 2 cut(s) 294, 684
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 2 cut(s) 194, 360
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 3 cut(s) 209, 264, 517
HapII CCGG 1 cut(s) 518
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 2 cut(s) 258, 318
Hin1II CATG 2 cut(s) 379, 559
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 3 cut(s) 126, 176, 198
HpaII CCGG 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 295, 685
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 2 cut(s) 295, 685
Hpy99I CGWCG 2 cut(s) 263, 296
HpyAV CCTTC 2 cut(s) 18, 54
HpyCH4III ACNGT 2 cut(s) 472, 689
HpyCH4IV ACGT 3 cut(s) 258, 291, 574
HpyCH4V TGCA 4 cut(s) 422, 494, 569, 578
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 360, 575
HpySE526I ACGT 3 cut(s) 258, 291, 574
Hsp92I GRCGYC 2 cut(s) 258, 318
Hsp92II CATG 2 cut(s) 379, 559
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
LmnI GCTCC 1 cut(s) 339
LpnPI CCDG 5 cut(s) 7, 72, 480, 531, 589
LweI GCATC 2 cut(s) 409, 531
MaeI CTAG 2 cut(s) 194, 360
MaeII ACGT 3 cut(s) 258, 291, 574
MboII GAAGA 8 cut(s) 39, 135, 171, 174, 177, 180, 464, 535
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 225
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 6 cut(s) 39, 215, 526, 564, 661, 705
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 185, 192
MnlI CCTC 2 cut(s) 126, 507
Mox20I TGGCCA 1 cut(s) 209
MroNI GCCGGC 1 cut(s) 517
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 1 cut(s) 518
MunI CAATTG 1 cut(s) 564
MwoI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 360, 575
NaeI GCCGGC 1 cut(s) 519
NgoMIV GCCGGC 1 cut(s) 517
NlaIII CATG 2 cut(s) 379, 559
NlaIV GGNNCC 4 cut(s) 6, 104, 265, 608
NmeAIII GCCGAG 1 cut(s) 199
NspI RCATGY 1 cut(s) 559
NspV TTCGAA 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 2 cut(s) 184, 192
PpsI GAGTC 2 cut(s) 184, 192
Ppu21I YACGTR 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 4 cut(s) 6, 104, 265, 608
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 171
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 185, 192
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 2 cut(s) 409, 531
SfuI TTCGAA 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 251, 329, 425
SmoI CTYRAG 3 cut(s) 251, 329, 425
Sse9I AATT 6 cut(s) 39, 215, 526, 564, 661, 705
SsiI CCGC 2 cut(s) 141, 171
SspMI CTAG 2 cut(s) 194, 360
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 2 cut(s) 472, 689
TaiI ACGT 3 cut(s) 261, 294, 577
TaqI TCGA 3 cut(s) 155, 294, 504
TasI AATT 6 cut(s) 39, 215, 526, 564, 661, 705
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TspDTI ATGAA 2 cut(s) 392, 465
TspGWI ACGGA 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 317
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 3 cut(s) 241, 344, 379
XmiI GTMKAC 2 cut(s) 294, 684
XspI CTAG 2 cut(s) 194, 360
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.