Rh3AG278500

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003568
Physical Location & Seq
Reverse (-)
32378500 .. 32381569
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG278500.1

Sequence Viewer

Length: 543 bp
ATGGCGTCTCTCACCACCTCCTGGTTGCCCAAAACCCCAATTCCTTCTCCTCAAATCCCAGCGAAATCCCATCAGTCCCCACAACTTATGCTTGTAAAACGGTGCCGTTTAACTCCTCAAAGAAGGATCTTTCTGGGTTCCGCCATTAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTCGTCTTCTTCTTCTTCTTCATCTTCTCCGGGACTCTACTCGGCCAAGCAATTCGAGCTCACAGTTCCCAACGTCGACTTGGTCCTCGAGGACGTCCGCCCCTATCTCATCGCCGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAGAACAGAGAGACCACAGTTGAGGTGAGAGTTTTCGATTTCTCGACATGTTCATTCTGTGTTGATGGATTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

180

Amino Acids

19.41

Weight (kDa)

5.46

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 85 - 144 7e-24 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AbsI CCTCGAGG 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 21
AccI GTMKAC 2 cut(s) 252, 306
AciI CCGC 3 cut(s) 141, 171, 274
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 219
AcyI GRCGYC 3 cut(s) 5, 270, 330
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 21
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 2 cut(s) 404, 449
AhdI GACNNNNNGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 5 cut(s) 152, 235, 346, 366, 375
AluI AGCT 5 cut(s) 152, 235, 346, 366, 375
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 12, 470
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 263
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 167
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 4, 502
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 174, 330
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 154
BccI CCATC 3 cut(s) 78, 287, 524
BceAI ACGGC 2 cut(s) 90, 343
BciT130I CCWGG 1 cut(s) 22
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 12, 470
BfaI CTAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 2 cut(s) 168, 171
BlsI GCNGC 2 cut(s) 169, 172
Bme1390I CCNGG 2 cut(s) 22, 207
Bme18I GGWCC 1 cut(s) 259
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 2 cut(s) 104, 139
BmrFI CCNGG 2 cut(s) 22, 207
BmsI GCATC 1 cut(s) 421
BpiI GAAGAC 2 cut(s) 174, 330
BplI GAGNNNNNCTC 2 cut(s) 420, 452
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 326, 422
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 3 cut(s) 5, 270, 330
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 349
Bsc4I CCNNNNNNNGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 349
BseLI CCNNNNNNNGG 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 39, 105
BseXI GCAGC 1 cut(s) 154
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 2 cut(s) 61, 222
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 2 cut(s) 12, 470
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 61, 222
BsnI GGCC 1 cut(s) 221
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 3 cut(s) 141, 171, 274
BspANI GGCC 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 104, 139
BspPI GGATC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 3 cut(s) 5, 270, 330
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 102, 241, 484
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 3 cut(s) 5, 270, 330
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 12, 470
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
BstNI CCWGG 1 cut(s) 22
BstNSI RCATGY 1 cut(s) 516
BstSCI CCNGG 2 cut(s) 20, 205
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 2 cut(s) 174, 330
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 1 cut(s) 221
BtgZI GCGATG 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 259
CseI GACGC 1 cut(s) 319
CviAII CATG 2 cut(s) 388, 513
CviJI RGCY 7 cut(s) 152, 221, 235, 346, 356, 366, 375
CviKI_1 RGCY 7 cut(s) 152, 221, 235, 346, 356, 366, 375
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 2 cut(s) 130, 263
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 391, 516
FaiI YATR 3 cut(s) 89, 389, 514
FaqI GGGAC 2 cut(s) 61, 222
FatI CATG 2 cut(s) 387, 512
FblI GTMKAC 2 cut(s) 252, 306
Fnu4HI GCNGC 2 cut(s) 168, 171
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 168, 171
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 168, 171
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 221
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 319
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 3 cut(s) 5, 270, 330
Hin1II CATG 2 cut(s) 391, 516
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 2 cut(s) 253, 307
HindII GTYRAC 2 cut(s) 253, 307
HinfI GANTC 2 cut(s) 176, 210
HpaII CCGG 1 cut(s) 206
HphI GGTGA 2 cut(s) 4, 502
Hpy166II GTNNAC 2 cut(s) 253, 307
Hpy188III TCNNGA 2 cut(s) 508, 540
Hpy8I GTNNAC 2 cut(s) 253, 307
Hpy99I CGWCG 5 cut(s) 254, 308, 311, 323, 335
HpyAV CCTTC 2 cut(s) 54, 117
HpyCH4III ACNGT 3 cut(s) 102, 241, 484
HpyCH4IV ACGT 4 cut(s) 249, 270, 303, 309
HpyCH4V TGCA 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
HpySE526I ACGT 4 cut(s) 249, 270, 303, 309
Hsp92I GRCGYC 3 cut(s) 5, 270, 330
Hsp92II CATG 2 cut(s) 391, 516
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 351
LpnPI CCDG 5 cut(s) 7, 34, 72, 119, 219
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 372
MaeII ACGT 4 cut(s) 249, 270, 303, 309
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 8 cut(s) 174, 177, 180, 183, 186, 192, 335, 476
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 2 cut(s) 39, 227
MlyI GAGTC 2 cut(s) 185, 204
MnlI CCTC 6 cut(s) 28, 60, 126, 259, 272, 481
MseI TTAA 2 cut(s) 110, 414
MslI CAYNNNNRTG 1 cut(s) 392
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 22, 207
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 232, 372
NciI CCSGG 1 cut(s) 207
NdeII GATC 1 cut(s) 126
NlaIII CATG 2 cut(s) 391, 516
NlaIV GGNNCC 2 cut(s) 104, 139
NmeAIII GCCGAG 2 cut(s) 197, 199
NspI RCATGY 1 cut(s) 516
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 263
PciI ACATGT 1 cut(s) 512
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 327
PflFI GACNNNGTC 2 cut(s) 257, 329
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 2 cut(s) 169, 172
PleI GAGTC 2 cut(s) 184, 204
PpsI GAGTC 2 cut(s) 184, 204
PscI ACATGT 1 cut(s) 512
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 104, 139
PspPI GGNCC 1 cut(s) 259
PspXI VCTCGAGB 1 cut(s) 263
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 2 cut(s) 257, 329
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 2 cut(s) 251, 305
SaqAI TTAA 2 cut(s) 110, 414
SatI GCNGC 2 cut(s) 168, 171
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 2 cut(s) 185, 204
ScrFI CCNGG 2 cut(s) 22, 207
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 1 cut(s) 421
Sfr274I CTCGAG 1 cut(s) 263
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 305
SinI GGWCC 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 3 cut(s) 263, 341, 437
SmoI CTYRAG 3 cut(s) 263, 341, 437
Sse9I AATT 2 cut(s) 39, 227
SsiI CCGC 3 cut(s) 141, 171, 274
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 20, 205
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 3 cut(s) 102, 241, 484
TaiI ACGT 4 cut(s) 252, 273, 306, 312
TaqI TCGA 8 cut(s) 155, 231, 252, 264, 306, 321, 501, 509
TasI AATT 2 cut(s) 39, 227
TauI GCSGC 1 cut(s) 173
Tru1I TTAA 2 cut(s) 110, 414
Tru9I TTAA 2 cut(s) 110, 414
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 4 cut(s) 186, 404, 477, 507
TspGWI ACGGA 1 cut(s) 307
Tth111I GACNNNGTC 2 cut(s) 257, 329
Van91I CCANNNNNTGG 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 259
XceI RCATGY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 3 cut(s) 253, 356, 391
XhoI CTCGAG 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 252, 306
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.