pycom09g19370

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003568
Physical Location & Seq
Forward (+)
20205231 .. 20206958
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19370.1

Sequence Viewer

Length: 660 bp
ATGGCGTCTCTTGCGGCCACCGGGTTTCCCAAAACCCCAACGCCTTCTTCCAAAATCCCAGGTAAATCCCACCGATACCCACAATTTACATGTCTGAAACTGCACCGTCTCACCTCTCAAAGGCCGATCTTTCTCAAAACCGCTATTAGAGCTTCAAACCCAAGCGCTGCCGCCAAGTCTTCTTCTCCGGGATTCTACTCCGCCAAGCAGTACCAACTCACGGTTCCCAACGTCGACTTGGTTCTAGAGGACGTCCGCCCCTATCTCATCGCCGATGGCGGTAACGTCGATGTTGTCTCCGTCGAAGACGGCGTTGTTTCACTCAAGCTGCAAGGGGCATGTGGTAGCTGTCCGAGCTCAACGACCACCATGAAGATGGGAATCGAAAGAGTGCTCAAGGAGAAGTTTGGAGATGCGCTCAAGGACATTCAACAAGTCTTTGATGAAGAAAATAAAGAGATCACCGTTGAGGCAGTGAATCGTCATCTGGACATATTGAGACCAGCCATAAAGAACTTTGGCGGGAGTGTGGAAGTGTTATCGGTTGAAGGTGGGGATTGCCATGTTAATTATGTGGGGCCCGAGTCCATCGGATCAGGAATCAAAGCAGCAATTAAGGAGAAGTTCCCAGATATCGGCAATGTTGTGTTCACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

220

Amino Acids

23.47

Weight (kDa)

8.29

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 79 - 138 1e-23 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 234
AciI CCGC 7 cut(s) 14, 141, 171, 201, 256, 279, 522
AclWI GGATC 1 cut(s) 601
AcoI YGGCCR 1 cut(s) 15
AcyI GRCGYC 2 cut(s) 5, 252
AfaI GTAC 1 cut(s) 212
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 3 cut(s) 120, 220, 635
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 3 cut(s) 156, 431, 548
AjnI CCWGG 1 cut(s) 58
AjuI GAANNNNNNNTTGG 4 cut(s) 31, 63, 207, 239
AluBI AGCT 4 cut(s) 152, 328, 348, 357
AluI AGCT 4 cut(s) 152, 328, 348, 357
Alw21I GWGCWC 2 cut(s) 359, 396
Alw26I GTCTC 4 cut(s) 12, 113, 301, 493
AlwI GGATC 1 cut(s) 601
Ama87I CYCGRG 1 cut(s) 581
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 3 cut(s) 15, 122, 578
ApaI GGGCCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 167, 328, 608
AspLEI GCGC 2 cut(s) 167, 418
AspS9I GGNCC 2 cut(s) 578, 579
AsuC2I CCSGG 2 cut(s) 22, 189
AsuHPI GGTGA 2 cut(s) 103, 454
AvaI CYCGRG 1 cut(s) 581
BaeGI GKGCMC 1 cut(s) 582
BanII GRGCYC 2 cut(s) 359, 582
BbsI GAAGAC 2 cut(s) 171, 312
Bbv12I GWGCWC 2 cut(s) 359, 396
BbvI GCAGC 3 cut(s) 154, 315, 620
BccI CCATC 3 cut(s) 269, 370, 596
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 60
BcnI CCSGG 2 cut(s) 22, 189
BcoDI GTCTC 4 cut(s) 12, 113, 301, 493
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 5 cut(s) 15, 168, 171, 329, 609
BlsI GCNGC 5 cut(s) 16, 169, 172, 330, 610
Bme1390I CCNGG 3 cut(s) 22, 60, 189
BmeT110I CYCGRG 1 cut(s) 581
BmgT120I GGNCC 2 cut(s) 578, 579
BmiI GGNNCC 3 cut(s) 225, 579, 580
BmrFI CCNGG 3 cut(s) 22, 60, 189
BmsI GCATC 1 cut(s) 403
BpiI GAAGAC 2 cut(s) 171, 312
BplI GAGNNNNNCTC 2 cut(s) 402, 434
BpuEI CTTGAG 3 cut(s) 308, 380, 404
BpuMI CCSGG 2 cut(s) 22, 189
BsaBI GATNNNNATC 1 cut(s) 380
BsaHI GRCGYC 2 cut(s) 5, 252
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 220, 635
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 646
Bse8I GATNNNNATC 1 cut(s) 380
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 3 cut(s) 120, 220, 635
BseMI GCAATG 1 cut(s) 646
BseNI ACTGG 1 cut(s) 658
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 3 cut(s) 154, 315, 620
BsgI GTGCAG 1 cut(s) 86
BshFI GGCC 3 cut(s) 17, 124, 580
BsiHKAI GWGCWC 2 cut(s) 359, 396
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 2 cut(s) 21, 188
BslI CCNNNNNNNGG 3 cut(s) 120, 220, 635
BsmAI GTCTC 4 cut(s) 12, 113, 301, 493
BsmBI CGTCTC 2 cut(s) 12, 113
BsnI GGCC 3 cut(s) 17, 124, 580
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 581
Bsp120I GGGCCC 1 cut(s) 578
Bsp1286I GDGCHC 3 cut(s) 359, 396, 582
Bsp143I GATC 3 cut(s) 126, 459, 593
BspACI CCGC 7 cut(s) 14, 141, 171, 201, 256, 279, 522
BspANI GGCC 3 cut(s) 17, 124, 580
BspLI GGNNCC 3 cut(s) 225, 579, 580
BspPI GGATC 1 cut(s) 601
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 646
BsrI ACTGG 1 cut(s) 658
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 126, 459, 593
BssNI GRCGYC 2 cut(s) 5, 252
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 3 cut(s) 107, 223, 466
BstACI GRCGYC 2 cut(s) 5, 252
BstENI CCTNNNNNAGG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 167, 418
BstKTI GATC 3 cut(s) 129, 462, 596
BstMAI GTCTC 4 cut(s) 12, 113, 301, 493
BstMBI GATC 3 cut(s) 126, 459, 593
BstMWI GCNNNNNNNGC 3 cut(s) 11, 149, 354
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 2 cut(s) 93, 342
BstSCI CCNGG 3 cut(s) 20, 58, 187
BstSLI GKGCMC 1 cut(s) 582
BstV1I GCAGC 3 cut(s) 154, 315, 620
BstV2I GAAGAC 2 cut(s) 171, 312
BstXI CCANNNNNNTGG 1 cut(s) 376
BsuRI GGCC 3 cut(s) 17, 124, 580
BtgZI GCGATG 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 480, 651
CfoI GCGC 2 cut(s) 167, 418
Cfr13I GGNCC 2 cut(s) 578, 579
Csp6I GTAC 1 cut(s) 211
CviAII CATG 4 cut(s) 90, 339, 370, 563
CviJI RGCY 8 cut(s) 17, 124, 152, 328, 348, 357, 506, 580
CviKI_1 RGCY 8 cut(s) 17, 124, 152, 328, 348, 357, 506, 580
CviQI GTAC 1 cut(s) 211
DpnI GATC 3 cut(s) 128, 461, 595
DpnII GATC 3 cut(s) 126, 459, 593
EaeI YGGCCR 1 cut(s) 15
EciI GGCGGA 2 cut(s) 190, 245
Ecl136II GAGCTC 1 cut(s) 357
Eco24I GRGCYC 2 cut(s) 359, 582
Eco31I GGTCTC 1 cut(s) 493
Eco32I GATATC 1 cut(s) 634
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 58
EcoRV GATATC 1 cut(s) 634
EcoT38I GRGCYC 2 cut(s) 359, 582
Esp3I CGTCTC 2 cut(s) 12, 113
FaeI CATG 4 cut(s) 93, 342, 373, 566
FaiI YATR 7 cut(s) 91, 340, 371, 494, 509, 564, 573
FatI CATG 4 cut(s) 89, 338, 369, 562
FauI CCCGC 1 cut(s) 515
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 5 cut(s) 15, 168, 171, 329, 609
FriOI GRGCYC 2 cut(s) 359, 582
Fsp4HI GCNGC 5 cut(s) 15, 168, 171, 329, 609
FspBI CTAG 1 cut(s) 245
GlaI GCGC 2 cut(s) 166, 417
GluI GCNGC 5 cut(s) 15, 168, 171, 329, 609
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 3 cut(s) 17, 124, 580
HapII CCGG 2 cut(s) 21, 188
HhaI GCGC 2 cut(s) 167, 418
Hin1I GRCGYC 2 cut(s) 5, 252
Hin1II CATG 4 cut(s) 93, 342, 373, 566
Hin6I GCGC 2 cut(s) 165, 416
HinP1I GCGC 2 cut(s) 165, 416
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 5 cut(s) 192, 381, 478, 584, 600
HpaII CCGG 2 cut(s) 21, 188
HphI GGTGA 2 cut(s) 103, 454
Hpy166II GTNNAC 2 cut(s) 235, 651
Hpy188I TCNGA 3 cut(s) 96, 354, 593
Hpy188III TCNNGA 3 cut(s) 245, 488, 597
Hpy8I GTNNAC 2 cut(s) 235, 651
Hpy99I CGWCG 3 cut(s) 236, 290, 305
HpyAV CCTTC 2 cut(s) 54, 542
HpyCH4III ACNGT 3 cut(s) 107, 223, 466
HpyCH4IV ACGT 3 cut(s) 231, 252, 285
HpyCH4V TGCA 2 cut(s) 103, 331
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 149, 354
HpySE526I ACGT 3 cut(s) 231, 252, 285
Hsp92I GRCGYC 2 cut(s) 5, 252
Hsp92II CATG 4 cut(s) 93, 342, 373, 566
HspAI GCGC 2 cut(s) 165, 416
Kzo9I GATC 3 cut(s) 126, 459, 593
LpnPI CCDG 9 cut(s) 34, 45, 72, 201, 473, 516, 582, 639, 642
Lsp1109I GCAGC 3 cut(s) 154, 315, 620
LweI GCATC 1 cut(s) 403
MaeI CTAG 1 cut(s) 245
MaeII ACGT 3 cut(s) 231, 252, 285
MaeIII GTNAC 1 cut(s) 281
MalI GATC 3 cut(s) 128, 461, 595
MboI GATC 3 cut(s) 126, 459, 593
MboII GAAGA 6 cut(s) 39, 171, 174, 317, 385, 458
MhlI GDGCHC 3 cut(s) 359, 396, 582
MluCI AATT 3 cut(s) 83, 568, 612
MlyI GAGTC 1 cut(s) 593
MnlI CCTC 3 cut(s) 124, 241, 463
MseI TTAA 2 cut(s) 567, 615
MslI CAYNNNNRTG 1 cut(s) 374
MspI CCGG 2 cut(s) 21, 188
MspR9I CCNGG 3 cut(s) 22, 60, 189
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 11, 149, 354
NciI CCSGG 2 cut(s) 22, 189
NdeII GATC 3 cut(s) 126, 459, 593
NlaIII CATG 4 cut(s) 93, 342, 373, 566
NlaIV GGNNCC 3 cut(s) 225, 579, 580
NspI RCATGY 2 cut(s) 93, 342
PciI ACATGT 1 cut(s) 89
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PfeI GAWTC 4 cut(s) 192, 381, 478, 600
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 5 cut(s) 16, 169, 172, 330, 610
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PscI ACATGT 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 225, 579, 580
PspOMI GGGCCC 1 cut(s) 578
PspPI GGNCC 2 cut(s) 578, 579
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 359
SalI GTCGAC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 567, 615
SatI GCNGC 5 cut(s) 15, 168, 171, 329, 609
Sau3AI GATC 3 cut(s) 126, 459, 593
Sau96I GGNCC 2 cut(s) 578, 579
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 3 cut(s) 22, 60, 189
SduI GDGCHC 3 cut(s) 359, 396, 582
SfaNI GCATC 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 3 cut(s) 323, 395, 419
SmoI CTYRAG 3 cut(s) 323, 395, 419
Sse9I AATT 3 cut(s) 83, 568, 612
SsiI CCGC 7 cut(s) 14, 141, 171, 201, 256, 279, 522
SspMI CTAG 1 cut(s) 245
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 3 cut(s) 20, 58, 187
TaaI ACNGT 3 cut(s) 107, 223, 466
TaiI ACGT 3 cut(s) 234, 255, 288
TaqI TCGA 4 cut(s) 234, 288, 303, 384
TasI AATT 3 cut(s) 83, 568, 612
TauI GCSGC 2 cut(s) 17, 173
TfiI GAWTC 4 cut(s) 192, 381, 478, 600
Tru1I TTAA 2 cut(s) 567, 615
Tru9I TTAA 2 cut(s) 567, 615
TscAI CASTG 2 cut(s) 480, 658
TseI GCWGC 3 cut(s) 167, 328, 608
TspDTI ATGAA 2 cut(s) 386, 459
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 2 cut(s) 480, 658
XagI CCTNNNNNAGG 1 cut(s) 118
XbaI TCTAGA 1 cut(s) 244
XceI RCATGY 2 cut(s) 93, 342
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 373
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 1 cut(s) 245
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.