AT4G31300

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003666
Physical Location & Seq
Forward (+)
15188744 .. 15191217
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31300.1

Sequence Viewer

Length: 702 bp
ATGGATCTCAATCTCGATGCACCTCACTCCATGGGAACGACCATCATCGGCGTCACTTACAATGGAGGTGTCGTCCTCGGAGCCGACTCACGTACCAGCACCGGTATGTACGTAGCGAATCGAGCTTCTGATAAGATTACACAACTGACAGATAATGTCTACGTCTGCCGCTCTGGATCGGCTGCTGATTCTCAAGTTGTATCTGACTATGTCCGCTACTTCCTTCACCAGCATACAATTCAGCATGGACAACCCGCAACTGTTAAGGTTTCTGCCAACCTTATCAGGATGCTCGCATATAATAACAAGAACATGCTCCAAACTGGTCTCATCGTTGGAGGATGGGATAAATATGAGGGTGGGAAGATCTACGGGATCCCACTCGGTGGAACTGTAGTCGAGCAACCGTTTGCCATTGGAGGCTCTGGTTCGAGCTATCTCTACGGGTTCTTCGACCAGGCTTGGAAAGATAACATGACCAAAGAAGAAGCAGAGCAACTTGTTGTGAAGGCGGTTTCACTAGCCATCGCCCGTGATGGAGCCAGTGGTGGTGTCGTACGGACTGTCATAATCAACTCAGAGGGAGTGACGAGAAACTTTTACCCTGGAGATAAGTTGCAGCTTTGGCACGAAGAATTGGAGCCCCAAAACTCACTCTTAGACATTCTGAACGCTGCTGGTCCTGAACCAATGGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

233

Amino Acids

25.15

Weight (kDa)

5.31

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 11 - 191 8.9e-52 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 386
AccBSI CCGCTC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 159
AciI CCGC 4 cut(s) 169, 214, 255, 512
AclWI GGATC 4 cut(s) 12, 184, 370, 383
AcoI YGGCCR 1 cut(s) 693
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 386
AfaI GTAC 3 cut(s) 94, 110, 558
AfiI CCNNNNNNNGG 1 cut(s) 386
AgeI ACCGGT 1 cut(s) 101
AjnI CCWGG 2 cut(s) 456, 604
AluBI AGCT 3 cut(s) 125, 435, 622
AluI AGCT 3 cut(s) 125, 435, 622
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 4 cut(s) 12, 184, 370, 383
AoxI GGCC 1 cut(s) 693
ApeKI GCWGC 3 cut(s) 182, 619, 674
AsiGI ACCGGT 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 680
BaeI ACNNNNGTAYC 2 cut(s) 76, 109
BalI TGGCCA 1 cut(s) 695
BamHI GGATCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 169, 631, 661
BccI CCATC 4 cut(s) 50, 336, 530, 533
BciT130I CCWGG 2 cut(s) 458, 606
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 1 cut(s) 393
BglII AGATCT 1 cut(s) 366
BisI GCNGC 4 cut(s) 169, 183, 620, 675
BlsI GCNGC 4 cut(s) 170, 184, 621, 676
Bme1390I CCNGG 2 cut(s) 458, 606
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 4 cut(s) 82, 377, 541, 642
BmrFI CCNGG 2 cut(s) 458, 606
BmsI GCATC 2 cut(s) 7, 279
BpmI CTGGAG 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 177
BsaAI YACGTR 2 cut(s) 92, 112
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 30, 76, 604
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 328, 543
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 2 cut(s) 458, 606
BseDI CCNNGG 3 cut(s) 30, 76, 604
BseGI GGATG 2 cut(s) 294, 347
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 328, 543
BseXI GCAGC 3 cut(s) 169, 631, 661
BshFI GGCC 1 cut(s) 695
BshTI ACCGGT 1 cut(s) 101
BsiSI CCGG 1 cut(s) 102
BsiWI CGTACG 1 cut(s) 556
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 332
BsnI GGCC 1 cut(s) 695
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 645
Bsp143I GATC 4 cut(s) 4, 176, 366, 375
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 4 cut(s) 169, 214, 255, 512
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 1 cut(s) 590
BspLI GGNNCC 4 cut(s) 82, 377, 541, 642
BspPI GGATC 4 cut(s) 12, 184, 370, 383
BspTNI GGTCTC 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 2 cut(s) 328, 543
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 30, 76, 604
BssMI GATC 4 cut(s) 4, 176, 366, 375
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 458, 606
Bst4CI ACNGT 4 cut(s) 262, 394, 408, 565
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 2 cut(s) 92, 112
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 577, 658
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 294, 347
BstKTI GATC 4 cut(s) 7, 179, 369, 378
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 4 cut(s) 4, 176, 366, 375
BstMWI GCNNNNNNNGC 2 cut(s) 122, 625
BstNI CCWGG 2 cut(s) 458, 606
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 456, 604
BstSFI CTRYAG 1 cut(s) 393
BstSNI TACGTA 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 169, 631, 661
BstX2I RGATCY 3 cut(s) 4, 366, 375
BstYI RGATCY 3 cut(s) 4, 366, 375
BsuRI GGCC 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 294, 347
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 680
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 93, 109, 557
CspAI ACCGGT 1 cut(s) 101
CviAII CATG 5 cut(s) 31, 245, 313, 475, 697
CviQI GTAC 3 cut(s) 93, 109, 557
DdeI CTNAG 2 cut(s) 577, 658
DpnI GATC 4 cut(s) 6, 178, 368, 377
DpnII GATC 4 cut(s) 4, 176, 366, 375
DraIII CACNNNGTG 1 cut(s) 386
EaeI YGGCCR 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 456, 604
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 5 cut(s) 34, 248, 316, 478, 700
FatI CATG 5 cut(s) 30, 244, 312, 474, 696
FauI CCCGC 1 cut(s) 262
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 169, 183, 620, 675
FokI GGATG 2 cut(s) 301, 354
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 4 cut(s) 169, 183, 620, 675
FspBI CTAG 1 cut(s) 521
GluI GCNGC 4 cut(s) 169, 183, 620, 675
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 1 cut(s) 695
HapII CCGG 1 cut(s) 102
HgaI GACGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 34, 248, 316, 478, 700
HinfI GANTC 3 cut(s) 86, 118, 188
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 5 cut(s) 80, 130, 205, 580, 669
Hpy188III TCNNGA 4 cut(s) 14, 174, 286, 683
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 233, 502
HpyCH4III ACNGT 4 cut(s) 262, 394, 408, 565
HpyCH4IV ACGT 3 cut(s) 91, 111, 162
HpyCH4V TGCA 2 cut(s) 20, 619
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 625
HpyF3I CTNAG 2 cut(s) 577, 658
HpySE526I ACGT 3 cut(s) 91, 111, 162
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 34, 248, 316, 478, 700
Kzo9I GATC 4 cut(s) 4, 176, 366, 375
LmnI GCTCC 4 cut(s) 80, 321, 539, 640
Lsp1109I GCAGC 3 cut(s) 169, 631, 661
LweI GCATC 2 cut(s) 7, 279
MaeI CTAG 1 cut(s) 521
MaeII ACGT 3 cut(s) 91, 111, 162
MaeIII GTNAC 2 cut(s) 52, 586
MalI GATC 4 cut(s) 6, 178, 368, 377
MbiI CCGCTC 1 cut(s) 171
MboI GATC 4 cut(s) 4, 176, 366, 375
MboII GAAGA 4 cut(s) 376, 442, 497, 644
MflI RGATCY 3 cut(s) 4, 366, 375
MhlI GDGCHC 1 cut(s) 645
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 2 cut(s) 237, 635
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 7 cut(s) 33, 59, 86, 332, 349, 413, 574
Mox20I TGGCCA 1 cut(s) 695
MscI TGGCCA 1 cut(s) 695
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 104
Msp20I TGGCCA 1 cut(s) 695
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 2 cut(s) 458, 606
MvaI CCWGG 2 cut(s) 458, 606
MwoI GCNNNNNNNGC 2 cut(s) 122, 625
NcoI CCATGG 1 cut(s) 30
NdeII GATC 4 cut(s) 4, 176, 366, 375
NlaIII CATG 5 cut(s) 34, 248, 316, 478, 700
NlaIV GGNNCC 4 cut(s) 82, 377, 541, 642
NmuCI GTSAC 2 cut(s) 52, 586
NspI RCATGY 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PfeI GAWTC 2 cut(s) 118, 188
Pfl23II CGTACG 1 cut(s) 556
PflFI GACNNNGTC 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 386
PinAI ACCGGT 1 cut(s) 101
PkrI GCNGC 4 cut(s) 170, 184, 621, 676
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 2 cut(s) 92, 112
Psp6I CCWGG 2 cut(s) 456, 604
PspGI CCWGG 2 cut(s) 456, 604
PspLI CGTACG 1 cut(s) 556
PspN4I GGNNCC 4 cut(s) 82, 377, 541, 642
PspPI GGNCC 1 cut(s) 680
PsuI RGATCY 3 cut(s) 4, 366, 375
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 3 cut(s) 94, 110, 558
RsaNI GTAC 3 cut(s) 93, 109, 557
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 4 cut(s) 169, 183, 620, 675
Sau3AI GATC 4 cut(s) 4, 176, 366, 375
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 458, 606
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 2 cut(s) 7, 279
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
SnaBI TACGTA 1 cut(s) 112
Sse9I AATT 2 cut(s) 237, 635
SsiI CCGC 4 cut(s) 169, 214, 255, 512
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 2 cut(s) 456, 604
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 4 cut(s) 262, 394, 408, 565
TaiI ACGT 3 cut(s) 94, 114, 165
TaqI TCGA 5 cut(s) 15, 121, 399, 431, 453
TasI AATT 2 cut(s) 237, 635
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 2 cut(s) 118, 188
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 2 cut(s) 52, 586
TseI GCWGC 3 cut(s) 182, 619, 674
Tsp45I GTSAC 2 cut(s) 52, 586
TspGWI ACGGA 1 cut(s) 574
TspRI CASTG 1 cut(s) 550
Tth111I GACNNNGTC 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 680
XceI RCATGY 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.