MD08G1124600.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003666
Physical Location & Seq
Reverse (-)
11679806 .. 11682427
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1124600.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCTTCTTCCGACATGGATCTCAACGCCCCTCACTCCATGGGTACCACCATCATCGGCGTCACCTACGACGGAGGCGTCGTCCTTGGCGCCGACTCTCGTACCAGCACCGGAGTATACGTGGCCAATCGCGCCTCCGACAAAATCACCCAGCTCACCGACAATGTCTACGTCTGCCGCTCTGGATCGGCTGCCGATTCCCAGGTTGTTTCCGATTACGTTCGTTACTTCCTTCATCAGCACACGTCAGTAATTCAGCTTGGACAACCTGCGACTGTCAAAGTTTGTGCAAACCTTGTCAGGCTGCTGTCCTATGGTAACAAGAATATGTTGGAAACTGGACTGATTGTTGGCGGGTGGGACAAGTACGAAGGCGGTAAGATTTATGGGATTCCTCTTGGTGGCACACTGCTAGAGCTGCCCTTTGCCATTGGAGGATCTGGCTCCAGTTACTTGTATGGATTTTTCGATCAAGCATGGAAAGAAGGAATGACCAAGGACGAAGCTGAGCAATTGGTGGTCAAGGCTGTTTCTCTCGCCATTGCACGAGATGGTGCCAGTGGGGGTGTTGTCCGTACTGTAGTTATAAATTCTGAGGGAGTGACAAGAAACTTCTATCCTGGCGACAAACTTCCACTGTGGCATGAGGAGTTGGAGCCTCAGAACTCATTGTTGGACATATTGAACACTGCTAGTCCCGAGCCAATGAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

239

Amino Acids

25.49

Weight (kDa)

4.94

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 14 - 196 6e-47 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 277
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 3 cut(s) 44, 89, 554
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 2 cut(s) 117, 168
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 178, 354, 375
AclWI GGATC 3 cut(s) 27, 193, 445
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 589
AcyI GRCGYC 3 cut(s) 60, 78, 90
AfaI GTAC 4 cut(s) 46, 103, 368, 577
AfiI CCNNNNNNNGG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 1 cut(s) 685
AjiI CACGTC 1 cut(s) 246
AjnI CCWGG 2 cut(s) 201, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AluBI AGCT 4 cut(s) 154, 259, 418, 506
AluI AGCT 4 cut(s) 154, 259, 418, 506
AlwI GGATC 3 cut(s) 27, 193, 445
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 191, 304, 418
ApoI RAATTY 1 cut(s) 589
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 2 cut(s) 92, 134
AsuHPI GGTGA 3 cut(s) 55, 139, 148
AvaI CYCGRG 1 cut(s) 698
BaeI ACNNNNGTAYC 2 cut(s) 85, 118
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 3 cut(s) 44, 89, 554
BauI CACGAG 1 cut(s) 546
BbvI GCAGC 3 cut(s) 178, 291, 405
BccI CCATC 2 cut(s) 59, 545
BciT130I CCWGG 2 cut(s) 203, 621
BfaI CTAG 2 cut(s) 413, 693
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 4 cut(s) 178, 192, 305, 419
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 4 cut(s) 179, 193, 306, 420
Bme1390I CCNGG 2 cut(s) 203, 621
BmeT110I CYCGRG 1 cut(s) 698
BmgBI CACGTC 1 cut(s) 246
BmiI GGNNCC 5 cut(s) 46, 91, 445, 556, 657
BmrFI CCNGG 2 cut(s) 203, 621
BpmI CTGGAG 1 cut(s) 430
Bpu1102I GCTNAGC 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 3 cut(s) 60, 78, 90
BsaJI CCNNGG 4 cut(s) 39, 85, 201, 495
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 3 cut(s) 343, 447, 558
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 2 cut(s) 203, 621
BseDI CCNNGG 4 cut(s) 39, 85, 201, 495
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 498, 585, 674
BseNI ACTGG 3 cut(s) 343, 447, 558
BseRI GAGGAG 1 cut(s) 662
BseXI GCAGC 3 cut(s) 178, 291, 405
BseYI CCCAGC 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 125
BshNI GGYRCC 3 cut(s) 44, 89, 554
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 2 cut(s) 374, 681
BslI CCNNNNNNNGG 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 374, 681
BsnI GGCC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 698
Bsp143I GATC 4 cut(s) 19, 185, 437, 469
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 3 cut(s) 178, 354, 375
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 3 cut(s) 499, 586, 673
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 46, 91, 445, 556, 657
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 3 cut(s) 27, 193, 445
BspT107I GGYRCC 3 cut(s) 44, 89, 554
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 3 cut(s) 343, 447, 558
BssECI CCNNGG 4 cut(s) 39, 85, 201, 495
BssMI GATC 4 cut(s) 19, 185, 437, 469
BssNAI GTATAC 1 cut(s) 118
BssNI GRCGYC 3 cut(s) 60, 78, 90
BssSI CACGAG 1 cut(s) 546
BssT1I CCWWGG 3 cut(s) 39, 85, 495
Bst1107I GTATAC 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 2 cut(s) 203, 621
Bst4CI ACNGT 3 cut(s) 277, 580, 639
BstACI GRCGYC 3 cut(s) 60, 78, 90
BstBAI YACGTR 1 cut(s) 121
BstDEI CTNAG 3 cut(s) 507, 594, 660
BstDSI CCRYGG 1 cut(s) 39
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 134
BstKTI GATC 4 cut(s) 22, 188, 440, 472
BstMBI GATC 4 cut(s) 19, 185, 437, 469
BstMWI GCNNNNNNNGC 2 cut(s) 131, 418
BstNI CCWGG 2 cut(s) 203, 621
BstSCI CCNGG 2 cut(s) 201, 619
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 178, 291, 405
BstX2I RGATCY 2 cut(s) 19, 437
BstYI RGATCY 2 cut(s) 19, 437
BstZ17I GTATAC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 246
BtsI GCAGTG 2 cut(s) 407, 687
BtsIMutI CAGTG 4 cut(s) 407, 565, 635, 687
BveI ACCTGC 1 cut(s) 277
CfoI GCGC 2 cut(s) 92, 134
CseI GACGC 2 cut(s) 49, 67
Csp6I GTAC 4 cut(s) 45, 102, 367, 576
CviAII CATG 4 cut(s) 16, 40, 477, 644
CviQI GTAC 4 cut(s) 45, 102, 367, 576
DdeI CTNAG 3 cut(s) 507, 594, 660
DinI GGCGCC 1 cut(s) 91
DpnI GATC 4 cut(s) 21, 187, 439, 471
DpnII GATC 4 cut(s) 19, 185, 437, 469
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 123
Eco130I CCWWGG 3 cut(s) 39, 85, 495
Eco88I CYCGRG 1 cut(s) 698
EcoRII CCWGG 2 cut(s) 201, 619
EcoT14I CCWWGG 3 cut(s) 39, 85, 495
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 39, 85, 495
FaeI CATG 4 cut(s) 19, 43, 480, 647
FaqI GGGAC 2 cut(s) 374, 681
FatI CATG 4 cut(s) 15, 39, 476, 643
FauI CCCGC 1 cut(s) 347
FauNDI CATATG 1 cut(s) 713
FblI GTMKAC 2 cut(s) 117, 168
Fnu4HI GCNGC 4 cut(s) 178, 192, 305, 419
Fsp4HI GCNGC 4 cut(s) 178, 192, 305, 419
FspBI CTAG 2 cut(s) 413, 693
GlaI GCGC 2 cut(s) 91, 133
GluI GCNGC 4 cut(s) 178, 192, 305, 419
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 430
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 111
HgaI GACGC 2 cut(s) 49, 67
HhaI GCGC 2 cut(s) 92, 134
Hin1I GRCGYC 3 cut(s) 60, 78, 90
Hin1II CATG 4 cut(s) 19, 43, 480, 647
Hin6I GCGC 2 cut(s) 90, 132
HinP1I GCGC 2 cut(s) 90, 132
HinfI GANTC 3 cut(s) 95, 197, 391
HpaII CCGG 1 cut(s) 111
HphI GGTGA 3 cut(s) 55, 139, 148
Hpy166II GTNNAC 2 cut(s) 118, 169
Hpy188I TCNGA 5 cut(s) 13, 139, 214, 595, 663
Hpy188III TCNNGA 2 cut(s) 183, 698
Hpy8I GTNNAC 2 cut(s) 118, 169
Hpy99I CGWCG 2 cut(s) 74, 83
HpyAV CCTTC 3 cut(s) 242, 365, 479
HpyCH4III ACNGT 3 cut(s) 277, 580, 639
HpyCH4IV ACGT 4 cut(s) 120, 171, 219, 245
HpyCH4V TGCA 2 cut(s) 290, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 418
HpyF3I CTNAG 3 cut(s) 507, 594, 660
HpySE526I ACGT 4 cut(s) 120, 171, 219, 245
Hsp92I GRCGYC 3 cut(s) 60, 78, 90
Hsp92II CATG 4 cut(s) 19, 43, 480, 647
HspAI GCGC 2 cut(s) 90, 132
KasI GGCGCC 1 cut(s) 89
KpnI GGTACC 1 cut(s) 48
Kzo9I GATC 4 cut(s) 19, 185, 437, 469
LmnI GCTCC 2 cut(s) 449, 655
Lsp1109I GCAGC 3 cut(s) 178, 291, 405
MaeI CTAG 2 cut(s) 413, 693
MaeII ACGT 4 cut(s) 120, 171, 219, 245
MaeIII GTNAC 5 cut(s) 61, 224, 317, 449, 601
MalI GATC 4 cut(s) 21, 187, 439, 471
MbiI CCGCTC 1 cut(s) 180
MboI GATC 4 cut(s) 19, 185, 437, 469
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 2 cut(s) 19, 437
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 3 cut(s) 252, 512, 589
MluNI TGGCCA 1 cut(s) 125
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 36, 162, 312, 633, 654
MnlI CCTC 8 cut(s) 42, 68, 145, 405, 428, 589, 640, 669
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 203, 621
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 2 cut(s) 203, 621
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 131, 418
NarI GGCGCC 1 cut(s) 90
NcoI CCATGG 1 cut(s) 39
NdeI CATATG 1 cut(s) 713
NdeII GATC 4 cut(s) 19, 185, 437, 469
NlaIII CATG 4 cut(s) 19, 43, 480, 647
NlaIV GGNNCC 5 cut(s) 46, 91, 445, 556, 657
NmuCI GTSAC 2 cut(s) 61, 601
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 78
PfeI GAWTC 2 cut(s) 197, 391
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 4 cut(s) 179, 193, 306, 420
PleI GAGTC 1 cut(s) 89
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 121
PsiI TTATAA 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 201, 619
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 2 cut(s) 201, 619
PspN4I GGNNCC 5 cut(s) 46, 91, 445, 556, 657
PsuI RGATCY 2 cut(s) 19, 437
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 4 cut(s) 46, 103, 368, 577
RsaNI GTAC 4 cut(s) 45, 102, 367, 576
SatI GCNGC 4 cut(s) 178, 192, 305, 419
Sau3AI GATC 4 cut(s) 19, 185, 437, 469
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 203, 621
SfcI CTRYAG 1 cut(s) 579
SfoI GGCGCC 1 cut(s) 91
Sse9I AATT 3 cut(s) 252, 512, 589
SsiI CCGC 3 cut(s) 178, 354, 375
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 2 cut(s) 413, 693
StyD4I CCNGG 2 cut(s) 201, 619
StyI CCWWGG 3 cut(s) 39, 85, 495
TaaI ACNGT 3 cut(s) 277, 580, 639
TaiI ACGT 4 cut(s) 123, 174, 222, 248
TaqI TCGA 1 cut(s) 468
TasI AATT 3 cut(s) 252, 512, 589
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 197, 391
TscAI CASTG 4 cut(s) 414, 565, 642, 694
TseFI GTSAC 2 cut(s) 61, 601
TseI GCWGC 3 cut(s) 191, 304, 418
Tsp45I GTSAC 2 cut(s) 61, 601
TspDTI ATGAA 1 cut(s) 224
TspGWI ACGGA 2 cut(s) 87, 563
TspRI CASTG 4 cut(s) 414, 565, 642, 694
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 589
XmiI GTMKAC 2 cut(s) 117, 168
XspI CTAG 2 cut(s) 413, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.