Rroxscaffold_7G00162840

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003666
Physical Location & Seq
Reverse (-)
5215214 .. 5217600
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162840.1

Sequence Viewer

Length: 726 bp
ATGTCTCACTCATCAATGGACGATATGAACCTCAACGCCCCTCACTCCATGGGCACCACCATCATCGGCGTCACCTACGACGGCGGCGTCGTCCTCGGCGCCGACTCCCGAACCTCCACCGGTGTCTATGTTGCGAATCGGTGCTCGGATAAAATCACTCAGCTCACGGATAATGTCTACGTCTGTCGCTCTGGATCGGCTGCAGATTCTCAGGTTGTTTCCGACTATGTGAGGCACTTTCTTCATCAGCACACCATTCAGCTAGGGCAGCCTGCTACTGTCAAAGTTGCTGCTAATCTCATCAGGCTACTGTCCTATGGCAACAAGAATATGTTGCAAACTGGGTTGATTGTTGGAGGCTGGGACAAGTACGAAGGGGGAAAGATTTATGGAGTTCCTCTTGGCGGCACAGTAATTGAGGCGCCATTTGCCATTGGAGGTTCTGGCTCCAGTTACTTGTATGGATTCTTTGATCAAGCATGGAGAGAAGGAATGACCAAGGACGAAGCGGAGAAGTTGGTTGTGAAGGCAGTTTCCCTAGCCATTGCACGAGATGGAGCCAGTGGTGGTGTTGTCCGCACTGTTGTGATTAATTCTGAGGGAGTTAAAAGAAATTTCTACGCTGGCGACACACTGCCACTGTGGCACGAGGAGTTGGAGCCCCAGAATTCTCTCCTGGACATCTTGGCTGCTGGTGGTGATGGCCCTGCCCCTATGAACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

241

Amino Acids

25.63

Weight (kDa)

5.33

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 17 - 197 1.4e-49 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AccB1I GGYRCC 3 cut(s) 53, 98, 421
AccI GTMKAC 1 cut(s) 177
AciI CCGC 4 cut(s) 84, 405, 509, 577
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 2 cut(s) 613, 667
AcyI GRCGYC 4 cut(s) 69, 87, 99, 422
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 404
AgeI ACCGGT 1 cut(s) 119
AjnI CCWGG 1 cut(s) 675
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 2 cut(s) 163, 262
AluI AGCT 2 cut(s) 163, 262
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 202
AoxI GGCC 1 cut(s) 703
ApeKI GCWGC 4 cut(s) 200, 268, 290, 689
ApoI RAATTY 2 cut(s) 613, 667
AseI ATTAAT 1 cut(s) 591
AsiGI ACCGGT 1 cut(s) 119
AspLEI GCGC 2 cut(s) 101, 424
AspS9I GGNCC 1 cut(s) 704
AsuHPI GGTGA 2 cut(s) 64, 710
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 3 cut(s) 53, 98, 421
BanII GRGCYC 1 cut(s) 663
BauI CACGAG 2 cut(s) 549, 647
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 4 cut(s) 187, 277, 280, 676
BccI CCATC 3 cut(s) 68, 548, 695
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 677
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 263, 539
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 2 cut(s) 102, 425
BglI GCCNNNNNGGC 1 cut(s) 643
BisI GCNGC 6 cut(s) 85, 201, 269, 291, 406, 690
BlsI GCNGC 6 cut(s) 86, 202, 270, 292, 407, 691
Bme1390I CCNGG 1 cut(s) 677
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 6 cut(s) 55, 100, 423, 448, 559, 660
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 433
BsaHI GRCGYC 4 cut(s) 69, 87, 99, 422
BsaJI CCNNGG 3 cut(s) 48, 94, 498
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 404
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 346, 450, 561
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 3 cut(s) 48, 94, 498
BseLI CCNNNNNNNGG 1 cut(s) 404
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 3 cut(s) 173, 224, 588
BseNI ACTGG 3 cut(s) 346, 450, 561
BseRI GAGGAG 1 cut(s) 665
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 4 cut(s) 187, 277, 280, 676
BseYI CCCAGC 1 cut(s) 360
BshFI GGCC 1 cut(s) 705
BshNI GGYRCC 3 cut(s) 53, 98, 421
BshTI ACCGGT 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 404
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 705
Bsp1286I GDGCHC 3 cut(s) 56, 146, 663
Bsp143I GATC 2 cut(s) 194, 472
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 4 cut(s) 84, 405, 509, 577
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 3 cut(s) 172, 223, 589
BspLI GGNNCC 6 cut(s) 55, 100, 423, 448, 559, 660
BspMAI CTGCAG 1 cut(s) 205
BspPI GGATC 1 cut(s) 202
BspT107I GGYRCC 3 cut(s) 53, 98, 421
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 346, 450, 561
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 48, 94, 498
BssMI GATC 2 cut(s) 194, 472
BssNI GRCGYC 4 cut(s) 69, 87, 99, 422
BssSI CACGAG 2 cut(s) 549, 647
BssT1I CCWWGG 2 cut(s) 48, 498
Bst2BI CACGAG 2 cut(s) 549, 647
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 5 cut(s) 280, 312, 412, 583, 642
BstACI GRCGYC 4 cut(s) 69, 87, 99, 422
BstC8I GCNNGC 2 cut(s) 273, 625
BstDEI CTNAG 3 cut(s) 159, 210, 597
BstDSI CCRYGG 1 cut(s) 48
BstH2I RGCGCY 2 cut(s) 102, 425
BstHHI GCGC 2 cut(s) 101, 424
BstKTI GATC 2 cut(s) 197, 475
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 194, 472
BstMWI GCNNNNNNNGC 3 cut(s) 268, 428, 643
BstNI CCWGG 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 56
BstV1I GCAGC 4 cut(s) 187, 277, 280, 676
BsuRI GGCC 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 48
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 4 cut(s) 568, 579, 632, 638
Cac8I GCNNGC 2 cut(s) 273, 625
CfoI GCGC 2 cut(s) 101, 424
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 704
CseI GACGC 2 cut(s) 58, 76
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 1 cut(s) 119
CviAII CATG 2 cut(s) 49, 480
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 159, 210, 597
DinI GGCGCC 2 cut(s) 100, 423
DpnI GATC 2 cut(s) 196, 474
DpnII GATC 2 cut(s) 194, 472
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eco130I CCWWGG 2 cut(s) 48, 498
Eco24I GRGCYC 1 cut(s) 663
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 675
EcoT14I CCWWGG 2 cut(s) 48, 498
EcoT38I GRGCYC 1 cut(s) 663
EgeI GGCGCC 2 cut(s) 100, 423
EheI GGCGCC 2 cut(s) 100, 423
ErhI CCWWGG 2 cut(s) 48, 498
FaeI CATG 2 cut(s) 52, 483
FaqI GGGAC 1 cut(s) 377
FatI CATG 2 cut(s) 48, 479
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 6 cut(s) 85, 201, 269, 291, 406, 690
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 6 cut(s) 85, 201, 269, 291, 406, 690
FspBI CTAG 2 cut(s) 263, 539
GlaI GCGC 2 cut(s) 100, 423
GluI GCNGC 6 cut(s) 85, 201, 269, 291, 406, 690
GsaI CCCAGC 1 cut(s) 364
GsuI CTGGAG 1 cut(s) 433
HaeII RGCGCY 2 cut(s) 102, 425
HaeIII GGCC 1 cut(s) 705
HapII CCGG 1 cut(s) 120
HgaI GACGC 2 cut(s) 58, 76
HhaI GCGC 2 cut(s) 101, 424
Hin1I GRCGYC 4 cut(s) 69, 87, 99, 422
Hin1II CATG 2 cut(s) 52, 483
Hin6I GCGC 2 cut(s) 99, 422
HinP1I GCGC 2 cut(s) 99, 422
HinfI GANTC 4 cut(s) 104, 136, 206, 465
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 64, 710
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 3 cut(s) 148, 223, 598
Hpy188III TCNNGA 2 cut(s) 108, 192
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 2 cut(s) 83, 92
HpyAV CCTTC 3 cut(s) 368, 482, 520
HpyCH4III ACNGT 5 cut(s) 280, 312, 412, 583, 642
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 203, 337, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 268, 428, 643
HpyF3I CTNAG 3 cut(s) 159, 210, 597
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 4 cut(s) 69, 87, 99, 422
Hsp92II CATG 2 cut(s) 52, 483
HspAI GCGC 2 cut(s) 99, 422
KasI GGCGCC 2 cut(s) 98, 421
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 2 cut(s) 194, 472
LmnI GCTCC 3 cut(s) 452, 557, 658
Lsp1109I GCAGC 4 cut(s) 187, 277, 280, 676
MaeI CTAG 2 cut(s) 263, 539
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 70, 452
MalI GATC 2 cut(s) 196, 474
MboI GATC 2 cut(s) 194, 472
MboII GAAGA 1 cut(s) 233
MhlI GDGCHC 3 cut(s) 56, 146, 663
MluCI AATT 4 cut(s) 414, 592, 613, 667
Mly113I GGCGCC 2 cut(s) 99, 422
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 3 cut(s) 246, 334, 636
MseI TTAA 2 cut(s) 591, 606
MslI CAYNNNNRTG 1 cut(s) 584
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 1 cut(s) 677
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 3 cut(s) 268, 428, 643
NarI GGCGCC 2 cut(s) 99, 422
NcoI CCATGG 1 cut(s) 48
NdeII GATC 2 cut(s) 194, 472
NlaIII CATG 2 cut(s) 52, 483
NlaIV GGNNCC 6 cut(s) 55, 100, 423, 448, 559, 660
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 584
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 87
PfeI GAWTC 3 cut(s) 136, 206, 465
PfoI TCCNGGA 1 cut(s) 675
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 6 cut(s) 86, 202, 270, 292, 407, 691
PleI GAGTC 1 cut(s) 98
PluTI GGCGCC 2 cut(s) 102, 425
PpsI GAGTC 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 360
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 6 cut(s) 55, 100, 423, 448, 559, 660
PspPI GGNCC 1 cut(s) 704
PstI CTGCAG 1 cut(s) 205
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 584
SaqAI TTAA 2 cut(s) 591, 606
SatI GCNGC 6 cut(s) 85, 201, 269, 291, 406, 690
Sau3AI GATC 2 cut(s) 194, 472
Sau96I GGNCC 1 cut(s) 704
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 3 cut(s) 56, 146, 663
SetI ASST 8 cut(s) 33, 77, 116, 165, 183, 216, 264, 442
SfcI CTRYAG 1 cut(s) 201
SfoI GGCGCC 2 cut(s) 100, 423
SgrAI CRCCGGYG 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 584
Sse9I AATT 4 cut(s) 414, 592, 613, 667
SsiI CCGC 4 cut(s) 84, 405, 509, 577
SspDI GGCGCC 2 cut(s) 98, 421
SspMI CTAG 2 cut(s) 263, 539
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 2 cut(s) 48, 498
TaaI ACNGT 5 cut(s) 280, 312, 412, 583, 642
TaiI ACGT 1 cut(s) 183
TasI AATT 4 cut(s) 414, 592, 613, 667
TauI GCSGC 2 cut(s) 87, 408
TfiI GAWTC 3 cut(s) 136, 206, 465
Tru1I TTAA 2 cut(s) 591, 606
Tru9I TTAA 2 cut(s) 591, 606
TscAI CASTG 4 cut(s) 568, 586, 639, 645
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 4 cut(s) 200, 268, 290, 689
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 2 cut(s) 41, 233
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 4 cut(s) 568, 586, 639, 645
VspI ATTAAT 1 cut(s) 591
XapI RAATTY 2 cut(s) 613, 667
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 2 cut(s) 263, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.