Rh5CG280300

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003666
Physical Location & Seq
Forward (+)
30578743 .. 30583939
5197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG280300.1

Sequence Viewer

Length: 441 bp
ATGATCAGCTTTGTGATGACCGCGATTGCGCCATCACACACTGGTAGCACAGCGCCACCGTCCGTCATAGGAGTCTTTGATTCGAAGCACTTAAACCCTGGTATCTATGTTGCGAATCGGTGCTCGGATAAAATCACTCAGCTCACGGATAAGGTCTACGTCTGTCGCTCTGGATCGGCTGCAGATTCTCAGGTTGTTTCCGACTATGTGATGCACTTTCTTCATCAGCACACCATTCAGCTATGGCAGCCTGCTACTGTCAAAGTTGTTGCTAATAATCTCATCAGGCTACTGTCATATGGCAACAAGAATATGTTGCAAACTGGGTTGATTGTTGGAGGCTGGGACAAGTATGAAGGGGGAAAGATTTATGGAGTTCTGCTTGGCGGCACAGTCATAAAGGCGCCATTTGCCATTGGAGCTAAAGATTTATACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

146

Amino Acids

15.78

Weight (kDa)

9.12

Isoelectric Point (pI)

26.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 34 - 143 6.2e-20 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 156
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 21, 387
AclWI GGATC 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 4 cut(s) 9, 142, 241, 422
AluI AGCT 4 cut(s) 9, 142, 241, 422
Alw21I GWGCWC 1 cut(s) 125
AlwI GGATC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 179, 247
AspLEI GCGC 3 cut(s) 31, 55, 406
AsuII TTCGAA 1 cut(s) 83
BaeI ACNNNNGTAYC 2 cut(s) 85, 118
BanI GGYRCC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 2 cut(s) 166, 259
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 99
BclI TGATCA 1 cut(s) 3
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 2 cut(s) 56, 407
BisI GCNGC 3 cut(s) 180, 248, 388
BlsI GCNGC 3 cut(s) 181, 249, 389
Bme1390I CCNGG 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 99
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 46, 328
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 152, 203
BseNI ACTGG 2 cut(s) 46, 328
BseXI GCAGC 2 cut(s) 166, 259
BseYI CCCAGC 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 23
BshNI GGYRCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 125
BslFI GGGAC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 3, 173
BspACI CCGC 2 cut(s) 21, 387
BspCNI CTCAG 2 cut(s) 151, 202
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 83
BspT107I GGYRCC 1 cut(s) 403
BsrI ACTGG 2 cut(s) 46, 328
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 3, 173
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 60, 259, 294, 394
BstACI GRCGYC 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 138, 189
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 56, 407
BstHHI GCGC 3 cut(s) 31, 55, 406
BstKTI GATC 2 cut(s) 6, 176
BstMBI GATC 2 cut(s) 3, 173
BstMWI GCNNNNNNNGC 3 cut(s) 247, 410, 419
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 97
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 2 cut(s) 166, 259
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 3 cut(s) 31, 55, 406
CviJI RGCY 8 cut(s) 9, 142, 179, 241, 250, 289, 342, 422
CviKI_1 RGCY 8 cut(s) 9, 142, 179, 241, 250, 289, 342, 422
DdeI CTNAG 2 cut(s) 138, 189
DinI GGCGCC 1 cut(s) 405
DpnI GATC 2 cut(s) 5, 175
DpnII GATC 2 cut(s) 3, 173
EcoRII CCWGG 1 cut(s) 97
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
FaqI GGGAC 1 cut(s) 359
FauNDI CATATG 1 cut(s) 298
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 180, 248, 388
Fsp4HI GCNGC 3 cut(s) 180, 248, 388
GlaI GCGC 3 cut(s) 30, 54, 405
GluI GCNGC 3 cut(s) 180, 248, 388
GsaI CCCAGC 1 cut(s) 346
HaeII RGCGCY 2 cut(s) 56, 407
HhaI GCGC 3 cut(s) 31, 55, 406
Hin1I GRCGYC 1 cut(s) 404
Hin6I GCGC 3 cut(s) 29, 53, 404
HinP1I GCGC 3 cut(s) 29, 53, 404
HinfI GANTC 4 cut(s) 72, 80, 115, 185
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 2 cut(s) 127, 202
Hpy188III TCNNGA 1 cut(s) 171
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 4 cut(s) 60, 259, 294, 394
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 182, 214, 319
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 410, 419
HpyF3I CTNAG 2 cut(s) 138, 189
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 404
HspAI GCGC 3 cut(s) 29, 53, 404
KasI GGCGCC 1 cut(s) 403
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 173
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 9 cut(s) 27, 84, 111, 156, 176, 264, 271, 309, 328
Lsp1109I GCAGC 2 cut(s) 166, 259
LweI GCATC 1 cut(s) 201
MaeII ACGT 1 cut(s) 159
MalI GATC 2 cut(s) 5, 175
MboI GATC 2 cut(s) 3, 173
MboII GAAGA 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 125
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 2 cut(s) 225, 316
MnlI CCTC 1 cut(s) 332
MseI TTAA 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 99
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 247, 410, 419
NarI GGCGCC 1 cut(s) 404
NdeI CATATG 1 cut(s) 298
NdeII GATC 2 cut(s) 3, 173
NlaIV GGNNCC 1 cut(s) 405
NspV TTCGAA 1 cut(s) 83
PfeI GAWTC 3 cut(s) 80, 115, 185
PkrI GCNGC 3 cut(s) 181, 249, 389
PleI GAGTC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 342
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 405
PstI CTGCAG 1 cut(s) 184
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 3 cut(s) 180, 248, 388
Sau3AI GATC 2 cut(s) 3, 173
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 125
SetI ASST 7 cut(s) 11, 144, 156, 162, 195, 243, 424
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 180
SfoI GGCGCC 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 83
SsiI CCGC 2 cut(s) 21, 387
SspDI GGCGCC 1 cut(s) 403
StyD4I CCNGG 1 cut(s) 97
TaaI ACNGT 4 cut(s) 60, 259, 294, 394
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 83
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 3 cut(s) 80, 115, 185
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TscAI CASTG 1 cut(s) 46
TseI GCWGC 2 cut(s) 179, 247
TspDTI ATGAA 2 cut(s) 212, 369
TspGWI ACGGA 2 cut(s) 52, 161
TspRI CASTG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.