MD16G1239000.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003666
Physical Location & Seq
Forward (+)
25272296 .. 25277597
5302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1239000.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGATCAGTCCATACAGAATGACCTTAATGCCCCTCACTCCATGGGCACCACCATTATCGGCGTCACCTACAATGGCGGCGTCGTCCTCGGCGCCGATTCCCGAACCAGCACCGGTGTTTATGTTGCGAACCGAGCATCCGATAAGATCACTCAGCTCACTGACAATGTCTACTTGTGTCGCTCCGGATCGGCTGCTGATTCTCAAATTGTGTCTGATTACGTGCGTCACTTTCTTCATCAGCACACGATACAGCTGGGCCAGCCTGCAACAGTCAAGGTTGCTGCAAACCTGATCAGGTTGTTCTCATACAATAACAAGAATATGCTACAAACTGGCTTGATTATTGGTGGTTGGGACAAGTATGAAGGAGGTAAAATATATGGAGTGCCACTCGGAGGGACAATTTTAGAGCAGCCTTTTGCTATTGGAGGATCCGGCTCCACTTATCTGTATGGTTTCTTTGATCAAGAGTGGAAGGAAGGAATGACCAAAGAGGAAGCTGAGCAATTGGTGGTGAAAGCAGTTTCCCTTGCTATTGCTCGAGATGGTGCTAGTGGTGGTGTCGTTCGAACTGTCATTATAAATTCAGAACGGGTGACAAGAAACTATTATCCCGGAGACGCCCTTCCGCTCTGGCACGAGGAGCTGGAACCCCAGAATTCTATGCTGGATATATTGTCATCATCTTCATCTGGTCCAGACATGATGATAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

240

Amino Acids

25.87

Weight (kDa)

5.17

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 15 - 195 5.6e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AccB1I GGYRCC 2 cut(s) 47, 92
AccBSI CCGCTC 1 cut(s) 634
AccI GTMKAC 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 2 cut(s) 78, 632
AclWI GGATC 4 cut(s) 12, 196, 429, 442
AcsI RAATTY 2 cut(s) 586, 661
AcyI GRCGYC 4 cut(s) 63, 81, 93, 624
AfiI CCNNNNNNNGG 1 cut(s) 398
AgeI ACCGGT 1 cut(s) 113
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 4 cut(s) 157, 256, 503, 649
AluI AGCT 4 cut(s) 157, 256, 503, 649
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 4 cut(s) 12, 196, 429, 442
Ama87I CYCGRG 1 cut(s) 543
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 3 cut(s) 194, 284, 415
ApoI RAATTY 2 cut(s) 586, 661
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 259, 698
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 3 cut(s) 58, 529, 610
AsuII TTCGAA 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 50
BamHI GGATCC 1 cut(s) 434
BanI GGYRCC 2 cut(s) 47, 92
BauI CACGAG 1 cut(s) 641
BbvI GCAGC 3 cut(s) 181, 271, 427
BccI CCATC 1 cut(s) 542
BclI TGATCA 2 cut(s) 294, 466
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 555
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 4 cut(s) 79, 195, 285, 416
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 4 cut(s) 80, 196, 286, 417
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 2 cut(s) 259, 698
BmiI GGNNCC 5 cut(s) 49, 94, 436, 442, 654
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 1 cut(s) 146
BplI GAGNNNNNCTC 2 cut(s) 378, 410
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 223
BsaHI GRCGYC 4 cut(s) 63, 81, 93, 624
BsaJI CCNNGG 2 cut(s) 42, 88
BsaWI WCCGGW 2 cut(s) 113, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 42, 88
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 167, 495
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 3 cut(s) 181, 271, 427
BseYI CCCAGC 1 cut(s) 256
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 2 cut(s) 47, 92
BshTI ACCGGT 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 4 cut(s) 114, 186, 438, 618
BslFI GGGAC 2 cut(s) 371, 415
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 615
BsmBI CGTCTC 1 cut(s) 615
BsmFI GGGAC 2 cut(s) 371, 415
BsnI GGCC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 543
Bsp119I TTCGAA 1 cut(s) 571
Bsp1286I GDGCHC 1 cut(s) 50
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 6 cut(s) 4, 147, 188, 294, 434, 466
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 78, 632
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 166, 496
BspEI TCCGGA 1 cut(s) 185
BspLI GGNNCC 5 cut(s) 49, 94, 436, 442, 654
BspPI GGATC 4 cut(s) 12, 196, 429, 442
BspT104I TTCGAA 1 cut(s) 571
BspT107I GGYRCC 2 cut(s) 47, 92
BsrBI CCGCTC 1 cut(s) 634
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 42, 88
BssMI GATC 6 cut(s) 4, 147, 188, 294, 434, 466
BssNI GRCGYC 4 cut(s) 63, 81, 93, 624
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 641
Bst4CI ACNGT 2 cut(s) 274, 577
BstACI GRCGYC 4 cut(s) 63, 81, 93, 624
BstBAI YACGTR 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 263, 267
BstDEI CTNAG 2 cut(s) 153, 504
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 6 cut(s) 7, 150, 191, 297, 437, 469
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 6 cut(s) 4, 147, 188, 294, 434, 466
BstMWI GCNNNNNNNGC 3 cut(s) 134, 262, 646
BstSCI CCNGG 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 3 cut(s) 181, 271, 427
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 2 cut(s) 263, 267
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 259, 698
CseI GACGC 4 cut(s) 52, 70, 215, 632
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 2 cut(s) 43, 706
DdeI CTNAG 2 cut(s) 153, 504
DinI GGCGCC 1 cut(s) 94
DpnI GATC 6 cut(s) 6, 149, 190, 296, 436, 468
DpnII GATC 6 cut(s) 4, 147, 188, 294, 434, 466
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 42
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 615
FaeI CATG 2 cut(s) 46, 709
FaqI GGGAC 2 cut(s) 371, 415
FatI CATG 2 cut(s) 42, 705
FbaI TGATCA 2 cut(s) 294, 466
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 79, 195, 285, 416
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 4 cut(s) 79, 195, 285, 416
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 94
GluI GCNGC 4 cut(s) 79, 195, 285, 416
GsaI CCCAGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 261
HapII CCGG 4 cut(s) 114, 186, 438, 618
HgaI GACGC 4 cut(s) 52, 70, 215, 632
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 4 cut(s) 63, 81, 93, 624
Hin1II CATG 2 cut(s) 46, 709
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 2 cut(s) 98, 200
HpaII CCGG 4 cut(s) 114, 186, 438, 618
HphI GGTGA 3 cut(s) 58, 529, 610
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 142, 217, 398, 592
Hpy188III TCNNGA 5 cut(s) 102, 186, 470, 545, 701
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 4 cut(s) 362, 472, 476, 638
HpyCH4III ACNGT 2 cut(s) 274, 577
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 269, 287
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 262, 646
HpyF3I CTNAG 2 cut(s) 153, 504
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 4 cut(s) 63, 81, 93, 624
Hsp92II CATG 2 cut(s) 46, 709
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 185
Ksp22I TGATCA 2 cut(s) 294, 466
Kzo9I GATC 6 cut(s) 4, 147, 188, 294, 434, 466
LmnI GCTCC 3 cut(s) 188, 446, 646
Lsp1109I GCAGC 3 cut(s) 181, 271, 427
LweI GCATC 1 cut(s) 146
MaeI CTAG 1 cut(s) 555
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 64, 227, 598
MalI GATC 6 cut(s) 6, 149, 190, 296, 436, 468
MbiI CCGCTC 1 cut(s) 634
MboI GATC 6 cut(s) 4, 147, 188, 294, 434, 466
MboII GAAGA 2 cut(s) 227, 681
MfeI CAATTG 1 cut(s) 509
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 5 cut(s) 207, 405, 509, 586, 661
Mly113I GGCGCC 1 cut(s) 93
MnlI CCTC 7 cut(s) 45, 98, 365, 392, 425, 490, 637
MroI TCCGGA 1 cut(s) 185
MseI TTAA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 4 cut(s) 114, 186, 438, 618
MspR9I CCNGG 1 cut(s) 618
MunI CAATTG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 134, 262, 646
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 1 cut(s) 42
NdeII GATC 6 cut(s) 4, 147, 188, 294, 434, 466
NlaIII CATG 2 cut(s) 46, 709
NlaIV GGNNCC 5 cut(s) 49, 94, 436, 442, 654
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 3 cut(s) 64, 227, 598
NspV TTCGAA 1 cut(s) 571
PaeR7I CTCGAG 1 cut(s) 543
PfeI GAWTC 2 cut(s) 98, 200
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 616
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 80, 196, 286, 417
PluTI GGCGCC 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 223
PsiI TTATAA 1 cut(s) 584
PspFI CCCAGC 1 cut(s) 256
PspN4I GGNNCC 5 cut(s) 49, 94, 436, 442, 654
PspPI GGNCC 2 cut(s) 259, 698
PsuI RGATCY 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 256
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 4 cut(s) 79, 195, 285, 416
Sau3AI GATC 6 cut(s) 4, 147, 188, 294, 434, 466
Sau96I GGNCC 2 cut(s) 259, 698
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 1 cut(s) 50
SfaNI GCATC 1 cut(s) 146
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 571
SgrAI CRCCGGYG 1 cut(s) 113
SinI GGWCC 1 cut(s) 698
SlaI CTCGAG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 5 cut(s) 207, 405, 509, 586, 661
SsiI CCGC 2 cut(s) 78, 632
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 274, 577
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 544, 571
TasI AATT 5 cut(s) 207, 405, 509, 586, 661
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 98, 200
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 3 cut(s) 64, 227, 598
TseI GCWGC 3 cut(s) 194, 284, 415
Tsp45I GTSAC 3 cut(s) 64, 227, 598
TspDTI ATGAA 3 cut(s) 227, 381, 681
TspRI CASTG 1 cut(s) 166
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 698
XapI RAATTY 2 cut(s) 586, 661
XhoI CTCGAG 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.