RLG00000008279

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003666
Physical Location & Seq
Reverse (-)
28208621 .. 28210354
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008279

Sequence Viewer

Length: 516 bp
ATGTTACAAATTGGGTTGATTGTTGGAGGCTGGGACAAGTATGAAGGGGGAAAGATTTATGGAGTTCCGCTTGGAGGCACAGTCATAGAGGCGCCATTTGCCATTGGAGGTTCTGAATCCAGTTACTTGTATGGATTCTTTGATCAAGCATGGAGAGAAGGAATGACCAAGGACGAAGCGGAGCTCTTTTGTGCCTTATTGCAAAAGTTGGTTGTGAAGGCAGTTTCCCTAGCCATTGCACGAGATGGAGCCAGTGGTGGTGTTGTCCGCATTGTTGTGATGGAACTATGTCCAAATGTGAATCCCAACTCAGTTGCTTACATTTCTGACAGTACATGTGGGCACTTGAGCGGCTCAACTGACAGTGACACTAATGACGCAAAGAAAATTGGTGCTGAAGAAGATTTTGAGAATTTTCTTGGTTGCTTCAAAGATACGAATATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGTGGTGTGCACAAGGTATTTTTACTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

172

Amino Acids

18.34

Weight (kDa)

4.88

Isoelectric Point (pI)

22.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 93 2.7e-15 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 457
AccBSI CCGCTC 1 cut(s) 351
AciI CCGC 4 cut(s) 68, 179, 268, 351
AclWI GGATC 2 cut(s) 455, 468
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 334
AfiI CCNNNNNNNGG 3 cut(s) 74, 457, 470
AflIII ACRYGT 1 cut(s) 335
AgsI TTSAA 1 cut(s) 430
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw21I GWGCWC 2 cut(s) 186, 495
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 2 cut(s) 455, 468
ApaLI GTGCAC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 412
AspLEI GCGC 1 cut(s) 94
BaeGI GKGCMC 2 cut(s) 345, 495
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 186
BauI CACGAG 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 186, 495
BccI CCATC 2 cut(s) 239, 274
BclI TGATCA 1 cut(s) 142
BfaI CTAG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 1 cut(s) 352
BlsI GCNGC 1 cut(s) 353
BmiI GGNNCC 3 cut(s) 93, 250, 462
BpuEI CTTGAG 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 168, 451
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 457, 470
Bse1I ACTGG 2 cut(s) 120, 252
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 168, 451
BseLI CCNNNNNNNGG 3 cut(s) 74, 457, 470
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 120, 252
BseSI GKGCMC 2 cut(s) 345, 495
BseYI CCCAGC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 186, 495
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 3 cut(s) 74, 457, 470
BsmFI GGGAC 1 cut(s) 47
Bsp1286I GDGCHC 3 cut(s) 186, 345, 495
Bsp143I GATC 2 cut(s) 142, 460
BspACI CCGC 4 cut(s) 68, 179, 268, 351
BspCNI CTCAG 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 93, 250, 462
BspPI GGATC 2 cut(s) 455, 468
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 2 cut(s) 120, 252
BssECI CCNNGG 2 cut(s) 168, 451
BssMI GATC 2 cut(s) 142, 460
BssNI GRCGYC 1 cut(s) 92
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 168, 451
Bst2BI CACGAG 1 cut(s) 240
Bst4CI ACNGT 3 cut(s) 82, 332, 365
BstACI GRCGYC 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 2 cut(s) 310, 509
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 2 cut(s) 145, 463
BstMBI GATC 2 cut(s) 142, 460
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 339
BstSLI GKGCMC 2 cut(s) 345, 495
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 259, 370
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 94
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 333
CviAII CATG 2 cut(s) 150, 336
CviJI RGCY 6 cut(s) 30, 184, 233, 251, 354, 449
CviKI_1 RGCY 6 cut(s) 30, 184, 233, 251, 354, 449
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 2 cut(s) 310, 509
DinI GGCGCC 1 cut(s) 93
DpnI GATC 2 cut(s) 144, 462
DpnII GATC 2 cut(s) 142, 460
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 168, 451
Eco24I GRGCYC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 168, 451
EcoT38I GRGCYC 1 cut(s) 186
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 2 cut(s) 168, 451
FaeI CATG 2 cut(s) 153, 339
FaiI YATR 8 cut(s) 42, 60, 86, 132, 151, 289, 337, 443
FaqI GGGAC 1 cut(s) 47
FatI CATG 2 cut(s) 149, 335
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 352
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 352
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 352
GsaI CCCAGC 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 95
HgaI GACGC 1 cut(s) 386
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 2 cut(s) 153, 339
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 3 cut(s) 116, 135, 301
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 2 cut(s) 115, 328
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 3 cut(s) 38, 152, 211
HpyCH4III ACNGT 3 cut(s) 82, 332, 365
HpyCH4V TGCA 4 cut(s) 202, 239, 445, 493
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 310, 509
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 2 cut(s) 153, 339
HspAI GCGC 1 cut(s) 92
KasI GGCGCC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 2 cut(s) 142, 460
LmnI GCTCC 2 cut(s) 181, 248
LpnPI CCDG 3 cut(s) 16, 133, 265
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 3 cut(s) 3, 122, 365
MalI GATC 2 cut(s) 144, 462
MbiI CCGCTC 1 cut(s) 351
MboI GATC 2 cut(s) 142, 460
MboII GAAGA 2 cut(s) 410, 413
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 3 cut(s) 186, 345, 495
MluCI AATT 3 cut(s) 9, 387, 412
Mly113I GGCGCC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 4
MnlI CCTC 5 cut(s) 20, 68, 82, 101, 462
MslI CAYNNNNRTG 1 cut(s) 275
MwoI GCNNNNNNNGC 1 cut(s) 98
NarI GGCGCC 1 cut(s) 92
NdeII GATC 2 cut(s) 142, 460
NlaIII CATG 2 cut(s) 153, 339
NlaIV GGNNCC 3 cut(s) 93, 250, 462
NmuCI GTSAC 1 cut(s) 365
NspI RCATGY 1 cut(s) 339
PciI ACATGT 1 cut(s) 335
PfeI GAWTC 3 cut(s) 116, 135, 301
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 1 cut(s) 353
PluTI GGCGCC 1 cut(s) 95
PscI ACATGT 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 30
PspN4I GGNNCC 3 cut(s) 93, 250, 462
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 186
SatI GCNGC 1 cut(s) 352
Sau3AI GATC 2 cut(s) 142, 460
SduI GDGCHC 3 cut(s) 186, 345, 495
SetI ASST 4 cut(s) 112, 186, 457, 501
SfoI GGCGCC 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 3 cut(s) 9, 387, 412
SsiI CCGC 4 cut(s) 68, 179, 268, 351
SspDI GGCGCC 1 cut(s) 91
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 186
StyI CCWWGG 2 cut(s) 168, 451
TaaI ACNGT 3 cut(s) 82, 332, 365
TasI AATT 3 cut(s) 9, 387, 412
TatI WGTACW 1 cut(s) 332
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 3 cut(s) 116, 135, 301
TscAI CASTG 2 cut(s) 259, 370
TseFI GTSAC 1 cut(s) 365
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 2 cut(s) 259, 370
Van91I CCANNNNNTGG 1 cut(s) 457
VneI GTGCAC 1 cut(s) 491
XapI RAATTY 1 cut(s) 412
XceI RCATGY 1 cut(s) 339
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.