MD13G1234400.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003666
Physical Location & Seq
Forward (+)
23423306 .. 23430602
7297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1234400.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGATCAGTCGATACAGAATGACCTTAATGCCCCTCACTCCATGGGCACCACCATCATCGGCGTCACCTACAATGGCGGCGTCGTCCTCGGCGCCGATTCTCGTACCAGCACTGGTGTTTATGTTGCAAATCGAGCATCCGACAAGATCACTCAGCTTACTGATAATGTCTACTTGTGCCGCTCCGGATCGGCTGCAGATTCTCAAATTGTGTCTGATTACGTGCGTCACTTTCTCCATCAGCACACGATACAGCTGGGCCAGCCTGCAACAGTCAAGGTTGCCGCAAACCTTATCAGGTTGTTCTCATACAATAACAAGAATATGCTACAAACTGGCATGATTATTGGTGGTTGGGACAAGTATGAAGGAGGTAAAATATATGGAGTACCACTCGGAGGGACAATTTTAGAGCAGCCTTTTGCTATTGGAGGATCTGGCTCCACTTATCTGTATGGTTTCTTTGATCAAGAGTGGAAGGAAGGAATGACCAAAGAGGAAGCTGAGCAATTGGTGGTGAAAGCAGTTTCCCTCGCTATTGCTCGAGATGGTGCTAGTGGTGGTGTCGTTCGAACTGTCATTATAAATTCAGAGGGGGTGACGAGAAACTATTATCCAGGTGACACCCTTCCGCTCTGGCATGAGGAGCTCGAACCCCAGAATTCTTTGCTGGATATCTTGTCATCATCATCATCTGGTCCGGACATGATGATAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

240

Amino Acids

25.8

Weight (kDa)

5.04

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 15 - 195 6.5e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AccB1I GGYRCC 2 cut(s) 47, 92
AccBSI CCGCTC 2 cut(s) 183, 634
AccI GTMKAC 1 cut(s) 171
AccIII TCCGGA 2 cut(s) 185, 700
AciI CCGC 4 cut(s) 78, 181, 285, 632
AclWI GGATC 3 cut(s) 12, 196, 442
AcsI RAATTY 2 cut(s) 586, 661
AcyI GRCGYC 3 cut(s) 63, 81, 93
AfaI GTAC 2 cut(s) 106, 390
AfiI CCNNNNNNNGG 1 cut(s) 398
AjnI CCWGG 1 cut(s) 616
AluBI AGCT 4 cut(s) 157, 256, 503, 649
AluI AGCT 4 cut(s) 157, 256, 503, 649
Alw21I GWGCWC 1 cut(s) 651
AlwI GGATC 3 cut(s) 12, 196, 442
Ama87I CYCGRG 1 cut(s) 543
Aor13HI TCCGGA 2 cut(s) 185, 700
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 2 cut(s) 194, 415
ApoI RAATTY 2 cut(s) 586, 661
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 259, 698
AsuHPI GGTGA 4 cut(s) 58, 529, 610, 632
AsuII TTCGAA 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 50
BanI GGYRCC 2 cut(s) 47, 92
BanII GRGCYC 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 181, 427
BccI CCATC 3 cut(s) 62, 246, 542
BciT130I CCWGG 1 cut(s) 618
BclI TGATCA 1 cut(s) 466
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 5 cut(s) 79, 181, 195, 285, 416
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 5 cut(s) 80, 182, 196, 286, 417
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 2 cut(s) 259, 698
BmiI GGNNCC 3 cut(s) 49, 94, 442
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 1 cut(s) 146
BplI GAGNNNNNCTC 2 cut(s) 378, 410
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 571
BsaAI YACGTR 1 cut(s) 223
BsaHI GRCGYC 3 cut(s) 63, 81, 93
BsaJI CCNNGG 2 cut(s) 42, 88
BsaWI WCCGGW 2 cut(s) 185, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 118, 340
BseAI TCCGGA 2 cut(s) 185, 700
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 2 cut(s) 42, 88
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 167, 495
BseNI ACTGG 2 cut(s) 118, 340
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 2 cut(s) 181, 427
BseYI CCCAGC 1 cut(s) 256
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 2 cut(s) 47, 92
BsiHKAI GWGCWC 1 cut(s) 651
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 2 cut(s) 186, 701
BslFI GGGAC 2 cut(s) 371, 415
BslI CCNNNNNNNGG 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 371, 415
BsnI GGCC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 543
Bsp119I TTCGAA 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 50, 651
Bsp13I TCCGGA 2 cut(s) 185, 700
Bsp143I GATC 5 cut(s) 4, 147, 188, 434, 466
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 4 cut(s) 78, 181, 285, 632
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 166, 496
BspEI TCCGGA 2 cut(s) 185, 700
BspLI GGNNCC 3 cut(s) 49, 94, 442
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 3 cut(s) 12, 196, 442
BspT104I TTCGAA 1 cut(s) 571
BspT107I GGYRCC 2 cut(s) 47, 92
BsrBI CCGCTC 2 cut(s) 183, 634
BsrI ACTGG 2 cut(s) 118, 340
BssECI CCNNGG 2 cut(s) 42, 88
BssMI GATC 5 cut(s) 4, 147, 188, 434, 466
BssNI GRCGYC 3 cut(s) 63, 81, 93
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 2 cut(s) 274, 577
BstACI GRCGYC 3 cut(s) 63, 81, 93
BstBAI YACGTR 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 263, 267
BstDEI CTNAG 2 cut(s) 153, 504
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 7, 150, 191, 437, 469
BstMBI GATC 5 cut(s) 4, 147, 188, 434, 466
BstMWI GCNNNNNNNGC 3 cut(s) 134, 262, 646
BstNI CCWGG 1 cut(s) 618
BstSCI CCNGG 1 cut(s) 616
BstSFI CTRYAG 1 cut(s) 195
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 181, 427
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 263, 267
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 2 cut(s) 259, 698
CseI GACGC 3 cut(s) 52, 70, 215
Csp6I GTAC 2 cut(s) 105, 389
CviAII CATG 4 cut(s) 43, 340, 641, 706
CviJI RGCY 9 cut(s) 157, 194, 256, 261, 265, 418, 441, 503, 649
CviKI_1 RGCY 9 cut(s) 157, 194, 256, 261, 265, 418, 441, 503, 649
CviQI GTAC 2 cut(s) 105, 389
DdeI CTNAG 2 cut(s) 153, 504
DinI GGCGCC 1 cut(s) 94
DpnI GATC 5 cut(s) 6, 149, 190, 436, 468
DpnII GATC 5 cut(s) 4, 147, 188, 434, 466
Ecl136II GAGCTC 1 cut(s) 649
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 651
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 698
Eco53kI GAGCTC 1 cut(s) 649
Eco88I CYCGRG 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 649
EcoRI GAATTC 1 cut(s) 661
EcoRII CCWGG 1 cut(s) 616
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 651
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 4 cut(s) 46, 343, 644, 709
FaqI GGGAC 2 cut(s) 371, 415
FatI CATG 4 cut(s) 42, 339, 640, 705
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 79, 181, 195, 285, 416
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 5 cut(s) 79, 181, 195, 285, 416
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 94
GluI GCNGC 5 cut(s) 79, 181, 195, 285, 416
GsaI CCCAGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 186, 701
HgaI GACGC 3 cut(s) 52, 70, 215
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 3 cut(s) 63, 81, 93
Hin1II CATG 4 cut(s) 46, 343, 644, 709
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 2 cut(s) 98, 200
HpaII CCGG 2 cut(s) 186, 701
HphI GGTGA 4 cut(s) 58, 529, 610, 632
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 142, 217, 398, 592
Hpy188III TCNNGA 4 cut(s) 186, 470, 545, 701
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 4 cut(s) 362, 472, 476, 638
HpyCH4III ACNGT 2 cut(s) 274, 577
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 3 cut(s) 128, 197, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 262, 646
HpyF3I CTNAG 2 cut(s) 153, 504
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 3 cut(s) 63, 81, 93
Hsp92II CATG 4 cut(s) 46, 343, 644, 709
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 2 cut(s) 185, 700
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 5 cut(s) 4, 147, 188, 434, 466
LmnI GCTCC 3 cut(s) 188, 446, 646
Lsp1109I GCAGC 2 cut(s) 181, 427
LweI GCATC 1 cut(s) 146
MaeI CTAG 1 cut(s) 555
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 4 cut(s) 64, 227, 598, 620
MalI GATC 5 cut(s) 6, 149, 190, 436, 468
MbiI CCGCTC 2 cut(s) 183, 634
MboI GATC 5 cut(s) 4, 147, 188, 434, 466
MfeI CAATTG 1 cut(s) 509
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 2 cut(s) 50, 651
MluCI AATT 5 cut(s) 207, 405, 509, 586, 661
Mly113I GGCGCC 1 cut(s) 93
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 9 cut(s) 45, 98, 365, 392, 425, 490, 542, 586, 637
MroI TCCGGA 2 cut(s) 185, 700
MseI TTAA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 2 cut(s) 186, 701
MspR9I CCNGG 1 cut(s) 618
MunI CAATTG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 134, 262, 646
NarI GGCGCC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 42
NdeII GATC 5 cut(s) 4, 147, 188, 434, 466
NlaIII CATG 4 cut(s) 46, 343, 644, 709
NlaIV GGNNCC 3 cut(s) 49, 94, 442
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 4 cut(s) 64, 227, 598, 620
NspV TTCGAA 1 cut(s) 571
PaeR7I CTCGAG 1 cut(s) 543
PfeI GAWTC 2 cut(s) 98, 200
PkrI GCNGC 5 cut(s) 80, 182, 196, 286, 417
PluTI GGCGCC 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 223
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 3 cut(s) 49, 94, 442
PspPI GGNCC 2 cut(s) 259, 698
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 2 cut(s) 106, 390
RsaNI GTAC 2 cut(s) 105, 389
SacI GAGCTC 1 cut(s) 651
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 5 cut(s) 79, 181, 195, 285, 416
Sau3AI GATC 5 cut(s) 4, 147, 188, 434, 466
Sau96I GGNCC 2 cut(s) 259, 698
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 2 cut(s) 50, 651
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 1 cut(s) 195
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 571
SinI GGWCC 1 cut(s) 698
SlaI CTCGAG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 5 cut(s) 207, 405, 509, 586, 661
SsiI CCGC 4 cut(s) 78, 181, 285, 632
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 274, 577
TaiI ACGT 1 cut(s) 225
TaqI TCGA 5 cut(s) 11, 133, 544, 571, 651
TasI AATT 5 cut(s) 207, 405, 509, 586, 661
TauI GCSGC 3 cut(s) 81, 183, 287
TfiI GAWTC 2 cut(s) 98, 200
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 4 cut(s) 64, 227, 598, 620
TseI GCWGC 2 cut(s) 194, 415
Tsp45I GTSAC 4 cut(s) 64, 227, 598, 620
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 698
XapI RAATTY 2 cut(s) 586, 661
XhoI CTCGAG 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.