RchiOBHm_Chr2g0148551

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003666
Physical Location & Seq
Reverse (-)
66293999 .. 66294944
946 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51811

Sequence Viewer

Length: 270 bp
ATGGATAATGTCTACGTCTATCACTCTGGATCGGCTACAGATTCTCAGGTTGTTTCCGACTATGTGAGGCACTTTCTTCATCAGCACACGATTCAGCTATGGCAGCCTGCTACTGTCAAAGTTGTTGCTAATCTCATCAGGCTACTGTCATATGGCGGCACAGTCATAAAAGCGCCATTTGCCATTGGAGGTTCTGGCTCCAGTTACTTGTATGGATTCTTTGATCAAGCATGGAGAGAAGGAATGACCAAGGACGAAGCGGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

89

Amino Acids

9.93

Weight (kDa)

6.0

Isoelectric Point (pI)

25.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 49 - 88 2e-06 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 12
AciI CCGC 2 cut(s) 156, 260
AclWI GGATC 1 cut(s) 37
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
AlwI GGATC 1 cut(s) 37
ApeKI GCWGC 1 cut(s) 103
AspLEI GCGC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 115
BclI TGATCA 1 cut(s) 223
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 176
BisI GCNGC 2 cut(s) 104, 157
BlsI GCNGC 2 cut(s) 105, 158
BmiI GGNNCC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 249
Bse1I ACTGG 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 29, 223
BspACI CCGC 2 cut(s) 156, 260
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 29, 223
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 115, 147, 163
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 32, 226
BstMBI GATC 2 cut(s) 29, 223
BstMWI GCNNNNNNNGC 2 cut(s) 103, 179
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 175
CviAII CATG 1 cut(s) 231
CviJI RGCY 5 cut(s) 35, 97, 106, 142, 198
CviKI_1 RGCY 5 cut(s) 35, 97, 106, 142, 198
DdeI CTNAG 1 cut(s) 45
DpnI GATC 2 cut(s) 31, 225
DpnII GATC 2 cut(s) 29, 223
Eco130I CCWWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 234
FaiI YATR 8 cut(s) 63, 100, 151, 153, 167, 213, 232, 268
FatI CATG 1 cut(s) 230
FauNDI CATATG 1 cut(s) 151
FbaI TGATCA 1 cut(s) 223
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 104, 157
Fsp4HI GCNGC 2 cut(s) 104, 157
GlaI GCGC 1 cut(s) 174
GluI GCNGC 2 cut(s) 104, 157
GsuI CTGGAG 1 cut(s) 184
HaeII RGCGCY 1 cut(s) 176
HhaI GCGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 234
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 3 cut(s) 41, 91, 216
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 1 cut(s) 27
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 3 cut(s) 115, 147, 163
HpyCH4IV ACGT 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 179
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 1 cut(s) 234
HspAI GCGC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 2 cut(s) 29, 223
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 6 cut(s) 12, 32, 120, 124, 180, 214
Lsp1109I GCAGC 1 cut(s) 115
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 203
MalI GATC 2 cut(s) 31, 225
MboI GATC 2 cut(s) 29, 223
MboII GAAGA 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 3 cut(s) 60, 182, 256
MwoI GCNNNNNNNGC 2 cut(s) 103, 179
NdeI CATATG 1 cut(s) 151
NdeII GATC 2 cut(s) 29, 223
NlaIII CATG 1 cut(s) 234
NlaIV GGNNCC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 41, 91, 216
PkrI GCNGC 2 cut(s) 105, 158
PspN4I GGNNCC 1 cut(s) 199
SatI GCNGC 2 cut(s) 104, 157
Sau3AI GATC 2 cut(s) 29, 223
SetI ASST 5 cut(s) 18, 51, 99, 193, 267
SfcI CTRYAG 1 cut(s) 36
SsiI CCGC 2 cut(s) 156, 260
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 3 cut(s) 115, 147, 163
TaiI ACGT 1 cut(s) 18
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 3 cut(s) 41, 91, 216
TseI GCWGC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.