RLG00000027046

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003666
Physical Location & Seq
Forward (+)
7912903 .. 7915015
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027046

Sequence Viewer

Length: 558 bp
ATGTGGAGTTTTCGCAATTTGCAGGCTGCAGATTCTCAGGTTGTTTCCAACTATGTGAGGCACTTTCTTCATCAGCACACCATTCAGCTATGGCAGCCTGCTACTGTCAAAGTTGTTGCTAACCTCATTAGGCTACTGTCATATGGAAACAAGGTTAGTTACTTGTATGGATTCTTTGATCAAGCATGGAGAGAAGGAATGACCAAGGACGAAGCGGAGAAGTTGGTTGTGAAGGCAGTTTCCCTAGCCATTGCACGAGATGGAGCCAGTGGTGGTGTTGTCCGCACTGTTGTGTTGCTTACATTTCTGACAGTACATGGTGACATGCATATTCTAGACATTGCTGTGGATTTGCCTTCTGTGACTGAAATTTTCAGCGGTGGGCAATTGAGCGGCTCAACTGATAGTGACACTAATGACGCAAAGAAAATTGGTGCTGAAGAAGATTGTGAGAATTTTCTTGGTTGCTTCAAAGATACGAATATGCAAGCCCAAGGTGTGGATTCCAAGAGGGGTTGGGGATTTCGTGGTGTGCACAAGGTATTTTTACTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

186

Amino Acids

20.5

Weight (kDa)

6.28

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 47 - 98 6.2e-08 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 393
AciI CCGC 4 cut(s) 215, 283, 378, 393
AcsI RAATTY 2 cut(s) 369, 454
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 1 cut(s) 472
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 1 cut(s) 88
AluI AGCT 1 cut(s) 88
Alw21I GWGCWC 1 cut(s) 537
Alw44I GTGCAC 1 cut(s) 533
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 2 cut(s) 26, 94
ApoI RAATTY 2 cut(s) 369, 454
AsuHPI GGTGA 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 537
BauI CACGAG 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 2 cut(s) 13, 106
BccI CCATC 1 cut(s) 254
BclI TGATCA 1 cut(s) 178
BfaI CTAG 2 cut(s) 245, 335
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 3 cut(s) 27, 95, 394
BlsI GCNGC 3 cut(s) 28, 96, 395
BmiI GGNNCC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 204, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 267
Bse3DI GCAATG 2 cut(s) 249, 339
BseDI CCNNGG 2 cut(s) 204, 493
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMI GCAATG 2 cut(s) 249, 339
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 2 cut(s) 13, 106
BsiHKAI GWGCWC 1 cut(s) 537
BslI CCNNNNNNNGG 1 cut(s) 499
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 4 cut(s) 215, 283, 378, 393
BspCNI CTCAG 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 265
BspMAI CTGCAG 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 249, 339
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 204, 493
BssMI GATC 1 cut(s) 178
BssSI CACGAG 1 cut(s) 255
BssT1I CCWWGG 2 cut(s) 204, 493
Bst2BI CACGAG 1 cut(s) 255
Bst4CI ACNGT 4 cut(s) 106, 138, 289, 313
BstC8I GCNNGC 3 cut(s) 24, 99, 489
BstDEI CTNAG 1 cut(s) 36
BstKTI GATC 1 cut(s) 181
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNSI RCATGY 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 13, 106
BtsIMutI CAGTG 2 cut(s) 274, 285
Cac8I GCNNGC 3 cut(s) 24, 99, 489
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 314
CviAII CATG 3 cut(s) 186, 317, 325
CviJI RGCY 8 cut(s) 26, 88, 97, 133, 248, 266, 396, 491
CviKI_1 RGCY 8 cut(s) 26, 88, 97, 133, 248, 266, 396, 491
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 1 cut(s) 36
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 204, 493
Eco57I CTGAAG 1 cut(s) 459
EcoT14I CCWWGG 2 cut(s) 204, 493
EcoT22I ATGCAT 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 204, 493
FaeI CATG 3 cut(s) 189, 320, 328
FatI CATG 3 cut(s) 185, 316, 324
FauNDI CATATG 1 cut(s) 142
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 3 cut(s) 27, 95, 394
Fsp4HI GCNGC 3 cut(s) 27, 95, 394
FspBI CTAG 2 cut(s) 245, 335
GluI GCNGC 3 cut(s) 27, 95, 394
HgaI GACGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 189, 320, 328
HinfI GANTC 3 cut(s) 32, 171, 503
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 3 cut(s) 188, 226, 366
HpyCH4III ACNGT 4 cut(s) 106, 138, 289, 313
HpyCH4V TGCA 6 cut(s) 22, 29, 254, 328, 487, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 1 cut(s) 36
Hsp92II CATG 3 cut(s) 189, 320, 328
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 4 cut(s) 8, 23, 111, 280
Lsp1109I GCAGC 2 cut(s) 13, 106
MaeI CTAG 2 cut(s) 245, 335
MaeIII GTNAC 4 cut(s) 158, 320, 361, 407
MalI GATC 1 cut(s) 180
MbiI CCGCTC 1 cut(s) 393
MboI GATC 1 cut(s) 178
MboII GAAGA 3 cut(s) 59, 452, 455
MfeI CAATTG 2 cut(s) 386, 553
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 6 cut(s) 16, 369, 386, 429, 454, 553
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 3 cut(s) 51, 134, 504
Mph1103I ATGCAT 1 cut(s) 330
MslI CAYNNNNRTG 2 cut(s) 290, 344
MspA1I CMGCKG 1 cut(s) 378
MunI CAATTG 2 cut(s) 386, 553
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 142
NdeII GATC 1 cut(s) 178
NlaIII CATG 3 cut(s) 189, 320, 328
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 3 cut(s) 320, 361, 407
NsiI ATGCAT 1 cut(s) 330
NspI RCATGY 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 290
PfeI GAWTC 3 cut(s) 32, 171, 503
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 3 cut(s) 28, 96, 395
PspN4I GGNNCC 1 cut(s) 265
PstI CTGCAG 1 cut(s) 31
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
RseI CAYNNNNRTG 2 cut(s) 290, 344
SatI GCNGC 3 cut(s) 27, 95, 394
Sau3AI GATC 1 cut(s) 178
SduI GDGCHC 1 cut(s) 537
SetI ASST 6 cut(s) 42, 90, 126, 156, 499, 543
SfcI CTRYAG 1 cut(s) 27
SmiMI CAYNNNNRTG 2 cut(s) 290, 344
Sse9I AATT 6 cut(s) 16, 369, 386, 429, 454, 553
SsiI CCGC 4 cut(s) 215, 283, 378, 393
SspMI CTAG 2 cut(s) 245, 335
StyI CCWWGG 2 cut(s) 204, 493
TaaI ACNGT 4 cut(s) 106, 138, 289, 313
TasI AATT 6 cut(s) 16, 369, 386, 429, 454, 553
TatI WGTACW 1 cut(s) 313
TauI GCSGC 1 cut(s) 396
TfiI GAWTC 3 cut(s) 32, 171, 503
TscAI CASTG 2 cut(s) 274, 292
TseFI GTSAC 3 cut(s) 320, 361, 407
TseI GCWGC 2 cut(s) 26, 94
Tsp45I GTSAC 3 cut(s) 320, 361, 407
TspDTI ATGAA 1 cut(s) 59
TspRI CASTG 2 cut(s) 274, 292
Van91I CCANNNNNTGG 1 cut(s) 499
VneI GTGCAC 1 cut(s) 533
XapI RAATTY 2 cut(s) 369, 454
XbaI TCTAGA 1 cut(s) 334
XceI RCATGY 1 cut(s) 328
XspI CTAG 2 cut(s) 245, 335
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.