Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

15952257 .. 15954803

Gene Structure 2547 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G33070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGACACCAAAATCGGATCGATCGATGATTGCAAGCCGACGAACGGCGACGTCTGTAGTCCAACAAACGGCACCGTCGCAACAATCCACAACTCTGTTCCTTCCTCCGCTATCACCATCAACTACTGCGACGCGACTCTCGGCCGTCACTTAGCTCGTCGTCTCGTCCAAGCCGGCGTTACGGATGTTTTCTCTGTTCCCGGAGATTTCAACCTCACTTTGCTTGATCACCTCATGGCTGAGCCGGACCTCAACCTAATCGGATGTTGTAACGAGCTAAACGCCGGTTACGCTGCCGACGGTTACGCTAGATCTCGTGGAGTCGGCGCTTGCGTTGTTACCTTCACCGTTGGTGGACTCAGCGTTTTAAACGCGATCGCTGGTGCTTACAGCGAGAATCTTCCTCTTATCTGTATCGTCGGAGGTCCTAACTCTAACGATTATGGCACTAACCGGATTCTTCATCACACCATTGGATTACCTGATTTTAGCCAAGAGCTTAGGTGCTTCCAAACGGTGACTTGTTATCAGGCGGTGGTGAACAATTTAGATGATGCTCATGAACAGATTGATAAAGCAATATCAACAGCTTTGAAAGAGAGCAAGCCTGTGTATATAAGTGTAAGCTGTAACTTAGCAGCGATTCCTCATCATACATTTAGCCGTGATCCTGTCCCTTTTTCTCTAGCTCCAAGATTGAGCAACAAGATGGGTTTAGAAGCTGCGGTGGAAGCAACATTGGAGTTTCTGAATAAGGCTGTGAAGCCAGTTATGGTTGGTGGTCCTAAGTTGCGTGTGGCTAAAGCTTGTGATGCCTTTGTTGAGCTAGCTGATGCTTCAGGCTATGCTTTGGCGATGATGCCTTCTGCGAAAGGCTTTGTACCAGAGCACCATCCTCATTTCATTGGAACTTATTGGGGAGCAGTGAGCACTCCTTTTTGCTCTGAGATTGTGGAATCTGCGGATGCTTACATTTTTGCAGGTCCAATCTTCAACGACTATAGCTCTGTTGGTTACTCGCTTCTCCTCAAGAAAGAAAAAGCCATCGTTGTGCAACCTGATCGTATCACTGTGGCCAATGGTCCTACTTTTGGTTGCATTTTGATGAGCGATTTCTTCAGGGAATTGTCTAAGAGGGTGAAGCGTAACGAGACTGCATATGAGAACTACCATAGGATCTTTGTCCCTGAAGGTAAGCCATTGAAGTGTGAATCAAGAGAGCCATTGAGAGTTAACACAATGTTCCAGCACATTCAGAAGATGCTCTCTAGTGAAACCGCTGTGATTGCTGAAACCGGTGATTCTTGGTTCAATTGCCAAAAACTAAAGCTGCCAAAAGGATGTGGGTACGAGTTTCAGATGCAGTATGGATCGATTGGGTGGTCTGTTGGTGCAACTCTAGGATACGCACAGGCATCACCAGAGAAGCGAGTGTTGGCATTCATCGGTGATGGGAGTTTCCAAGTCACGGTTCAGGACATATCAACAATGCTGCGTAATGGACAGAAGACGATCATCTTCTTGATTAACAATGGTGGCTACACCATTGAAGTAGAGATTCATGACGGTCCTTATAACGTGATTAAGAACTGGAACTACACTGGTCTCGTTGACGCCATTCATAACGGTGAAGGCAATTGCTGGACTGCAAAGGTGAGATACGAAGAGGAGTTAGTGGAGGCGATTACGACAGCGACGACGGAGAAGAAAGATTGTCTATGTTTCATAGAAGTGATTCTTCACAAGGATGATACGAGCAAAGAGTTGCTTGAGTGGGGCTCACGCGTCTCTGCTGCTAACAGCCGTCCTCCCAATCCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

607

Amino Acids

66.21

Weight (kDa)

5.7

Isoelectric Point (pI)

32.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	46 - 151	1e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	243 - 358	2.6e-21	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	453 - 577	4.5e-15	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1575
AatII	GACGTC	1 cut(s)	54
Acc36I	ACCTGC	1 cut(s)	971
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	3 cut(s)	134, 374, 1785
AciI	CCGC	5 cut(s)	108, 533, 725, 962, 1278
AclWI	GGATC	4 cut(s)	25, 662, 1184, 1378
AcoI	YGGCCR	2 cut(s)	142, 1074
AcuI	CTGAAG	3 cut(s)	822, 1102, 1209
AcyI	GRCGYC	2 cut(s)	51, 1614
AfaI	GTAC	2 cut(s)	882, 1349
AfiI	CCNNNNNNNGG	4 cut(s)	44, 68, 1091, 1468
AflIII	ACRYGT	1 cut(s)	1783
AgeI	ACCGGT	1 cut(s)	1295
AgsI	TTSAA	6 cut(s)	211, 595, 994, 1204, 1312, 1550
AjuI	GAANNNNNNNTTGG	4 cut(s)	1418, 1450, 1455, 1487
Alw21I	GWGCWC	2 cut(s)	891, 932
Alw26I	GTCTC	4 cut(s)	167, 1145, 1610, 1792
AlwI	GGATC	4 cut(s)	25, 662, 1184, 1378
AoxI	GGCC	2 cut(s)	142, 1074
ApeKI	GCWGC	6 cut(s)	293, 638, 722, 1330, 1492, 1793
AsiGI	ACCGGT	1 cut(s)	1295
AsiSI	GCGATCGC	1 cut(s)	378
Asp700I	GAANNNNTTC	1 cut(s)	1734
AspLEI	GCGC	1 cut(s)	329
AspS9I	GGNCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
AsuC2I	CCSGG	1 cut(s)	201
AsuNHI	GCTAGC	1 cut(s)	826
AvaII	GGWCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
BalI	TGGCCA	1 cut(s)	1076
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	1 cut(s)	1781
BauI	CACGAG	1 cut(s)	315
BbsI	GAAGAC	1 cut(s)	1514
Bbv12I	GWGCWC	2 cut(s)	891, 932
BbvI	GCAGC	6 cut(s)	280, 650, 709, 1317, 1479, 1780
BccI	CCATC	5 cut(s)	125, 703, 900, 1052, 1445
BceAI	ACGGC	5 cut(s)	61, 85, 129, 648, 1788
BcgI	CGANNNNNNTGC	2 cut(s)	1043, 1077
BciVI	GTATCC	1 cut(s)	1397
BclI	TGATCA	1 cut(s)	226
BcnI	CCSGG	1 cut(s)	201
BcoDI	GTCTC	4 cut(s)	167, 1145, 1610, 1792
BfaI	CTAG	5 cut(s)	309, 686, 827, 1269, 1400
BfmI	CTRYAG	2 cut(s)	55, 1000
BfoI	RGCGCY	1 cut(s)	330
BfuAI	ACCTGC	1 cut(s)	971
BfuI	GTATCC	1 cut(s)	1397
BglII	AGATCT	1 cut(s)	311
BisI	GCNGC	6 cut(s)	294, 639, 723, 1331, 1493, 1794
BlpI	GCTNAGC	1 cut(s)	240
BlsI	GCNGC	6 cut(s)	295, 640, 724, 1332, 1494, 1795
Bme1390I	CCNGG	1 cut(s)	201
Bme18I	GGWCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
BmgT120I	GGNCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
BmiI	GGNNCC	1 cut(s)	73
BmrFI	CCNGG	1 cut(s)	201
BmsI	GCATC	8 cut(s)	544, 802, 823, 849, 955, 1251, 1350, 1424
BmtI	GCTAGC	1 cut(s)	830
BpiI	GAAGAC	1 cut(s)	1514
BplI	GAGNNNNNCTC	2 cut(s)	1763, 1795
Bpu10I	CCTNAGC	1 cut(s)	500
Bpu1102I	GCTNAGC	1 cut(s)	240
BpuEI	CTTGAG	2 cut(s)	1013, 1790
BpuMI	CCSGG	1 cut(s)	201
Bsa29I	ATCGAT	3 cut(s)	20, 24, 1373
BsaHI	GRCGYC	2 cut(s)	51, 1614
BsaI	GGTCTC	1 cut(s)	1610
BsaWI	WCCGGW	2 cut(s)	453, 1295
Bsc4I	CCNNNNNNNGG	4 cut(s)	44, 68, 1091, 1468
Bse118I	RCCGGY	3 cut(s)	173, 284, 1295
Bse1I	ACTGG	3 cut(s)	767, 1595, 1606
BseCI	ATCGAT	3 cut(s)	20, 24, 1373
BseGI	GGATG	6 cut(s)	190, 269, 892, 970, 1346, 1753
BseLI	CCNNNNNNNGG	4 cut(s)	44, 68, 1091, 1468
BseMII	CTCAG	3 cut(s)	231, 373, 936
BseNI	ACTGG	3 cut(s)	767, 1595, 1606
BseRI	GAGGAG	2 cut(s)	1016, 1682
BseX3I	CGGCCG	1 cut(s)	142
BseXI	GCAGC	6 cut(s)	280, 650, 709, 1317, 1479, 1780
Bsh1236I	CGCG	3 cut(s)	134, 374, 1785
Bsh1285I	CGRYCG	3 cut(s)	24, 145, 378
BshFI	GGCC	2 cut(s)	144, 1076
BshNI	GGYRCC	1 cut(s)	71
BshTI	ACCGGT	1 cut(s)	1295
BshVI	ATCGAT	3 cut(s)	20, 24, 1373
BsiEI	CGRYCG	3 cut(s)	24, 145, 378
BsiHKAI	GWGCWC	2 cut(s)	891, 932
BsiSI	CCGG	6 cut(s)	174, 201, 245, 285, 454, 1296
BslFI	GGGAC	2 cut(s)	659, 1169
BslI	CCNNNNNNNGG	4 cut(s)	44, 68, 1091, 1468
BsmAI	GTCTC	4 cut(s)	167, 1145, 1610, 1792
BsmBI	CGTCTC	2 cut(s)	167, 1792
BsmFI	GGGAC	2 cut(s)	659, 1169
BsmI	GAATGC	1 cut(s)	1439
BsnI	GGCC	2 cut(s)	144, 1076
Bso31I	GGTCTC	1 cut(s)	1610
Bsp1286I	GDGCHC	3 cut(s)	891, 932, 1781
Bsp1720I	GCTNAGC	1 cut(s)	240
BspACI	CCGC	5 cut(s)	108, 533, 725, 962, 1278
BspANI	GGCC	2 cut(s)	144, 1076
BspCNI	CTCAG	3 cut(s)	232, 372, 937
BspDI	ATCGAT	3 cut(s)	20, 24, 1373
BspFNI	CGCG	3 cut(s)	134, 374, 1785
BspHI	TCATGA	2 cut(s)	559, 1561
BspLI	GGNNCC	1 cut(s)	73
BspMI	ACCTGC	1 cut(s)	971
BspOI	GCTAGC	1 cut(s)	830
BspPI	GGATC	4 cut(s)	25, 662, 1184, 1378
BspT107I	GGYRCC	1 cut(s)	71
BspTNI	GGTCTC	1 cut(s)	1610
BsrFI	RCCGGY	3 cut(s)	173, 284, 1295
BsrI	ACTGG	3 cut(s)	767, 1595, 1606
BssAI	RCCGGY	3 cut(s)	173, 284, 1295
BssNI	GRCGYC	2 cut(s)	51, 1614
BssSI	CACGAG	1 cut(s)	315
Bst2BI	CACGAG	1 cut(s)	315
Bst4CI	ACNGT	8 cut(s)	76, 302, 349, 517, 1072, 1471, 1568, 1628
Bst6I	CTCTTC	1 cut(s)	1659
BstACI	GRCGYC	2 cut(s)	51, 1614
BstC8I	GCNNGC	5 cut(s)	35, 175, 331, 605, 828
BstDEI	CTNAG	9 cut(s)	151, 240, 359, 500, 634, 786, 945, 1131, 1818
BstF5I	GGATG	6 cut(s)	190, 269, 892, 970, 1346, 1753
BstFNI	CGCG	3 cut(s)	134, 374, 1785
BstH2I	RGCGCY	1 cut(s)	330
BstHHI	GCGC	1 cut(s)	329
BstMAI	GTCTC	4 cut(s)	167, 1145, 1610, 1792
BstMCI	CGRYCG	3 cut(s)	24, 145, 378
BstMWI	GCNNNNNNNGC	4 cut(s)	290, 731, 812, 1286
BstSCI	CCNGG	1 cut(s)	199
BstSFI	CTRYAG	2 cut(s)	55, 1000
BstUI	CGCG	3 cut(s)	134, 374, 1785
BstV1I	GCAGC	6 cut(s)	280, 650, 709, 1317, 1479, 1780
BstV2I	GAAGAC	1 cut(s)	1514
BstX2I	RGATCY	2 cut(s)	311, 1176
BstYI	RGATCY	2 cut(s)	311, 1176
BstZI	CGGCCG	1 cut(s)	142
Bsu15I	ATCGAT	3 cut(s)	20, 24, 1373
BsuI	GTATCC	1 cut(s)	1397
BsuRI	GGCC	2 cut(s)	144, 1076
BsuTUI	ATCGAT	3 cut(s)	20, 24, 1373
BtgZI	GCGATG	1 cut(s)	869
BtsCI	GGATG	6 cut(s)	190, 269, 892, 970, 1346, 1753
BtsI	GCAGTG	1 cut(s)	930
BtsIMutI	CAGTG	3 cut(s)	930, 1068, 1599
BveI	ACCTGC	1 cut(s)	971
Cac8I	GCNNGC	5 cut(s)	35, 175, 331, 605, 828
CciI	TCATGA	2 cut(s)	559, 1561
CfoI	GCGC	1 cut(s)	329
Cfr10I	RCCGGY	3 cut(s)	173, 284, 1295
Cfr13I	GGNCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
ClaI	ATCGAT	3 cut(s)	20, 24, 1373
CseI	GACGC	3 cut(s)	140, 1622, 1774
Csp6I	GTAC	2 cut(s)	881, 1348
CspAI	ACCGGT	1 cut(s)	1295
CviAII	CATG	3 cut(s)	235, 560, 1562
CviQI	GTAC	2 cut(s)	881, 1348
DdeI	CTNAG	9 cut(s)	151, 240, 359, 500, 634, 786, 945, 1131, 1818
DraI	TTTAAA	1 cut(s)	369
EaeI	YGGCCR	2 cut(s)	142, 1074
EagI	CGGCCG	1 cut(s)	142
Eam1104I	CTCTTC	1 cut(s)	1659
EarI	CTCTTC	1 cut(s)	1659
EclXI	CGGCCG	1 cut(s)	142
Eco24I	GRGCYC	1 cut(s)	1781
Eco31I	GGTCTC	1 cut(s)	1610
Eco47I	GGWCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
Eco52I	CGGCCG	1 cut(s)	142
Eco57I	CTGAAG	3 cut(s)	822, 1102, 1209
EcoO109I	RGGNCCY	1 cut(s)	425
EcoT38I	GRGCYC	1 cut(s)	1781
Esp3I	CGTCTC	2 cut(s)	167, 1792
FaeI	CATG	3 cut(s)	238, 563, 1565
FalI	AAGNNNNNCTT	4 cut(s)	1722, 1754, 1752, 1784
FaqI	GGGAC	2 cut(s)	659, 1169
FatI	CATG	3 cut(s)	234, 559, 1561
FauNDI	CATATG	1 cut(s)	1159
FbaI	TGATCA	1 cut(s)	226
Fnu4HI	GCNGC	6 cut(s)	294, 639, 723, 1331, 1493, 1794
FokI	GGATG	6 cut(s)	197, 276, 879, 977, 1353, 1760
FriOI	GRGCYC	1 cut(s)	1781
Fsp4HI	GCNGC	6 cut(s)	294, 639, 723, 1331, 1493, 1794
FspBI	CTAG	5 cut(s)	309, 686, 827, 1269, 1400
GlaI	GCGC	1 cut(s)	328
GluI	GCNGC	6 cut(s)	294, 639, 723, 1331, 1493, 1794
HaeII	RGCGCY	1 cut(s)	330
HaeIII	GGCC	2 cut(s)	144, 1076
HapII	CCGG	6 cut(s)	174, 201, 245, 285, 454, 1296
HgaI	GACGC	3 cut(s)	140, 1622, 1774
HhaI	GCGC	1 cut(s)	329
Hin1I	GRCGYC	2 cut(s)	51, 1614
Hin1II	CATG	3 cut(s)	238, 563, 1565
Hin6I	GCGC	1 cut(s)	327
HinP1I	GCGC	1 cut(s)	327
HincII	GTYRAC	2 cut(s)	1234, 1612
HindII	GTYRAC	2 cut(s)	1234, 1612
HindIII	AAGCTT	1 cut(s)	804
HpaI	GTTAAC	1 cut(s)	1234
HpaII	CCGG	6 cut(s)	174, 201, 245, 285, 454, 1296
Hpy166II	GTNNAC	4 cut(s)	356, 541, 1234, 1612
Hpy188I	TCNGA	7 cut(s)	17, 263, 422, 750, 946, 1257, 1359
Hpy188III	TCNNGA	6 cut(s)	560, 1030, 1215, 1475, 1522, 1562
Hpy8I	GTNNAC	4 cut(s)	356, 541, 1234, 1612
Hpy99I	CGWCG	9 cut(s)	43, 53, 80, 134, 162, 302, 422, 1699, 1702
HpyAV	CCTTC	5 cut(s)	111, 352, 873, 1184, 1625
HpyCH4III	ACNGT	8 cut(s)	76, 302, 349, 517, 1072, 1471, 1568, 1628
HpyCH4IV	ACGT	2 cut(s)	51, 1578
HpyCH4V	TGCA	8 cut(s)	33, 980, 1054, 1098, 1157, 1363, 1394, 1649
HpyF10VI	GCNNNNNNNGC	4 cut(s)	290, 731, 812, 1286
HpyF3I	CTNAG	9 cut(s)	151, 240, 359, 500, 634, 786, 945, 1131, 1818
HpySE526I	ACGT	2 cut(s)	51, 1578
Hsp92I	GRCGYC	2 cut(s)	51, 1614
Hsp92II	CATG	3 cut(s)	238, 563, 1565
HspAI	GCGC	1 cut(s)	327
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	226
KspAI	GTTAAC	1 cut(s)	1234
LmnI	GCTCC	2 cut(s)	694, 920
Lsp1109I	GCAGC	6 cut(s)	280, 650, 709, 1317, 1479, 1780
LweI	GCATC	8 cut(s)	544, 802, 823, 849, 955, 1251, 1350, 1424
MaeI	CTAG	5 cut(s)	309, 686, 827, 1269, 1400
MaeII	ACGT	2 cut(s)	51, 1578
MfeI	CAATTG	2 cut(s)	1312, 1636
MflI	RGATCY	2 cut(s)	311, 1176
MhlI	GDGCHC	3 cut(s)	891, 932, 1781
MlsI	TGGCCA	1 cut(s)	1076
MluCI	AATT	4 cut(s)	544, 1124, 1312, 1636
MluI	ACGCGT	1 cut(s)	1783
MluNI	TGGCCA	1 cut(s)	1076
MlyI	GAGTC	3 cut(s)	130, 330, 351
MmeI	TCCRAC	2 cut(s)	86, 400
Mox20I	TGGCCA	1 cut(s)	1076
MroNI	GCCGGC	1 cut(s)	173
MroXI	GAANNNNTTC	1 cut(s)	1734
MscI	TGGCCA	1 cut(s)	1076
MseI	TTAA	4 cut(s)	368, 1233, 1527, 1584
MslI	CAYNNNNRTG	6 cut(s)	1049, 1103, 1204, 1626, 1730, 1746
Msp20I	TGGCCA	1 cut(s)	1076
MspA1I	CMGCKG	1 cut(s)	1280
MspI	CCGG	6 cut(s)	174, 201, 245, 285, 454, 1296
MspR9I	CCNGG	1 cut(s)	201
MunI	CAATTG	2 cut(s)	1312, 1636
Mva1269I	GAATGC	1 cut(s)	1439
MvnI	CGCG	3 cut(s)	134, 374, 1785
MwoI	GCNNNNNNNGC	4 cut(s)	290, 731, 812, 1286
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	1 cut(s)	201
NdeI	CATATG	1 cut(s)	1159
NgoMIV	GCCGGC	1 cut(s)	173
NheI	GCTAGC	1 cut(s)	826
NlaIII	CATG	3 cut(s)	238, 563, 1565
NlaIV	GGNNCC	1 cut(s)	73
NmeAIII	GCCGAG	1 cut(s)	120
NmuCI	GTSAC	3 cut(s)	146, 517, 1465
PagI	TCATGA	2 cut(s)	559, 1561
PcsI	WCGNNNNNNNCGW	3 cut(s)	21, 330, 1694
PctI	GAATGC	1 cut(s)	1439
PdiI	GCCGGC	1 cut(s)	175
PdmI	GAANNNNTTC	1 cut(s)	1734
PfeI	GAWTC	8 cut(s)	397, 457, 643, 956, 1211, 1301, 1558, 1735
PfoI	TCCNGGA	1 cut(s)	199
PinAI	ACCGGT	1 cut(s)	1295
PkrI	GCNGC	6 cut(s)	295, 640, 724, 1332, 1494, 1795
Ple19I	CGATCG	2 cut(s)	24, 378
PleI	GAGTC	3 cut(s)	130, 329, 351
PpsI	GAGTC	3 cut(s)	130, 329, 351
PpuMI	RGGWCCY	1 cut(s)	425
PsiI	TTATAA	1 cut(s)	1575
Psp5II	RGGWCCY	1 cut(s)	425
PspN4I	GGNNCC	1 cut(s)	73
PspPI	GGNCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
PspPPI	RGGWCCY	1 cut(s)	425
PsrI	GAACNNNNNNTAC	2 cut(s)	1580, 1612
PsuI	RGATCY	2 cut(s)	311, 1176
PvuI	CGATCG	2 cut(s)	24, 378
RgaI	GCGATCGC	1 cut(s)	378
RsaI	GTAC	2 cut(s)	882, 1349
RsaNI	GTAC	2 cut(s)	881, 1348
RseI	CAYNNNNRTG	6 cut(s)	1049, 1103, 1204, 1626, 1730, 1746
SaqAI	TTAA	4 cut(s)	368, 1233, 1527, 1584
SatI	GCNGC	6 cut(s)	294, 639, 723, 1331, 1493, 1794
Sau96I	GGNCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
SchI	GAGTC	3 cut(s)	130, 330, 351
ScrFI	CCNGG	1 cut(s)	201
SduI	GDGCHC	3 cut(s)	891, 932, 1781
SfaAI	GCGATCGC	1 cut(s)	378
SfaNI	GCATC	8 cut(s)	544, 802, 823, 849, 955, 1251, 1350, 1424
SfcI	CTRYAG	2 cut(s)	55, 1000
SgfI	GCGATCGC	1 cut(s)	378
SinI	GGWCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
SmiMI	CAYNNNNRTG	6 cut(s)	1049, 1103, 1204, 1626, 1730, 1746
SmlI	CTYRAG	2 cut(s)	1028, 1769
SmoI	CTYRAG	2 cut(s)	1028, 1769
Sse9I	AATT	4 cut(s)	544, 1124, 1312, 1636
SsiI	CCGC	5 cut(s)	108, 533, 725, 962, 1278
SspMI	CTAG	5 cut(s)	309, 686, 827, 1269, 1400
StyD4I	CCNGG	1 cut(s)	199
TaaI	ACNGT	8 cut(s)	76, 302, 349, 517, 1072, 1471, 1568, 1628
TaiI	ACGT	2 cut(s)	54, 1581
TaqI	TCGA	3 cut(s)	20, 24, 1373
TasI	AATT	4 cut(s)	544, 1124, 1312, 1636
TfiI	GAWTC	8 cut(s)	397, 457, 643, 956, 1211, 1301, 1558, 1735
Tru1I	TTAA	4 cut(s)	368, 1233, 1527, 1584
Tru9I	TTAA	4 cut(s)	368, 1233, 1527, 1584
TscAI	CASTG	3 cut(s)	930, 1075, 1606
TseFI	GTSAC	3 cut(s)	146, 517, 1465
TseI	GCWGC	6 cut(s)	293, 638, 722, 1330, 1492, 1793
Tsp45I	GTSAC	3 cut(s)	146, 517, 1465
TspDTI	ATGAA	7 cut(s)	452, 576, 892, 1432, 1550, 1610, 1714
TspGWI	ACGGA	2 cut(s)	197, 1715
TspRI	CASTG	3 cut(s)	930, 1075, 1606
VpaK11BI	GGWCC	6 cut(s)	247, 425, 782, 983, 1082, 1568
XmnI	GAANNNNTTC	1 cut(s)	1734
XspI	CTAG	5 cut(s)	309, 686, 827, 1269, 1400
ZraI	GACGTC	1 cut(s)	52

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.