Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

25279484 .. 25281198

Gene Structure 1715 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1172400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGACACAAAGATCGGATCCCTCGATGTCTGCAAGCCGGCGTGCACCGACGTCGGGAGCCTCCCGAACGGCGCCGCTTCGGCAATCCAGAGCTCTGCCCCCTCTACCGTGATCAACTCCTGCGACGCCACTCTTGGTCGCCACCTCGCGCGCCGACTCGTCCAAATCGGCGTCACCGACGTGTTCACTGTCCCTGGTGATTTTAACTTGACTCTGCTCGACCACCTCATCGCCGAGCCCGGACTCACCAACATCGGCTGCTGCAACGAACTCAACGCCGGGTACGCTGCTGACGGCTACGCGCGGTCGCGGGGAGTCGGCGCATGTGTTGTTACTTTCACTGTGGGTGGGCTCAGTGTTCTCAATGCAATTGCCGGAGCTTACAGTGAGAATCTTCCATTGATTTGTATAGTTGGAGGGCCGAACTCGAACGATTACGGGACGAACAGGATTCTTCACCACACTATTGGGTTGCCGGACTTTAGCCAAGAGCTGAGATGCTTTCAGACTGTAACTTGCTATCAGGCTGTGGTGAATAATCTGGAAGATGCGCATGAAATGATCGATACCGCAATTTCAACTGCTTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTCCTCATCCTACATTTAGCCGGGATCCTGTTCCATTTTCTTTATCTCCAAAATTGAGCAATAATTTGGGCTTAGAGGCTGCGGTGGGAGGCGGCTGCAGAGTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

249

Amino Acids

25.82

Weight (kDa)

5.24

Isoelectric Point (pI)

36.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	44 - 149	2e-30	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	54
Acc16I	TGCGCA	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	5 cut(s)	149, 151, 302, 304, 310
AciI	CCGC	6 cut(s)	75, 304, 310, 570, 719, 729
AclWI	GGATC	4 cut(s)	12, 25, 656, 669
AcsI	RAATTY	1 cut(s)	636
AcyI	GRCGYC	4 cut(s)	51, 72, 126, 171
AfaI	GTAC	1 cut(s)	284
AfiI	CCNNNNNNNGG	1 cut(s)	54
AflIII	ACRYGT	1 cut(s)	180
AgsI	TTSAA	2 cut(s)	579, 589
AjiI	CACGTC	1 cut(s)	181
AjnI	CCWGG	1 cut(s)	193
AleI	CACNNNNGTG	1 cut(s)	179
AluBI	AGCT	4 cut(s)	93, 380, 493, 621
AluI	AGCT	4 cut(s)	93, 380, 493, 621
Alw21I	GWGCWC	2 cut(s)	47, 95
Alw44I	GTGCAC	1 cut(s)	43
AlwI	GGATC	4 cut(s)	12, 25, 656, 669
AoxI	GGCC	1 cut(s)	419
ApaLI	GTGCAC	1 cut(s)	43
ApeKI	GCWGC	6 cut(s)	258, 261, 287, 621, 716, 732
ApoI	RAATTY	1 cut(s)	636
AspLEI	GCGC	6 cut(s)	74, 151, 153, 304, 323, 553
AspS9I	GGNCC	1 cut(s)	419
AsuC2I	CCSGG	3 cut(s)	240, 280, 659
AsuHPI	GGTGA	5 cut(s)	166, 209, 238, 449, 544
BaeGI	GKGCMC	1 cut(s)	47
BamHI	GGATCC	2 cut(s)	17, 661
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	3 cut(s)	95, 240, 354
Bbv12I	GWGCWC	2 cut(s)	47, 95
BbvI	GCAGC	6 cut(s)	245, 248, 274, 608, 703, 719
BceAI	ACGGC	2 cut(s)	85, 310
BcgI	CGANNNNNNTGC	2 cut(s)	34, 68
BciT130I	CCWGG	1 cut(s)	195
BclI	TGATCA	1 cut(s)	111
BcnI	CCSGG	3 cut(s)	240, 280, 659
BfmI	CTRYAG	1 cut(s)	733
BfoI	RGCGCY	1 cut(s)	75
BglI	GCCNNNNNGGC	1 cut(s)	80
BisI	GCNGC	8 cut(s)	75, 259, 262, 288, 622, 717, 730, 733
BlsI	GCNGC	8 cut(s)	76, 260, 263, 289, 623, 718, 731, 734
Bme1390I	CCNGG	4 cut(s)	195, 240, 280, 659
BmgBI	CACGTC	1 cut(s)	181
BmgT120I	GGNCC	1 cut(s)	419
BmiI	GGNNCC	4 cut(s)	19, 59, 73, 663
BmrFI	CCNGG	4 cut(s)	195, 240, 280, 659
BmsI	GCATC	2 cut(s)	488, 538
BpuMI	CCSGG	3 cut(s)	240, 280, 659
Bsa29I	ATCGAT	1 cut(s)	564
BsaHI	GRCGYC	4 cut(s)	51, 72, 126, 171
BsaJI	CCNNGG	1 cut(s)	193
Bsc4I	CCNNNNNNNGG	1 cut(s)	54
Bse118I	RCCGGY	1 cut(s)	37
BseBI	CCWGG	1 cut(s)	195
BseCI	ATCGAT	1 cut(s)	564
BseDI	CCNNGG	1 cut(s)	193
BseGI	GGATG	1 cut(s)	643
BseLI	CCNNNNNNNGG	1 cut(s)	54
BseMII	CTCAG	2 cut(s)	367, 485
BsePI	GCGCGC	1 cut(s)	149
BseSI	GKGCMC	1 cut(s)	47
BseXI	GCAGC	6 cut(s)	245, 248, 274, 608, 703, 719
Bsh1236I	CGCG	5 cut(s)	149, 151, 302, 304, 310
Bsh1285I	CGRYCG	1 cut(s)	308
BshFI	GGCC	1 cut(s)	421
BshNI	GGYRCC	1 cut(s)	71
BshVI	ATCGAT	1 cut(s)	564
BsiEI	CGRYCG	1 cut(s)	308
BsiHKAI	GWGCWC	2 cut(s)	47, 95
BsiSI	CCGG	6 cut(s)	38, 240, 279, 375, 476, 658
BslFI	GGGAC	2 cut(s)	176, 454
BslI	CCNNNNNNNGG	1 cut(s)	54
BsmFI	GGGAC	2 cut(s)	176, 454
BsnI	GGCC	1 cut(s)	421
Bsp1286I	GDGCHC	4 cut(s)	47, 95, 240, 354
Bsp143I	GATC	5 cut(s)	12, 17, 111, 561, 661
BspACI	CCGC	6 cut(s)	75, 304, 310, 570, 719, 729
BspANI	GGCC	1 cut(s)	421
BspCNI	CTCAG	2 cut(s)	366, 486
BspDI	ATCGAT	1 cut(s)	564
BspFNI	CGCG	5 cut(s)	149, 151, 302, 304, 310
BspLI	GGNNCC	4 cut(s)	19, 59, 73, 663
BspMAI	CTGCAG	1 cut(s)	737
BspPI	GGATC	4 cut(s)	12, 25, 656, 669
BspT107I	GGYRCC	1 cut(s)	71
BsrFI	RCCGGY	1 cut(s)	37
BssAI	RCCGGY	1 cut(s)	37
BssECI	CCNNGG	1 cut(s)	193
BssHII	GCGCGC	1 cut(s)	149
BssMI	GATC	5 cut(s)	12, 17, 111, 561, 661
BssNI	GRCGYC	4 cut(s)	51, 72, 126, 171
Bst2UI	CCWGG	1 cut(s)	195
Bst4CI	ACNGT	5 cut(s)	109, 190, 343, 386, 511
BstACI	GRCGYC	4 cut(s)	51, 72, 126, 171
BstAPI	GCANNNNNTGC	1 cut(s)	621
BstC8I	GCNNGC	5 cut(s)	35, 39, 43, 151, 599
BstDEI	CTNAG	3 cut(s)	353, 494, 709
BstF5I	GGATG	1 cut(s)	643
BstFNI	CGCG	5 cut(s)	149, 151, 302, 304, 310
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	6 cut(s)	74, 151, 153, 304, 323, 553
BstKTI	GATC	5 cut(s)	15, 20, 114, 564, 664
BstMBI	GATC	5 cut(s)	12, 17, 111, 561, 661
BstMCI	CGRYCG	1 cut(s)	308
BstMWI	GCNNNNNNNGC	3 cut(s)	80, 284, 621
BstNI	CCWGG	1 cut(s)	195
BstNSI	RCATGY	1 cut(s)	327
BstSCI	CCNGG	4 cut(s)	193, 238, 278, 657
BstSFI	CTRYAG	1 cut(s)	733
BstSLI	GKGCMC	1 cut(s)	47
BstUI	CGCG	5 cut(s)	149, 151, 302, 304, 310
BstV1I	GCAGC	6 cut(s)	245, 248, 274, 608, 703, 719
BstX2I	RGATCY	2 cut(s)	17, 661
BstXI	CCANNNNNNTGG	1 cut(s)	467
BstYI	RGATCY	2 cut(s)	17, 661
Bsu15I	ATCGAT	1 cut(s)	564
BsuRI	GGCC	1 cut(s)	421
BsuTUI	ATCGAT	1 cut(s)	564
BtgZI	GCGATG	1 cut(s)	214
BtrI	CACGTC	1 cut(s)	181
BtsCI	GGATG	1 cut(s)	643
BtsIMutI	CAGTG	4 cut(s)	186, 339, 361, 391
Cac8I	GCNNGC	5 cut(s)	35, 39, 43, 151, 599
CfoI	GCGC	6 cut(s)	74, 151, 153, 304, 323, 553
Cfr10I	RCCGGY	1 cut(s)	37
Cfr13I	GGNCC	1 cut(s)	419
ClaI	ATCGAT	1 cut(s)	564
CseI	GACGC	2 cut(s)	134, 160
Csp6I	GTAC	1 cut(s)	283
CviAII	CATG	2 cut(s)	324, 554
CviQI	GTAC	1 cut(s)	283
DdeI	CTNAG	3 cut(s)	353, 494, 709
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	5 cut(s)	14, 19, 113, 563, 663
DpnII	GATC	5 cut(s)	12, 17, 111, 561, 661
Ecl136II	GAGCTC	1 cut(s)	93
Eco24I	GRGCYC	3 cut(s)	95, 240, 354
Eco53kI	GAGCTC	1 cut(s)	93
EcoICRI	GAGCTC	1 cut(s)	93
EcoRI	GAATTC	1 cut(s)	636
EcoRII	CCWGG	1 cut(s)	193
EcoT38I	GRGCYC	3 cut(s)	95, 240, 354
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
FaeI	CATG	2 cut(s)	327, 557
FaiI	YATR	5 cut(s)	325, 410, 555, 609, 617
FaqI	GGGAC	2 cut(s)	176, 454
FatI	CATG	2 cut(s)	323, 553
FauI	CCCGC	1 cut(s)	303
FbaI	TGATCA	1 cut(s)	111
Fnu4HI	GCNGC	8 cut(s)	75, 259, 262, 288, 622, 717, 730, 733
FokI	GGATG	1 cut(s)	630
FriOI	GRGCYC	3 cut(s)	95, 240, 354
Fsp4HI	GCNGC	8 cut(s)	75, 259, 262, 288, 622, 717, 730, 733
FspAI	RTGCGCAY	1 cut(s)	552
FspI	TGCGCA	1 cut(s)	552
GlaI	GCGC	6 cut(s)	73, 150, 152, 303, 322, 552
GluI	GCNGC	8 cut(s)	75, 259, 262, 288, 622, 717, 730, 733
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	1 cut(s)	421
HapII	CCGG	6 cut(s)	38, 240, 279, 375, 476, 658
HgaI	GACGC	2 cut(s)	134, 160
HhaI	GCGC	6 cut(s)	74, 151, 153, 304, 323, 553
Hin1I	GRCGYC	4 cut(s)	51, 72, 126, 171
Hin1II	CATG	2 cut(s)	327, 557
Hin6I	GCGC	6 cut(s)	72, 149, 151, 302, 321, 551
HinP1I	GCGC	6 cut(s)	72, 149, 151, 302, 321, 551
HinfI	GANTC	6 cut(s)	156, 211, 243, 315, 391, 451
HpaII	CCGG	6 cut(s)	38, 240, 279, 375, 476, 658
HphI	GGTGA	5 cut(s)	166, 209, 238, 449, 544
Hpy166II	GTNNAC	2 cut(s)	45, 186
Hpy188I	TCNGA	2 cut(s)	17, 507
Hpy188III	TCNNGA	5 cut(s)	55, 64, 88, 542, 744
Hpy8I	GTNNAC	2 cut(s)	45, 186
Hpy99I	CGWCG	4 cut(s)	53, 56, 128, 182
HpyCH4III	ACNGT	5 cut(s)	109, 190, 343, 386, 511
HpyCH4IV	ACGT	2 cut(s)	51, 180
HpyCH4V	TGCA	6 cut(s)	33, 45, 264, 368, 624, 735
HpyF10VI	GCNNNNNNNGC	3 cut(s)	80, 284, 621
HpyF3I	CTNAG	3 cut(s)	353, 494, 709
HpySE526I	ACGT	2 cut(s)	51, 180
Hsp92I	GRCGYC	4 cut(s)	51, 72, 126, 171
Hsp92II	CATG	2 cut(s)	327, 557
HspAI	GCGC	6 cut(s)	72, 149, 151, 302, 321, 551
KasI	GGCGCC	1 cut(s)	71
KroI	GCCGGC	1 cut(s)	37
KroNI	GCCGGC	1 cut(s)	39
Ksp22I	TGATCA	1 cut(s)	111
Kzo9I	GATC	5 cut(s)	12, 17, 111, 561, 661
LmnI	GCTCC	2 cut(s)	57, 377
Lsp1109I	GCAGC	6 cut(s)	245, 248, 274, 608, 703, 719
LweI	GCATC	2 cut(s)	488, 538
MaeII	ACGT	2 cut(s)	51, 180
MaeIII	GTNAC	3 cut(s)	172, 331, 511
MalI	GATC	5 cut(s)	14, 19, 113, 563, 663
MboI	GATC	5 cut(s)	12, 17, 111, 561, 661
MboII	GAAGA	3 cut(s)	386, 446, 557
MfeI	CAATTG	1 cut(s)	369
MflI	RGATCY	2 cut(s)	17, 661
MhlI	GDGCHC	4 cut(s)	47, 95, 240, 354
MluCI	AATT	5 cut(s)	369, 573, 636, 689, 700
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	4 cut(s)	150, 205, 237, 324
MmeI	TCCRAC	1 cut(s)	394
MnlI	CCTC	9 cut(s)	32, 71, 112, 155, 236, 410, 651, 706, 719
MroNI	GCCGGC	1 cut(s)	37
MseI	TTAA	1 cut(s)	204
MslI	CAYNNNNRTG	1 cut(s)	179
MspI	CCGG	6 cut(s)	38, 240, 279, 375, 476, 658
MspR9I	CCNGG	4 cut(s)	195, 240, 280, 659
MunI	CAATTG	1 cut(s)	369
MvaI	CCWGG	1 cut(s)	195
MvnI	CGCG	5 cut(s)	149, 151, 302, 304, 310
MwoI	GCNNNNNNNGC	3 cut(s)	80, 284, 621
NaeI	GCCGGC	1 cut(s)	39
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	3 cut(s)	240, 280, 659
NdeII	GATC	5 cut(s)	12, 17, 111, 561, 661
NgoMIV	GCCGGC	1 cut(s)	37
NlaIII	CATG	2 cut(s)	327, 557
NlaIV	GGNNCC	4 cut(s)	19, 59, 73, 663
NmeAIII	GCCGAG	1 cut(s)	259
NmuCI	GTSAC	1 cut(s)	172
NsbI	TGCGCA	1 cut(s)	552
NspI	RCATGY	1 cut(s)	327
OliI	CACNNNNGTG	1 cut(s)	179
PauI	GCGCGC	1 cut(s)	149
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 174
PdiI	GCCGGC	1 cut(s)	39
PfeI	GAWTC	2 cut(s)	391, 451
PkrI	GCNGC	8 cut(s)	76, 260, 263, 289, 623, 718, 731, 734
PleI	GAGTC	4 cut(s)	150, 205, 237, 323
PluTI	GGCGCC	1 cut(s)	75
PpsI	GAGTC	4 cut(s)	150, 205, 237, 323
Psp124BI	GAGCTC	1 cut(s)	95
Psp6I	CCWGG	1 cut(s)	193
PspGI	CCWGG	1 cut(s)	193
PspN4I	GGNNCC	4 cut(s)	19, 59, 73, 663
PspPI	GGNCC	1 cut(s)	419
PstI	CTGCAG	1 cut(s)	737
PsuI	RGATCY	2 cut(s)	17, 661
PteI	GCGCGC	1 cut(s)	149
RsaI	GTAC	1 cut(s)	284
RsaNI	GTAC	1 cut(s)	283
RseI	CAYNNNNRTG	1 cut(s)	179
SacI	GAGCTC	1 cut(s)	95
SaqAI	TTAA	1 cut(s)	204
SatI	GCNGC	8 cut(s)	75, 259, 262, 288, 622, 717, 730, 733
Sau3AI	GATC	5 cut(s)	12, 17, 111, 561, 661
Sau96I	GGNCC	1 cut(s)	419
SchI	GAGTC	4 cut(s)	150, 205, 237, 324
ScrFI	CCNGG	4 cut(s)	195, 240, 280, 659
SduI	GDGCHC	4 cut(s)	47, 95, 240, 354
SetI	ASST	8 cut(s)	54, 95, 147, 183, 228, 382, 495, 623
SfaNI	GCATC	2 cut(s)	488, 538
SfcI	CTRYAG	1 cut(s)	733
SfoI	GGCGCC	1 cut(s)	73
SmiMI	CAYNNNNRTG	1 cut(s)	179
Sse9I	AATT	5 cut(s)	369, 573, 636, 689, 700
SsiI	CCGC	6 cut(s)	75, 304, 310, 570, 719, 729
SspDI	GGCGCC	1 cut(s)	71
SstI	GAGCTC	1 cut(s)	95
StyD4I	CCNGG	4 cut(s)	193, 238, 278, 657
TaaI	ACNGT	5 cut(s)	109, 190, 343, 386, 511
TaiI	ACGT	2 cut(s)	54, 183
TaqI	TCGA	4 cut(s)	24, 219, 428, 564
TasI	AATT	5 cut(s)	369, 573, 636, 689, 700
TauI	GCSGC	2 cut(s)	77, 732
TfiI	GAWTC	2 cut(s)	391, 451
Tru1I	TTAA	1 cut(s)	204
Tru9I	TTAA	1 cut(s)	204
TscAI	CASTG	4 cut(s)	193, 346, 361, 391
TseFI	GTSAC	1 cut(s)	172
TseI	GCWGC	6 cut(s)	258, 261, 287, 621, 716, 732
Tsp45I	GTSAC	1 cut(s)	172
TspDTI	ATGAA	1 cut(s)	570
TspRI	CASTG	4 cut(s)	193, 346, 361, 391
VneI	GTGCAC	1 cut(s)	43
XapI	RAATTY	1 cut(s)	636
XceI	RCATGY	1 cut(s)	327
ZraI	GACGTC	1 cut(s)	52

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.