Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

10241968 .. 10244670

Gene Structure 2703 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG120800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGACACCAAGATTGGCTCCCTCGACGTTTGCAAGGCCGACAACCACGACGTCGGTTGTTTACCAAACAGCGCCGCTTGCACCGTCCAAAACTCGGTCCCCTCCACCACGTTCAACTCCGCCGACGCCACCCTCGGCCGCCACCTGGCACGCCGCCTCGTCCAAATCGGCGTCACCGACGTCTTCACCGTCCCCGGCGACTTCAACTTGACCCTTCTCGACCACCTCATCGCCGAGCCCGGGCTCACCAACATTGGCTGCTGCAATGAGCTCAACGCCGGATACGCCGCCGACGGCTACGCGCGGTCGCGTGGCGTCGGGGCGTGTGCGGTGACTTTCACCGTCGGTGGACTGAGTGTGTTGAACGCGATCGCTGGCGCGTACAGTGAGAACTTGCCGGTGATTTGTATTGTTGGAGGGCCCAACTCCAATGATTACGGAACTAACAGGATTCTTCACCATACTATTGGGGTGCCGGACTTTAGCCAAGAGCTCCGGTGCTTTCAGACTGTGACTTGCTTTCAGGCTGTGGTGAATAATCTGGAGGATGCACATGAGTTGATTGATACGGCAATTTCGACTGCGTTGAAAGAAAGCAAGCCTGTGTATATCAGCATAGGCTGCAACTTGGCTGGGATTCCTCATCCTACTTTTAGCCGTGACCCTGTTCCATTTTCATTGTCTCCAAAATTGAGCAATAAGATGGGATTAGAGGCTGCAGTGGAGGCAGCGGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTGGGCGGGCCCAAATTGCGTGCTGCACATGCCGGTGATGCCTTTGTTGAGCTGGCTGATGCTTCTGGTTTTGCTCTGGCTGTTATGCCGTCAGCAAAGGGGCAAGTGCCAGAGCACCACCCCCATTTCATAGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGTGCTGAGATTGTGGAGTCCGCAGATGCATACTTGTTTGCTGGGCCGATTTTCAATGACTACAGCTCCGTTGGGTACTCGCTCCTTCTCAAGAAAGAGAAGGCGATCATTGTGCAGCCCGATCGTGTGACGATAGGAAATGGCCCTGCATTTGGTTGTGTTCTCATGAAGGATTTCCTGTTATGCCTAGCAAAGAAGCTGAAGCATAACAATACTGCTCATGAGAACTACCGCAGGATCTTTGTGCCCGATGGCCACCCTCTAAAGGCTGCACCCAGAGAACCTTTGAGGGTTAATGTTCTGTTCCAGCACATTCAGAAGATGCTGTCAGCTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTCAACTGCCAGAAGCTGAAATTGCCACCTGCCTGCGGGTATGAATTCCAAATGCAATATGGATCAATAGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAGGCTGTACCTGAGAAGCGAGTGCTTTCTTTCATTGGTGATGGGAGCTTCCAGGTGACTGCTCAAGATGTGTCCACAATGATTCGAAATGGACAGAGGACCATCATTTTCCTGATAAACAATGGCGGATACACCATTGAAGTGGAAATCCATGATGGACCATACAATGTGATCAAGAACTGGAACTACACTGGACTGGTTGATGCCATCCACAATGGGGAGGGCAAGTGCTGGACAACCAAGGTGCGTTGCGAAGAGGAGCTGATTGAAGCAATTGAGACTGCAAATGGACCCAAGAAGGACAGCTTGTGCTTCATTGAGGTGATTGTTCACAAGGATGATACCAGCAAAGAGTTGCTTGAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCTAATCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

605

Amino Acids

65.15

Weight (kDa)

5.85

Isoelectric Point (pI)

30.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	44 - 149	1e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	241 - 359	1.7e-22	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	451 - 576	1.8e-14	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1339
AatII	GACGTC	2 cut(s)	54, 183
Acc36I	ACCTGC	1 cut(s)	1339
AccB1I	GGYRCC	1 cut(s)	472
AccII	CGCG	5 cut(s)	302, 304, 310, 368, 380
AclWI	GGATC	2 cut(s)	1178, 1372
AcoI	YGGCCR	2 cut(s)	136, 1186
AcsI	RAATTY	1 cut(s)	1346
AcuI	CTGAAG	1 cut(s)	1154
AcyI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
AfaI	GTAC	3 cut(s)	383, 1010, 1413
AfiI	CCNNNNNNNGG	7 cut(s)	94, 145, 240, 1085, 1198, 1337, 1621
AgsI	TTSAA	9 cut(s)	115, 205, 364, 589, 745, 988, 1306, 1544, 1673
AjnI	CCWGG	2 cut(s)	144, 1455
AloI	GAACNNNNNNTCC	2 cut(s)	1286, 1318
Alw21I	GWGCWC	4 cut(s)	273, 495, 885, 926
Alw26I	GTCTC	3 cut(s)	687, 1280, 1676
AlwI	GGATC	2 cut(s)	1178, 1372
AlwNI	CAGNNNCTG	1 cut(s)	1318
Ama87I	CYCGRG	1 cut(s)	239
AoxI	GGCC	7 cut(s)	36, 136, 419, 776, 977, 1075, 1186
ApaI	GGGCCC	2 cut(s)	423, 780
ApeKI	GCWGC	9 cut(s)	258, 261, 621, 716, 728, 791, 1048, 1202, 1787
ApoI	RAATTY	1 cut(s)	1346
AsiSI	GCGATCGC	1 cut(s)	372
Asp700I	GAANNNNTTC	1 cut(s)	1106
AspLEI	GCGC	3 cut(s)	74, 304, 380
AsuC2I	CCSGG	3 cut(s)	195, 240, 241
AsuII	TTCGAA	1 cut(s)	1489
AvaI	CYCGRG	1 cut(s)	239
AvaII	GGWCC	4 cut(s)	97, 1503, 1562, 1694
BaeGI	GKGCMC	3 cut(s)	423, 780, 1182
BalI	TGGCCA	1 cut(s)	1188
BanI	GGYRCC	1 cut(s)	472
BanII	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
BbsI	GAAGAC	1 cut(s)	175
Bbv12I	GWGCWC	4 cut(s)	273, 495, 885, 926
BbvI	GCAGC	9 cut(s)	245, 248, 608, 703, 740, 778, 1060, 1189, 1774
BccI	CCATC	6 cut(s)	697, 1178, 1439, 1514, 1553, 1619
BceAI	ACGGC	4 cut(s)	310, 585, 642, 841
BcgI	CGANNNNNNTGC	4 cut(s)	1027, 1037, 1061, 1071
BciT130I	CCWGG	2 cut(s)	146, 1457
BciVI	GTATCC	2 cut(s)	275, 1526
BclI	TGATCA	1 cut(s)	1575
BcnI	CCSGG	3 cut(s)	195, 240, 241
BcoDI	GTCTC	3 cut(s)	687, 1280, 1676
BfaI	CTAG	2 cut(s)	1121, 1776
BfmI	CTRYAG	2 cut(s)	717, 994
BfoI	RGCGCY	1 cut(s)	75
BfuAI	ACCTGC	1 cut(s)	1339
BfuI	GTATCC	2 cut(s)	275, 1526
Bme1390I	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
Bme18I	GGWCC	4 cut(s)	97, 1503, 1562, 1694
BmeT110I	CYCGRG	1 cut(s)	239
BmiI	GGNNCC	6 cut(s)	19, 99, 421, 474, 778, 1696
BmrFI	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
BmrI	ACTGGG	1 cut(s)	919
BmsI	GCATC	6 cut(s)	538, 796, 817, 949, 1245, 1597
BmuI	ACTGGG	1 cut(s)	919
BpiI	GAAGAC	1 cut(s)	175
BpmI	CTGGAG	1 cut(s)	563
Bpu10I	CCTNAGC	1 cut(s)	1404
Bpu14I	TTCGAA	1 cut(s)	1489
BpuEI	CTTGAG	3 cut(s)	1007, 1452, 1784
BpuMI	CCSGG	3 cut(s)	195, 240, 241
BsaHI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
BsaJI	CCNNGG	4 cut(s)	133, 193, 239, 1644
BsaWI	WCCGGW	1 cut(s)	495
BsaXI	ACNNNNNCTCC	4 cut(s)	983, 1013, 1616, 1646
Bsc4I	CCNNNNNNNGG	7 cut(s)	94, 145, 240, 1085, 1198, 1337, 1621
Bse118I	RCCGGY	3 cut(s)	397, 760, 800
Bse1I	ACTGG	4 cut(s)	914, 1589, 1600, 1605
Bse3DI	GCAATG	1 cut(s)	271
BseBI	CCWGG	2 cut(s)	146, 1457
BseDI	CCNNGG	4 cut(s)	133, 193, 239, 1644
BseGI	GGATG	4 cut(s)	553, 643, 1611, 1747
BseLI	CCNNNNNNNGG	7 cut(s)	94, 145, 240, 1085, 1198, 1337, 1621
BseMI	GCAATG	1 cut(s)	271
BseMII	CTCAG	5 cut(s)	344, 930, 1275, 1407, 1418
BseNI	ACTGG	4 cut(s)	914, 1589, 1600, 1605
BseRI	GAGGAG	1 cut(s)	1676
BseSI	GKGCMC	3 cut(s)	423, 780, 1182
BseX3I	CGGCCG	1 cut(s)	136
BseXI	GCAGC	9 cut(s)	245, 248, 608, 703, 740, 778, 1060, 1189, 1774
BseYI	CCCAGC	2 cut(s)	632, 974
BsgI	GTGCAG	3 cut(s)	777, 1067, 1188
Bsh1236I	CGCG	5 cut(s)	302, 304, 310, 368, 380
Bsh1285I	CGRYCG	4 cut(s)	139, 308, 372, 1057
BshFI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BshNI	GGYRCC	1 cut(s)	472
BsiEI	CGRYCG	4 cut(s)	139, 308, 372, 1057
BsiHKAI	GWGCWC	4 cut(s)	273, 495, 885, 926
BsiHKCI	CYCGRG	1 cut(s)	239
BsiSI	CCGG	8 cut(s)	195, 240, 279, 398, 476, 496, 761, 801
BslFI	GGGAC	3 cut(s)	83, 176, 1307
BslI	CCNNNNNNNGG	7 cut(s)	94, 145, 240, 1085, 1198, 1337, 1621
BsmAI	GTCTC	3 cut(s)	687, 1280, 1676
BsmFI	GGGAC	3 cut(s)	83, 176, 1307
BsnI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BsoBI	CYCGRG	1 cut(s)	239
Bsp119I	TTCGAA	1 cut(s)	1489
Bsp120I	GGGCCC	2 cut(s)	419, 776
Bsp1286I	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
Bsp143I	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
BspANI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BspCNI	CTCAG	5 cut(s)	345, 931, 1276, 1408, 1417
BspFNI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BspHI	TCATGA	2 cut(s)	1098, 1153
BspLI	GGNNCC	6 cut(s)	19, 99, 421, 474, 778, 1696
BspMAI	CTGCAG	1 cut(s)	721
BspMI	ACCTGC	1 cut(s)	1339
BspPI	GGATC	2 cut(s)	1178, 1372
BspT104I	TTCGAA	1 cut(s)	1489
BspT107I	GGYRCC	1 cut(s)	472
BsrDI	GCAATG	1 cut(s)	271
BsrFI	RCCGGY	3 cut(s)	397, 760, 800
BsrI	ACTGG	4 cut(s)	914, 1589, 1600, 1605
BssAI	RCCGGY	3 cut(s)	397, 760, 800
BssECI	CCNNGG	4 cut(s)	133, 193, 239, 1644
BssMI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
BssNI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
BssT1I	CCWWGG	1 cut(s)	1644
Bst2UI	CCWGG	2 cut(s)	146, 1457
Bst4CI	ACNGT	5 cut(s)	85, 190, 343, 386, 511
Bst6I	CTCTTC	1 cut(s)	1653
BstACI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
BstAPI	GCANNNNNTGC	1 cut(s)	621
BstBI	TTCGAA	1 cut(s)	1489
BstC8I	GCNNGC	8 cut(s)	79, 151, 376, 599, 776, 789, 822, 1336
BstDEI	CTNAG	6 cut(s)	353, 939, 1284, 1404, 1416, 1812
BstF5I	GGATG	4 cut(s)	553, 643, 1611, 1747
BstFNI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	3 cut(s)	74, 304, 380
BstKTI	GATC	6 cut(s)	372, 1041, 1057, 1173, 1367, 1578
BstMAI	GTCTC	3 cut(s)	687, 1280, 1676
BstMBI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
BstMCI	CGRYCG	4 cut(s)	139, 308, 372, 1057
BstNI	CCWGG	2 cut(s)	146, 1457
BstNSI	RCATGY	1 cut(s)	800
BstSCI	CCNGG	5 cut(s)	144, 193, 238, 239, 1455
BstSFI	CTRYAG	2 cut(s)	717, 994
BstSLI	GKGCMC	3 cut(s)	423, 780, 1182
BstUI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BstV1I	GCAGC	9 cut(s)	245, 248, 608, 703, 740, 778, 1060, 1189, 1774
BstV2I	GAAGAC	1 cut(s)	175
BstX2I	RGATCY	1 cut(s)	1170
BstXI	CCANNNNNNTGG	2 cut(s)	467, 1546
BstYI	RGATCY	1 cut(s)	1170
BstZI	CGGCCG	1 cut(s)	136
BsuI	GTATCC	2 cut(s)	275, 1526
BsuRI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BtgZI	GCGATG	1 cut(s)	214
BtsCI	GGATG	4 cut(s)	553, 643, 1611, 1747
BtsI	GCAGTG	3 cut(s)	726, 759, 924
BtsIMutI	CAGTG	5 cut(s)	391, 726, 759, 924, 1593
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	8 cut(s)	79, 151, 376, 599, 776, 789, 822, 1336
CaiI	CAGNNNCTG	1 cut(s)	1318
CciI	TCATGA	2 cut(s)	1098, 1153
CfoI	GCGC	3 cut(s)	74, 304, 380
Cfr10I	RCCGGY	3 cut(s)	397, 760, 800
Cfr9I	CCCGGG	1 cut(s)	239
CseI	GACGC	3 cut(s)	134, 160, 304
Csp6I	GTAC	3 cut(s)	382, 1009, 1412
CviAII	CATG	6 cut(s)	554, 797, 1099, 1154, 1299, 1556
CviQI	GTAC	3 cut(s)	382, 1009, 1412
DdeI	CTNAG	6 cut(s)	353, 939, 1284, 1404, 1416, 1812
DpnI	GATC	6 cut(s)	371, 1040, 1056, 1172, 1366, 1577
DpnII	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
EaeI	YGGCCR	2 cut(s)	136, 1186
EagI	CGGCCG	1 cut(s)	136
Eam1104I	CTCTTC	1 cut(s)	1653
EarI	CTCTTC	1 cut(s)	1653
EciI	GGCGGA	2 cut(s)	109, 1545
Ecl136II	GAGCTC	2 cut(s)	271, 493
EclXI	CGGCCG	1 cut(s)	136
Eco130I	CCWWGG	1 cut(s)	1644
Eco24I	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
Eco47I	GGWCC	4 cut(s)	97, 1503, 1562, 1694
Eco52I	CGGCCG	1 cut(s)	136
Eco53kI	GAGCTC	2 cut(s)	271, 493
Eco57I	CTGAAG	1 cut(s)	1154
Eco88I	CYCGRG	1 cut(s)	239
EcoICRI	GAGCTC	2 cut(s)	271, 493
EcoO109I	RGGNCCY	1 cut(s)	419
EcoRI	GAATTC	1 cut(s)	1346
EcoRII	CCWGG	2 cut(s)	144, 1455
EcoT14I	CCWWGG	1 cut(s)	1644
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
ErhI	CCWWGG	1 cut(s)	1644
FaeI	CATG	6 cut(s)	557, 800, 1102, 1157, 1302, 1559
FalI	AAGNNNNNCTT	4 cut(s)	1694, 1726, 1746, 1778
FaqI	GGGAC	3 cut(s)	83, 176, 1307
FatI	CATG	6 cut(s)	553, 796, 1098, 1153, 1298, 1555
FauI	CCCGC	2 cut(s)	767, 1331
FbaI	TGATCA	1 cut(s)	1575
FokI	GGATG	4 cut(s)	560, 630, 1598, 1754
FriOI	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
FspBI	CTAG	2 cut(s)	1121, 1776
GlaI	GCGC	3 cut(s)	73, 303, 379
GsaI	CCCAGC	2 cut(s)	636, 978
GsuI	CTGGAG	1 cut(s)	563
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
HapII	CCGG	8 cut(s)	195, 240, 279, 398, 476, 496, 761, 801
HgaI	GACGC	3 cut(s)	134, 160, 304
HhaI	GCGC	3 cut(s)	74, 304, 380
Hin1I	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
Hin1II	CATG	6 cut(s)	557, 800, 1102, 1157, 1302, 1559
Hin6I	GCGC	3 cut(s)	72, 302, 378
HinP1I	GCGC	3 cut(s)	72, 302, 378
HinfI	GANTC	5 cut(s)	451, 637, 950, 1295, 1486
HpaII	CCGG	8 cut(s)	195, 240, 279, 398, 476, 496, 761, 801
Hpy166II	GTNNAC	4 cut(s)	62, 350, 1479, 1735
Hpy188I	TCNGA	2 cut(s)	507, 1251
Hpy188III	TCNNGA	9 cut(s)	218, 542, 742, 1024, 1099, 1154, 1469, 1516, 1579
Hpy8I	GTNNAC	4 cut(s)	62, 350, 1479, 1735
Hpy99I	CGWCG	8 cut(s)	29, 53, 56, 128, 182, 296, 320, 347
HpyAV	CCTTC	5 cut(s)	224, 1027, 1028, 1096, 1696
HpyCH4III	ACNGT	5 cut(s)	85, 190, 343, 386, 511
HpyCH4IV	ACGT	4 cut(s)	27, 51, 110, 180
HpyF3I	CTNAG	6 cut(s)	353, 939, 1284, 1404, 1416, 1812
HpySE526I	ACGT	4 cut(s)	27, 51, 110, 180
Hsp92I	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
Hsp92II	CATG	6 cut(s)	557, 800, 1102, 1157, 1302, 1559
HspAI	GCGC	3 cut(s)	72, 302, 378
Ksp22I	TGATCA	1 cut(s)	1575
Kzo9I	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
LmnI	GCTCC	7 cut(s)	23, 498, 1004, 1020, 1385, 1449, 1663
Lsp1109I	GCAGC	9 cut(s)	245, 248, 608, 703, 740, 778, 1060, 1189, 1774
LweI	GCATC	6 cut(s)	538, 796, 817, 949, 1245, 1597
MaeI	CTAG	2 cut(s)	1121, 1776
MaeII	ACGT	4 cut(s)	27, 51, 110, 180
MaeIII	GTNAC	6 cut(s)	172, 331, 511, 659, 1060, 1459
MalI	GATC	6 cut(s)	371, 1040, 1056, 1172, 1366, 1577
MboI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
MboII	GAAGA	4 cut(s)	175, 446, 1264, 1670
MfeI	CAATTG	1 cut(s)	1677
MflI	RGATCY	1 cut(s)	1170
MhlI	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
MlsI	TGGCCA	1 cut(s)	1188
MluCI	AATT	6 cut(s)	573, 689, 782, 1322, 1346, 1677
MluNI	TGGCCA	1 cut(s)	1188
MlyI	GAGTC	2 cut(s)	959, 1289
MmeI	TCCRAC	2 cut(s)	394, 1363
Mox20I	TGGCCA	1 cut(s)	1188
Mph1103I	ATGCAT	1 cut(s)	964
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	1 cut(s)	1188
MseI	TTAA	1 cut(s)	1227
MslI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
Msp20I	TGGCCA	1 cut(s)	1188
MspA1I	CMGCKG	2 cut(s)	731, 1265
MspI	CCGG	8 cut(s)	195, 240, 279, 398, 476, 496, 761, 801
MspR9I	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
MunI	CAATTG	1 cut(s)	1677
MvaI	CCWGG	2 cut(s)	146, 1457
MvnI	CGCG	5 cut(s)	302, 304, 310, 368, 380
NciI	CCSGG	3 cut(s)	195, 240, 241
NdeII	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
NlaIII	CATG	6 cut(s)	557, 800, 1102, 1157, 1302, 1559
NlaIV	GGNNCC	6 cut(s)	19, 99, 421, 474, 778, 1696
NmeAIII	GCCGAG	2 cut(s)	114, 259
NmuCI	GTSAC	6 cut(s)	172, 331, 511, 659, 1060, 1459
NsiI	ATGCAT	1 cut(s)	964
NspI	RCATGY	1 cut(s)	800
NspV	TTCGAA	1 cut(s)	1489
PagI	TCATGA	2 cut(s)	1098, 1153
PaqCI	CACCTGC	1 cut(s)	1339
PcsI	WCGNNNNNNNCGW	3 cut(s)	174, 186, 575
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	3 cut(s)	451, 637, 1486
Ple19I	CGATCG	2 cut(s)	372, 1057
PleI	GAGTC	2 cut(s)	958, 1289
PpsI	GAGTC	2 cut(s)	958, 1289
Psp124BI	GAGCTC	2 cut(s)	273, 495
Psp6I	CCWGG	2 cut(s)	144, 1455
PspFI	CCCAGC	2 cut(s)	632, 974
PspGI	CCWGG	2 cut(s)	144, 1455
PspN4I	GGNNCC	6 cut(s)	19, 99, 421, 474, 778, 1696
PspOMI	GGGCCC	2 cut(s)	419, 776
PsrI	GAACNNNNNNTAC	2 cut(s)	1574, 1606
PstI	CTGCAG	1 cut(s)	721
PstNI	CAGNNNCTG	1 cut(s)	1318
PsuI	RGATCY	1 cut(s)	1170
PvuI	CGATCG	2 cut(s)	372, 1057
PvuII	CAGCTG	1 cut(s)	1265
RgaI	GCGATCGC	1 cut(s)	372
RsaI	GTAC	3 cut(s)	383, 1010, 1413
RsaNI	GTAC	3 cut(s)	382, 1009, 1412
RseI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
SacI	GAGCTC	2 cut(s)	273, 495
SaqAI	TTAA	1 cut(s)	1227
Sau3AI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1575
SchI	GAGTC	2 cut(s)	959, 1289
ScrFI	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
SduI	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
SfaAI	GCGATCGC	1 cut(s)	372
SfaNI	GCATC	6 cut(s)	538, 796, 817, 949, 1245, 1597
SfcI	CTRYAG	2 cut(s)	717, 994
SfuI	TTCGAA	1 cut(s)	1489
SgfI	GCGATCGC	1 cut(s)	372
SinI	GGWCC	4 cut(s)	97, 1503, 1562, 1694
SmaI	CCCGGG	1 cut(s)	241
SmiMI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
SmlI	CTYRAG	3 cut(s)	1022, 1467, 1763
SmoI	CTYRAG	3 cut(s)	1022, 1467, 1763
SrfI	GCCCGGGC	1 cut(s)	241
Sse9I	AATT	6 cut(s)	573, 689, 782, 1322, 1346, 1677
SspMI	CTAG	2 cut(s)	1121, 1776
SstI	GAGCTC	2 cut(s)	273, 495
StyD4I	CCNGG	5 cut(s)	144, 193, 238, 239, 1455
StyI	CCWWGG	1 cut(s)	1644
TaaI	ACNGT	5 cut(s)	85, 190, 343, 386, 511
TaiI	ACGT	4 cut(s)	30, 54, 113, 183
TaqI	TCGA	4 cut(s)	24, 219, 578, 1489
TaqII	GACCGA	1 cut(s)	85
TasI	AATT	6 cut(s)	573, 689, 782, 1322, 1346, 1677
TauI	GCSGC	6 cut(s)	77, 141, 156, 290, 734, 1800
TfiI	GAWTC	3 cut(s)	451, 637, 1486
Tru1I	TTAA	1 cut(s)	1227
Tru9I	TTAA	1 cut(s)	1227
TscAI	CASTG	5 cut(s)	391, 726, 759, 931, 1600
TseFI	GTSAC	6 cut(s)	172, 331, 511, 659, 1060, 1459
TseI	GCWGC	9 cut(s)	258, 261, 621, 716, 728, 791, 1048, 1202, 1787
Tsp45I	GTSAC	6 cut(s)	172, 331, 511, 659, 1060, 1459
TspDTI	ATGAA	6 cut(s)	666, 886, 1115, 1359, 1426, 1708
TspGWI	ACGGA	2 cut(s)	453, 991
TspMI	CCCGGG	1 cut(s)	239
TspRI	CASTG	5 cut(s)	391, 726, 759, 931, 1600
VpaK11BI	GGWCC	4 cut(s)	97, 1503, 1562, 1694
XapI	RAATTY	1 cut(s)	1346
XceI	RCATGY	1 cut(s)	800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmaI	CCCGGG	1 cut(s)	239
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	2 cut(s)	1121, 1776
ZraI	GACGTC	2 cut(s)	52, 181
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.