Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

25021048 .. 25024086

Gene Structure 3039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1159900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGACACCAAAATTGGTTCGCTTGACGTCTGCAAGCCTACGTGCACCGGCGTCGGCAGCCTACCGAACGGCGCCGCTTTAGCAATCCAAAGCTCTGCCCCCTCCCTCATCAACTCCTCTGACGCCACTCTGGGTGGCCACATCGCCCGCCGACTTGTCCAAATCGGCGTCACGGACGTGTTCACTGTCCCAGGTGACTTTAACTTAACCCTCCTAGACCACCTCATTGCCGAGCCTGGGCTCACCAACATCGGCTGCTGCAACGAACTCAATGCCGGGTACGCTGCTGACGGCTACGCTCGGTCGCGGGGAGTCGGGGCGTGTGTTGTTACTTTCACTGTGGGTGGGCTCAGTGTTCTCAATGCTATCGCGGGAGCTTACAGTGAGAGTCTGCCATTGATTTGTATAGTTGGAGGACCCAACTCGAATGATTACGGGACGCACAGGATTCTTCACCACACTATTGGGTTACCGGATTTTAGCCAAGAGTTGACATGCTTCCAGACCGTCACTTGCTATCAGGCTGTGGTAAATAATCTGGAAGATGCTCATGAAATGATTGATACCGCAATTTCAACCGCCTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTGCTCATCCAACTTTTAGCCTGGATCCTGTTCCCTTCTCATTGTCTCCAAGATTGAGTAATCATTTGGGCTTAGAGGCTGCCGTGGAGGCGGCTGCAGAGTTCTTTAACAAGGCAGTGAAGCCGGTTATGGTAGGGGGGCCTAAACTTCGAGTTGCACATGCTGGCGATGCCTTTGTTGAACTAGCAGATGCTAGTGGTTATGCGCTCGCTGTCATGCCATCTGCAAAGGGCCTTGTGCCAGAGCACCACCCCCATTTCATTGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGCGCCGAGATTGTGGAGTCCGCAGATGCATACTTGTTTGCTGGACCGATTTTCAATGACTACAGCTCTGTTGGATACTCTCTGCTTCTCAAGAAAGAGAAGGCAATTGTGGTGCAGCCTGATCACGTGACCATAGCAAATGGCCCTTCATTTGGTTGTGTTCTCATGAAGGATTTCCTCCGAGCTCTGGCAAAGAGGCTCAAGCACAACAAAACTGCTCATGAGAACTACAGCAGGATCTTTGTTCCCAACGGACACCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAATGTTCTGTTCCACCACATCCAGAATATGCTGTCAAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGGTTGCGGGTATGAGTTCCAAATGCAGTATGGATCAATTGGTTGGTCAGTTGGAGCTACTCTTGGGTATGCTCAAGCTGTTACTGAGAAGCGTGTGATTGCTTTCATCGGCGATGGGAGTTTCCAGGTGACTGTTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGAACATCATCTTCCTGATAAACAACGGCGGATACACAATTGAGGTGGAGATCCATGACGGACCATACAATGTGATCAAGAACTGGAACTACACTGGACTAGTTGATGCCATCCACAACGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATTGAAGCGATTGAGACTGCAACAGGGGCGAAGAAGGATAGCTTGTGCTTCATTGAGGTGATAGCCCACAAGGACGATACCAGCAAAGAGTTGCTTGAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCCAGCCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

605

Amino Acids

64.67

Weight (kDa)

5.83

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	43 - 148	2e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	240 - 357	6.6e-22	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	451 - 575	6.1e-15	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	30
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	2 cut(s)	307, 371
AclWI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
AcoI	YGGCCR	1 cut(s)	136
AcvI	CACGTG	1 cut(s)	1057
AcyI	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
AfaI	GTAC	1 cut(s)	281
AfiI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
AflIII	ACRYGT	1 cut(s)	177
AgsI	TTSAA	6 cut(s)	576, 586, 815, 985, 1466, 1670
AhlI	ACTAGT	1 cut(s)	1597
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	4 cut(s)	190, 235, 654, 1452
AleI	CACNNNNGTG	1 cut(s)	176
AluBI	AGCT	9 cut(s)	93, 377, 618, 996, 1115, 1385, 1406, 1663, 1707
AluI	AGCT	9 cut(s)	93, 377, 618, 996, 1115, 1385, 1406, 1663, 1707
Alw21I	GWGCWC	4 cut(s)	47, 882, 923, 1117
Alw26I	GTCTC	3 cut(s)	684, 1277, 1673
Alw44I	GTGCAC	1 cut(s)	43
AlwI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
AlwNI	CAGNNNCTG	1 cut(s)	1315
AoxI	GGCC	4 cut(s)	136, 773, 865, 1072
ApaLI	GTGCAC	1 cut(s)	43
ApeKI	GCWGC	9 cut(s)	57, 255, 258, 284, 618, 713, 728, 1045, 1784
Asp700I	GAANNNNTTC	1 cut(s)	1103
AspLEI	GCGC	3 cut(s)	74, 841, 935
AspS9I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
AsuC2I	CCSGG	1 cut(s)	277
AsuHPI	GGTGA	5 cut(s)	206, 235, 446, 1468, 1735
AvaII	GGWCC	4 cut(s)	416, 974, 1559, 1645
BaeGI	GKGCMC	1 cut(s)	47
BalI	TGGCCA	1 cut(s)	138
BamHI	GGATCC	1 cut(s)	658
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	3 cut(s)	243, 351, 1117
BbrPI	CACGTG	1 cut(s)	1057
Bbv12I	GWGCWC	4 cut(s)	47, 882, 923, 1117
BbvI	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BccI	CCATC	3 cut(s)	862, 1436, 1616
BceAI	ACGGC	4 cut(s)	85, 307, 701, 1540
BcgI	CGANNNNNNTGC	4 cut(s)	34, 68, 254, 288
BciT130I	CCWGG	4 cut(s)	192, 237, 656, 1454
BciVI	GTATCC	2 cut(s)	998, 1523
BclI	TGATCA	2 cut(s)	1051, 1572
BcnI	CCSGG	1 cut(s)	277
BcoDI	GTCTC	3 cut(s)	684, 1277, 1673
BcuI	ACTAGT	1 cut(s)	1597
BfaI	CTAG	5 cut(s)	215, 818, 828, 1598, 1773
BfmI	CTRYAG	3 cut(s)	729, 991, 1159
BfoI	RGCGCY	1 cut(s)	75
BfuI	GTATCC	2 cut(s)	998, 1523
BglI	GCCNNNNNGGC	1 cut(s)	722
Bme1390I	CCNGG	5 cut(s)	192, 237, 277, 656, 1454
Bme18I	GGWCC	4 cut(s)	416, 974, 1559, 1645
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
BmiI	GGNNCC	4 cut(s)	73, 418, 660, 774
BmrFI	CCNGG	5 cut(s)	192, 237, 277, 656, 1454
BmrI	ACTGGG	1 cut(s)	916
BmsI	GCATC	5 cut(s)	535, 793, 814, 946, 1594
BmuI	ACTGGG	1 cut(s)	916
BpuEI	CTTGAG	4 cut(s)	1004, 1115, 1386, 1781
BpuMI	CCSGG	1 cut(s)	277
BsaAI	YACGTR	2 cut(s)	42, 1057
BsaHI	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
BsaJI	CCNNGG	4 cut(s)	190, 236, 717, 1641
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	1613, 1643
Bsc4I	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
Bse118I	RCCGGY	3 cut(s)	47, 757, 1324
Bse1I	ACTGG	3 cut(s)	911, 1586, 1597
Bse3DI	GCAATG	1 cut(s)	225
BseBI	CCWGG	4 cut(s)	192, 237, 656, 1454
BseDI	CCNNGG	4 cut(s)	190, 236, 717, 1641
BseGI	GGATG	3 cut(s)	640, 1242, 1608
BseLI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
BseMI	GCAATG	1 cut(s)	225
BseMII	CTCAG	3 cut(s)	364, 1272, 1404
BseNI	ACTGG	3 cut(s)	911, 1586, 1597
BseRI	GAGGAG	2 cut(s)	106, 1673
BseSI	GKGCMC	1 cut(s)	47
BseXI	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BseYI	CCCAGC	1 cut(s)	1802
BsgI	GTGCAG	2 cut(s)	1064, 1185
Bsh1236I	CGCG	2 cut(s)	307, 371
Bsh1285I	CGRYCG	1 cut(s)	305
BshFI	GGCC	4 cut(s)	138, 775, 867, 1074
BshNI	GGYRCC	1 cut(s)	71
BsiEI	CGRYCG	1 cut(s)	305
BsiHKAI	GWGCWC	4 cut(s)	47, 882, 923, 1117
BsiSI	CCGG	5 cut(s)	48, 276, 473, 758, 1325
BslFI	GGGAC	3 cut(s)	173, 451, 1304
BslI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
BsmAI	GTCTC	3 cut(s)	684, 1277, 1673
BsmFI	GGGAC	3 cut(s)	173, 451, 1304
BsnI	GGCC	4 cut(s)	138, 775, 867, 1074
Bsp1286I	GDGCHC	6 cut(s)	47, 243, 351, 882, 923, 1117
Bsp143I	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
BspANI	GGCC	4 cut(s)	138, 775, 867, 1074
BspCNI	CTCAG	3 cut(s)	363, 1273, 1405
BspFNI	CGCG	2 cut(s)	307, 371
BspHI	TCATGA	3 cut(s)	550, 1095, 1150
BspLI	GGNNCC	4 cut(s)	73, 418, 660, 774
BspMAI	CTGCAG	1 cut(s)	733
BspPI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
BspT107I	GGYRCC	1 cut(s)	71
BsrDI	GCAATG	1 cut(s)	225
BsrFI	RCCGGY	3 cut(s)	47, 757, 1324
BsrI	ACTGG	3 cut(s)	911, 1586, 1597
BssAI	RCCGGY	3 cut(s)	47, 757, 1324
BssECI	CCNNGG	4 cut(s)	190, 236, 717, 1641
BssMI	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
BssNI	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
BssT1I	CCWWGG	1 cut(s)	1641
Bst2UI	CCWGG	4 cut(s)	192, 237, 656, 1454
Bst4CI	ACNGT	5 cut(s)	187, 340, 383, 508, 1462
Bst6I	CTCTTC	1 cut(s)	1650
BstACI	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
BstAPI	GCANNNNNTGC	1 cut(s)	618
BstBAI	YACGTR	2 cut(s)	42, 1057
BstC8I	GCNNGC	6 cut(s)	35, 148, 596, 799, 843, 1326
BstDEI	CTNAG	5 cut(s)	350, 706, 1281, 1413, 1809
BstDSI	CCRYGG	1 cut(s)	717
BstEII	GGTNACC	1 cut(s)	468
BstF5I	GGATG	3 cut(s)	640, 1242, 1608
BstFNI	CGCG	2 cut(s)	307, 371
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	3 cut(s)	74, 841, 935
BstKTI	GATC	7 cut(s)	661, 1054, 1170, 1364, 1485, 1551, 1575
BstMAI	GTCTC	3 cut(s)	684, 1277, 1673
BstMBI	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
BstMCI	CGRYCG	1 cut(s)	305
BstNI	CCWGG	4 cut(s)	192, 237, 656, 1454
BstNSI	RCATGY	2 cut(s)	498, 797
BstPI	GGTNACC	1 cut(s)	468
BstSCI	CCNGG	5 cut(s)	190, 235, 275, 654, 1452
BstSFI	CTRYAG	3 cut(s)	729, 991, 1159
BstSLI	GKGCMC	1 cut(s)	47
BstUI	CGCG	2 cut(s)	307, 371
BstV1I	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BstX2I	RGATCY	3 cut(s)	658, 1167, 1548
BstXI	CCANNNNNNTGG	2 cut(s)	464, 896
BstYI	RGATCY	3 cut(s)	658, 1167, 1548
BsuI	GTATCC	2 cut(s)	998, 1523
BsuRI	GGCC	4 cut(s)	138, 775, 867, 1074
BtgI	CCRYGG	1 cut(s)	717
BtgZI	GCGATG	3 cut(s)	127, 816, 1455
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	3 cut(s)	640, 1242, 1608
BtsI	GCAGTG	2 cut(s)	756, 921
BtsIMutI	CAGTG	7 cut(s)	183, 336, 358, 388, 756, 921, 1590
Cac8I	GCNNGC	6 cut(s)	35, 148, 596, 799, 843, 1326
CaiI	CAGNNNCTG	1 cut(s)	1315
CciI	TCATGA	3 cut(s)	550, 1095, 1150
CfoI	GCGC	3 cut(s)	74, 841, 935
Cfr10I	RCCGGY	3 cut(s)	47, 757, 1324
Cfr13I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
CseI	GACGC	4 cut(s)	40, 131, 157, 448
Csp6I	GTAC	1 cut(s)	280
CspCI	CAANNNNNGTGG	2 cut(s)	1524, 1559
CviAII	CATG	9 cut(s)	495, 551, 794, 850, 1096, 1151, 1296, 1480, 1553
CviQI	GTAC	1 cut(s)	280
DdeI	CTNAG	5 cut(s)	350, 706, 1281, 1413, 1809
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	7 cut(s)	660, 1053, 1169, 1363, 1484, 1550, 1574
DpnII	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
EaeI	YGGCCR	1 cut(s)	136
Eam1104I	CTCTTC	1 cut(s)	1650
EarI	CTCTTC	1 cut(s)	1650
EciI	GGCGGA	1 cut(s)	1542
Ecl136II	GAGCTC	1 cut(s)	1115
Eco130I	CCWWGG	1 cut(s)	1641
Eco24I	GRGCYC	3 cut(s)	243, 351, 1117
Eco47I	GGWCC	4 cut(s)	416, 974, 1559, 1645
Eco53kI	GAGCTC	1 cut(s)	1115
Eco72I	CACGTG	1 cut(s)	1057
Eco91I	GGTNACC	1 cut(s)	468
EcoICRI	GAGCTC	1 cut(s)	1115
EcoO109I	RGGNCCY	3 cut(s)	416, 773, 865
EcoO65I	GGTNACC	1 cut(s)	468
EcoRII	CCWGG	4 cut(s)	190, 235, 654, 1452
EcoT14I	CCWWGG	1 cut(s)	1641
EcoT22I	ATGCAT	1 cut(s)	961
EcoT38I	GRGCYC	3 cut(s)	243, 351, 1117
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	1 cut(s)	1641
FaeI	CATG	9 cut(s)	498, 554, 797, 853, 1099, 1154, 1299, 1483, 1556
FalI	AAGNNNNNCTT	4 cut(s)	1691, 1723, 1743, 1775
FaqI	GGGAC	3 cut(s)	173, 451, 1304
FatI	CATG	9 cut(s)	494, 550, 793, 849, 1095, 1150, 1295, 1479, 1552
FauI	CCCGC	4 cut(s)	155, 300, 364, 1328
FbaI	TGATCA	2 cut(s)	1051, 1572
FokI	GGATG	3 cut(s)	627, 1229, 1595
FriOI	GRGCYC	3 cut(s)	243, 351, 1117
FspBI	CTAG	5 cut(s)	215, 818, 828, 1598, 1773
GlaI	GCGC	3 cut(s)	73, 840, 934
GsaI	CCCAGC	1 cut(s)	1806
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	4 cut(s)	138, 775, 867, 1074
HapII	CCGG	5 cut(s)	48, 276, 473, 758, 1325
HgaI	GACGC	4 cut(s)	40, 131, 157, 448
HhaI	GCGC	3 cut(s)	74, 841, 935
Hin1I	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
Hin1II	CATG	9 cut(s)	498, 554, 797, 853, 1099, 1154, 1299, 1483, 1556
Hin6I	GCGC	3 cut(s)	72, 839, 933
HinP1I	GCGC	3 cut(s)	72, 839, 933
HincII	GTYRAC	1 cut(s)	492
HindII	GTYRAC	1 cut(s)	492
HinfI	GANTC	5 cut(s)	312, 388, 448, 947, 1292
HpaII	CCGG	5 cut(s)	48, 276, 473, 758, 1325
HphI	GGTGA	5 cut(s)	206, 235, 446, 1468, 1735
Hpy166II	GTNNAC	4 cut(s)	45, 183, 492, 1476
Hpy188I	TCNGA	3 cut(s)	121, 1112, 1487
Hpy8I	GTNNAC	4 cut(s)	45, 183, 492, 1476
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	5 cut(s)	679, 1024, 1086, 1093, 1693
HpyCH4III	ACNGT	5 cut(s)	187, 340, 383, 508, 1462
HpyCH4IV	ACGT	4 cut(s)	27, 41, 177, 1056
HpyF3I	CTNAG	5 cut(s)	350, 706, 1281, 1413, 1809
HpySE526I	ACGT	4 cut(s)	27, 41, 177, 1056
Hsp92I	GRCGYC	5 cut(s)	27, 51, 72, 123, 168
Hsp92II	CATG	9 cut(s)	498, 554, 797, 853, 1099, 1154, 1299, 1483, 1556
HspAI	GCGC	3 cut(s)	72, 839, 933
KasI	GGCGCC	1 cut(s)	71
KroI	GCCGGC	1 cut(s)	1324
KroNI	GCCGGC	1 cut(s)	1326
Ksp22I	TGATCA	2 cut(s)	1051, 1572
Kzo9I	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
LmnI	GCTCC	3 cut(s)	374, 1382, 1660
Lsp1109I	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
LweI	GCATC	5 cut(s)	535, 793, 814, 946, 1594
MaeI	CTAG	5 cut(s)	215, 818, 828, 1598, 1773
MaeII	ACGT	4 cut(s)	27, 41, 177, 1056
MaeIII	GTNAC	8 cut(s)	169, 194, 328, 468, 508, 1057, 1408, 1456
MalI	GATC	7 cut(s)	660, 1053, 1169, 1363, 1484, 1550, 1574
MboI	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
MboII	GAAGA	6 cut(s)	443, 554, 1501, 1510, 1667, 1708
MfeI	CAATTG	3 cut(s)	1035, 1365, 1536
MflI	RGATCY	3 cut(s)	658, 1167, 1548
MhlI	GDGCHC	6 cut(s)	47, 243, 351, 882, 923, 1117
MlsI	TGGCCA	1 cut(s)	138
MluCI	AATT	7 cut(s)	12, 570, 633, 1035, 1319, 1365, 1536
MluNI	TGGCCA	1 cut(s)	138
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	4 cut(s)	321, 397, 956, 1286
MmeI	TCCRAC	4 cut(s)	391, 668, 982, 1360
Mox20I	TGGCCA	1 cut(s)	138
Mph1103I	ATGCAT	1 cut(s)	961
MroNI	GCCGGC	1 cut(s)	1324
MroXI	GAANNNNTTC	1 cut(s)	1103
MscI	TGGCCA	1 cut(s)	138
MseI	TTAA	5 cut(s)	201, 206, 741, 1224, 1302
MslI	CAYNNNNRTG	2 cut(s)	176, 1721
Msp20I	TGGCCA	1 cut(s)	138
MspI	CCGG	5 cut(s)	48, 276, 473, 758, 1325
MspR9I	CCNGG	5 cut(s)	192, 237, 277, 656, 1454
MunI	CAATTG	3 cut(s)	1035, 1365, 1536
MvaI	CCWGG	4 cut(s)	192, 237, 656, 1454
MvnI	CGCG	2 cut(s)	307, 371
NaeI	GCCGGC	1 cut(s)	1326
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	1 cut(s)	277
NdeII	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
NgoMIV	GCCGGC	1 cut(s)	1324
NlaIII	CATG	9 cut(s)	498, 554, 797, 853, 1099, 1154, 1299, 1483, 1556
NlaIV	GGNNCC	4 cut(s)	73, 418, 660, 774
NmeAIII	GCCGAG	2 cut(s)	256, 961
NmuCI	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
NsiI	ATGCAT	1 cut(s)	961
NspI	RCATGY	2 cut(s)	498, 797
OliI	CACNNNNGTG	1 cut(s)	176
PagI	TCATGA	3 cut(s)	550, 1095, 1150
PdiI	GCCGGC	1 cut(s)	1326
PdmI	GAANNNNTTC	1 cut(s)	1103
PfeI	GAWTC	1 cut(s)	448
PleI	GAGTC	4 cut(s)	320, 396, 955, 1286
PluTI	GGCGCC	1 cut(s)	75
PmaCI	CACGTG	1 cut(s)	1057
PmlI	CACGTG	1 cut(s)	1057
PpsI	GAGTC	4 cut(s)	320, 396, 955, 1286
Ppu21I	YACGTR	2 cut(s)	42, 1057
PpuMI	RGGWCCY	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	1117
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	4 cut(s)	190, 235, 654, 1452
PspCI	CACGTG	1 cut(s)	1057
PspEI	GGTNACC	1 cut(s)	468
PspFI	CCCAGC	1 cut(s)	1802
PspGI	CCWGG	4 cut(s)	190, 235, 654, 1452
PspN4I	GGNNCC	4 cut(s)	73, 418, 660, 774
PspPI	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
PspPPI	RGGWCCY	1 cut(s)	416
PsrI	GAACNNNNNNTAC	2 cut(s)	1571, 1603
PstI	CTGCAG	1 cut(s)	733
PstNI	CAGNNNCTG	1 cut(s)	1315
PsuI	RGATCY	3 cut(s)	658, 1167, 1548
RsaI	GTAC	1 cut(s)	281
RsaNI	GTAC	1 cut(s)	280
RseI	CAYNNNNRTG	2 cut(s)	176, 1721
SacI	GAGCTC	1 cut(s)	1117
SaqAI	TTAA	5 cut(s)	201, 206, 741, 1224, 1302
Sau3AI	GATC	7 cut(s)	658, 1051, 1167, 1361, 1482, 1548, 1572
Sau96I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
SchI	GAGTC	4 cut(s)	321, 397, 956, 1286
ScrFI	CCNGG	5 cut(s)	192, 237, 277, 656, 1454
SduI	GDGCHC	6 cut(s)	47, 243, 351, 882, 923, 1117
SfaNI	GCATC	5 cut(s)	535, 793, 814, 946, 1594
SfcI	CTRYAG	3 cut(s)	729, 991, 1159
SfoI	GGCGCC	1 cut(s)	73
SgrAI	CRCCGGYG	1 cut(s)	47
SinI	GGWCC	4 cut(s)	416, 974, 1559, 1645
SmiMI	CAYNNNNRTG	2 cut(s)	176, 1721
SmlI	CTYRAG	4 cut(s)	1019, 1130, 1401, 1760
SmoI	CTYRAG	4 cut(s)	1019, 1130, 1401, 1760
SpeI	ACTAGT	1 cut(s)	1597
Sse9I	AATT	7 cut(s)	12, 570, 633, 1035, 1319, 1365, 1536
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	5 cut(s)	215, 818, 828, 1598, 1773
SstI	GAGCTC	1 cut(s)	1117
StyD4I	CCNGG	5 cut(s)	190, 235, 275, 654, 1452
StyI	CCWWGG	1 cut(s)	1641
TaaI	ACNGT	5 cut(s)	187, 340, 383, 508, 1462
TaiI	ACGT	4 cut(s)	30, 44, 180, 1059
TaqI	TCGA	2 cut(s)	425, 784
TaqII	GACCGA	2 cut(s)	291, 991
TasI	AATT	7 cut(s)	12, 570, 633, 1035, 1319, 1365, 1536
TauI	GCSGC	3 cut(s)	77, 728, 1797
TfiI	GAWTC	1 cut(s)	448
Tru1I	TTAA	5 cut(s)	201, 206, 741, 1224, 1302
Tru9I	TTAA	5 cut(s)	201, 206, 741, 1224, 1302
TscAI	CASTG	7 cut(s)	190, 343, 358, 388, 756, 928, 1597
TseFI	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
TseI	GCWGC	9 cut(s)	57, 255, 258, 284, 618, 713, 728, 1045, 1784
Tsp45I	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
TspDTI	ATGAA	6 cut(s)	567, 883, 1068, 1112, 1423, 1705
TspGWI	ACGGA	4 cut(s)	188, 1197, 1572, 1637
TspRI	CASTG	7 cut(s)	190, 343, 358, 388, 756, 928, 1597
VneI	GTGCAC	1 cut(s)	43
VpaK11BI	GGWCC	4 cut(s)	416, 974, 1559, 1645
XceI	RCATGY	2 cut(s)	498, 797
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1355
XmnI	GAANNNNTTC	1 cut(s)	1103
XspI	CTAG	5 cut(s)	215, 818, 828, 1598, 1773
ZraI	GACGTC	1 cut(s)	28
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.