Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

25281200 .. 25282957

Gene Structure 1758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1172500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

ATGCCATCTGCAAAGGGGTTTGTACCAGAGCACCACCCCCATTTTATTGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGCGCCGAGATAGCCATTGTGGTGCAGCCTGATCGCGTGACCATAGCAAATGGCCCTTCATTTGGTTGTGTTCTTATGAAGGATTTCCTCAGAGCTCTTGCAAAGAAGCTCAGGCACAACAACACTGCTCATGAGAACTACCGCAGGATCTTTGTTCCCGATGGACACCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAATGTTCTGTTCCACCACATCCAGAATATGCTGTCAAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGATTGCGGGTATGAGTTCCAAATGCAATATGGATCAATCGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAGGCTGTTCCTCAGAAGCGTGTGATTGCTTTCATCGGTGACGGGAGTTTCCAGGTGACTGCTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGACCATCATCTTCCTGATAAACAACGGCGGGTACACAATTGAGGTGGAGATCCATGACGGACCCTACAATGTGATCAAGAACTGGAACTACACTGGACTGGTTGATGCCATCCACAATGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATCGAAGCGATTGAGACCGCAACCGGGGCAAAGAAGGATAGCTTGTGCTTCATAGAGGTGATAGTCCACAAGGATGATACCAGCAAAGAGTTGCTTGAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCGCCCAACCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

292

Amino Acids

32.26

Weight (kDa)

6.59

Isoelectric Point (pI)

30.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_C	PF02775	138 - 262	1.5e-16	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	117
AciI	CCGC	6 cut(s)	223, 396, 588, 744, 856, 860
AclWI	GGATC	4 cut(s)	236, 430, 538, 604
AfaI	GTAC	2 cut(s)	24, 593
AfiI	CCNNNNNNNGG	3 cut(s)	143, 679, 750
AjnI	CCWGG	1 cut(s)	513
AluBI	AGCT	4 cut(s)	176, 190, 724, 768
AluI	AGCT	4 cut(s)	176, 190, 724, 768
Alw21I	GWGCWC	3 cut(s)	33, 74, 178
Alw26I	GTCTC	2 cut(s)	338, 734
AlwI	GGATC	4 cut(s)	236, 430, 538, 604
AlwNI	CAGNNNCTG	1 cut(s)	376
AoxI	GGCC	1 cut(s)	133
ApeKI	GCWGC	2 cut(s)	106, 845
Asp700I	GAANNNNTTC	1 cut(s)	164
AspLEI	GCGC	1 cut(s)	86
AspS9I	GGNCC	3 cut(s)	134, 620, 706
AsuC2I	CCSGG	1 cut(s)	751
AsuHPI	GGTGA	3 cut(s)	512, 529, 796
AvaII	GGWCC	2 cut(s)	620, 706
BanII	GRGCYC	1 cut(s)	178
BbsI	GAAGAC	1 cut(s)	566
Bbv12I	GWGCWC	3 cut(s)	33, 74, 178
BbvI	GCAGC	2 cut(s)	118, 832
BccI	CCATC	4 cut(s)	13, 236, 572, 677
BceAI	ACGGC	1 cut(s)	601
BcgI	CGANNNNNNTGC	2 cut(s)	95, 129
BciT130I	CCWGG	1 cut(s)	515
BclI	TGATCA	1 cut(s)	633
BcnI	CCSGG	1 cut(s)	751
BcoDI	GTCTC	2 cut(s)	338, 734
BfaI	CTAG	1 cut(s)	834
BisI	GCNGC	3 cut(s)	107, 846, 856
BlsI	GCNGC	3 cut(s)	108, 847, 857
Bme1390I	CCNGG	2 cut(s)	515, 751
Bme18I	GGWCC	2 cut(s)	620, 706
BmgT120I	GGNCC	3 cut(s)	134, 620, 706
BmiI	GGNNCC	1 cut(s)	622
BmrFI	CCNGG	2 cut(s)	515, 751
BmrI	ACTGGG	1 cut(s)	67
BmsI	GCATC	1 cut(s)	655
BmuI	ACTGGG	1 cut(s)	67
BpiI	GAAGAC	1 cut(s)	566
Bpu10I	CCTNAGC	2 cut(s)	191, 462
BpuEI	CTTGAG	2 cut(s)	510, 842
BpuMI	CCSGG	1 cut(s)	751
BsaI	GGTCTC	1 cut(s)	734
BsaJI	CCNNGG	2 cut(s)	702, 750
BsaXI	ACNNNNNCTCC	2 cut(s)	674, 704
Bsc4I	CCNNNNNNNGG	3 cut(s)	143, 679, 750
Bse118I	RCCGGY	1 cut(s)	385
Bse1I	ACTGG	4 cut(s)	62, 647, 658, 663
BseBI	CCWGG	1 cut(s)	515
BseDI	CCNNGG	2 cut(s)	702, 750
BseGI	GGATG	3 cut(s)	303, 669, 805
BseLI	CCNNNNNNNGG	3 cut(s)	143, 679, 750
BseMII	CTCAG	5 cut(s)	184, 205, 333, 476, 488
BseNI	ACTGG	4 cut(s)	62, 647, 658, 663
BseRI	GAGGAG	1 cut(s)	734
BseXI	GCAGC	2 cut(s)	118, 832
BsgI	GTGCAG	2 cut(s)	125, 246
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	1 cut(s)	135
BsiHKAI	GWGCWC	3 cut(s)	33, 74, 178
BsiSI	CCGG	2 cut(s)	386, 750
BslFI	GGGAC	1 cut(s)	365
BslI	CCNNNNNNNGG	3 cut(s)	143, 679, 750
BsmAI	GTCTC	2 cut(s)	338, 734
BsmFI	GGGAC	1 cut(s)	365
BsnI	GGCC	1 cut(s)	135
Bso31I	GGTCTC	1 cut(s)	734
Bsp1286I	GDGCHC	3 cut(s)	33, 74, 178
Bsp143I	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
BspACI	CCGC	6 cut(s)	223, 396, 588, 744, 856, 860
BspANI	GGCC	1 cut(s)	135
BspCNI	CTCAG	5 cut(s)	183, 204, 334, 475, 487
BspFNI	CGCG	1 cut(s)	117
BspHI	TCATGA	1 cut(s)	211
BspLI	GGNNCC	1 cut(s)	622
BspPI	GGATC	4 cut(s)	236, 430, 538, 604
BspTNI	GGTCTC	1 cut(s)	734
BsrFI	RCCGGY	1 cut(s)	385
BsrI	ACTGG	4 cut(s)	62, 647, 658, 663
BssAI	RCCGGY	1 cut(s)	385
BssECI	CCNNGG	2 cut(s)	702, 750
BssMI	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
BssT1I	CCWWGG	1 cut(s)	702
Bst2UI	CCWGG	1 cut(s)	515
Bst6I	CTCTTC	1 cut(s)	711
BstC8I	GCNNGC	2 cut(s)	387, 860
BstDEI	CTNAG	6 cut(s)	170, 191, 342, 462, 474, 870
BstF5I	GGATG	3 cut(s)	303, 669, 805
BstFNI	CGCG	1 cut(s)	117
BstHHI	GCGC	1 cut(s)	86
BstKTI	GATC	7 cut(s)	115, 231, 425, 546, 612, 636, 729
BstMAI	GTCTC	2 cut(s)	338, 734
BstMBI	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
BstMWI	GCNNNNNNNGC	4 cut(s)	83, 92, 338, 752
BstNI	CCWGG	1 cut(s)	515
BstSCI	CCNGG	2 cut(s)	513, 749
BstUI	CGCG	1 cut(s)	117
BstV1I	GCAGC	2 cut(s)	118, 832
BstV2I	GAAGAC	1 cut(s)	566
BstX2I	RGATCY	2 cut(s)	228, 609
BstXI	CCANNNNNNTGG	1 cut(s)	47
BstYI	RGATCY	2 cut(s)	228, 609
BsuRI	GGCC	1 cut(s)	135
BtsCI	GGATG	3 cut(s)	303, 669, 805
BtsI	GCAGTG	2 cut(s)	72, 204
BtsIMutI	CAGTG	3 cut(s)	72, 204, 651
Cac8I	GCNNGC	2 cut(s)	387, 860
CaiI	CAGNNNCTG	1 cut(s)	376
CciI	TCATGA	1 cut(s)	211
CfoI	GCGC	1 cut(s)	86
Cfr10I	RCCGGY	1 cut(s)	385
Cfr13I	GGNCC	3 cut(s)	134, 620, 706
Csp6I	GTAC	2 cut(s)	23, 592
CspCI	CAANNNNNGTGG	2 cut(s)	585, 620
CviAII	CATG	4 cut(s)	212, 357, 541, 614
CviQI	GTAC	2 cut(s)	23, 592
DdeI	CTNAG	6 cut(s)	170, 191, 342, 462, 474, 870
DpnI	GATC	7 cut(s)	114, 230, 424, 545, 611, 635, 728
DpnII	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
Eam1104I	CTCTTC	1 cut(s)	711
EarI	CTCTTC	1 cut(s)	711
Ecl136II	GAGCTC	1 cut(s)	176
Eco130I	CCWWGG	1 cut(s)	702
Eco24I	GRGCYC	1 cut(s)	178
Eco31I	GGTCTC	1 cut(s)	734
Eco47I	GGWCC	2 cut(s)	620, 706
Eco53kI	GAGCTC	1 cut(s)	176
EcoICRI	GAGCTC	1 cut(s)	176
EcoRII	CCWGG	1 cut(s)	513
EcoT14I	CCWWGG	1 cut(s)	702
EcoT38I	GRGCYC	1 cut(s)	178
ErhI	CCWWGG	1 cut(s)	702
FaeI	CATG	4 cut(s)	215, 360, 544, 617
FalI	AAGNNNNNCTT	4 cut(s)	752, 784, 804, 836
FaqI	GGGAC	1 cut(s)	365
FatI	CATG	4 cut(s)	211, 356, 540, 613
FauI	CCCGC	3 cut(s)	389, 581, 867
FbaI	TGATCA	1 cut(s)	633
Fnu4HI	GCNGC	3 cut(s)	107, 846, 856
FokI	GGATG	3 cut(s)	290, 656, 812
FriOI	GRGCYC	1 cut(s)	178
Fsp4HI	GCNGC	3 cut(s)	107, 846, 856
FspBI	CTAG	1 cut(s)	834
GlaI	GCGC	1 cut(s)	85
GluI	GCNGC	3 cut(s)	107, 846, 856
HaeIII	GGCC	1 cut(s)	135
HapII	CCGG	2 cut(s)	386, 750
HhaI	GCGC	1 cut(s)	86
Hin1II	CATG	4 cut(s)	215, 360, 544, 617
Hin6I	GCGC	1 cut(s)	84
HinP1I	GCGC	1 cut(s)	84
HinfI	GANTC	1 cut(s)	353
HpaII	CCGG	2 cut(s)	386, 750
HphI	GGTGA	3 cut(s)	512, 529, 796
Hpy166II	GTNNAC	3 cut(s)	537, 594, 793
Hpy188I	TCNGA	3 cut(s)	173, 477, 548
Hpy188III	TCNNGA	6 cut(s)	212, 239, 307, 527, 574, 637
Hpy8I	GTNNAC	3 cut(s)	537, 594, 793
HpyAV	CCTTC	3 cut(s)	147, 154, 754
HpyCH4V	TGCA	5 cut(s)	11, 106, 182, 263, 415
HpyF10VI	GCNNNNNNNGC	4 cut(s)	83, 92, 338, 752
HpyF3I	CTNAG	6 cut(s)	170, 191, 342, 462, 474, 870
Hsp92II	CATG	4 cut(s)	215, 360, 544, 617
HspAI	GCGC	1 cut(s)	84
KroI	GCCGGC	1 cut(s)	385
KroNI	GCCGGC	1 cut(s)	387
Ksp22I	TGATCA	1 cut(s)	633
Kzo9I	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
LmnI	GCTCC	2 cut(s)	443, 721
Lsp1109I	GCAGC	2 cut(s)	118, 832
LweI	GCATC	1 cut(s)	655
MaeI	CTAG	1 cut(s)	834
MaeIII	GTNAC	3 cut(s)	118, 500, 517
MalI	GATC	7 cut(s)	114, 230, 424, 545, 611, 635, 728
MboI	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
MboII	GAAGA	3 cut(s)	562, 571, 728
MfeI	CAATTG	1 cut(s)	597
MflI	RGATCY	2 cut(s)	228, 609
MhlI	GDGCHC	3 cut(s)	33, 74, 178
MluCI	AATT	2 cut(s)	380, 597
MlyI	GAGTC	1 cut(s)	347
MmeI	TCCRAC	1 cut(s)	421
MnlI	CCTC	8 cut(s)	179, 261, 273, 483, 595, 676, 712, 775
MroNI	GCCGGC	1 cut(s)	385
MroXI	GAANNNNTTC	1 cut(s)	164
MseI	TTAA	2 cut(s)	285, 363
MslI	CAYNNNNRTG	3 cut(s)	101, 782, 798
MspI	CCGG	2 cut(s)	386, 750
MspR9I	CCNGG	2 cut(s)	515, 751
MunI	CAATTG	1 cut(s)	597
MvaI	CCWGG	1 cut(s)	515
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	4 cut(s)	83, 92, 338, 752
NaeI	GCCGGC	1 cut(s)	387
NciI	CCSGG	1 cut(s)	751
NdeII	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
NgoMIV	GCCGGC	1 cut(s)	385
NlaIII	CATG	4 cut(s)	215, 360, 544, 617
NlaIV	GGNNCC	1 cut(s)	622
NmeAIII	GCCGAG	1 cut(s)	112
NmuCI	GTSAC	3 cut(s)	118, 500, 517
PagI	TCATGA	1 cut(s)	211
PdiI	GCCGGC	1 cut(s)	387
PdmI	GAANNNNTTC	1 cut(s)	164
PkrI	GCNGC	3 cut(s)	108, 847, 857
PleI	GAGTC	1 cut(s)	347
PpsI	GAGTC	1 cut(s)	347
Psp124BI	GAGCTC	1 cut(s)	178
Psp6I	CCWGG	1 cut(s)	513
PspGI	CCWGG	1 cut(s)	513
PspN4I	GGNNCC	1 cut(s)	622
PspPI	GGNCC	3 cut(s)	134, 620, 706
PsrI	GAACNNNNNNTAC	2 cut(s)	632, 664
PstNI	CAGNNNCTG	1 cut(s)	376
PsuI	RGATCY	2 cut(s)	228, 609
RsaI	GTAC	2 cut(s)	24, 593
RsaNI	GTAC	2 cut(s)	23, 592
RseI	CAYNNNNRTG	3 cut(s)	101, 782, 798
SacI	GAGCTC	1 cut(s)	178
SaqAI	TTAA	2 cut(s)	285, 363
SatI	GCNGC	3 cut(s)	107, 846, 856
Sau3AI	GATC	7 cut(s)	112, 228, 422, 543, 609, 633, 726
Sau96I	GGNCC	3 cut(s)	134, 620, 706
SchI	GAGTC	1 cut(s)	347
ScrFI	CCNGG	2 cut(s)	515, 751
SduI	GDGCHC	3 cut(s)	33, 74, 178
SetI	ASST	9 cut(s)	178, 192, 277, 519, 606, 708, 726, 770, 786
SfaNI	GCATC	1 cut(s)	655
SinI	GGWCC	2 cut(s)	620, 706
SmiMI	CAYNNNNRTG	3 cut(s)	101, 782, 798
SmlI	CTYRAG	2 cut(s)	525, 821
SmoI	CTYRAG	2 cut(s)	525, 821
Sse9I	AATT	2 cut(s)	380, 597
SsiI	CCGC	6 cut(s)	223, 396, 588, 744, 856, 860
SspMI	CTAG	1 cut(s)	834
SstI	GAGCTC	1 cut(s)	178
StyD4I	CCNGG	2 cut(s)	513, 749
StyI	CCWWGG	1 cut(s)	702
TaqI	TCGA	1 cut(s)	729
TasI	AATT	2 cut(s)	380, 597
TauI	GCSGC	1 cut(s)	858
Tru1I	TTAA	2 cut(s)	285, 363
Tru9I	TTAA	2 cut(s)	285, 363
TscAI	CASTG	3 cut(s)	79, 211, 658
TseFI	GTSAC	3 cut(s)	118, 500, 517
TseI	GCWGC	2 cut(s)	106, 845
Tsp45I	GTSAC	3 cut(s)	118, 500, 517
TspDTI	ATGAA	4 cut(s)	129, 173, 484, 766
TspGWI	ACGGA	2 cut(s)	633, 698
TspRI	CASTG	3 cut(s)	79, 211, 658
VpaK11BI	GGWCC	2 cut(s)	620, 706
XcmI	CCANNNNNNNNNTGG	1 cut(s)	416
XmnI	GAANNNNTTC	1 cut(s)	164
XspI	CTAG	1 cut(s)	834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.