Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

6634937 .. 6639405

Gene Structure 4469 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g11100.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGACACCAAGATTGGCTCCATCGACGTTTGCAAAGCCGAGAGCCACGACGTTGGTTGTTTACCAAACGGCGCCACCTCCACCGTCCAAAACTCCGTCCCCTCCACCACCCTCAGCTCCGCCGATGCCACCCTCGGCCGCCATCTGGCACGCCGCCTCGTCCAAATCGGCGTCACCGACGTCTTCACCGTCCCCGGCGACTTCAACTTGACCCTTCTCGACCACCTCGTCGCCGAGCCCGGCCTCACCAACATTGGCTGCTGCAATGAGCTCAACGCCGGGTACGCCGCCGACGGCTACGCGCGGTCGCGTGGCGTCGGCGCGTGCGTGGTGACTTTCACTGTGGGTGGACTGAGTGTGCTGAACGCGATCGCCGGTGCGTGTAGTGAGAATTTGCCGGTGATTTGTATTGTTGGTGGGCCCAACTCTAATGATTATGGGACTAACCGGATTCTTCACCATACTATTGGGTTGCCGGACTTCAGTCAAGAGCTCCGTTGCTTCCAGACCGTGACTTGCTTTCAGGCTGTGGTGAATAATCTGGAGGATGCGCATGAGTTGATTGATACCGCAATTTCGACTGCGTTGAAAGAAAGCAAGCCTGTGTATATCAGCATTGGCTGCAACTTGGCTGGGATTCCTCATCCTACTTTCAGCCGTGAACCTGTTCCATTTTCATTGTCTCCAAAATTGAGCAATAAGTGGGGACTAGAGGCTGCAGTGGAGGCTGCTGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTGGGCGGGCCCAAACTGCGCTCTGCACATGCCGGTGATGCCTTTGTTGAACTGGCTGATGCTTCTGGATTTGCTCTGGCTGTGATGCCATCAGCAAAGGGGCAAGTGCCAGAGCACCATCCCCGTTTTATCGGAACATACTGGGGCGCTGTGAGCACTGCCTTTTGTGCTGAGATTGTGGAGTCTGCAGATGCATACTTGTTTGCTGGGCCGATTTTCAATGATTACAGCTCTGTTGGATACTCACTCCTTCTCAAGAAAGAGAAGGCAATCATTGTGCAGCCTGATCGTGTGACAATAGGAAATGGCCCTACATTTGGTTGTGTTCTTATGAAGGATTTCCTCGTAGGCCTAGCAAAGAAGCTGAAGCATAATAACACTGCTTATGAGAACTACCGCAGGATCTTTGTGCCTGATGGCCACCCTCTGAAGGCCGCACCCAAAGAACCTTTGAGGGTTAATGTTCTGTTCAAACACATTCAGAAGATGCTGTCAGCTGAAACCGCTGTGATTGCCGAGACAGGGGACTCATGGTTTAACTGTCAGAAGCTGAAATTGCCACCCGGCTGCGGGTATGAGTTCCAAATGCAATATGGATCAATTGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAGGCTGTACCTGAGAAGCGAGTGATTGCTTTCATTGGTGATGGGAGCTTCCAGGTGACTGCTCAAGATGTGTCCACAATGATTCGAAATGGACAGAGGACCATCATTTTCCTGATAAACAATGGTGGATACACCATTGAAGTGGAAATCCATGATGGACCATACAATGTGATCAAGAACTGGAACTACACTGGACTGGTTGATGCAATCCACAATGGGGAAGGCAAGTGCTGGACAACCAAGGTGCGTTGCGAAGAGGAGCTGATTGAAGCAATAGAGACTGCAAATGGACCCAAGAAGGATAGCTTCTGCTTCATTGAGGTGATTGTTCACAAGGATGATACCAGCAAAGAGTTGCTTGAGTGGGGGTCTAGGGTTTCGGCTGCCAACAGCCGCCCACCTAATCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

606

Amino Acids

65.11

Weight (kDa)

5.91

Isoelectric Point (pI)

28.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	44 - 149	1.4e-28	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	241 - 359	2.7e-22	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	451 - 576	9.5e-16	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	227
AatII	GACGTC	1 cut(s)	183
Acc16I	TGCGCA	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	5 cut(s)	302, 304, 310, 323, 368
AclWI	GGATC	2 cut(s)	1178, 1372
AcoI	YGGCCR	2 cut(s)	136, 1186
AcsI	RAATTY	1 cut(s)	391
AcuI	CTGAAG	3 cut(s)	466, 1154, 1217
AcyI	GRCGYC	4 cut(s)	72, 171, 180, 315
AfaI	GTAC	2 cut(s)	284, 1413
AfiI	CCNNNNNNNGG	7 cut(s)	145, 1085, 1198, 1290, 1337, 1338, 1621
AgsI	TTSAA	8 cut(s)	205, 589, 745, 818, 988, 1240, 1544, 1673
AjnI	CCWGG	1 cut(s)	1455
Alw21I	GWGCWC	4 cut(s)	273, 495, 885, 926
Alw26I	GTCTC	3 cut(s)	687, 1280, 1676
AlwI	GGATC	2 cut(s)	1178, 1372
AlwNI	CAGNNNCTG	1 cut(s)	1318
AoxI	GGCC	9 cut(s)	136, 241, 419, 776, 977, 1075, 1117, 1186, 1200
ApaI	GGGCCC	2 cut(s)	423, 780
ApeKI	GCWGC	9 cut(s)	258, 261, 621, 716, 728, 731, 1048, 1335, 1787
ApoI	RAATTY	1 cut(s)	391
AsiSI	GCGATCGC	1 cut(s)	372
Asp700I	GAANNNNTTC	2 cut(s)	666, 1106
AspLEI	GCGC	6 cut(s)	74, 304, 323, 553, 789, 917
AspS9I	GGNCC	9 cut(s)	419, 420, 776, 777, 977, 1076, 1503, 1562, 1694
AsuC2I	CCSGG	4 cut(s)	195, 240, 280, 1332
AsuII	TTCGAA	1 cut(s)	1489
AvaII	GGWCC	3 cut(s)	1503, 1562, 1694
BaeGI	GKGCMC	2 cut(s)	423, 780
BalI	TGGCCA	1 cut(s)	1188
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	5 cut(s)	240, 273, 423, 495, 780
BbsI	GAAGAC	1 cut(s)	175
Bbv12I	GWGCWC	4 cut(s)	273, 495, 885, 926
BbvCI	CCTCAGC	1 cut(s)	113
BbvI	GCAGC	9 cut(s)	245, 248, 608, 703, 715, 718, 1060, 1322, 1774
BccI	CCATC	8 cut(s)	29, 150, 865, 894, 1178, 1439, 1514, 1553
BceAI	ACGGC	3 cut(s)	85, 310, 642
BcgI	CGANNNNNNTGC	2 cut(s)	1037, 1071
BciT130I	CCWGG	1 cut(s)	1457
BciVI	GTATCC	2 cut(s)	1001, 1526
BclI	TGATCA	1 cut(s)	1575
BcnI	CCSGG	4 cut(s)	195, 240, 280, 1332
BcoDI	GTCTC	3 cut(s)	687, 1280, 1676
BfaI	CTAG	3 cut(s)	710, 1121, 1776
BfmI	CTRYAG	3 cut(s)	717, 732, 954
BfoI	RGCGCY	2 cut(s)	75, 918
BfuI	GTATCC	2 cut(s)	1001, 1526
Bme1390I	CCNGG	5 cut(s)	195, 240, 280, 1332, 1457
Bme18I	GGWCC	3 cut(s)	1503, 1562, 1694
BmgT120I	GGNCC	9 cut(s)	419, 420, 776, 777, 977, 1076, 1503, 1562, 1694
BmiI	GGNNCC	5 cut(s)	19, 73, 421, 778, 1696
BmrFI	CCNGG	5 cut(s)	195, 240, 280, 1332, 1457
BmrI	ACTGGG	1 cut(s)	919
BmsI	GCATC	8 cut(s)	115, 538, 796, 817, 843, 949, 1245, 1597
BmuI	ACTGGG	1 cut(s)	919
BoxI	GACNNNNGTC	1 cut(s)	483
BpiI	GAAGAC	1 cut(s)	175
BpmI	CTGGAG	1 cut(s)	563
Bpu10I	CCTNAGC	2 cut(s)	113, 1404
Bpu14I	TTCGAA	1 cut(s)	1489
BpuEI	CTTGAG	3 cut(s)	1007, 1452, 1784
BpuMI	CCSGG	4 cut(s)	195, 240, 280, 1332
BsaHI	GRCGYC	4 cut(s)	72, 171, 180, 315
BsaJI	CCNNGG	3 cut(s)	133, 193, 1644
BsaWI	WCCGGW	1 cut(s)	447
Bsc4I	CCNNNNNNNGG	7 cut(s)	145, 1085, 1198, 1290, 1337, 1338, 1621
Bse118I	RCCGGY	4 cut(s)	374, 397, 760, 800
Bse1I	ACTGG	5 cut(s)	825, 914, 1589, 1600, 1605
Bse3DI	GCAATG	1 cut(s)	271
BseBI	CCWGG	1 cut(s)	1457
BseDI	CCNNGG	3 cut(s)	133, 193, 1644
BseGI	GGATG	4 cut(s)	553, 643, 886, 1747
BseLI	CCNNNNNNNGG	7 cut(s)	145, 1085, 1198, 1290, 1337, 1338, 1621
BseMI	GCAATG	1 cut(s)	271
BseMII	CTCAG	5 cut(s)	127, 344, 930, 1407, 1418
BseNI	ACTGG	5 cut(s)	825, 914, 1589, 1600, 1605
BseRI	GAGGAG	1 cut(s)	1676
BseSI	GKGCMC	2 cut(s)	423, 780
BseX3I	CGGCCG	1 cut(s)	136
BseXI	GCAGC	9 cut(s)	245, 248, 608, 703, 715, 718, 1060, 1322, 1774
BseYI	CCCAGC	2 cut(s)	632, 974
BsgI	GTGCAG	2 cut(s)	777, 1067
Bsh1236I	CGCG	5 cut(s)	302, 304, 310, 323, 368
Bsh1285I	CGRYCG	3 cut(s)	139, 308, 372
BshFI	GGCC	9 cut(s)	138, 243, 421, 778, 979, 1077, 1119, 1188, 1202
BshNI	GGYRCC	1 cut(s)	71
BsiEI	CGRYCG	3 cut(s)	139, 308, 372
BsiHKAI	GWGCWC	4 cut(s)	273, 495, 885, 926
BslFI	GGGAC	5 cut(s)	83, 176, 454, 720, 1307
BslI	CCNNNNNNNGG	7 cut(s)	145, 1085, 1198, 1290, 1337, 1338, 1621
BsmAI	GTCTC	3 cut(s)	687, 1280, 1676
BsmFI	GGGAC	5 cut(s)	83, 176, 454, 720, 1307
BsnI	GGCC	9 cut(s)	138, 243, 421, 778, 979, 1077, 1119, 1188, 1202
Bsp119I	TTCGAA	1 cut(s)	1489
Bsp120I	GGGCCC	2 cut(s)	419, 776
Bsp1286I	GDGCHC	7 cut(s)	240, 273, 423, 495, 780, 885, 926
Bsp143I	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
BspANI	GGCC	9 cut(s)	138, 243, 421, 778, 979, 1077, 1119, 1188, 1202
BspCNI	CTCAG	5 cut(s)	126, 345, 931, 1408, 1417
BspFNI	CGCG	5 cut(s)	302, 304, 310, 323, 368
BspLI	GGNNCC	5 cut(s)	19, 73, 421, 778, 1696
BspMAI	CTGCAG	3 cut(s)	721, 736, 958
BspPI	GGATC	2 cut(s)	1178, 1372
BspT104I	TTCGAA	1 cut(s)	1489
BspT107I	GGYRCC	1 cut(s)	71
BsrDI	GCAATG	1 cut(s)	271
BsrFI	RCCGGY	4 cut(s)	374, 397, 760, 800
BsrI	ACTGG	5 cut(s)	825, 914, 1589, 1600, 1605
BssAI	RCCGGY	4 cut(s)	374, 397, 760, 800
BssECI	CCNNGG	3 cut(s)	133, 193, 1644
BssMI	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
BssNI	GRCGYC	4 cut(s)	72, 171, 180, 315
BssT1I	CCWWGG	1 cut(s)	1644
Bst2UI	CCWGG	1 cut(s)	1457
Bst4CI	ACNGT	5 cut(s)	85, 190, 343, 511, 1310
Bst6I	CTCTTC	1 cut(s)	1653
BstACI	GRCGYC	4 cut(s)	72, 171, 180, 315
BstAPI	GCANNNNNTGC	1 cut(s)	621
BstBI	TTCGAA	1 cut(s)	1489
BstC8I	GCNNGC	4 cut(s)	151, 325, 599, 776
BstDEI	CTNAG	6 cut(s)	113, 353, 939, 1404, 1416, 1812
BstF5I	GGATG	4 cut(s)	553, 643, 886, 1747
BstFNI	CGCG	5 cut(s)	302, 304, 310, 323, 368
BstH2I	RGCGCY	2 cut(s)	75, 918
BstHHI	GCGC	6 cut(s)	74, 304, 323, 553, 789, 917
BstKTI	GATC	5 cut(s)	372, 1057, 1173, 1367, 1578
BstMAI	GTCTC	3 cut(s)	687, 1280, 1676
BstMBI	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
BstMCI	CGRYCG	3 cut(s)	139, 308, 372
BstNI	CCWGG	1 cut(s)	1457
BstNSI	RCATGY	1 cut(s)	800
BstPAI	GACNNNNGTC	1 cut(s)	483
BstSCI	CCNGG	5 cut(s)	193, 238, 278, 1330, 1455
BstSFI	CTRYAG	3 cut(s)	717, 732, 954
BstSLI	GKGCMC	2 cut(s)	423, 780
BstUI	CGCG	5 cut(s)	302, 304, 310, 323, 368
BstV1I	GCAGC	9 cut(s)	245, 248, 608, 703, 715, 718, 1060, 1322, 1774
BstV2I	GAAGAC	1 cut(s)	175
BstX2I	RGATCY	1 cut(s)	1170
BstXI	CCANNNNNNTGG	3 cut(s)	53, 467, 1546
BstYI	RGATCY	1 cut(s)	1170
BstZI	CGGCCG	1 cut(s)	136
BsuI	GTATCC	2 cut(s)	1001, 1526
BsuRI	GGCC	9 cut(s)	138, 243, 421, 778, 979, 1077, 1119, 1188, 1202
BtsCI	GGATG	4 cut(s)	553, 643, 886, 1747
BtsI	GCAGTG	4 cut(s)	726, 759, 924, 1146
BtsIMutI	CAGTG	6 cut(s)	339, 726, 759, 924, 1146, 1593
Cac8I	GCNNGC	4 cut(s)	151, 325, 599, 776
CaiI	CAGNNNCTG	1 cut(s)	1318
CfoI	GCGC	6 cut(s)	74, 304, 323, 553, 789, 917
Cfr10I	RCCGGY	4 cut(s)	374, 397, 760, 800
Cfr13I	GGNCC	9 cut(s)	419, 420, 776, 777, 977, 1076, 1503, 1562, 1694
CseI	GACGC	2 cut(s)	160, 304
Csp6I	GTAC	2 cut(s)	283, 1412
CviAII	CATG	4 cut(s)	554, 797, 1299, 1556
CviQI	GTAC	2 cut(s)	283, 1412
DdeI	CTNAG	6 cut(s)	113, 353, 939, 1404, 1416, 1812
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	5 cut(s)	371, 1056, 1172, 1366, 1577
DpnII	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
DrdI	GACNNNNNNGTC	1 cut(s)	227
DseDI	GACNNNNNNGTC	1 cut(s)	227
EaeI	YGGCCR	2 cut(s)	136, 1186
EagI	CGGCCG	1 cut(s)	136
Eam1104I	CTCTTC	1 cut(s)	1653
EarI	CTCTTC	1 cut(s)	1653
EciI	GGCGGA	1 cut(s)	109
Ecl136II	GAGCTC	2 cut(s)	271, 493
EclXI	CGGCCG	1 cut(s)	136
Eco130I	CCWWGG	1 cut(s)	1644
Eco147I	AGGCCT	1 cut(s)	1119
Eco24I	GRGCYC	5 cut(s)	240, 273, 423, 495, 780
Eco47I	GGWCC	3 cut(s)	1503, 1562, 1694
Eco52I	CGGCCG	1 cut(s)	136
Eco53kI	GAGCTC	2 cut(s)	271, 493
Eco57I	CTGAAG	3 cut(s)	466, 1154, 1217
EcoICRI	GAGCTC	2 cut(s)	271, 493
EcoRII	CCWGG	1 cut(s)	1455
EcoT14I	CCWWGG	1 cut(s)	1644
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	5 cut(s)	240, 273, 423, 495, 780
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	1 cut(s)	1644
FaeI	CATG	4 cut(s)	557, 800, 1302, 1559
FalI	AAGNNNNNCTT	4 cut(s)	1694, 1726, 1746, 1778
FaqI	GGGAC	5 cut(s)	83, 176, 454, 720, 1307
FatI	CATG	4 cut(s)	553, 796, 1298, 1555
FauI	CCCGC	2 cut(s)	767, 1331
FbaI	TGATCA	1 cut(s)	1575
FokI	GGATG	4 cut(s)	560, 630, 873, 1754
FriOI	GRGCYC	5 cut(s)	240, 273, 423, 495, 780
FspAI	RTGCGCAY	1 cut(s)	552
FspBI	CTAG	3 cut(s)	710, 1121, 1776
FspI	TGCGCA	1 cut(s)	552
GlaI	GCGC	6 cut(s)	73, 303, 322, 552, 788, 916
GsaI	CCCAGC	2 cut(s)	636, 978
GsuI	CTGGAG	1 cut(s)	563
HaeII	RGCGCY	2 cut(s)	75, 918
HaeIII	GGCC	9 cut(s)	138, 243, 421, 778, 979, 1077, 1119, 1188, 1202
HgaI	GACGC	2 cut(s)	160, 304
HhaI	GCGC	6 cut(s)	74, 304, 323, 553, 789, 917
Hin1I	GRCGYC	4 cut(s)	72, 171, 180, 315
Hin1II	CATG	4 cut(s)	557, 800, 1302, 1559
Hin6I	GCGC	6 cut(s)	72, 302, 321, 551, 787, 915
HinP1I	GCGC	6 cut(s)	72, 302, 321, 551, 787, 915
HinfI	GANTC	5 cut(s)	451, 637, 950, 1295, 1486
Hpy166II	GTNNAC	5 cut(s)	62, 350, 662, 1479, 1735
Hpy188I	TCNGA	4 cut(s)	902, 1197, 1251, 1314
Hpy8I	GTNNAC	5 cut(s)	62, 350, 662, 1479, 1735
Hpy99I	CGWCG	6 cut(s)	29, 53, 182, 233, 296, 320
HpyAV	CCTTC	7 cut(s)	224, 1027, 1028, 1096, 1192, 1619, 1696
HpyCH4III	ACNGT	5 cut(s)	85, 190, 343, 511, 1310
HpyCH4IV	ACGT	3 cut(s)	27, 51, 180
HpyF3I	CTNAG	6 cut(s)	113, 353, 939, 1404, 1416, 1812
HpySE526I	ACGT	3 cut(s)	27, 51, 180
Hsp92I	GRCGYC	4 cut(s)	72, 171, 180, 315
Hsp92II	CATG	4 cut(s)	557, 800, 1302, 1559
HspAI	GCGC	6 cut(s)	72, 302, 321, 551, 787, 915
KasI	GGCGCC	1 cut(s)	71
Ksp22I	TGATCA	1 cut(s)	1575
Kzo9I	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
LmnI	GCTCC	6 cut(s)	23, 122, 498, 1385, 1449, 1663
Lsp1109I	GCAGC	9 cut(s)	245, 248, 608, 703, 715, 718, 1060, 1322, 1774
LweI	GCATC	8 cut(s)	115, 538, 796, 817, 843, 949, 1245, 1597
MaeI	CTAG	3 cut(s)	710, 1121, 1776
MaeII	ACGT	3 cut(s)	27, 51, 180
MaeIII	GTNAC	5 cut(s)	172, 331, 511, 1060, 1459
MalI	GATC	5 cut(s)	371, 1056, 1172, 1366, 1577
MboI	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
MboII	GAAGA	4 cut(s)	175, 446, 1264, 1670
MfeI	CAATTG	1 cut(s)	1368
MflI	RGATCY	1 cut(s)	1170
MhlI	GDGCHC	7 cut(s)	240, 273, 423, 495, 780, 885, 926
MlsI	TGGCCA	1 cut(s)	1188
MluCI	AATT	5 cut(s)	391, 573, 689, 1322, 1368
MluNI	TGGCCA	1 cut(s)	1188
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	2 cut(s)	959, 1289
MmeI	TCCRAC	2 cut(s)	985, 1363
Mox20I	TGGCCA	1 cut(s)	1188
Mph1103I	ATGCAT	1 cut(s)	964
MroXI	GAANNNNTTC	2 cut(s)	666, 1106
MscI	TGGCCA	1 cut(s)	1188
MseI	TTAA	2 cut(s)	1227, 1305
MslI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
Msp20I	TGGCCA	1 cut(s)	1188
MspA1I	CMGCKG	2 cut(s)	1265, 1274
MspR9I	CCNGG	5 cut(s)	195, 240, 280, 1332, 1457
MunI	CAATTG	1 cut(s)	1368
MvaI	CCWGG	1 cut(s)	1457
MvnI	CGCG	5 cut(s)	302, 304, 310, 323, 368
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	4 cut(s)	195, 240, 280, 1332
NdeII	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
NlaIII	CATG	4 cut(s)	557, 800, 1302, 1559
NlaIV	GGNNCC	5 cut(s)	19, 73, 421, 778, 1696
NmeAIII	GCCGAG	4 cut(s)	64, 114, 259, 1309
NmuCI	GTSAC	5 cut(s)	172, 331, 511, 1060, 1459
NsbI	TGCGCA	1 cut(s)	552
NsiI	ATGCAT	1 cut(s)	964
NspI	RCATGY	1 cut(s)	800
NspV	TTCGAA	1 cut(s)	1489
PceI	AGGCCT	1 cut(s)	1119
PcsI	WCGNNNNNNNCGW	4 cut(s)	174, 186, 225, 324
PdmI	GAANNNNTTC	2 cut(s)	666, 1106
PfeI	GAWTC	3 cut(s)	451, 637, 1486
Ple19I	CGATCG	1 cut(s)	372
PleI	GAGTC	2 cut(s)	958, 1289
PluTI	GGCGCC	1 cut(s)	75
PpsI	GAGTC	2 cut(s)	958, 1289
PshAI	GACNNNNGTC	1 cut(s)	483
Psp124BI	GAGCTC	2 cut(s)	273, 495
Psp6I	CCWGG	1 cut(s)	1455
PspFI	CCCAGC	2 cut(s)	632, 974
PspGI	CCWGG	1 cut(s)	1455
PspN4I	GGNNCC	5 cut(s)	19, 73, 421, 778, 1696
PspOMI	GGGCCC	2 cut(s)	419, 776
PspPI	GGNCC	9 cut(s)	419, 420, 776, 777, 977, 1076, 1503, 1562, 1694
PsrI	GAACNNNNNNTAC	2 cut(s)	1574, 1606
PstI	CTGCAG	3 cut(s)	721, 736, 958
PstNI	CAGNNNCTG	1 cut(s)	1318
PsuI	RGATCY	1 cut(s)	1170
PvuI	CGATCG	1 cut(s)	372
PvuII	CAGCTG	1 cut(s)	1265
RgaI	GCGATCGC	1 cut(s)	372
RsaI	GTAC	2 cut(s)	284, 1413
RsaNI	GTAC	2 cut(s)	283, 1412
RseI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
SacI	GAGCTC	2 cut(s)	273, 495
SaqAI	TTAA	2 cut(s)	1227, 1305
Sau3AI	GATC	5 cut(s)	369, 1054, 1170, 1364, 1575
Sau96I	GGNCC	9 cut(s)	419, 420, 776, 777, 977, 1076, 1503, 1562, 1694
SchI	GAGTC	2 cut(s)	959, 1289
ScrFI	CCNGG	5 cut(s)	195, 240, 280, 1332, 1457
SduI	GDGCHC	7 cut(s)	240, 273, 423, 495, 780, 885, 926
SfaAI	GCGATCGC	1 cut(s)	372
SfaNI	GCATC	8 cut(s)	115, 538, 796, 817, 843, 949, 1245, 1597
SfcI	CTRYAG	3 cut(s)	717, 732, 954
SfoI	GGCGCC	1 cut(s)	73
SfuI	TTCGAA	1 cut(s)	1489
SgfI	GCGATCGC	1 cut(s)	372
SgrAI	CRCCGGYG	1 cut(s)	374
SinI	GGWCC	3 cut(s)	1503, 1562, 1694
SmiMI	CAYNNNNRTG	4 cut(s)	801, 1544, 1724, 1740
SmlI	CTYRAG	3 cut(s)	1022, 1467, 1763
SmoI	CTYRAG	3 cut(s)	1022, 1467, 1763
Sse9I	AATT	5 cut(s)	391, 573, 689, 1322, 1368
SseBI	AGGCCT	1 cut(s)	1119
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	3 cut(s)	710, 1121, 1776
SstI	GAGCTC	2 cut(s)	273, 495
StuI	AGGCCT	1 cut(s)	1119
StyD4I	CCNGG	5 cut(s)	193, 238, 278, 1330, 1455
StyI	CCWWGG	1 cut(s)	1644
TaaI	ACNGT	5 cut(s)	85, 190, 343, 511, 1310
TaiI	ACGT	3 cut(s)	30, 54, 183
TaqI	TCGA	4 cut(s)	24, 219, 578, 1489
TasI	AATT	5 cut(s)	391, 573, 689, 1322, 1368
TauI	GCSGC	5 cut(s)	141, 156, 290, 1205, 1800
TfiI	GAWTC	3 cut(s)	451, 637, 1486
Tru1I	TTAA	2 cut(s)	1227, 1305
Tru9I	TTAA	2 cut(s)	1227, 1305
TscAI	CASTG	6 cut(s)	346, 726, 759, 931, 1153, 1600
TseFI	GTSAC	5 cut(s)	172, 331, 511, 1060, 1459
TseI	GCWGC	9 cut(s)	258, 261, 621, 716, 728, 731, 1048, 1335, 1787
Tsp45I	GTSAC	5 cut(s)	172, 331, 511, 1060, 1459
TspDTI	ATGAA	4 cut(s)	666, 1115, 1426, 1708
TspGWI	ACGGA	2 cut(s)	85, 485
TspRI	CASTG	6 cut(s)	346, 726, 759, 931, 1153, 1600
VpaK11BI	GGWCC	3 cut(s)	1503, 1562, 1694
XapI	RAATTY	1 cut(s)	391
XceI	RCATGY	1 cut(s)	800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmnI	GAANNNNTTC	2 cut(s)	666, 1106
XspI	CTAG	3 cut(s)	710, 1121, 1776
ZraI	GACGTC	1 cut(s)	181
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.