Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

17249675 .. 17252276

Gene Structure 2602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g14350.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGGACACCAAAATCGGTTCCCTCGACGTTTGCAAGCCGGCTTGCACCGACGTCGGCAGCCTCCCGAACGGCGCCACTTCGGCAATCCAAAGCACTGCCCCCTCCACCGTCAACTCCTCAGATGCCACTCTGGGTCGCCACATCGCCCGCCGACTCGTCCAAATCGGCGTTACTGACGTGTTCACTGTCCCTGGTGACTTTAACTTGACCCTCCTCGACCACCTCATCGCGGAGCCCGGGCTCACCAACATCGGCTGCTGCAACGAACTCAACGCCGGGTACGCTACTGACGGCTACGCTAGGTCGCGGGGAGTCGGGGCGTGTGTTGTTACTTTCACTGTGGGTGGGCTCAGTGTTCTCAATGCTATCGCCGGAGCTTACAGTGAGAATCTGCCATTGATTTGTATAGTTGGAGGACCCAACTCGAACGATTACGGGACGCACAGGATTCTTCACCACACTATTGGGTTACCGGATTTTAACCAAGAGCTGAGATGCTTCCAGACCGTCACTTGCTATCAGGCTGTGGTAAATAATCTGGAAGATGCTCATGAAATGATTGATACCGCAATTTCAACCGCCTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTCCTCATCCTACTTTTAGCCGGGATCCTGTTCCGTTCTCATTGTCTCCAAGATTGAGCAATCATTTGGGCTTAAAGGCTGCCGTGGAGGCGGCTGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTAGGGGGGCCTAAACTTCGAGTTGCACACGCTGGCGATGCCTTTGTTGAACTAGCTGACGCTAGTGGTTATGCTCTGGCTGTCATGCCATCTGCAAAGGGGCTTGTGCCAGAGCACCACCCCCATTTCATTGGAACATACTGGGGTGCTGTGAGCACTACCTTTTGCGCCGAGATTGTGGAGTCTGCAGATGCATACTTGTTTGATGGACCGATTTTCAATGACTACAGCTCTGTTGGATACTCTCTGCTTCTGAAGAAAGAGAAGGCAATTGTGGTGCAGCCTGATCGCGTGACCATAGAAAATGGCCCTTCATTTGGTTGTGTTCTCATGAAGGATTTCCTCCGAGCTCTGGCAAAGAGGCTCAAGCGCAACAAAACTGCTCATGAGAACTACAGCAGATCGATCTTTGTTCCCAATGGACGTCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAATGTTCTGTTCCACCACATCCAGAATACGCTGTCAAGTGAAACTGCTGTGATTGCTGATACCGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGGTTGCGGGTATGAGTTCCAAATGCAGTATGGATCAATCTATGAACTAATCCATGGTTGGTCAGTTGGAGCTACTCTTGGGTATGCTCAAGCTGTTCCTGAGAAGCGTGTGATTGCTTTCATCGGCGATGGGAGTTTCCAGGTGACTGTTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGAACATCATCTTCCTGATAAACAACGGCGGATACACAATTGGGGTGGTGATCCATGACGGACCCTACAATGTGATCAAGAACTGGAACTACACTGGACTAGTTGATGCCATCCACAACGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATTGAAGCGATTGAGACTGCAACCGGGGCGAAGAAGGATAGCTTGTGCTTCATTGAGGTGATAGCCCACAAGGATGATACCAGCAAAGAGTTGCTTCAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCAACCCTCAGTAAAGTCTCCCATCTCGTTTCCTTTCATCATCGTAGCGTCTTTCGATCTGGAAGAAGAAAAAAAATTAGTATTTGTTCCTTCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

639

Amino Acids

69.02

Weight (kDa)

7.29

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	54, 1192
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	3 cut(s)	230, 307, 1056
AciI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
AclWI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
AcsI	RAATTY	1 cut(s)	633
AcuI	CTGAAG	2 cut(s)	1040, 1763
AcyI	GRCGYC	3 cut(s)	51, 72, 1189
AfaI	GTAC	1 cut(s)	281
AfiI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
AflIII	ACRYGT	1 cut(s)	177
AgsI	TTSAA	7 cut(s)	576, 586, 742, 815, 985, 1484, 1688
AhlI	ACTAGT	1 cut(s)	1615
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	2 cut(s)	190, 1470
Alw21I	GWGCWC	3 cut(s)	882, 923, 1117
Alw26I	GTCTC	3 cut(s)	684, 1691, 1840
AlwI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
AlwNI	CAGNNNCTG	1 cut(s)	1318
Ama87I	CYCGRG	1 cut(s)	236
AoxI	GGCC	2 cut(s)	773, 1072
ApeKI	GCWGC	8 cut(s)	57, 255, 258, 618, 713, 728, 1045, 1802
ApoI	RAATTY	1 cut(s)	633
Asp700I	GAANNNNTTC	1 cut(s)	1103
AspLEI	GCGC	3 cut(s)	74, 935, 1137
AspS9I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
AsuC2I	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
AsuHPI	GGTGA	6 cut(s)	206, 235, 446, 1486, 1576, 1753
AvaI	CYCGRG	1 cut(s)	236
AvaII	GGWCC	4 cut(s)	416, 974, 1577, 1663
BamHI	GGATCC	1 cut(s)	658
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	4 cut(s)	237, 243, 351, 1117
Bbv12I	GWGCWC	3 cut(s)	882, 923, 1117
BbvI	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BccI	CCATC	5 cut(s)	862, 965, 1454, 1634, 1848
BceAI	ACGGC	4 cut(s)	85, 307, 701, 1558
BcgI	CGANNNNNNTGC	4 cut(s)	34, 68, 1034, 1068
BciT130I	CCWGG	2 cut(s)	192, 1472
BciVI	GTATCC	2 cut(s)	998, 1541
BclI	TGATCA	1 cut(s)	1590
BcnI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
BcoDI	GTCTC	3 cut(s)	684, 1691, 1840
BcuI	ACTAGT	1 cut(s)	1615
BfaI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
BfmI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BfoI	RGCGCY	1 cut(s)	75
BfuI	GTATCC	2 cut(s)	998, 1541
BglI	GCCNNNNNGGC	2 cut(s)	80, 722
Bme1390I	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
Bme18I	GGWCC	4 cut(s)	416, 974, 1577, 1663
BmeT110I	CYCGRG	1 cut(s)	236
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
BmiI	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
BmrFI	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
BmrI	ACTGGG	1 cut(s)	916
BmsI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
BmuI	ACTGGG	1 cut(s)	916
BpuEI	CTTGAG	2 cut(s)	1115, 1404
BpuMI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
Bsa29I	ATCGAT	1 cut(s)	1169
BsaHI	GRCGYC	3 cut(s)	51, 72, 1189
BsaJI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	1631, 1661
Bsc4I	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
Bse118I	RCCGGY	3 cut(s)	37, 757, 1327
Bse1I	ACTGG	3 cut(s)	911, 1604, 1615
BseBI	CCWGG	2 cut(s)	192, 1472
BseCI	ATCGAT	1 cut(s)	1169
BseDI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BseGI	GGATG	4 cut(s)	640, 1245, 1626, 1762
BseLI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
BseMII	CTCAG	5 cut(s)	132, 364, 482, 1422, 1840
BseNI	ACTGG	3 cut(s)	911, 1604, 1615
BseRI	GAGGAG	3 cut(s)	106, 203, 1691
BseXI	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BsgI	GTGCAG	2 cut(s)	1064, 1188
Bsh1236I	CGCG	3 cut(s)	230, 307, 1056
BshFI	GGCC	2 cut(s)	775, 1074
BshNI	GGYRCC	1 cut(s)	71
BshVI	ATCGAT	1 cut(s)	1169
BsiHKAI	GWGCWC	3 cut(s)	882, 923, 1117
BsiHKCI	CYCGRG	1 cut(s)	236
BslFI	GGGAC	3 cut(s)	173, 451, 1307
BslI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
BsmAI	GTCTC	3 cut(s)	684, 1691, 1840
BsmFI	GGGAC	3 cut(s)	173, 451, 1307
BsnI	GGCC	2 cut(s)	775, 1074
BsoBI	CYCGRG	1 cut(s)	236
Bsp1286I	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
Bsp143I	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Bsp19I	CCATGG	1 cut(s)	1384
BspACI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
BspANI	GGCC	2 cut(s)	775, 1074
BspCNI	CTCAG	5 cut(s)	131, 363, 483, 1423, 1839
BspDI	ATCGAT	1 cut(s)	1169
BspFNI	CGCG	3 cut(s)	230, 307, 1056
BspHI	TCATGA	3 cut(s)	550, 1095, 1150
BspLI	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
BspMAI	CTGCAG	2 cut(s)	733, 955
BspPI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
BspT107I	GGYRCC	1 cut(s)	71
BsrFI	RCCGGY	3 cut(s)	37, 757, 1327
BsrI	ACTGG	3 cut(s)	911, 1604, 1615
BssAI	RCCGGY	3 cut(s)	37, 757, 1327
BssECI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BssMI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
BssNI	GRCGYC	3 cut(s)	51, 72, 1189
BssT1I	CCWWGG	2 cut(s)	1384, 1659
Bst2UI	CCWGG	2 cut(s)	192, 1472
Bst4CI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
Bst6I	CTCTTC	1 cut(s)	1668
BstACI	GRCGYC	3 cut(s)	51, 72, 1189
BstAPI	GCANNNNNTGC	1 cut(s)	618
BstC8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1329
BstDEI	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
BstDSI	CCRYGG	2 cut(s)	717, 1384
BstEII	GGTNACC	1 cut(s)	468
BstF5I	GGATG	4 cut(s)	640, 1245, 1626, 1762
BstFNI	CGCG	3 cut(s)	230, 307, 1056
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	3 cut(s)	74, 935, 1137
BstKTI	GATC	9 cut(s)	661, 1054, 1169, 1173, 1367, 1503, 1569, 1593, 1877
BstMAI	GTCTC	3 cut(s)	684, 1691, 1840
BstMBI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
BstMWI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
BstNI	CCWGG	2 cut(s)	192, 1472
BstPI	GGTNACC	1 cut(s)	468
BstSCI	CCNGG	8 cut(s)	190, 235, 236, 275, 654, 1289, 1470, 1706
BstSFI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BstUI	CGCG	3 cut(s)	230, 307, 1056
BstV1I	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BstX2I	RGATCY	1 cut(s)	658
BstXI	CCANNNNNNTGG	2 cut(s)	464, 896
BstYI	RGATCY	1 cut(s)	658
Bsu15I	ATCGAT	1 cut(s)	1169
BsuI	GTATCC	2 cut(s)	998, 1541
BsuRI	GGCC	2 cut(s)	775, 1074
BsuTUI	ATCGAT	1 cut(s)	1169
BtgI	CCRYGG	2 cut(s)	717, 1384
BtgZI	GCGATG	4 cut(s)	127, 211, 816, 1473
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	4 cut(s)	640, 1245, 1626, 1762
BtsI	GCAGTG	2 cut(s)	93, 756
BtsIMutI	CAGTG	8 cut(s)	93, 183, 336, 358, 388, 756, 1608, 1787
Cac8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1329
CaiI	CAGNNNCTG	1 cut(s)	1318
CciI	TCATGA	3 cut(s)	550, 1095, 1150
CfoI	GCGC	3 cut(s)	74, 935, 1137
Cfr10I	RCCGGY	3 cut(s)	37, 757, 1327
Cfr13I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
Cfr9I	CCCGGG	1 cut(s)	236
ClaI	ATCGAT	1 cut(s)	1169
CseI	GACGC	3 cut(s)	448, 833, 1855
Csp6I	GTAC	1 cut(s)	280
CspCI	CAANNNNNGTGG	2 cut(s)	1542, 1577
CviAII	CATG	8 cut(s)	551, 850, 1096, 1151, 1299, 1385, 1498, 1571
CviQI	GTAC	1 cut(s)	280
DdeI	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	9 cut(s)	660, 1053, 1168, 1172, 1366, 1502, 1568, 1592, 1876
DpnII	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Eam1104I	CTCTTC	1 cut(s)	1668
EarI	CTCTTC	1 cut(s)	1668
EciI	GGCGGA	1 cut(s)	1560
Ecl136II	GAGCTC	1 cut(s)	1115
Eco130I	CCWWGG	2 cut(s)	1384, 1659
Eco24I	GRGCYC	4 cut(s)	237, 243, 351, 1117
Eco47I	GGWCC	4 cut(s)	416, 974, 1577, 1663
Eco53kI	GAGCTC	1 cut(s)	1115
Eco57I	CTGAAG	2 cut(s)	1040, 1763
Eco88I	CYCGRG	1 cut(s)	236
Eco91I	GGTNACC	1 cut(s)	468
EcoICRI	GAGCTC	1 cut(s)	1115
EcoO109I	RGGNCCY	2 cut(s)	416, 773
EcoO65I	GGTNACC	1 cut(s)	468
EcoRI	GAATTC	1 cut(s)	633
EcoRII	CCWGG	2 cut(s)	190, 1470
EcoT14I	CCWWGG	2 cut(s)	1384, 1659
EcoT22I	ATGCAT	1 cut(s)	961
EcoT38I	GRGCYC	4 cut(s)	237, 243, 351, 1117
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	2 cut(s)	1384, 1659
FaeI	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
FalI	AAGNNNNNCTT	4 cut(s)	1709, 1741, 1761, 1793
FaqI	GGGAC	3 cut(s)	173, 451, 1307
FatI	CATG	8 cut(s)	550, 849, 1095, 1150, 1298, 1384, 1497, 1570
FauI	CCCGC	3 cut(s)	155, 300, 1331
FbaI	TGATCA	1 cut(s)	1590
FokI	GGATG	4 cut(s)	627, 1232, 1613, 1769
FriOI	GRGCYC	4 cut(s)	237, 243, 351, 1117
FspBI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
GlaI	GCGC	3 cut(s)	73, 934, 1136
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	2 cut(s)	775, 1074
HgaI	GACGC	3 cut(s)	448, 833, 1855
HhaI	GCGC	3 cut(s)	74, 935, 1137
Hin1I	GRCGYC	3 cut(s)	51, 72, 1189
Hin1II	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
Hin6I	GCGC	3 cut(s)	72, 933, 1135
HinP1I	GCGC	3 cut(s)	72, 933, 1135
HincII	GTYRAC	1 cut(s)	112
HindII	GTYRAC	1 cut(s)	112
HinfI	GANTC	6 cut(s)	153, 312, 388, 448, 947, 1295
HphI	GGTGA	6 cut(s)	206, 235, 446, 1486, 1576, 1753
Hpy166II	GTNNAC	3 cut(s)	112, 183, 1494
Hpy188I	TCNGA	4 cut(s)	121, 1020, 1112, 1505
Hpy8I	GTNNAC	3 cut(s)	112, 183, 1494
Hpy99I	CGWCG	3 cut(s)	29, 53, 56
HpyAV	CCTTC	4 cut(s)	1024, 1086, 1093, 1711
HpyCH4III	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
HpyCH4IV	ACGT	4 cut(s)	27, 51, 177, 1189
HpyF10VI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
HpyF3I	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
HpySE526I	ACGT	4 cut(s)	27, 51, 177, 1189
Hsp92I	GRCGYC	3 cut(s)	51, 72, 1189
Hsp92II	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
HspAI	GCGC	3 cut(s)	72, 933, 1135
KasI	GGCGCC	1 cut(s)	71
KroI	GCCGGC	2 cut(s)	37, 1327
KroNI	GCCGGC	2 cut(s)	39, 1329
Ksp22I	TGATCA	1 cut(s)	1590
Kzo9I	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
LmnI	GCTCC	4 cut(s)	232, 374, 1400, 1678
Lsp1109I	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
LweI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
MaeI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
MaeII	ACGT	4 cut(s)	27, 51, 177, 1189
MaeIII	GTNAC	7 cut(s)	169, 194, 328, 468, 508, 1057, 1474
MalI	GATC	9 cut(s)	660, 1053, 1168, 1172, 1366, 1502, 1568, 1592, 1876
MboI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
MboII	GAAGA	9 cut(s)	443, 554, 1033, 1519, 1528, 1685, 1726, 1893, 1896
MfeI	CAATTG	2 cut(s)	1035, 1554
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
MluCI	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	4 cut(s)	147, 321, 956, 1289
MmeI	TCCRAC	3 cut(s)	391, 982, 1378
Mph1103I	ATGCAT	1 cut(s)	961
MroNI	GCCGGC	2 cut(s)	37, 1327
MroXI	GAANNNNTTC	1 cut(s)	1103
MseI	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
MslI	CAYNNNNRTG	2 cut(s)	1739, 1755
MspR9I	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
MunI	CAATTG	2 cut(s)	1035, 1554
MvaI	CCWGG	2 cut(s)	192, 1472
MvnI	CGCG	3 cut(s)	230, 307, 1056
MwoI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
NaeI	GCCGGC	2 cut(s)	39, 1329
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
NcoI	CCATGG	1 cut(s)	1384
NdeII	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
NgoMIV	GCCGGC	2 cut(s)	37, 1327
NlaIII	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
NlaIV	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
NmeAIII	GCCGAG	1 cut(s)	961
NmuCI	GTSAC	4 cut(s)	194, 508, 1057, 1474
NsiI	ATGCAT	1 cut(s)	961
PagI	TCATGA	3 cut(s)	550, 1095, 1150
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PdiI	GCCGGC	2 cut(s)	39, 1329
PdmI	GAANNNNTTC	1 cut(s)	1103
PfeI	GAWTC	2 cut(s)	388, 448
PleI	GAGTC	4 cut(s)	147, 320, 955, 1289
PluTI	GGCGCC	1 cut(s)	75
PpsI	GAGTC	4 cut(s)	147, 320, 955, 1289
PpuMI	RGGWCCY	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	1117
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	2 cut(s)	190, 1470
PspEI	GGTNACC	1 cut(s)	468
PspGI	CCWGG	2 cut(s)	190, 1470
PspN4I	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
PspPI	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
PspPPI	RGGWCCY	1 cut(s)	416
PsrI	GAACNNNNNNTAC	2 cut(s)	1589, 1621
PstI	CTGCAG	2 cut(s)	733, 955
PstNI	CAGNNNCTG	1 cut(s)	1318
PsuI	RGATCY	1 cut(s)	658
RsaI	GTAC	1 cut(s)	281
RsaNI	GTAC	1 cut(s)	280
RseI	CAYNNNNRTG	2 cut(s)	1739, 1755
SacI	GAGCTC	1 cut(s)	1117
SaqAI	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
Sau3AI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Sau96I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
SchI	GAGTC	4 cut(s)	147, 321, 956, 1289
ScrFI	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
SduI	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
SfaNI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
SfcI	CTRYAG	4 cut(s)	729, 951, 991, 1159
SfoI	GGCGCC	1 cut(s)	73
SinI	GGWCC	4 cut(s)	416, 974, 1577, 1663
SmaI	CCCGGG	1 cut(s)	238
SmiMI	CAYNNNNRTG	2 cut(s)	1739, 1755
SmlI	CTYRAG	2 cut(s)	1130, 1419
SmoI	CTYRAG	2 cut(s)	1130, 1419
SpeI	ACTAGT	1 cut(s)	1615
SrfI	GCCCGGGC	1 cut(s)	238
Sse9I	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
SsiI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
SstI	GAGCTC	1 cut(s)	1117
StyD4I	CCNGG	8 cut(s)	190, 235, 236, 275, 654, 1289, 1470, 1706
StyI	CCWWGG	2 cut(s)	1384, 1659
TaaI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
TaiI	ACGT	4 cut(s)	30, 54, 180, 1192
TaqI	TCGA	6 cut(s)	24, 216, 425, 784, 1169, 1873
TaqII	GACCGA	1 cut(s)	991
TasI	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
TauI	GCSGC	2 cut(s)	728, 1815
TfiI	GAWTC	2 cut(s)	388, 448
Tru1I	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
Tru9I	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
TscAI	CASTG	8 cut(s)	100, 190, 343, 358, 388, 756, 1615, 1787
TseFI	GTSAC	4 cut(s)	194, 508, 1057, 1474
TseI	GCWGC	8 cut(s)	57, 255, 258, 618, 713, 728, 1045, 1802
Tsp45I	GTSAC	4 cut(s)	194, 508, 1057, 1474
TspDTI	ATGAA	8 cut(s)	567, 883, 1068, 1112, 1389, 1441, 1723, 1844
TspGWI	ACGGA	3 cut(s)	657, 1590, 1655
TspMI	CCCGGG	1 cut(s)	236
TspRI	CASTG	8 cut(s)	100, 190, 343, 358, 388, 756, 1615, 1787
VpaK11BI	GGWCC	4 cut(s)	416, 974, 1577, 1663
XapI	RAATTY	1 cut(s)	633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmaI	CCCGGG	1 cut(s)	236
XmnI	GAANNNNTTC	1 cut(s)	1103
XspI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
ZraI	GACGTC	2 cut(s)	52, 1190
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.