Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

9884264 .. 9888464

Gene Structure 4201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG115300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1533 bp

ATGGACACCAAGATTGGCTCCCTCGACGTTTGCAAGGCCGACAACCACGACGTCGGTTGTTTACCAAACAGCGCCGCTTGCGCCGTCCAAAACTCGGTCCCTTCCACCACGTTCAACTCCGCCGACGCCACTCTCGGCCGCCACCTGGCACGCCGCCTCGTCCAAATCGGCGTCACCGACGTCTTCACCGTCCCCGGCGACTTCAACTTGACCCTTCTCGACCACCTCATCGCCGAGCCCGGGCTCACCAACATTGGCTGCTGCAATGAGCTCAACGCCGGATACGCAGCCGACGGCTACGCGCGGTCGCGTGGCGTCGGGGCGTGTGCGGTGACTTTCACCGTCGGTGGACTGAGTGTGTTGAACGCGATCGCTGGCGCGTACAGTGAGAACTTGCCGGTGATTTGTATTGTTGGAGGGCCCAACTCCAATGATTACGGAACTAACAGGATTCTTCACCATACTATTGGGGTGCCGGACTTTAGCCAAGAGCTCCGGTGCTTTCAGACTGTGACTTGCTTTCAGGCTGTGGTGAATAATCTGGAGGATGCACATGAGTTGATTGATACGGCAATTTCGACTGCGTTGAAAGAAAGCAAGCCTGTGTATATCAGCATAGGCTGCAACTTGGCTGGGATTCCTCATCCTACTTTTAGCCGTGACCCTGTTCCATTTTCATTGTCTCCAAAATTGAGCAATAAGATGGGATTAGAGGCTGCAGTGGAGGCAGCGGCAGAGTTCTTGAACAAGGCAGTTAAGCCGGTAATGGTGGGCGGGCCCAAACTGCGTGCTGCACATGCTGGTGATGCCTTTGTTGAGCTGGCTGATGCTTCTGGTTTTGCTCTGGCTGTTATGCCATCAGCAAAGGGGCAAGTGCCAGAGCACCATCCCCATTTCATAGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGTGCTGAGATTGTGGAGTCCGCAGATGCATACTTGTTTGCTGGGCCAATTTTCAATGACTACAGCTCCGTTGGGTACTCGCTCCTTCTCAAGAAAGAGAAGGCGATCATTGTGCAGCCTGATCGTGTGACGATAGGAAATGGCCCTGCATTTGGTTGTGTTCTCATGAAGGATTTCCTGTTATGCCTAGCAAAGAAGCTGAAGCATAACAATACTGCTCATGAGAACTACCGCAGGATCTTTGTGCCCGATGGCCACCCTCTAAAGGCTGCACCCAGAGAACCTTTGAGGGTTAATGTTCTGTTCCAGCACATTCAGAAGATGCTGTCAGCTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTCAACTGCCAGAAGCTGAAATTGCCACCTGCCTGCGGGTATGAATTCCAAATGCAATATGGATCAATAGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAGGCTGTACCTGAGAAGCGAGTGCTTTCTTTCATTGGTGATGGGAGCTTCCAGGTATGCAACTTTGATCACATTACTAGTCTTGTGAGTTCTGCAATAGCCAAAGTGATTATAGAGATTGTATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

510

Amino Acids

54.38

Weight (kDa)

6.05

Isoelectric Point (pI)

29.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	44 - 149	7.7e-30	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	241 - 359	1.2e-22	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	451 - 501	3.9e-06	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1339
AatII	GACGTC	2 cut(s)	54, 183
Acc36I	ACCTGC	1 cut(s)	1339
AccB1I	GGYRCC	1 cut(s)	472
AccII	CGCG	5 cut(s)	302, 304, 310, 368, 380
AclWI	GGATC	2 cut(s)	1178, 1372
AcoI	YGGCCR	2 cut(s)	136, 1186
AcsI	RAATTY	1 cut(s)	1346
AcuI	CTGAAG	1 cut(s)	1154
AcyI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
AfaI	GTAC	3 cut(s)	383, 1010, 1413
AfiI	CCNNNNNNNGG	6 cut(s)	94, 145, 240, 1085, 1198, 1337
AgsI	TTSAA	7 cut(s)	115, 205, 364, 589, 745, 988, 1306
AhlI	ACTAGT	1 cut(s)	1481
AjnI	CCWGG	2 cut(s)	144, 1455
AloI	GAACNNNNNNTCC	2 cut(s)	1286, 1318
AluBI	AGCT	8 cut(s)	271, 493, 820, 999, 1132, 1265, 1318, 1452
AluI	AGCT	8 cut(s)	271, 493, 820, 999, 1132, 1265, 1318, 1452
Alw21I	GWGCWC	4 cut(s)	273, 495, 885, 926
Alw26I	GTCTC	2 cut(s)	687, 1280
AlwI	GGATC	2 cut(s)	1178, 1372
AlwNI	CAGNNNCTG	1 cut(s)	1318
Ama87I	CYCGRG	1 cut(s)	239
AoxI	GGCC	7 cut(s)	36, 136, 419, 776, 977, 1075, 1186
ApaI	GGGCCC	2 cut(s)	423, 780
ApeKI	GCWGC	9 cut(s)	258, 261, 287, 621, 716, 728, 791, 1048, 1202
ApoI	RAATTY	1 cut(s)	1346
AsiSI	GCGATCGC	1 cut(s)	372
Asp700I	GAANNNNTTC	1 cut(s)	1106
AspLEI	GCGC	4 cut(s)	74, 83, 304, 380
AspS9I	GGNCC	7 cut(s)	97, 419, 420, 776, 777, 977, 1076
AsuC2I	CCSGG	3 cut(s)	195, 240, 241
AvaI	CYCGRG	1 cut(s)	239
AvaII	GGWCC	1 cut(s)	97
BaeGI	GKGCMC	3 cut(s)	423, 780, 1182
BalI	TGGCCA	1 cut(s)	1188
BanI	GGYRCC	1 cut(s)	472
BanII	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
BbsI	GAAGAC	1 cut(s)	175
Bbv12I	GWGCWC	4 cut(s)	273, 495, 885, 926
BbvI	GCAGC	9 cut(s)	245, 248, 299, 608, 703, 740, 778, 1060, 1189
BccI	CCATC	5 cut(s)	697, 865, 894, 1178, 1439
BceAI	ACGGC	4 cut(s)	68, 310, 585, 642
BcgI	CGANNNNNNTGC	4 cut(s)	1027, 1037, 1061, 1071
BciT130I	CCWGG	2 cut(s)	146, 1457
BciVI	GTATCC	1 cut(s)	275
BclI	TGATCA	1 cut(s)	1471
BcnI	CCSGG	3 cut(s)	195, 240, 241
BcoDI	GTCTC	2 cut(s)	687, 1280
BcuI	ACTAGT	1 cut(s)	1481
BfaI	CTAG	2 cut(s)	1121, 1482
BfmI	CTRYAG	2 cut(s)	717, 994
BfoI	RGCGCY	1 cut(s)	75
BfuAI	ACCTGC	1 cut(s)	1339
BfuI	GTATCC	1 cut(s)	275
Bme1390I	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
Bme18I	GGWCC	1 cut(s)	97
BmeT110I	CYCGRG	1 cut(s)	239
BmgT120I	GGNCC	7 cut(s)	97, 419, 420, 776, 777, 977, 1076
BmiI	GGNNCC	5 cut(s)	19, 99, 421, 474, 778
BmrFI	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
BmrI	ACTGGG	1 cut(s)	919
BmsI	GCATC	5 cut(s)	538, 796, 817, 949, 1245
BmuI	ACTGGG	1 cut(s)	919
BpiI	GAAGAC	1 cut(s)	175
BpmI	CTGGAG	1 cut(s)	563
Bpu10I	CCTNAGC	1 cut(s)	1404
BpuEI	CTTGAG	1 cut(s)	1007
BpuMI	CCSGG	3 cut(s)	195, 240, 241
BsaHI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
BsaJI	CCNNGG	2 cut(s)	193, 239
BsaWI	WCCGGW	1 cut(s)	495
BsaXI	ACNNNNNCTCC	2 cut(s)	983, 1013
Bsc4I	CCNNNNNNNGG	6 cut(s)	94, 145, 240, 1085, 1198, 1337
Bse118I	RCCGGY	2 cut(s)	397, 760
Bse1I	ACTGG	1 cut(s)	914
Bse3DI	GCAATG	1 cut(s)	271
BseBI	CCWGG	2 cut(s)	146, 1457
BseDI	CCNNGG	2 cut(s)	193, 239
BseGI	GGATG	3 cut(s)	553, 643, 886
BseLI	CCNNNNNNNGG	6 cut(s)	94, 145, 240, 1085, 1198, 1337
BseMI	GCAATG	1 cut(s)	271
BseMII	CTCAG	5 cut(s)	344, 930, 1275, 1407, 1418
BseNI	ACTGG	1 cut(s)	914
BseSI	GKGCMC	3 cut(s)	423, 780, 1182
BseX3I	CGGCCG	1 cut(s)	136
BseXI	GCAGC	9 cut(s)	245, 248, 299, 608, 703, 740, 778, 1060, 1189
BseYI	CCCAGC	2 cut(s)	632, 974
BsgI	GTGCAG	3 cut(s)	777, 1067, 1188
Bsh1236I	CGCG	5 cut(s)	302, 304, 310, 368, 380
Bsh1285I	CGRYCG	3 cut(s)	139, 308, 372
BshFI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BshNI	GGYRCC	1 cut(s)	472
BsiEI	CGRYCG	3 cut(s)	139, 308, 372
BsiHKAI	GWGCWC	4 cut(s)	273, 495, 885, 926
BsiHKCI	CYCGRG	1 cut(s)	239
BsiSI	CCGG	7 cut(s)	195, 240, 279, 398, 476, 496, 761
BslFI	GGGAC	3 cut(s)	83, 176, 1307
BslI	CCNNNNNNNGG	6 cut(s)	94, 145, 240, 1085, 1198, 1337
BsmAI	GTCTC	2 cut(s)	687, 1280
BsmFI	GGGAC	3 cut(s)	83, 176, 1307
BsnI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BsoBI	CYCGRG	1 cut(s)	239
Bsp120I	GGGCCC	2 cut(s)	419, 776
Bsp1286I	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
Bsp143I	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
BspANI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BspCNI	CTCAG	5 cut(s)	345, 931, 1276, 1408, 1417
BspFNI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BspHI	TCATGA	2 cut(s)	1098, 1153
BspLI	GGNNCC	5 cut(s)	19, 99, 421, 474, 778
BspMAI	CTGCAG	1 cut(s)	721
BspMI	ACCTGC	1 cut(s)	1339
BspPI	GGATC	2 cut(s)	1178, 1372
BspT107I	GGYRCC	1 cut(s)	472
BsrDI	GCAATG	1 cut(s)	271
BsrFI	RCCGGY	2 cut(s)	397, 760
BsrI	ACTGG	1 cut(s)	914
BssAI	RCCGGY	2 cut(s)	397, 760
BssECI	CCNNGG	2 cut(s)	193, 239
BssMI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
BssNI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
Bst2UI	CCWGG	2 cut(s)	146, 1457
Bst4CI	ACNGT	4 cut(s)	190, 343, 386, 511
BstACI	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
BstAPI	GCANNNNNTGC	1 cut(s)	621
BstC8I	GCNNGC	8 cut(s)	79, 151, 376, 599, 776, 789, 822, 1336
BstDEI	CTNAG	5 cut(s)	353, 939, 1284, 1404, 1416
BstF5I	GGATG	3 cut(s)	553, 643, 886
BstFNI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	4 cut(s)	74, 83, 304, 380
BstKTI	GATC	6 cut(s)	372, 1041, 1057, 1173, 1367, 1474
BstMAI	GTCTC	2 cut(s)	687, 1280
BstMBI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
BstMCI	CGRYCG	3 cut(s)	139, 308, 372
BstNI	CCWGG	2 cut(s)	146, 1457
BstNSI	RCATGY	1 cut(s)	800
BstSCI	CCNGG	5 cut(s)	144, 193, 238, 239, 1455
BstSFI	CTRYAG	2 cut(s)	717, 994
BstSLI	GKGCMC	3 cut(s)	423, 780, 1182
BstUI	CGCG	5 cut(s)	302, 304, 310, 368, 380
BstV1I	GCAGC	9 cut(s)	245, 248, 299, 608, 703, 740, 778, 1060, 1189
BstV2I	GAAGAC	1 cut(s)	175
BstX2I	RGATCY	1 cut(s)	1170
BstXI	CCANNNNNNTGG	1 cut(s)	467
BstYI	RGATCY	1 cut(s)	1170
BstZI	CGGCCG	1 cut(s)	136
BsuI	GTATCC	1 cut(s)	275
BsuRI	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
BtgZI	GCGATG	1 cut(s)	214
BtsCI	GGATG	3 cut(s)	553, 643, 886
BtsI	GCAGTG	2 cut(s)	726, 924
BtsIMutI	CAGTG	3 cut(s)	391, 726, 924
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	8 cut(s)	79, 151, 376, 599, 776, 789, 822, 1336
CaiI	CAGNNNCTG	1 cut(s)	1318
CciI	TCATGA	2 cut(s)	1098, 1153
CfoI	GCGC	4 cut(s)	74, 83, 304, 380
Cfr10I	RCCGGY	2 cut(s)	397, 760
Cfr13I	GGNCC	7 cut(s)	97, 419, 420, 776, 777, 977, 1076
Cfr9I	CCCGGG	1 cut(s)	239
CseI	GACGC	3 cut(s)	134, 160, 304
Csp6I	GTAC	3 cut(s)	382, 1009, 1412
CviAII	CATG	5 cut(s)	554, 797, 1099, 1154, 1299
CviQI	GTAC	3 cut(s)	382, 1009, 1412
DdeI	CTNAG	5 cut(s)	353, 939, 1284, 1404, 1416
DpnI	GATC	6 cut(s)	371, 1040, 1056, 1172, 1366, 1473
DpnII	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
EaeI	YGGCCR	2 cut(s)	136, 1186
EagI	CGGCCG	1 cut(s)	136
EciI	GGCGGA	1 cut(s)	109
Ecl136II	GAGCTC	2 cut(s)	271, 493
EclXI	CGGCCG	1 cut(s)	136
Eco24I	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
Eco47I	GGWCC	1 cut(s)	97
Eco52I	CGGCCG	1 cut(s)	136
Eco53kI	GAGCTC	2 cut(s)	271, 493
Eco57I	CTGAAG	1 cut(s)	1154
Eco88I	CYCGRG	1 cut(s)	239
EcoICRI	GAGCTC	2 cut(s)	271, 493
EcoO109I	RGGNCCY	1 cut(s)	419
EcoRI	GAATTC	1 cut(s)	1346
EcoRII	CCWGG	2 cut(s)	144, 1455
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
FaeI	CATG	5 cut(s)	557, 800, 1102, 1157, 1302
FaqI	GGGAC	3 cut(s)	83, 176, 1307
FatI	CATG	5 cut(s)	553, 796, 1098, 1153, 1298
FauI	CCCGC	2 cut(s)	767, 1331
FbaI	TGATCA	1 cut(s)	1471
FokI	GGATG	3 cut(s)	560, 630, 873
FriOI	GRGCYC	6 cut(s)	240, 246, 273, 423, 495, 780
FspBI	CTAG	2 cut(s)	1121, 1482
GlaI	GCGC	4 cut(s)	73, 82, 303, 379
GsaI	CCCAGC	2 cut(s)	636, 978
GsuI	CTGGAG	1 cut(s)	563
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	7 cut(s)	38, 138, 421, 778, 979, 1077, 1188
HapII	CCGG	7 cut(s)	195, 240, 279, 398, 476, 496, 761
HgaI	GACGC	3 cut(s)	134, 160, 304
HhaI	GCGC	4 cut(s)	74, 83, 304, 380
Hin1I	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
Hin1II	CATG	5 cut(s)	557, 800, 1102, 1157, 1302
Hin6I	GCGC	4 cut(s)	72, 81, 302, 378
HinP1I	GCGC	4 cut(s)	72, 81, 302, 378
HinfI	GANTC	4 cut(s)	451, 637, 950, 1295
HpaII	CCGG	7 cut(s)	195, 240, 279, 398, 476, 496, 761
Hpy166II	GTNNAC	2 cut(s)	62, 350
Hpy188I	TCNGA	2 cut(s)	507, 1251
Hpy188III	TCNNGA	6 cut(s)	218, 542, 742, 1024, 1099, 1154
Hpy8I	GTNNAC	2 cut(s)	62, 350
Hpy99I	CGWCG	8 cut(s)	29, 53, 56, 128, 182, 296, 320, 347
HpyAV	CCTTC	5 cut(s)	111, 224, 1027, 1028, 1096
HpyCH4III	ACNGT	4 cut(s)	190, 343, 386, 511
HpyCH4IV	ACGT	4 cut(s)	27, 51, 110, 180
HpyF3I	CTNAG	5 cut(s)	353, 939, 1284, 1404, 1416
HpySE526I	ACGT	4 cut(s)	27, 51, 110, 180
Hsp92I	GRCGYC	5 cut(s)	51, 126, 171, 180, 315
Hsp92II	CATG	5 cut(s)	557, 800, 1102, 1157, 1302
HspAI	GCGC	4 cut(s)	72, 81, 302, 378
Ksp22I	TGATCA	1 cut(s)	1471
Kzo9I	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
LmnI	GCTCC	6 cut(s)	23, 498, 1004, 1020, 1385, 1449
Lsp1109I	GCAGC	9 cut(s)	245, 248, 299, 608, 703, 740, 778, 1060, 1189
LweI	GCATC	5 cut(s)	538, 796, 817, 949, 1245
MaeI	CTAG	2 cut(s)	1121, 1482
MaeII	ACGT	4 cut(s)	27, 51, 110, 180
MaeIII	GTNAC	5 cut(s)	172, 331, 511, 659, 1060
MalI	GATC	6 cut(s)	371, 1040, 1056, 1172, 1366, 1473
MboI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
MboII	GAAGA	3 cut(s)	175, 446, 1264
MflI	RGATCY	1 cut(s)	1170
MhlI	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
MlsI	TGGCCA	1 cut(s)	1188
MluCI	AATT	5 cut(s)	573, 689, 981, 1322, 1346
MluNI	TGGCCA	1 cut(s)	1188
MlyI	GAGTC	2 cut(s)	959, 1289
MmeI	TCCRAC	2 cut(s)	394, 1363
Mox20I	TGGCCA	1 cut(s)	1188
Mph1103I	ATGCAT	1 cut(s)	964
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	1 cut(s)	1188
MseI	TTAA	2 cut(s)	756, 1227
MslI	CAYNNNNRTG	1 cut(s)	801
Msp20I	TGGCCA	1 cut(s)	1188
MspA1I	CMGCKG	2 cut(s)	731, 1265
MspI	CCGG	7 cut(s)	195, 240, 279, 398, 476, 496, 761
MspR9I	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
MvaI	CCWGG	2 cut(s)	146, 1457
MvnI	CGCG	5 cut(s)	302, 304, 310, 368, 380
NciI	CCSGG	3 cut(s)	195, 240, 241
NdeII	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
NlaIII	CATG	5 cut(s)	557, 800, 1102, 1157, 1302
NlaIV	GGNNCC	5 cut(s)	19, 99, 421, 474, 778
NmeAIII	GCCGAG	2 cut(s)	114, 259
NmuCI	GTSAC	5 cut(s)	172, 331, 511, 659, 1060
NsiI	ATGCAT	1 cut(s)	964
NspI	RCATGY	1 cut(s)	800
PagI	TCATGA	2 cut(s)	1098, 1153
PaqCI	CACCTGC	1 cut(s)	1339
PcsI	WCGNNNNNNNCGW	3 cut(s)	174, 186, 575
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	2 cut(s)	451, 637
Ple19I	CGATCG	1 cut(s)	372
PleI	GAGTC	2 cut(s)	958, 1289
PpsI	GAGTC	2 cut(s)	958, 1289
Psp124BI	GAGCTC	2 cut(s)	273, 495
Psp6I	CCWGG	2 cut(s)	144, 1455
PspFI	CCCAGC	2 cut(s)	632, 974
PspGI	CCWGG	2 cut(s)	144, 1455
PspN4I	GGNNCC	5 cut(s)	19, 99, 421, 474, 778
PspOMI	GGGCCC	2 cut(s)	419, 776
PspPI	GGNCC	7 cut(s)	97, 419, 420, 776, 777, 977, 1076
PstI	CTGCAG	1 cut(s)	721
PstNI	CAGNNNCTG	1 cut(s)	1318
PsuI	RGATCY	1 cut(s)	1170
PvuI	CGATCG	1 cut(s)	372
PvuII	CAGCTG	1 cut(s)	1265
RgaI	GCGATCGC	1 cut(s)	372
RsaI	GTAC	3 cut(s)	383, 1010, 1413
RsaNI	GTAC	3 cut(s)	382, 1009, 1412
RseI	CAYNNNNRTG	1 cut(s)	801
SacI	GAGCTC	2 cut(s)	273, 495
SaqAI	TTAA	2 cut(s)	756, 1227
Sau3AI	GATC	6 cut(s)	369, 1038, 1054, 1170, 1364, 1471
Sau96I	GGNCC	7 cut(s)	97, 419, 420, 776, 777, 977, 1076
SchI	GAGTC	2 cut(s)	959, 1289
ScrFI	CCNGG	5 cut(s)	146, 195, 240, 241, 1457
SduI	GDGCHC	9 cut(s)	240, 246, 273, 423, 495, 780, 885, 926, 1182
SfaAI	GCGATCGC	1 cut(s)	372
SfaNI	GCATC	5 cut(s)	538, 796, 817, 949, 1245
SfcI	CTRYAG	2 cut(s)	717, 994
SgfI	GCGATCGC	1 cut(s)	372
SinI	GGWCC	1 cut(s)	97
SmaI	CCCGGG	1 cut(s)	241
SmiMI	CAYNNNNRTG	1 cut(s)	801
SmlI	CTYRAG	1 cut(s)	1022
SmoI	CTYRAG	1 cut(s)	1022
SpeI	ACTAGT	1 cut(s)	1481
SrfI	GCCCGGGC	1 cut(s)	241
Sse9I	AATT	5 cut(s)	573, 689, 981, 1322, 1346
SspMI	CTAG	2 cut(s)	1121, 1482
SstI	GAGCTC	2 cut(s)	273, 495
StyD4I	CCNGG	5 cut(s)	144, 193, 238, 239, 1455
TaaI	ACNGT	4 cut(s)	190, 343, 386, 511
TaiI	ACGT	4 cut(s)	30, 54, 113, 183
TaqI	TCGA	3 cut(s)	24, 219, 578
TaqII	GACCGA	1 cut(s)	85
TasI	AATT	5 cut(s)	573, 689, 981, 1322, 1346
TauI	GCSGC	4 cut(s)	77, 141, 156, 734
TfiI	GAWTC	2 cut(s)	451, 637
Tru1I	TTAA	2 cut(s)	756, 1227
Tru9I	TTAA	2 cut(s)	756, 1227
TscAI	CASTG	3 cut(s)	391, 726, 931
TseFI	GTSAC	5 cut(s)	172, 331, 511, 659, 1060
TseI	GCWGC	9 cut(s)	258, 261, 287, 621, 716, 728, 791, 1048, 1202
Tsp45I	GTSAC	5 cut(s)	172, 331, 511, 659, 1060
TspDTI	ATGAA	5 cut(s)	666, 886, 1115, 1359, 1426
TspGWI	ACGGA	2 cut(s)	453, 991
TspMI	CCCGGG	1 cut(s)	239
TspRI	CASTG	3 cut(s)	391, 726, 931
VpaK11BI	GGWCC	1 cut(s)	97
XapI	RAATTY	1 cut(s)	1346
XceI	RCATGY	1 cut(s)	800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmaI	CCCGGG	1 cut(s)	239
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	2 cut(s)	1121, 1482
ZraI	GACGTC	2 cut(s)	52, 181
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.