Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

132264 .. 134920

Gene Structure 2657 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G01330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGGACGTCCGAAGTCTACCAAGCAACGGCGTCGCAACAATCCAAGACTCCGCTCCCACCGCCGCAACCATACTCGGCTCCTCTGCAGCGACTCTTGGACGTCACTTATCCCGCCGTCTTGTCCAAGCCGGAGTCACCGACATTTTCACTGTTCCCGGTGATTTCAACCTATCTCTGCTTGACCAGCTCATCGCCAACCCGGAGCTCAACAACATCGGGTGCTGTAACGAGCTAAACGCCGGTTACGCCGCCGATGGATACGCTCGATCTCGTGGAGTCGGTGCTTGCGTCGTGACTTTCACCGTTGGTGGACTCAGCGTTTTGAACGCTATCGCTGGTGCGTACAGTGAGAACCTACCAGTTATATGTATCGTTGGAGGTCCTAACTCCAACGATTTTGGCACTAACCGGATTCTTCATCACACCATTGGTTTACCTGATTTTAGCCAAGAGCTTAGGTGTTTCCAAACTGTTACTTGCTATCAGGCTGTGGTGAACCATTTAGAGGATGCTCATGAACAGATCGACAAGGCGATAGCAACAGCTCTGAGAGAGAGCAAGCCTGTGTACATTAGCATCAGCTGTAACTTAGCTGCCATTCCTCATCCTACATTTGCTAGTTATCCTGTCCCTTTTGACCTAACTCCAAGGTTGAGCAACAAGGATTGTTTAGAAGCTGCCGTGGAAGCAACATTGGAGTTTCTAAACAAGGCCGTGAAGCCCGTTATGGTCGGTGGTCCCAAGTTGCGTGTGGCTAAAGCTAGGGATGCCTTTGTGGAGCTAGCTGATGCTTCAGGCTACCCCGTGGCAGTGATGCCATCTGCCAAAGGCTTTGTCCCAGAGAACCATCCTCATTTCATTGGCACTTACTGGGGAGCAGTGAGCACTCTTTTCTGCTCTGAGATTGTCGAGTCCGCGGATGCTTACATCTTCGCAGGTCCAATCTTCAACGACTACAGCTCCGTTGGTTACTCACTTCTCCTCAAGAAAGAAAAAGCCATCATTGTGCATCCTGATAGTGTCGTTGTGGCTAATGGTCCTACCTTTGGTTGCGTTCGGATGAGCGAATTCTTCAGGGAGTTGGCTAAGAGGGTGAAACCTAACAAGACGGCTTATGAGAACTACCATAGAATCTTTGTCCCTGAAGGTAAGCCTTTGAAATGCAAACCAAGAGAGCCTTTGAGAATCAACGCAATGTTCCAACACATTCAGAAGATGCTCTCTAATGAAACCGCTGTGATTGCTGAAACTGGTGATTCTTGGTTCAATTGTCAGAAACTTAAGCTGCCTAAAGGATGTGGGTACGAGTTTCAAATGCAGTATGGATCAATTGGTTGGTCTGTTGGTGCGACTCTAGGATACGCTCAAGCAACACCGGAGAAGAGAGTGTTGTCTTTCATTGGAGACGGAAGTTTCCAAGTGACGGCTCAGGACGTTTCCACAATGATTCGTAACGGACAGAAGACCATCATCTTCCTGATCAACAACGGTGGCTACACCATTGAAGTTGAGATTCATGATGGTCCTTACAATGTGATCAAGAATTGGAACTACACTGGTCTTGTCGATGCTATTCATAATGGTGAAGGCAAATGCTGGACCACAAAGGTGAGATACGAGGAGGAGTTAGTGGAAGCGATTAACACGGCGACATTGGAGAAGAAAGATAGTCTTTGTTTCATTGAAGTGATTGTTCACAAGGATGATACGAGCAAAGAGTTGCTTGAGTGGGGCTCTCGCGTCTCAGCTGCTAATGGTCGTCCTCCCAATCCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

592

Amino Acids

64.48

Weight (kDa)

6.09

Isoelectric Point (pI)

31.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	31 - 135	7e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	228 - 342	3.2e-23	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	438 - 563	5.3e-15	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	9, 103
Acc36I	ACCTGC	1 cut(s)	926
AccBSI	CCGCTC	1 cut(s)	53
AccI	GTMKAC	1 cut(s)	16
AccII	CGCG	2 cut(s)	917, 1740
AciI	CCGC	8 cut(s)	51, 60, 63, 112, 249, 915, 917, 1233
AclWI	GGATC	1 cut(s)	1333
AcsI	RAATTY	1 cut(s)	1067
AcuI	CTGAAG	3 cut(s)	777, 1057, 1164
AcyI	GRCGYC	3 cut(s)	6, 30, 100
AfaI	GTAC	3 cut(s)	344, 569, 1304
AfiI	CCNNNNNNNGG	4 cut(s)	26, 505, 1046, 1423
AflII	CTTAAG	1 cut(s)	1280
AgsI	TTSAA	8 cut(s)	166, 325, 949, 1159, 1267, 1313, 1505, 1685
AleI	CACNNNNGTG	1 cut(s)	1607
Alw21I	GWGCWC	2 cut(s)	207, 887
Alw26I	GTCTC	2 cut(s)	1398, 1747
AlwI	GGATC	1 cut(s)	1333
AoxI	GGCC	1 cut(s)	711
ApeKI	GCWGC	5 cut(s)	86, 593, 677, 1285, 1748
ApoI	RAATTY	1 cut(s)	1067
ArsI	GACNNNNNNTTYG	2 cut(s)	819, 851
AspS9I	GGNCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
AsuC2I	CCSGG	2 cut(s)	156, 200
AsuHPI	GGTGA	8 cut(s)	127, 170, 292, 505, 1105, 1265, 1595, 1621
AsuNHI	GCTAGC	1 cut(s)	781
AvaII	GGWCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
BanII	GRGCYC	2 cut(s)	207, 1736
BauI	CACGAG	1 cut(s)	270
BbsI	GAAGAC	1 cut(s)	1469
Bbv12I	GWGCWC	2 cut(s)	207, 887
BbvI	GCAGC	5 cut(s)	98, 580, 664, 1272, 1735
BccI	CCATC	6 cut(s)	248, 826, 855, 1007, 1475, 1514
BceAI	ACGGC	7 cut(s)	43, 99, 665, 698, 1125, 1440, 1662
BcgI	CGANNNNNNTGC	2 cut(s)	13, 47
BciVI	GTATCC	2 cut(s)	251, 1352
BclI	TGATCA	2 cut(s)	1479, 1536
BcnI	CCSGG	2 cut(s)	156, 200
BcoDI	GTCTC	2 cut(s)	1398, 1747
BfaI	CTAG	4 cut(s)	618, 762, 782, 1355
BfmI	CTRYAG	2 cut(s)	84, 955
BfrI	CTTAAG	1 cut(s)	1280
BfuAI	ACCTGC	1 cut(s)	926
BfuI	GTATCC	2 cut(s)	251, 1352
BisI	GCNGC	7 cut(s)	63, 87, 249, 594, 678, 1286, 1749
BlsI	GCNGC	7 cut(s)	64, 88, 250, 595, 679, 1287, 1750
Bme1390I	CCNGG	2 cut(s)	156, 200
Bme18I	GGWCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
BmgT120I	GGNCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
BmiI	GGNNCC	2 cut(s)	79, 739
BmrFI	CCNGG	2 cut(s)	156, 200
BmrI	ACTGGG	1 cut(s)	880
BmsI	GCATC	9 cut(s)	499, 585, 757, 778, 804, 910, 1018, 1206, 1558
BmtI	GCTAGC	1 cut(s)	785
BmuI	ACTGGG	1 cut(s)	880
BpiI	GAAGAC	1 cut(s)	1469
BplI	GAGNNNNNCTC	2 cut(s)	1718, 1750
Bpu10I	CCTNAGC	2 cut(s)	455, 1428
BpuEI	CTTGAG	3 cut(s)	968, 1350, 1745
BpuMI	CCSGG	2 cut(s)	156, 200
BsaHI	GRCGYC	3 cut(s)	6, 30, 100
BsaJI	CCNNGG	4 cut(s)	647, 681, 804, 915
BsaWI	WCCGGW	2 cut(s)	408, 1375
BsaXI	ACNNNNNCTCC	2 cut(s)	944, 974
Bsc4I	CCNNNNNNNGG	4 cut(s)	26, 505, 1046, 1423
Bse118I	RCCGGY	1 cut(s)	239
Bse1I	ACTGG	4 cut(s)	359, 875, 1255, 1561
Bse3DI	GCAATG	1 cut(s)	1200
BseDI	CCNNGG	4 cut(s)	647, 681, 804, 915
BseGI	GGATG	9 cut(s)	514, 604, 772, 847, 925, 1009, 1065, 1301, 1708
BseLI	CCNNNNNNNGG	4 cut(s)	26, 505, 1046, 1423
BseMI	GCAATG	1 cut(s)	1200
BseMII	CTCAG	5 cut(s)	328, 539, 891, 1442, 1758
BseNI	ACTGG	4 cut(s)	359, 875, 1255, 1561
BseRI	GAGGAG	4 cut(s)	70, 971, 1634, 1637
BseXI	GCAGC	5 cut(s)	98, 580, 664, 1272, 1735
Bsh1236I	CGCG	2 cut(s)	917, 1740
BshFI	GGCC	1 cut(s)	713
BsiHKAI	GWGCWC	2 cut(s)	207, 887
BsiSI	CCGG	6 cut(s)	129, 156, 200, 240, 409, 1376
BslFI	GGGAC	4 cut(s)	614, 723, 821, 1124
BslI	CCNNNNNNNGG	4 cut(s)	26, 505, 1046, 1423
BsmAI	GTCTC	2 cut(s)	1398, 1747
BsmBI	CGTCTC	2 cut(s)	1398, 1747
BsmFI	GGGAC	4 cut(s)	614, 723, 821, 1124
BsnI	GGCC	1 cut(s)	713
Bsp1286I	GDGCHC	3 cut(s)	207, 887, 1736
Bsp1407I	TGTACA	1 cut(s)	567
Bsp143I	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
BspACI	CCGC	8 cut(s)	51, 60, 63, 112, 249, 915, 917, 1233
BspANI	GGCC	1 cut(s)	713
BspCNI	CTCAG	5 cut(s)	327, 540, 892, 1441, 1757
BspFNI	CGCG	2 cut(s)	917, 1740
BspHI	TCATGA	2 cut(s)	514, 1516
BspLI	GGNNCC	2 cut(s)	79, 739
BspMAI	CTGCAG	1 cut(s)	88
BspMI	ACCTGC	1 cut(s)	926
BspOI	GCTAGC	1 cut(s)	785
BspPI	GGATC	1 cut(s)	1333
BspTI	CTTAAG	1 cut(s)	1280
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	1 cut(s)	1200
BsrFI	RCCGGY	1 cut(s)	239
BsrGI	TGTACA	1 cut(s)	567
BsrI	ACTGG	4 cut(s)	359, 875, 1255, 1561
BssAI	RCCGGY	1 cut(s)	239
BssECI	CCNNGG	4 cut(s)	647, 681, 804, 915
BssMI	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
BssNI	GRCGYC	3 cut(s)	6, 30, 100
BssSI	CACGAG	1 cut(s)	270
BssT1I	CCWWGG	1 cut(s)	647
Bst2BI	CACGAG	1 cut(s)	270
Bst4CI	ACNGT	5 cut(s)	151, 304, 347, 472, 1490
Bst6I	CTCTTC	1 cut(s)	1376
BstACI	GRCGYC	3 cut(s)	6, 30, 100
BstAFI	CTTAAG	1 cut(s)	1280
BstAUI	TGTACA	1 cut(s)	567
BstC8I	GCNNGC	3 cut(s)	286, 560, 783
BstDEI	CTNAG	9 cut(s)	314, 455, 548, 589, 900, 1086, 1428, 1744, 1773
BstDSI	CCRYGG	3 cut(s)	681, 804, 915
BstF5I	GGATG	9 cut(s)	514, 604, 772, 847, 925, 1009, 1065, 1301, 1708
BstFNI	CGCG	2 cut(s)	917, 1740
BstKTI	GATC	5 cut(s)	269, 525, 1328, 1482, 1539
BstMAI	GTCTC	2 cut(s)	1398, 1747
BstMBI	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
BstMWI	GCNNNNNNNGC	6 cut(s)	59, 184, 245, 686, 767, 1241
BstSCI	CCNGG	2 cut(s)	154, 198
BstSFI	CTRYAG	2 cut(s)	84, 955
BstUI	CGCG	2 cut(s)	917, 1740
BstV1I	GCAGC	5 cut(s)	98, 580, 664, 1272, 1735
BstV2I	GAAGAC	1 cut(s)	1469
BsuI	GTATCC	2 cut(s)	251, 1352
BsuRI	GGCC	1 cut(s)	713
BtgI	CCRYGG	3 cut(s)	681, 804, 915
BtgZI	GCGATG	1 cut(s)	175
BtsCI	GGATG	9 cut(s)	514, 604, 772, 847, 925, 1009, 1065, 1301, 1708
BtsI	GCAGTG	2 cut(s)	816, 885
BtsIMutI	CAGTG	5 cut(s)	147, 352, 816, 885, 1554
BveI	ACCTGC	1 cut(s)	926
Cac8I	GCNNGC	3 cut(s)	286, 560, 783
CciI	TCATGA	2 cut(s)	514, 1516
Cfr10I	RCCGGY	1 cut(s)	239
Cfr13I	GGNCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
Cfr42I	CCGCGG	1 cut(s)	918
CseI	GACGC	3 cut(s)	19, 277, 1729
Csp6I	GTAC	3 cut(s)	343, 568, 1303
CspCI	CAANNNNNGTGG	2 cut(s)	1471, 1506
CviAII	CATG	2 cut(s)	515, 1517
CviQI	GTAC	3 cut(s)	343, 568, 1303
DdeI	CTNAG	9 cut(s)	314, 455, 548, 589, 900, 1086, 1428, 1744, 1773
DpnI	GATC	5 cut(s)	268, 524, 1327, 1481, 1538
DpnII	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
Eam1104I	CTCTTC	1 cut(s)	1376
EarI	CTCTTC	1 cut(s)	1376
Ecl136II	GAGCTC	1 cut(s)	205
Eco130I	CCWWGG	1 cut(s)	647
Eco24I	GRGCYC	2 cut(s)	207, 1736
Eco47I	GGWCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
Eco53kI	GAGCTC	1 cut(s)	205
Eco57I	CTGAAG	3 cut(s)	777, 1057, 1164
EcoICRI	GAGCTC	1 cut(s)	205
EcoO109I	RGGNCCY	1 cut(s)	380
EcoRI	GAATTC	1 cut(s)	1067
EcoT14I	CCWWGG	1 cut(s)	647
EcoT38I	GRGCYC	2 cut(s)	207, 1736
ErhI	CCWWGG	1 cut(s)	647
Esp3I	CGTCTC	2 cut(s)	1398, 1747
FaeI	CATG	2 cut(s)	518, 1520
FalI	AAGNNNNNCTT	6 cut(s)	1162, 1194, 1656, 1688, 1707, 1739
FaqI	GGGAC	4 cut(s)	614, 723, 821, 1124
FatI	CATG	2 cut(s)	514, 1516
FauI	CCCGC	1 cut(s)	119
FbaI	TGATCA	2 cut(s)	1479, 1536
FblI	GTMKAC	1 cut(s)	16
Fnu4HI	GCNGC	7 cut(s)	63, 87, 249, 594, 678, 1286, 1749
FokI	GGATG	9 cut(s)	521, 591, 779, 834, 932, 996, 1072, 1308, 1715
FriOI	GRGCYC	2 cut(s)	207, 1736
Fsp4HI	GCNGC	7 cut(s)	63, 87, 249, 594, 678, 1286, 1749
FspBI	CTAG	4 cut(s)	618, 762, 782, 1355
GluI	GCNGC	7 cut(s)	63, 87, 249, 594, 678, 1286, 1749
HaeIII	GGCC	1 cut(s)	713
HapII	CCGG	6 cut(s)	129, 156, 200, 240, 409, 1376
HgaI	GACGC	3 cut(s)	19, 277, 1729
Hin1I	GRCGYC	3 cut(s)	6, 30, 100
Hin1II	CATG	2 cut(s)	518, 1520
HpaII	CCGG	6 cut(s)	129, 156, 200, 240, 409, 1376
HphI	GGTGA	8 cut(s)	127, 170, 292, 505, 1105, 1265, 1595, 1621
Hpy166II	GTNNAC	6 cut(s)	17, 311, 434, 496, 568, 1696
Hpy188I	TCNGA	6 cut(s)	11, 549, 901, 1059, 1212, 1275
Hpy188III	TCNNGA	8 cut(s)	292, 515, 985, 1013, 1430, 1477, 1517, 1540
Hpy8I	GTNNAC	6 cut(s)	17, 311, 434, 496, 568, 1696
Hpy99I	CGWCG	2 cut(s)	35, 293
HpyAV	CCTTC	2 cut(s)	1139, 1580
HpyCH4III	ACNGT	5 cut(s)	151, 304, 347, 472, 1490
HpyCH4IV	ACGT	3 cut(s)	6, 100, 1434
HpyCH4V	TGCA	4 cut(s)	86, 1009, 1164, 1318
HpyF10VI	GCNNNNNNNGC	6 cut(s)	59, 184, 245, 686, 767, 1241
HpyF3I	CTNAG	9 cut(s)	314, 455, 548, 589, 900, 1086, 1428, 1744, 1773
HpySE526I	ACGT	3 cut(s)	6, 100, 1434
Hsp92I	GRCGYC	3 cut(s)	6, 30, 100
Hsp92II	CATG	2 cut(s)	518, 1520
Ksp22I	TGATCA	2 cut(s)	1479, 1536
KspI	CCGCGG	1 cut(s)	918
Kzo9I	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
LmnI	GCTCC	6 cut(s)	58, 83, 202, 778, 875, 965
Lsp1109I	GCAGC	5 cut(s)	98, 580, 664, 1272, 1735
LweI	GCATC	9 cut(s)	499, 585, 757, 778, 804, 910, 1018, 1206, 1558
MaeI	CTAG	4 cut(s)	618, 762, 782, 1355
MaeII	ACGT	3 cut(s)	6, 100, 1434
MalI	GATC	5 cut(s)	268, 524, 1327, 1481, 1538
MbiI	CCGCTC	1 cut(s)	53
MboI	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
MboII	GAAGA	9 cut(s)	407, 922, 937, 1063, 1225, 1393, 1465, 1474, 1672
MfeI	CAATTG	2 cut(s)	1267, 1329
MhlI	GDGCHC	3 cut(s)	207, 887, 1736
MluCI	AATT	4 cut(s)	1067, 1267, 1329, 1543
MlyI	GAGTC	7 cut(s)	41, 85, 141, 285, 306, 920, 1345
MmeI	TCCRAC	3 cut(s)	355, 414, 1225
MseI	TTAA	2 cut(s)	1281, 1641
MslI	CAYNNNNRTG	5 cut(s)	1004, 1581, 1607, 1685, 1701
MspA1I	CMGCKG	4 cut(s)	582, 917, 1235, 1748
MspCI	CTTAAG	1 cut(s)	1280
MspI	CCGG	6 cut(s)	129, 156, 200, 240, 409, 1376
MspR9I	CCNGG	2 cut(s)	156, 200
MunI	CAATTG	2 cut(s)	1267, 1329
MvnI	CGCG	2 cut(s)	917, 1740
MwoI	GCNNNNNNNGC	6 cut(s)	59, 184, 245, 686, 767, 1241
NciI	CCSGG	2 cut(s)	156, 200
NdeII	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
NheI	GCTAGC	1 cut(s)	781
NlaIII	CATG	2 cut(s)	518, 1520
NlaIV	GGNNCC	2 cut(s)	79, 739
NmeAIII	GCCGAG	1 cut(s)	54
NmuCI	GTSAC	4 cut(s)	101, 133, 292, 1420
OliI	CACNNNNGTG	1 cut(s)	1607
PagI	TCATGA	2 cut(s)	514, 1516
PcsI	WCGNNNNNNNCGW	1 cut(s)	285
PfeI	GAWTC	6 cut(s)	412, 1131, 1185, 1256, 1447, 1513
PkrI	GCNGC	7 cut(s)	64, 88, 250, 595, 679, 1287, 1750
PleI	GAGTC	7 cut(s)	41, 85, 140, 284, 306, 919, 1345
PpsI	GAGTC	7 cut(s)	41, 85, 140, 284, 306, 919, 1345
PpuMI	RGGWCCY	1 cut(s)	380
Psp124BI	GAGCTC	1 cut(s)	207
Psp5II	RGGWCCY	1 cut(s)	380
PspN4I	GGNNCC	2 cut(s)	79, 739
PspPI	GGNCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
PspPPI	RGGWCCY	1 cut(s)	380
PstI	CTGCAG	1 cut(s)	88
PvuII	CAGCTG	2 cut(s)	582, 1748
RsaI	GTAC	3 cut(s)	344, 569, 1304
RsaNI	GTAC	3 cut(s)	343, 568, 1303
RseI	CAYNNNNRTG	5 cut(s)	1004, 1581, 1607, 1685, 1701
SacI	GAGCTC	1 cut(s)	207
SacII	CCGCGG	1 cut(s)	918
SaqAI	TTAA	2 cut(s)	1281, 1641
SatI	GCNGC	7 cut(s)	63, 87, 249, 594, 678, 1286, 1749
Sau3AI	GATC	5 cut(s)	266, 522, 1325, 1479, 1536
Sau96I	GGNCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
SchI	GAGTC	7 cut(s)	41, 85, 141, 285, 306, 920, 1345
ScrFI	CCNGG	2 cut(s)	156, 200
SduI	GDGCHC	3 cut(s)	207, 887, 1736
SfaNI	GCATC	9 cut(s)	499, 585, 757, 778, 804, 910, 1018, 1206, 1558
SfcI	CTRYAG	2 cut(s)	84, 955
Sfr303I	CCGCGG	1 cut(s)	918
SgrBI	CCGCGG	1 cut(s)	918
SinI	GGWCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
SmiMI	CAYNNNNRTG	5 cut(s)	1004, 1581, 1607, 1685, 1701
SmlI	CTYRAG	4 cut(s)	983, 1280, 1365, 1724
SmoI	CTYRAG	4 cut(s)	983, 1280, 1365, 1724
Sse9I	AATT	4 cut(s)	1067, 1267, 1329, 1543
SsiI	CCGC	8 cut(s)	51, 60, 63, 112, 249, 915, 917, 1233
SspMI	CTAG	4 cut(s)	618, 762, 782, 1355
SstI	GAGCTC	1 cut(s)	207
StyD4I	CCNGG	2 cut(s)	154, 198
StyI	CCWWGG	1 cut(s)	647
TaaI	ACNGT	5 cut(s)	151, 304, 347, 472, 1490
TaiI	ACGT	3 cut(s)	9, 103, 1437
TaqI	TCGA	4 cut(s)	265, 525, 909, 1566
TasI	AATT	4 cut(s)	1067, 1267, 1329, 1543
TatI	WGTACW	1 cut(s)	567
TauI	GCSGC	2 cut(s)	65, 251
TfiI	GAWTC	6 cut(s)	412, 1131, 1185, 1256, 1447, 1513
Tru1I	TTAA	2 cut(s)	1281, 1641
Tru9I	TTAA	2 cut(s)	1281, 1641
TscAI	CASTG	5 cut(s)	154, 352, 816, 885, 1561
TseFI	GTSAC	4 cut(s)	101, 133, 292, 1420
TseI	GCWGC	5 cut(s)	86, 593, 677, 1285, 1748
Tsp45I	GTSAC	4 cut(s)	101, 133, 292, 1420
TspDTI	ATGAA	8 cut(s)	407, 531, 847, 1242, 1387, 1505, 1565, 1669
TspGWI	ACGGA	3 cut(s)	952, 1422, 1470
TspRI	CASTG	5 cut(s)	154, 352, 816, 885, 1561
Vha464I	CTTAAG	1 cut(s)	1280
VpaK11BI	GGWCC	6 cut(s)	380, 737, 938, 1037, 1523, 1599
XapI	RAATTY	1 cut(s)	1067
XcmI	CCANNNNNNNNNTGG	1 cut(s)	748
XmiI	GTMKAC	1 cut(s)	16
XspI	CTAG	4 cut(s)	618, 762, 782, 1355
ZraI	GACGTC	2 cut(s)	7, 101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.