Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

25333520 .. 25336407

Gene Structure 2888 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1173200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGCTTTTTGAGAATCTTCCATTGATTTGTATAGTTGGAGGGCCGAACTCGAACGATTATGAGACGAACAGGATTCTTCACCATACTATTGGGTTGCCGGACTTTAGCCAAGAGCTGAGAGGCTTTCAGACTGTAACTTGCTATCAGGCTGTGGTGAATAATCTGGAAGATGCGCATGAAATGATCGATACCGCAATTTCAACTGCTTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTCCTCATCCTACATTTAGCCGGGAGCCTGTTCCATTTTCTTTATCTCCAAAATTAAGCAATAATTTGGGCTTAGAGGCTGCGGTGGAGGCGGCTGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTTGGTGGGCCTAAACTTCGAGTTGCACATGCCGGCGATGCCTTTGTTGAACTTGCTGATGCTAGTGGTTATGCTCTGGCTGTCATGCCATCTGCAAAGGGGTTTGTACCAGAGCACCACCCCCATTTTATTGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGCGCCGAGATAGTGGAGTCCGCAGATGCATACTTGTTTGCTGGACCGATTTTCAATGACTACAGCTCTGTTGGATACTCTTTGCTTATCAAGAAGGAGAAGGCCATTGTGGTGCAGCCTGATCGCGTGACCATAGCAAATGGCCCTTCATTTGGTTGTGTTCTTATGAAGGATTTCCTCAGAGCTCTTGCAAAGAAGCTCAGGCACAACAACACTGCTCATGAGAACTACCGCAGGATCTTTGTTCCCGATGGACACCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAACGTTCTGTTCCACCACATCCAGAATATGCTGTCAAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGGCTGCGGGTATGAGTTCCAAATGCAATATGGATCAATCGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAGGCTGTTCCTCAGAAGCGTGTGATTGCTTTCGTCGGTGACGGGAGTTTCCAGGTGACTGCTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGACCATCATCTTCCTGATAAACAACGGCGGGTACACAATTGAGGTGGAGATCCATGACGGACCCTACAATGTGATCAAGAACTGGAACTACACTGGACTGGTTGATGCCATCCACAATGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATCGAAGCGATTGAGACTGCAACCGGGGCAAAGAAGGATAGCTTGTGCTTCATAGAGGTGATTGTCCACAAGGATGATACCAGCAAAAAGTTGCTTGAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCCAACCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

480

Amino Acids

52.41

Weight (kDa)

6.15

Isoelectric Point (pI)

27.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_M	PF00205	115 - 232	1.3e-22	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	326 - 450	3.2e-16	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	174
AccII	CGCG	1 cut(s)	681
AciI	CCGC	8 cut(s)	192, 341, 350, 576, 787, 960, 1152, 1420
AclI	AACGTT	1 cut(s)	852
AclWI	GGATC	4 cut(s)	800, 994, 1102, 1168
AcsI	RAATTY	1 cut(s)	258
AfaI	GTAC	2 cut(s)	498, 1157
AfiI	CCNNNNNNNGG	2 cut(s)	707, 1243
AgsI	TTSAA	5 cut(s)	201, 211, 367, 440, 610
AjnI	CCWGG	1 cut(s)	1077
AluBI	AGCT	7 cut(s)	115, 243, 621, 740, 754, 1288, 1332
AluI	AGCT	7 cut(s)	115, 243, 621, 740, 754, 1288, 1332
Alw21I	GWGCWC	3 cut(s)	507, 548, 742
Alw26I	GTCTC	3 cut(s)	56, 902, 1298
AlwI	GGATC	4 cut(s)	800, 994, 1102, 1168
AlwNI	CAGNNNCTG	1 cut(s)	940
AoxI	GGCC	4 cut(s)	41, 398, 657, 697
ApeKI	GCWGC	6 cut(s)	243, 338, 353, 670, 957, 1409
ApoI	RAATTY	1 cut(s)	258
Asp700I	GAANNNNTTC	1 cut(s)	728
AspLEI	GCGC	2 cut(s)	175, 560
AspS9I	GGNCC	6 cut(s)	41, 398, 599, 698, 1184, 1270
AsuC2I	CCSGG	2 cut(s)	281, 1315
AsuHPI	GGTGA	5 cut(s)	71, 166, 1076, 1093, 1360
AvaII	GGWCC	3 cut(s)	599, 1184, 1270
BanII	GRGCYC	1 cut(s)	742
BbsI	GAAGAC	1 cut(s)	1130
Bbv12I	GWGCWC	3 cut(s)	507, 548, 742
BbvI	GCAGC	6 cut(s)	230, 325, 340, 682, 944, 1396
BccI	CCATC	4 cut(s)	487, 800, 1136, 1241
BceAI	ACGGC	1 cut(s)	1165
BcgI	CGANNNNNNTGC	2 cut(s)	659, 693
BciT130I	CCWGG	1 cut(s)	1079
BciVI	GTATCC	1 cut(s)	623
BclI	TGATCA	1 cut(s)	1197
BcnI	CCSGG	2 cut(s)	281, 1315
BcoDI	GTCTC	3 cut(s)	56, 902, 1298
BfaI	CTAG	2 cut(s)	453, 1398
BfmI	CTRYAG	2 cut(s)	354, 616
BfuI	GTATCC	1 cut(s)	623
BisI	GCNGC	8 cut(s)	244, 339, 351, 354, 671, 958, 1410, 1420
BlsI	GCNGC	8 cut(s)	245, 340, 352, 355, 672, 959, 1411, 1421
Bme1390I	CCNGG	3 cut(s)	281, 1079, 1315
Bme18I	GGWCC	3 cut(s)	599, 1184, 1270
BmgT120I	GGNCC	6 cut(s)	41, 398, 599, 698, 1184, 1270
BmiI	GGNNCC	2 cut(s)	285, 1186
BmrFI	CCNGG	3 cut(s)	281, 1079, 1315
BmrI	ACTGGG	1 cut(s)	541
BmsI	GCATC	5 cut(s)	160, 418, 439, 571, 1219
BmuI	ACTGGG	1 cut(s)	541
BpiI	GAAGAC	1 cut(s)	1130
Bpu10I	CCTNAGC	2 cut(s)	755, 1026
BpuEI	CTTGAG	2 cut(s)	1074, 1406
BpuMI	CCSGG	2 cut(s)	281, 1315
Bsa29I	ATCGAT	1 cut(s)	186
BsaJI	CCNNGG	2 cut(s)	1266, 1314
BsaXI	ACNNNNNCTCC	2 cut(s)	1238, 1268
Bsc4I	CCNNNNNNNGG	2 cut(s)	707, 1243
Bse118I	RCCGGY	3 cut(s)	382, 422, 949
Bse1I	ACTGG	4 cut(s)	536, 1211, 1222, 1227
BseBI	CCWGG	1 cut(s)	1079
BseCI	ATCGAT	1 cut(s)	186
BseDI	CCNNGG	2 cut(s)	1266, 1314
BseGI	GGATG	4 cut(s)	265, 867, 1233, 1369
BseLI	CCNNNNNNNGG	2 cut(s)	707, 1243
BseMII	CTCAG	6 cut(s)	107, 748, 769, 897, 1040, 1052
BseNI	ACTGG	4 cut(s)	536, 1211, 1222, 1227
BseRI	GAGGAG	1 cut(s)	1298
BseXI	GCAGC	6 cut(s)	230, 325, 340, 682, 944, 1396
BsgI	GTGCAG	2 cut(s)	689, 810
Bsh1236I	CGCG	1 cut(s)	681
BshFI	GGCC	4 cut(s)	43, 400, 659, 699
BshVI	ATCGAT	1 cut(s)	186
BsiHKAI	GWGCWC	3 cut(s)	507, 548, 742
BsiSI	CCGG	6 cut(s)	98, 280, 383, 423, 950, 1314
BslFI	GGGAC	1 cut(s)	929
BslI	CCNNNNNNNGG	2 cut(s)	707, 1243
BsmAI	GTCTC	3 cut(s)	56, 902, 1298
BsmBI	CGTCTC	1 cut(s)	56
BsmFI	GGGAC	1 cut(s)	929
BsnI	GGCC	4 cut(s)	43, 400, 659, 699
Bsp1286I	GDGCHC	3 cut(s)	507, 548, 742
Bsp143I	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
BspACI	CCGC	8 cut(s)	192, 341, 350, 576, 787, 960, 1152, 1420
BspANI	GGCC	4 cut(s)	43, 400, 659, 699
BspCNI	CTCAG	6 cut(s)	108, 747, 768, 898, 1039, 1051
BspDI	ATCGAT	1 cut(s)	186
BspFNI	CGCG	1 cut(s)	681
BspHI	TCATGA	1 cut(s)	775
BspLI	GGNNCC	2 cut(s)	285, 1186
BspMAI	CTGCAG	1 cut(s)	358
BspPI	GGATC	4 cut(s)	800, 994, 1102, 1168
BsrFI	RCCGGY	3 cut(s)	382, 422, 949
BsrI	ACTGG	4 cut(s)	536, 1211, 1222, 1227
BssAI	RCCGGY	3 cut(s)	382, 422, 949
BssECI	CCNNGG	2 cut(s)	1266, 1314
BssMI	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
BssT1I	CCWWGG	1 cut(s)	1266
Bst2UI	CCWGG	1 cut(s)	1079
Bst4CI	ACNGT	1 cut(s)	133
Bst6I	CTCTTC	1 cut(s)	1275
BstAPI	GCANNNNNTGC	1 cut(s)	243
BstC8I	GCNNGC	4 cut(s)	221, 424, 951, 955
BstDEI	CTNAG	8 cut(s)	116, 331, 734, 755, 906, 1026, 1038, 1434
BstF5I	GGATG	4 cut(s)	265, 867, 1233, 1369
BstFNI	CGCG	1 cut(s)	681
BstHHI	GCGC	2 cut(s)	175, 560
BstKTI	GATC	8 cut(s)	186, 679, 795, 989, 1110, 1176, 1200, 1293
BstMAI	GTCTC	3 cut(s)	56, 902, 1298
BstMBI	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
BstMWI	GCNNNNNNNGC	6 cut(s)	243, 347, 428, 557, 902, 1316
BstNI	CCWGG	1 cut(s)	1079
BstNSI	RCATGY	1 cut(s)	422
BstSCI	CCNGG	3 cut(s)	279, 1077, 1313
BstSFI	CTRYAG	2 cut(s)	354, 616
BstUI	CGCG	1 cut(s)	681
BstV1I	GCAGC	6 cut(s)	230, 325, 340, 682, 944, 1396
BstV2I	GAAGAC	1 cut(s)	1130
BstX2I	RGATCY	2 cut(s)	792, 1173
BstXI	CCANNNNNNTGG	2 cut(s)	89, 521
BstYI	RGATCY	2 cut(s)	792, 1173
Bsu15I	ATCGAT	1 cut(s)	186
BsuI	GTATCC	1 cut(s)	623
BsuRI	GGCC	4 cut(s)	43, 400, 659, 699
BsuTUI	ATCGAT	1 cut(s)	186
BtgZI	GCGATG	1 cut(s)	441
BtsCI	GGATG	4 cut(s)	265, 867, 1233, 1369
BtsI	GCAGTG	3 cut(s)	381, 546, 768
BtsIMutI	CAGTG	4 cut(s)	381, 546, 768, 1215
Cac8I	GCNNGC	4 cut(s)	221, 424, 951, 955
CaiI	CAGNNNCTG	1 cut(s)	940
CciI	TCATGA	1 cut(s)	775
CfoI	GCGC	2 cut(s)	175, 560
Cfr10I	RCCGGY	3 cut(s)	382, 422, 949
Cfr13I	GGNCC	6 cut(s)	41, 398, 599, 698, 1184, 1270
ClaI	ATCGAT	1 cut(s)	186
Csp6I	GTAC	2 cut(s)	497, 1156
CspCI	CAANNNNNGTGG	2 cut(s)	1149, 1184
CviAII	CATG	7 cut(s)	176, 419, 475, 776, 921, 1105, 1178
CviQI	GTAC	2 cut(s)	497, 1156
DdeI	CTNAG	8 cut(s)	116, 331, 734, 755, 906, 1026, 1038, 1434
DpnI	GATC	8 cut(s)	185, 678, 794, 988, 1109, 1175, 1199, 1292
DpnII	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
Eam1104I	CTCTTC	1 cut(s)	1275
EarI	CTCTTC	1 cut(s)	1275
Ecl136II	GAGCTC	1 cut(s)	740
Eco130I	CCWWGG	1 cut(s)	1266
Eco24I	GRGCYC	1 cut(s)	742
Eco47I	GGWCC	3 cut(s)	599, 1184, 1270
Eco53kI	GAGCTC	1 cut(s)	740
EcoICRI	GAGCTC	1 cut(s)	740
EcoRI	GAATTC	1 cut(s)	258
EcoRII	CCWGG	1 cut(s)	1077
EcoT14I	CCWWGG	1 cut(s)	1266
EcoT22I	ATGCAT	1 cut(s)	586
EcoT38I	GRGCYC	1 cut(s)	742
ErhI	CCWWGG	1 cut(s)	1266
Esp3I	CGTCTC	1 cut(s)	56
FaeI	CATG	7 cut(s)	179, 422, 478, 779, 924, 1108, 1181
FalI	AAGNNNNNCTT	2 cut(s)	1316, 1348
FaqI	GGGAC	1 cut(s)	929
FatI	CATG	7 cut(s)	175, 418, 474, 775, 920, 1104, 1177
FauI	CCCGC	2 cut(s)	953, 1145
FbaI	TGATCA	1 cut(s)	1197
Fnu4HI	GCNGC	8 cut(s)	244, 339, 351, 354, 671, 958, 1410, 1420
FokI	GGATG	4 cut(s)	252, 854, 1220, 1376
FriOI	GRGCYC	1 cut(s)	742
Fsp4HI	GCNGC	8 cut(s)	244, 339, 351, 354, 671, 958, 1410, 1420
FspAI	RTGCGCAY	1 cut(s)	174
FspBI	CTAG	2 cut(s)	453, 1398
FspI	TGCGCA	1 cut(s)	174
GlaI	GCGC	2 cut(s)	174, 559
GluI	GCNGC	8 cut(s)	244, 339, 351, 354, 671, 958, 1410, 1420
HaeIII	GGCC	4 cut(s)	43, 400, 659, 699
HapII	CCGG	6 cut(s)	98, 280, 383, 423, 950, 1314
HhaI	GCGC	2 cut(s)	175, 560
Hin1II	CATG	7 cut(s)	179, 422, 478, 779, 924, 1108, 1181
Hin6I	GCGC	2 cut(s)	173, 558
HinP1I	GCGC	2 cut(s)	173, 558
HincII	GTYRAC	1 cut(s)	850
HindII	GTYRAC	1 cut(s)	850
HinfI	GANTC	4 cut(s)	13, 73, 572, 917
HpaI	GTTAAC	1 cut(s)	850
HpaII	CCGG	6 cut(s)	98, 280, 383, 423, 950, 1314
HphI	GGTGA	5 cut(s)	71, 166, 1076, 1093, 1360
Hpy166II	GTNNAC	4 cut(s)	850, 1101, 1158, 1357
Hpy188I	TCNGA	4 cut(s)	129, 737, 1041, 1112
Hpy188III	TCNNGA	9 cut(s)	164, 364, 646, 776, 803, 871, 1091, 1138, 1201
Hpy8I	GTNNAC	4 cut(s)	850, 1101, 1158, 1357
Hpy99I	CGWCG	1 cut(s)	1064
HpyAV	CCTTC	5 cut(s)	643, 649, 711, 718, 1318
HpyCH4III	ACNGT	1 cut(s)	133
HpyCH4IV	ACGT	1 cut(s)	852
HpyF10VI	GCNNNNNNNGC	6 cut(s)	243, 347, 428, 557, 902, 1316
HpyF3I	CTNAG	8 cut(s)	116, 331, 734, 755, 906, 1026, 1038, 1434
HpySE526I	ACGT	1 cut(s)	852
Hsp92II	CATG	7 cut(s)	179, 422, 478, 779, 924, 1108, 1181
HspAI	GCGC	2 cut(s)	173, 558
KroI	GCCGGC	2 cut(s)	422, 949
KroNI	GCCGGC	2 cut(s)	424, 951
Ksp22I	TGATCA	1 cut(s)	1197
KspAI	GTTAAC	1 cut(s)	850
Kzo9I	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
LmnI	GCTCC	3 cut(s)	283, 1007, 1285
Lsp1109I	GCAGC	6 cut(s)	230, 325, 340, 682, 944, 1396
LweI	GCATC	5 cut(s)	160, 418, 439, 571, 1219
MaeI	CTAG	2 cut(s)	453, 1398
MaeII	ACGT	1 cut(s)	852
MaeIII	GTNAC	4 cut(s)	133, 682, 1064, 1081
MalI	GATC	8 cut(s)	185, 678, 794, 988, 1109, 1175, 1199, 1292
MboI	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
MboII	GAAGA	6 cut(s)	8, 68, 179, 1126, 1135, 1292
MfeI	CAATTG	1 cut(s)	1161
MflI	RGATCY	2 cut(s)	792, 1173
MhlI	GDGCHC	3 cut(s)	507, 548, 742
MluCI	AATT	6 cut(s)	195, 258, 311, 322, 944, 1161
MlyI	GAGTC	2 cut(s)	581, 911
MmeI	TCCRAC	3 cut(s)	16, 607, 985
Mph1103I	ATGCAT	1 cut(s)	586
MroNI	GCCGGC	2 cut(s)	422, 949
MroXI	GAANNNNTTC	1 cut(s)	728
MseI	TTAA	3 cut(s)	314, 849, 927
MslI	CAYNNNNRTG	3 cut(s)	665, 1346, 1362
MspI	CCGG	6 cut(s)	98, 280, 383, 423, 950, 1314
MspR9I	CCNGG	3 cut(s)	281, 1079, 1315
MunI	CAATTG	1 cut(s)	1161
MvaI	CCWGG	1 cut(s)	1079
MvnI	CGCG	1 cut(s)	681
MwoI	GCNNNNNNNGC	6 cut(s)	243, 347, 428, 557, 902, 1316
NaeI	GCCGGC	2 cut(s)	424, 951
NciI	CCSGG	2 cut(s)	281, 1315
NdeII	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
NgoMIV	GCCGGC	2 cut(s)	422, 949
NlaIII	CATG	7 cut(s)	179, 422, 478, 779, 924, 1108, 1181
NlaIV	GGNNCC	2 cut(s)	285, 1186
NmeAIII	GCCGAG	1 cut(s)	586
NmuCI	GTSAC	3 cut(s)	682, 1064, 1081
NsbI	TGCGCA	1 cut(s)	174
NsiI	ATGCAT	1 cut(s)	586
NspI	RCATGY	1 cut(s)	422
PagI	TCATGA	1 cut(s)	775
PdiI	GCCGGC	2 cut(s)	424, 951
PdmI	GAANNNNTTC	1 cut(s)	728
PfeI	GAWTC	2 cut(s)	13, 73
PkrI	GCNGC	8 cut(s)	245, 340, 352, 355, 672, 959, 1411, 1421
PleI	GAGTC	2 cut(s)	580, 911
PpsI	GAGTC	2 cut(s)	580, 911
Psp124BI	GAGCTC	1 cut(s)	742
Psp1406I	AACGTT	1 cut(s)	852
Psp6I	CCWGG	1 cut(s)	1077
PspGI	CCWGG	1 cut(s)	1077
PspN4I	GGNNCC	2 cut(s)	285, 1186
PspPI	GGNCC	6 cut(s)	41, 398, 599, 698, 1184, 1270
PsrI	GAACNNNNNNTAC	2 cut(s)	1196, 1228
PstI	CTGCAG	1 cut(s)	358
PstNI	CAGNNNCTG	1 cut(s)	940
PsuI	RGATCY	2 cut(s)	792, 1173
RsaI	GTAC	2 cut(s)	498, 1157
RsaNI	GTAC	2 cut(s)	497, 1156
RseI	CAYNNNNRTG	3 cut(s)	665, 1346, 1362
SacI	GAGCTC	1 cut(s)	742
SaqAI	TTAA	3 cut(s)	314, 849, 927
SatI	GCNGC	8 cut(s)	244, 339, 351, 354, 671, 958, 1410, 1420
Sau3AI	GATC	8 cut(s)	183, 676, 792, 986, 1107, 1173, 1197, 1290
Sau96I	GGNCC	6 cut(s)	41, 398, 599, 698, 1184, 1270
SchI	GAGTC	2 cut(s)	581, 911
ScrFI	CCNGG	3 cut(s)	281, 1079, 1315
SduI	GDGCHC	3 cut(s)	507, 548, 742
SfaNI	GCATC	5 cut(s)	160, 418, 439, 571, 1219
SfcI	CTRYAG	2 cut(s)	354, 616
SinI	GGWCC	3 cut(s)	599, 1184, 1270
SmiMI	CAYNNNNRTG	3 cut(s)	665, 1346, 1362
SmlI	CTYRAG	2 cut(s)	1089, 1385
SmoI	CTYRAG	2 cut(s)	1089, 1385
Sse9I	AATT	6 cut(s)	195, 258, 311, 322, 944, 1161
SsiI	CCGC	8 cut(s)	192, 341, 350, 576, 787, 960, 1152, 1420
SspMI	CTAG	2 cut(s)	453, 1398
SstI	GAGCTC	1 cut(s)	742
StyD4I	CCNGG	3 cut(s)	279, 1077, 1313
StyI	CCWWGG	1 cut(s)	1266
TaaI	ACNGT	1 cut(s)	133
TaiI	ACGT	1 cut(s)	855
TaqI	TCGA	4 cut(s)	50, 186, 409, 1293
TaqII	GACCGA	1 cut(s)	616
TasI	AATT	6 cut(s)	195, 258, 311, 322, 944, 1161
TauI	GCSGC	2 cut(s)	353, 1422
TfiI	GAWTC	2 cut(s)	13, 73
Tru1I	TTAA	3 cut(s)	314, 849, 927
Tru9I	TTAA	3 cut(s)	314, 849, 927
TscAI	CASTG	4 cut(s)	381, 553, 775, 1222
TseFI	GTSAC	3 cut(s)	682, 1064, 1081
TseI	GCWGC	6 cut(s)	243, 338, 353, 670, 957, 1409
Tsp45I	GTSAC	3 cut(s)	682, 1064, 1081
TspDTI	ATGAA	4 cut(s)	192, 693, 737, 1330
TspGWI	ACGGA	2 cut(s)	1197, 1262
TspRI	CASTG	4 cut(s)	381, 553, 775, 1222
VpaK11BI	GGWCC	3 cut(s)	599, 1184, 1270
XapI	RAATTY	1 cut(s)	258
XceI	RCATGY	1 cut(s)	422
XcmI	CCANNNNNNNNNTGG	1 cut(s)	980
XmnI	GAANNNNTTC	1 cut(s)	728
XspI	CTAG	2 cut(s)	453, 1398
Zsp2I	ATGCAT	1 cut(s)	586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.