Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Reverse (-)

44674628 .. 44683211

Gene Structure 8584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ19971

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGACATCAAAATGGTGTCCTCTCACTCCTCTGCACTTCTATTCCCAAACCCCATCAAACTCATTTCTCAACAACAACCAAAGCCGAAACTGAAAACCTTCTTCTATCAACACCCAAATCCTATCTCAATTAAACTTCGATGTATGGCAGTTAATTCCAAATATGTTCCTAGTCAATCATCCATGGACACCAAGATTGGAAATGAAGTGATAGAACTGTCCAAACTCGCAACAAGCTGTGACGTCTTTGTTGGCGCCCCACAAAACAGCATCCCTTCCTCCTCCACTTCCCCGATCATGTTGTCCAATGCCACATTAGGTGGATACCTAGCTCGGCGACTAGTCCAAATCGGTGTTGCAGACGTGTTCTCTGTTCCCGGTGACTCTAACTTGATCCTTCTAGATCATCTCATCGCCGAACCTGGGCTAACGAATATTGGCTGCTGCAATGAGCTCAACGCTGGCTACGCTGCTGATGGCTACGCACGCTCCCGAGGCATCGGCGCGTGTGTCGTTACTTTCACGGTCGGCGGACTCAGTTTGCTAAATGCCGTAGCCGGGGCGTACAGTGAGAATTTACCGGTGATTTGTATAGTTGGAGGACCAAATTCCAATGACTATGGAGAGTCGACTAACAATATTCTTCACCATACTATTGGGTTACCTGATTTCAGCCAAGAGCTTAGGTGTTTTCAGACCGTCACTTGCTATCAGGCTGTCATAAACAATCTTGAACATGCACGTCAAAGGATCGATACAGCAATTTCAACAGCCCTGAGAGAAAGCAAGCCAGTTTATATCAGCATAAGTTGTAACTTGGTTGGGATTCCTCATCCTAGTTTTAGCCGTGAGCTTTTTCCAATTTCATTGCCTCCAAGATTAAGTAATCGGATGGCCTTAGACGCTGCAGTGGAGGCAGCAGCAGAGTTCCTGAACAAGGCAGTGAAACCAGTTATGGTAGGTGGTCCCGAACTCCGAGTTGCAAAGGCTGGCGATGCCTTTATTGAACTGGCTGAAGCTTCTCGTTATGCCATGGCTGTTCTGCCAGCAGCAAAGGGGCTTGTGCCGGAGAATTACCCCCAATTTATTGGTACATACTGGGGTGTTGCTAGCAGCGCCTTTTGTTCTGAGATAGTTGAGTCCGCAGATGCATACTTGTTTGCTGGACCAATTTTTGACGACCTCACCACTGTCGGGAACTCACTTCCTCTTGATAAAAGTAAATCCATCATCGTGAAGCTCGACCGCGTTATAATAGCAAACAGGCATGAATTCGGGTCCGTTCTCATGAAGGATTTCCTAAAAGCTCTAGCAAAAAGGTTGAAGTGTAATGGCACTTCCTATGAGAACTATAATAGGATTTTTGTTCCTGAGGGGAAACCTCTGAAGTGTGTTCCCAATGAGCCTTTGAGGGTTAACGTTCTGTTCCAACACATCCAGAAGATGTTGTCGAGTCAAACAACATTGGTTGCTGAAGCCGGGGATTCCTGGTGGAATTGCGTGAAACTGAAATTGCCGCCAGGTTGCCAGTGTGAGTTCCAGGTGTTATATGCATCGATTGGTTGGTCAGTTGGAGCCACTCTTGGTTATGCTCAGGCCTCATCACCTACCAAGCGAGTGATCGCTTTCGTTGGTGATGGGAGCTTCCAGATGACTGCCCAAGACGTGTCGACCATGCTGCGAAATGGGCAGAGGAACATCATTTTTCTGATGAACAATGGTGGATACACCGTGGAAGTGGAGATTCATGATGGACCTTACAATGTGATCAAGAACTGGAACTACACTGGATTAGTTGATGCCATCCACAATAAGGAGGGCAAGTGCTGGACAGCCAAGGTGCGGTGTGAAGAGGAATTGGTTGAAGCGATAGAGACAGCAACTGGAGCCAAGAAGGATTGTTTGTGCTTCATTGAGGTGATTCTTCACAAGGACGATACTAGCAAAGAGCTGCTTGCATGCGGGACTAGATTTGCTGCTGCCAATGGCCGCCCACCTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

667

Amino Acids

72.42

Weight (kDa)

7.45

Isoelectric Point (pI)

39.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	105 - 212	1.8e-28	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	304 - 425	5.4e-21	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	515 - 640	3e-15	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1253
AatII	GACGTC	1 cut(s)	246
AccB1I	GGYRCC	1 cut(s)	254
AccI	GTMKAC	2 cut(s)	629, 1670
AccII	CGCG	2 cut(s)	506, 1248
AciI	CCGC	7 cut(s)	531, 1143, 1246, 1517, 1843, 1962, 1990
AclI	AACGTT	1 cut(s)	1419
AclWI	GGATC	2 cut(s)	388, 758
AcoI	YGGCCR	1 cut(s)	1987
AcsI	RAATTY	3 cut(s)	574, 607, 1271
AcuI	CTGAAG	3 cut(s)	1035, 1406, 1494
AcyI	GRCGYC	2 cut(s)	243, 255
AfaI	GTAC	2 cut(s)	566, 1093
AfiI	CCNNNNNNNGG	6 cut(s)	423, 558, 937, 1194, 1813, 1842
AflIII	ACRYGT	2 cut(s)	363, 1665
AgeI	ACCGGT	1 cut(s)	580
AgsI	TTSAA	5 cut(s)	734, 768, 1007, 1324, 1865
AhlI	ACTAGT	1 cut(s)	340
AjiI	CACGTC	3 cut(s)	364, 743, 1666
AjnI	CCWGG	4 cut(s)	421, 1487, 1519, 1539
Alw21I	GWGCWC	1 cut(s)	456
Alw26I	GTCTC	1 cut(s)	1868
AlwI	GGATC	2 cut(s)	388, 758
AlwNI	CAGNNNCTG	1 cut(s)	1883
Ama87I	CYCGRG	1 cut(s)	492
AoxI	GGCC	3 cut(s)	894, 1596, 1987
ApoI	RAATTY	3 cut(s)	574, 607, 1271
AsiGI	ACCGGT	1 cut(s)	580
Asp700I	GAANNNNTTC	2 cut(s)	98, 1295
AspLEI	GCGC	3 cut(s)	257, 506, 1118
AspS9I	GGNCC	5 cut(s)	602, 965, 1166, 1278, 1754
AsuC2I	CCSGG	3 cut(s)	378, 559, 1480
AsuHPI	GGTGA	7 cut(s)	392, 595, 638, 1177, 1596, 1646, 1930
AsuNHI	GCTAGC	1 cut(s)	1109
AvaI	CYCGRG	1 cut(s)	492
AvaII	GGWCC	5 cut(s)	602, 965, 1166, 1278, 1754
AxyI	CCTNAGG	1 cut(s)	1371
BanI	GGYRCC	1 cut(s)	254
BanII	GRGCYC	1 cut(s)	456
BarI	GAAGNNNNNNTAC	2 cut(s)	1321, 1353
Bbv12I	GWGCWC	1 cut(s)	456
BccI	CCATC	7 cut(s)	62, 470, 886, 1235, 1631, 1745, 1811
BceAI	ACGGC	2 cut(s)	536, 831
BcgI	CGANNNNNNTGC	2 cut(s)	1660, 1694
BciT130I	CCWGG	4 cut(s)	423, 1489, 1521, 1541
BciVI	GTATCC	2 cut(s)	317, 1718
BclI	TGATCA	1 cut(s)	1767
BcnI	CCSGG	3 cut(s)	378, 559, 1480
BcoDI	GTCTC	1 cut(s)	1868
BcuI	ACTAGT	1 cut(s)	340
BfmI	CTRYAG	1 cut(s)	906
BfoI	RGCGCY	2 cut(s)	258, 1119
BfuI	GTATCC	2 cut(s)	317, 1718
Bme1390I	CCNGG	7 cut(s)	378, 423, 559, 1480, 1489, 1521, 1541
Bme18I	GGWCC	5 cut(s)	602, 965, 1166, 1278, 1754
BmeT110I	CYCGRG	1 cut(s)	492
BmgBI	CACGTC	3 cut(s)	364, 743, 1666
BmgT120I	GGNCC	5 cut(s)	602, 965, 1166, 1278, 1754
BmiI	GGNNCC	5 cut(s)	256, 967, 1279, 1576, 1888
BmrFI	CCNGG	7 cut(s)	378, 423, 559, 1480, 1489, 1521, 1541
BmrI	ACTGGG	1 cut(s)	1108
BmsI	GCATC	6 cut(s)	279, 507, 985, 1138, 1562, 1789
BmtI	GCTAGC	1 cut(s)	1113
BmuI	ACTGGG	1 cut(s)	1108
BpmI	CTGGAG	1 cut(s)	1905
Bpu10I	CCTNAGC	2 cut(s)	683, 1593
BpuMI	CCSGG	3 cut(s)	378, 559, 1480
Bsa29I	ATCGAT	2 cut(s)	753, 1556
BsaHI	GRCGYC	2 cut(s)	243, 255
BsaJI	CCNNGG	8 cut(s)	183, 422, 493, 558, 1032, 1479, 1731, 1836
BsaWI	WCCGGW	1 cut(s)	580
BsaXI	ACNNNNNCTCC	4 cut(s)	473, 503, 1808, 1838
Bsc4I	CCNNNNNNNGG	6 cut(s)	423, 558, 937, 1194, 1813, 1842
Bse118I	RCCGGY	1 cut(s)	580
Bse1I	ACTGG	8 cut(s)	791, 950, 1014, 1103, 1528, 1781, 1792, 1888
Bse21I	CCTNAGG	1 cut(s)	1371
Bse3DI	GCAATG	2 cut(s)	454, 866
BseBI	CCWGG	4 cut(s)	423, 1489, 1521, 1541
BseCI	ATCGAT	2 cut(s)	753, 1556
BseDI	CCNNGG	8 cut(s)	183, 422, 493, 558, 1032, 1479, 1731, 1836
BseGI	GGATG	6 cut(s)	179, 270, 832, 897, 1434, 1803
BseLI	CCNNNNNNNGG	6 cut(s)	423, 558, 937, 1194, 1813, 1842
BseMI	GCAATG	2 cut(s)	454, 866
BseMII	CTCAG	5 cut(s)	550, 767, 1119, 1362, 1607
BseNI	ACTGG	8 cut(s)	791, 950, 1014, 1103, 1528, 1781, 1792, 1888
BseRI	GAGGAG	2 cut(s)	19, 271
BsgI	GTGCAG	1 cut(s)	18
Bsh1236I	CGCG	2 cut(s)	506, 1248
Bsh1285I	CGRYCG	2 cut(s)	528, 1246
BshFI	GGCC	3 cut(s)	896, 1598, 1989
BshNI	GGYRCC	1 cut(s)	254
BshTI	ACCGGT	1 cut(s)	580
BshVI	ATCGAT	2 cut(s)	753, 1556
BsiEI	CGRYCG	2 cut(s)	528, 1246
BsiHKAI	GWGCWC	1 cut(s)	456
BsiHKCI	CYCGRG	1 cut(s)	492
BsiSI	CCGG	5 cut(s)	378, 558, 581, 1067, 1479
BslFI	GGGAC	2 cut(s)	951, 1978
BslI	CCNNNNNNNGG	6 cut(s)	423, 558, 937, 1194, 1813, 1842
BsmAI	GTCTC	1 cut(s)	1868
BsmFI	GGGAC	2 cut(s)	951, 1978
BsnI	GGCC	3 cut(s)	896, 1598, 1989
BsoBI	CYCGRG	1 cut(s)	492
Bsp1286I	GDGCHC	1 cut(s)	456
Bsp143I	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
Bsp19I	CCATGG	2 cut(s)	183, 1032
BspACI	CCGC	7 cut(s)	531, 1143, 1246, 1517, 1843, 1962, 1990
BspANI	GGCC	3 cut(s)	896, 1598, 1989
BspCNI	CTCAG	5 cut(s)	549, 768, 1120, 1363, 1606
BspDI	ATCGAT	2 cut(s)	753, 1556
BspFNI	CGCG	2 cut(s)	506, 1248
BspHI	TCATGA	2 cut(s)	1287, 1747
BspLI	GGNNCC	5 cut(s)	256, 967, 1279, 1576, 1888
BspMAI	CTGCAG	1 cut(s)	910
BspOI	GCTAGC	1 cut(s)	1113
BspPI	GGATC	2 cut(s)	388, 758
BspT107I	GGYRCC	1 cut(s)	254
BsrDI	GCAATG	2 cut(s)	454, 866
BsrFI	RCCGGY	1 cut(s)	580
BsrI	ACTGG	8 cut(s)	791, 950, 1014, 1103, 1528, 1781, 1792, 1888
BssAI	RCCGGY	1 cut(s)	580
BssECI	CCNNGG	8 cut(s)	183, 422, 493, 558, 1032, 1479, 1731, 1836
BssMI	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
BssNI	GRCGYC	2 cut(s)	243, 255
BssT1I	CCWWGG	3 cut(s)	183, 1032, 1836
Bst2UI	CCWGG	4 cut(s)	423, 1489, 1521, 1541
Bst4CI	ACNGT	6 cut(s)	219, 526, 569, 700, 1192, 1732
Bst6I	CTCTTC	1 cut(s)	1845
BstACI	GRCGYC	2 cut(s)	243, 255
BstC8I	GCNNGC	8 cut(s)	463, 487, 788, 991, 1047, 1111, 1956, 1960
BstDEI	CTNAG	7 cut(s)	536, 683, 776, 898, 1128, 1371, 1593
BstDSI	CCRYGG	3 cut(s)	183, 1032, 1731
BstEII	GGTNACC	1 cut(s)	660
BstENI	CCTNNNNNAGG	1 cut(s)	935
BstF5I	GGATG	6 cut(s)	179, 270, 832, 897, 1434, 1803
BstFNI	CGCG	2 cut(s)	506, 1248
BstH2I	RGCGCY	2 cut(s)	258, 1119
BstHHI	GCGC	3 cut(s)	257, 506, 1118
BstKTI	GATC	6 cut(s)	297, 396, 406, 753, 1623, 1770
BstMAI	GTCTC	1 cut(s)	1868
BstMBI	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
BstMCI	CGRYCG	2 cut(s)	528, 1246
BstMWI	GCNNNNNNNGC	9 cut(s)	467, 486, 495, 902, 914, 995, 1115, 1687, 1886
BstNI	CCWGG	4 cut(s)	423, 1489, 1521, 1541
BstNSI	RCATGY	2 cut(s)	740, 1962
BstPI	GGTNACC	1 cut(s)	660
BstSCI	CCNGG	7 cut(s)	376, 421, 557, 1478, 1487, 1519, 1539
BstSFI	CTRYAG	1 cut(s)	906
BstUI	CGCG	2 cut(s)	506, 1248
BstXI	CCANNNNNNTGG	2 cut(s)	656, 1088
Bsu15I	ATCGAT	2 cut(s)	753, 1556
Bsu36I	CCTNAGG	1 cut(s)	1371
BsuI	GTATCC	2 cut(s)	317, 1718
BsuRI	GGCC	3 cut(s)	896, 1598, 1989
BsuTUI	ATCGAT	2 cut(s)	753, 1556
BtgI	CCRYGG	3 cut(s)	183, 1032, 1731
BtgZI	GCGATG	2 cut(s)	397, 1008
BtrI	CACGTC	3 cut(s)	364, 743, 1666
BtsCI	GGATG	6 cut(s)	179, 270, 832, 897, 1434, 1803
BtsI	GCAGTG	2 cut(s)	915, 948
BtsIMutI	CAGTG	6 cut(s)	574, 915, 948, 1188, 1535, 1785
Cac8I	GCNNGC	8 cut(s)	463, 487, 788, 991, 1047, 1111, 1956, 1960
CaiI	CAGNNNCTG	1 cut(s)	1883
CciI	TCATGA	2 cut(s)	1287, 1747
CfoI	GCGC	3 cut(s)	257, 506, 1118
Cfr10I	RCCGGY	1 cut(s)	580
Cfr13I	GGNCC	5 cut(s)	602, 965, 1166, 1278, 1754
ClaI	ATCGAT	2 cut(s)	753, 1556
CseI	GACGC	1 cut(s)	911
Csp6I	GTAC	2 cut(s)	565, 1092
CspAI	ACCGGT	1 cut(s)	580
CviAII	CATG	9 cut(s)	184, 298, 737, 1033, 1268, 1288, 1675, 1748, 1959
CviQI	GTAC	2 cut(s)	565, 1092
DdeI	CTNAG	7 cut(s)	536, 683, 776, 898, 1128, 1371, 1593
DinI	GGCGCC	1 cut(s)	256
DpnI	GATC	6 cut(s)	296, 395, 405, 752, 1622, 1769
DpnII	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
EaeI	YGGCCR	1 cut(s)	1987
Eam1104I	CTCTTC	1 cut(s)	1845
EarI	CTCTTC	1 cut(s)	1845
EciI	GGCGGA	1 cut(s)	546
Ecl136II	GAGCTC	1 cut(s)	454
Eco130I	CCWWGG	3 cut(s)	183, 1032, 1836
Eco147I	AGGCCT	1 cut(s)	1598
Eco24I	GRGCYC	1 cut(s)	456
Eco47I	GGWCC	5 cut(s)	602, 965, 1166, 1278, 1754
Eco53kI	GAGCTC	1 cut(s)	454
Eco57I	CTGAAG	3 cut(s)	1035, 1406, 1494
Eco81I	CCTNAGG	1 cut(s)	1371
Eco88I	CYCGRG	1 cut(s)	492
Eco91I	GGTNACC	1 cut(s)	660
EcoICRI	GAGCTC	1 cut(s)	454
EcoNI	CCTNNNNNAGG	1 cut(s)	935
EcoO65I	GGTNACC	1 cut(s)	660
EcoRI	GAATTC	1 cut(s)	1271
EcoRII	CCWGG	4 cut(s)	421, 1487, 1519, 1539
EcoT14I	CCWWGG	3 cut(s)	183, 1032, 1836
EcoT22I	ATGCAT	2 cut(s)	1153, 1555
EcoT38I	GRGCYC	1 cut(s)	456
EgeI	GGCGCC	1 cut(s)	256
EheI	GGCGCC	1 cut(s)	256
ErhI	CCWWGG	3 cut(s)	183, 1032, 1836
FaeI	CATG	9 cut(s)	187, 301, 740, 1036, 1271, 1291, 1678, 1751, 1962
FalI	AAGNNNNNCTT	2 cut(s)	1938, 1970
FaqI	GGGAC	2 cut(s)	951, 1978
FatI	CATG	9 cut(s)	183, 297, 736, 1032, 1267, 1287, 1674, 1747, 1958
FauI	CCCGC	1 cut(s)	1955
FbaI	TGATCA	1 cut(s)	1767
FblI	GTMKAC	2 cut(s)	629, 1670
FokI	GGATG	6 cut(s)	166, 257, 819, 904, 1421, 1790
FriOI	GRGCYC	1 cut(s)	456
GlaI	GCGC	3 cut(s)	256, 505, 1117
GsuI	CTGGAG	1 cut(s)	1905
HaeII	RGCGCY	2 cut(s)	258, 1119
HaeIII	GGCC	3 cut(s)	896, 1598, 1989
HapII	CCGG	5 cut(s)	378, 558, 581, 1067, 1479
HgaI	GACGC	1 cut(s)	911
HhaI	GCGC	3 cut(s)	257, 506, 1118
Hin1I	GRCGYC	2 cut(s)	243, 255
Hin1II	CATG	9 cut(s)	187, 301, 740, 1036, 1271, 1291, 1678, 1751, 1962
Hin6I	GCGC	3 cut(s)	255, 504, 1116
HinP1I	GCGC	3 cut(s)	255, 504, 1116
HincII	GTYRAC	3 cut(s)	630, 1417, 1671
HindII	GTYRAC	3 cut(s)	630, 1417, 1671
HindIII	AAGCTT	1 cut(s)	1017
HinfI	GANTC	9 cut(s)	383, 534, 626, 826, 1139, 1453, 1484, 1744, 1921
HpaI	GTTAAC	1 cut(s)	1417
HpaII	CCGG	5 cut(s)	378, 558, 581, 1067, 1479
HphI	GGTGA	7 cut(s)	392, 595, 638, 1177, 1596, 1646, 1930
Hpy166II	GTNNAC	3 cut(s)	630, 1417, 1671
Hpy188I	TCNGA	6 cut(s)	696, 891, 977, 1129, 1386, 1710
Hpy8I	GTNNAC	3 cut(s)	630, 1417, 1671
HpyAV	CCTTC	5 cut(s)	109, 285, 407, 1285, 1888
HpyCH4III	ACNGT	6 cut(s)	219, 526, 569, 700, 1192, 1732
HpyCH4IV	ACGT	5 cut(s)	243, 363, 742, 1419, 1665
HpyCH4V	TGCA	9 cut(s)	35, 359, 447, 740, 908, 983, 1151, 1553, 1958
HpyF10VI	GCNNNNNNNGC	9 cut(s)	467, 486, 495, 902, 914, 995, 1115, 1687, 1886
HpyF3I	CTNAG	7 cut(s)	536, 683, 776, 898, 1128, 1371, 1593
HpySE526I	ACGT	5 cut(s)	243, 363, 742, 1419, 1665
Hsp92I	GRCGYC	2 cut(s)	243, 255
Hsp92II	CATG	9 cut(s)	187, 301, 740, 1036, 1271, 1291, 1678, 1751, 1962
HspAI	GCGC	3 cut(s)	255, 504, 1116
KasI	GGCGCC	1 cut(s)	254
Ksp22I	TGATCA	1 cut(s)	1767
KspAI	GTTAAC	1 cut(s)	1417
Kzo9I	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
LmnI	GCTCC	4 cut(s)	494, 1574, 1641, 1886
LweI	GCATC	6 cut(s)	279, 507, 985, 1138, 1562, 1789
MaeII	ACGT	5 cut(s)	243, 363, 742, 1419, 1665
MaeIII	GTNAC	6 cut(s)	239, 380, 514, 660, 700, 812
MalI	GATC	6 cut(s)	296, 395, 405, 752, 1622, 1769
MboI	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
MboII	GAAGA	5 cut(s)	94, 635, 1453, 1862, 1916
MhlI	GDGCHC	1 cut(s)	456
Mly113I	GGCGCC	1 cut(s)	255
MlyI	GAGTC	5 cut(s)	377, 528, 635, 1148, 1462
MmeI	TCCRAC	3 cut(s)	577, 1453, 1552
Mph1103I	ATGCAT	2 cut(s)	1153, 1555
MroXI	GAANNNNTTC	2 cut(s)	98, 1295
MseI	TTAA	4 cut(s)	132, 153, 881, 1416
MslI	CAYNNNNRTG	3 cut(s)	11, 1232, 1916
MspI	CCGG	5 cut(s)	378, 558, 581, 1067, 1479
MspR9I	CCNGG	7 cut(s)	378, 423, 559, 1480, 1489, 1521, 1541
MvaI	CCWGG	4 cut(s)	423, 1489, 1521, 1541
MvnI	CGCG	2 cut(s)	506, 1248
MwoI	GCNNNNNNNGC	9 cut(s)	467, 486, 495, 902, 914, 995, 1115, 1687, 1886
NarI	GGCGCC	1 cut(s)	255
NciI	CCSGG	3 cut(s)	378, 559, 1480
NcoI	CCATGG	2 cut(s)	183, 1032
NdeII	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
NheI	GCTAGC	1 cut(s)	1109
NlaIII	CATG	9 cut(s)	187, 301, 740, 1036, 1271, 1291, 1678, 1751, 1962
NlaIV	GGNNCC	5 cut(s)	256, 967, 1279, 1576, 1888
NmeAIII	GCCGAG	1 cut(s)	313
NmuCI	GTSAC	3 cut(s)	239, 380, 700
NsiI	ATGCAT	2 cut(s)	1153, 1555
NspI	RCATGY	2 cut(s)	740, 1962
PaeI	GCATGC	1 cut(s)	1962
PagI	TCATGA	2 cut(s)	1287, 1747
PceI	AGGCCT	1 cut(s)	1598
PcsI	WCGNNNNNNNCGW	1 cut(s)	1239
PdmI	GAANNNNTTC	2 cut(s)	98, 1295
PfeI	GAWTC	4 cut(s)	826, 1484, 1744, 1921
PinAI	ACCGGT	1 cut(s)	580
PleI	GAGTC	5 cut(s)	377, 528, 634, 1147, 1461
PluTI	GGCGCC	1 cut(s)	258
PpsI	GAGTC	5 cut(s)	377, 528, 634, 1147, 1461
PsiI	TTATAA	1 cut(s)	1253
Psp124BI	GAGCTC	1 cut(s)	456
Psp1406I	AACGTT	1 cut(s)	1419
Psp6I	CCWGG	4 cut(s)	421, 1487, 1519, 1539
PspEI	GGTNACC	1 cut(s)	660
PspGI	CCWGG	4 cut(s)	421, 1487, 1519, 1539
PspN4I	GGNNCC	5 cut(s)	256, 967, 1279, 1576, 1888
PspPI	GGNCC	5 cut(s)	602, 965, 1166, 1278, 1754
PsrI	GAACNNNNNNTAC	2 cut(s)	1766, 1798
PstI	CTGCAG	1 cut(s)	910
PstNI	CAGNNNCTG	1 cut(s)	1883
RsaI	GTAC	2 cut(s)	566, 1093
RsaNI	GTAC	2 cut(s)	565, 1092
RseI	CAYNNNNRTG	3 cut(s)	11, 1232, 1916
SacI	GAGCTC	1 cut(s)	456
SalI	GTCGAC	2 cut(s)	628, 1669
SaqAI	TTAA	4 cut(s)	132, 153, 881, 1416
Sau3AI	GATC	6 cut(s)	294, 393, 403, 750, 1620, 1767
Sau96I	GGNCC	5 cut(s)	602, 965, 1166, 1278, 1754
SchI	GAGTC	5 cut(s)	377, 528, 635, 1148, 1462
ScrFI	CCNGG	7 cut(s)	378, 423, 559, 1480, 1489, 1521, 1541
SduI	GDGCHC	1 cut(s)	456
SfaNI	GCATC	6 cut(s)	279, 507, 985, 1138, 1562, 1789
SfcI	CTRYAG	1 cut(s)	906
SfoI	GGCGCC	1 cut(s)	256
SinI	GGWCC	5 cut(s)	602, 965, 1166, 1278, 1754
SmiMI	CAYNNNNRTG	3 cut(s)	11, 1232, 1916
SpeI	ACTAGT	1 cut(s)	340
SphI	GCATGC	1 cut(s)	1962
SseBI	AGGCCT	1 cut(s)	1598
SsiI	CCGC	7 cut(s)	531, 1143, 1246, 1517, 1843, 1962, 1990
SspDI	GGCGCC	1 cut(s)	254
SspI	AATATT	2 cut(s)	436, 640
SstI	GAGCTC	1 cut(s)	456
StuI	AGGCCT	1 cut(s)	1598
StyD4I	CCNGG	7 cut(s)	376, 421, 557, 1478, 1487, 1519, 1539
StyI	CCWWGG	3 cut(s)	183, 1032, 1836
TaaI	ACNGT	6 cut(s)	219, 526, 569, 700, 1192, 1732
TaiI	ACGT	5 cut(s)	246, 366, 745, 1422, 1668
TaqI	TCGA	7 cut(s)	139, 629, 753, 1242, 1451, 1556, 1670
TauI	GCSGC	2 cut(s)	1519, 1992
TfiI	GAWTC	4 cut(s)	826, 1484, 1744, 1921
Tru1I	TTAA	4 cut(s)	132, 153, 881, 1416
Tru9I	TTAA	4 cut(s)	132, 153, 881, 1416
TscAI	CASTG	6 cut(s)	574, 915, 948, 1195, 1535, 1792
TseFI	GTSAC	3 cut(s)	239, 380, 700
Tsp45I	GTSAC	3 cut(s)	239, 380, 700
TspDTI	ATGAA	7 cut(s)	219, 855, 1284, 1304, 1727, 1736, 1900
TspGWI	ACGGA	1 cut(s)	1270
TspRI	CASTG	6 cut(s)	574, 915, 948, 1195, 1535, 1792
VpaK11BI	GGWCC	5 cut(s)	602, 965, 1166, 1278, 1754
XagI	CCTNNNNNAGG	1 cut(s)	935
XapI	RAATTY	3 cut(s)	574, 607, 1271
XbaI	TCTAGA	1 cut(s)	400
XceI	RCATGY	2 cut(s)	740, 1962
XmiI	GTMKAC	2 cut(s)	629, 1670
XmnI	GAANNNNTTC	2 cut(s)	98, 1295
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	2 cut(s)	1153, 1555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.