Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

39151861 .. 39155135

Gene Structure 3275 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00417420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGACATAAATATGGTGTCCTCAATTCCACTCCGACTCCCAATTCCCACTGCAACCAATATCTCTCGGTTACTAAAACAAAAAACCAAAACCAATGTCTCATTCTGGAATACAAATCATTTCAGTACAAAACCCCAATTTCTTAGTGTCAGTTCTAAATATGTTCCTCTCACGGACATGAAGACTGCAAATAATGGAGCACTCGCGGTCGACGTGACAGGTACGCTGGCTAGCAACAACGATGTCGTAGTGGGCGAAAGCCCACAAAAAGGCGCCATGACCAACTTGTCCGAGCCCACACTGGGAGGTTACATTGCTCGTCGGCTCGTCCAACTCGGTGTCACCGACGTGTTCTCCGTACCCGGTGACTTTAACCTCACCCTCCTCGACTATCTCGTTGCAGAGCCAGGGCTTAACAATATTGGCTGCTGCAATGAGCTCAATGCAGGCTATGCTGCTGATGGCTACGCGCGCTCTCGTGGCGTCGGGGCGTGTGTGGTTACTTTCACTGTGGGAGGGCTCAGTGTCCTCAATGCCATAGCTGGAGCGTATAGTGATGATTTGCCGGTGATTTGTATAGTGGGAGGACCCAACTCCAATGACTATGGAACCAACAGGATTCTTCACCATACTATCGGGTCACCGGATTTTACCCAAGAGCTTAGATGCTTTCAGACTGTCACTTGTTATCAGGCTGTGATAAACAATCTTGAAGATGCACATCTAAAGATCGATACAGCAATTTCAACAGCATTGAAAGAAAGCAAGCCTGTGTATATCAGCATAAGTTGTAACTTGCTTGGGATCCCTCATCCTAGTTTTAGCTGTAAGCCTATTCCAATTTCATTGCCTCCCAGATTAAGTAATCAAATGGCCTTAGAAGCTGCAGTGGAGGCAACTGCAGAGTTCTTGGACAATGCAGTGAAACCAATTATGATAGGTGGCCCCGAACTCCGAGTAGCAAAAGCAGAGGATGCATTCGTCCAGCTAGCTGATGCTTGCGGCTATGCTGTAGCTGTGTTGCCATCGGCAAAGGGGCTGGTGCCGGAATACCACCCCCATTTCATTGGGACATATTGGGGTGTCATCAGCTCTCCCTTCTGTGCCGAGATTGTGGAGTCAGCAGACGCATACTTATGTGCTGGACCAGTTGTCAATGACTATACGTCGGTTGGGTACTCAGCCCTTGTCAAGAAGGAGAAGTCAATTGTTGTGAAGCCGGATCGAGTGATCATAGGTAACGGGCCCGAATTCGGTTTTATTATGATGGAGGATTTTCTCATAGCACTATCAAAAAGACTCAAACGCAATACCACTGCTTATGAGAACTACAACATGATATTTGTTCCTGATGGGCAGTCTCTGCTCAAATGTGATCCCAAAGAGCCTTTGAGGGTTAATGTCATGTTCAAACACGTACAGAATATGTTGACAAGTCAAACAGCTGTGATTGCTGAGACTGGGGACTCATGGTTTAACTGCCAGAAACTGAGATTGCCGCGTGGATGCGGGTATGAGTTCCAAATGCAATATGGATCAATTGGTTGGTCAGTTGGATCAACTCTTGGGTATGCTCAGGCTGTACTGGAGAAGCGAGTCATTTCTTTCATTGGTGATGGGAGCTTCCAGGTGACTGCTCAAGAAGTGTCAACGATGATTCGAAATGGGCAGAGGAACATAATTTTCCTTATAAACAATGGTGGATATACCATTGAAGTGAAGATCCATGATGGACCATACAATGTAATCAAAAACTGGAACTATACTGGACTGGTTGATTCCATCCACAATGGGGAGGGAAAATGCTGGACAACCAAGGTGCGTTCTGAAGAGGAGCTGATTGAAGCAATTGATGCTGCAACTGGAGACAAGAAGGATTGCTTATGCTTCATCGAGGTGATTGTTCATAGGGACGACAGCAGCAAGGAGCTACTTGAGTGGGGCTCTAGGATCGCTGCTGCCGGTAACCGCCCACCTGAGGCTTACCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

663

Amino Acids

72.06

Weight (kDa)

5.59

Isoelectric Point (pI)

28.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	100 - 209	9.9e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	297 - 414	9.5e-21	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	508 - 633	3.3e-13	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1691
AasI	GACNNNNNNGTC	1 cut(s)	286
AccB1I	GGYRCC	2 cut(s)	272, 1042
AccI	GTMKAC	1 cut(s)	210
AccII	CGCG	4 cut(s)	206, 470, 472, 1501
AciI	CCGC	5 cut(s)	206, 1002, 1499, 1509, 1969
AclWI	GGATC	8 cut(s)	799, 812, 1230, 1370, 1543, 1564, 1717, 1958
AcsI	RAATTY	1 cut(s)	1250
AcuI	CTGAAG	1 cut(s)	1848
AcyI	GRCGYC	2 cut(s)	273, 483
AfaI	GTAC	6 cut(s)	127, 223, 360, 1178, 1419, 1584
AfiI	CCNNNNNNNGG	5 cut(s)	269, 302, 1253, 1792, 1978
AflIII	ACRYGT	2 cut(s)	348, 1414
AgsI	TTSAA	6 cut(s)	713, 747, 757, 1411, 1715, 1844
AhdI	GACNNNNNGTC	2 cut(s)	1151, 1165
AjiI	CACGTC	2 cut(s)	214, 349
AjnI	CCWGG	2 cut(s)	406, 1626
AjuI	GAANNNNNNNTTGG	2 cut(s)	86, 118
AleI	CACNNNNGTG	1 cut(s)	347
Alw21I	GWGCWC	2 cut(s)	202, 441
Alw26I	GTCTC	4 cut(s)	103, 1365, 1451, 1860
AlwI	GGATC	8 cut(s)	799, 812, 1230, 1370, 1543, 1564, 1717, 1958
AlwNI	CAGNNNCTG	2 cut(s)	1363, 1489
AoxI	GGCC	3 cut(s)	873, 943, 1244
ApaI	GGGCCC	1 cut(s)	1248
ApeKI	GCWGC	8 cut(s)	426, 429, 455, 884, 1856, 1920, 1955, 1958
ApoI	RAATTY	1 cut(s)	1250
ArsI	GACNNNNNNTTYG	2 cut(s)	81, 113
AspLEI	GCGC	3 cut(s)	275, 472, 474
AspS9I	GGNCC	6 cut(s)	587, 944, 1145, 1244, 1245, 1733
AsuC2I	CCSGG	1 cut(s)	363
AsuHPI	GGTGA	9 cut(s)	334, 370, 377, 580, 617, 633, 1625, 1642, 1909
AsuII	TTCGAA	1 cut(s)	1660
AsuNHI	GCTAGC	2 cut(s)	230, 988
AvaII	GGWCC	3 cut(s)	587, 1145, 1733
AxyI	CCTNAGG	1 cut(s)	1977
BaeGI	GKGCMC	1 cut(s)	1248
BamHI	GGATCC	1 cut(s)	804
BanI	GGYRCC	2 cut(s)	272, 1042
BanII	GRGCYC	5 cut(s)	297, 441, 522, 1248, 1946
BauI	CACGAG	1 cut(s)	477
BbsI	GAAGAC	1 cut(s)	188
Bbv12I	GWGCWC	2 cut(s)	202, 441
BbvI	GCAGC	8 cut(s)	413, 416, 442, 871, 1843, 1932, 1942, 1945
BccI	CCATC	7 cut(s)	455, 1033, 1261, 1346, 1610, 1724, 1790
BciT130I	CCWGG	2 cut(s)	408, 1628
BclI	TGATCA	1 cut(s)	1230
BcnI	CCSGG	1 cut(s)	363
BcoDI	GTCTC	4 cut(s)	103, 1365, 1451, 1860
BfaI	CTAG	4 cut(s)	231, 816, 989, 1947
BfmI	CTRYAG	3 cut(s)	885, 900, 1011
BfoI	RGCGCY	1 cut(s)	276
Bme1390I	CCNGG	3 cut(s)	363, 408, 1628
Bme18I	GGWCC	3 cut(s)	587, 1145, 1733
BmeRI	GACNNNNNGTC	2 cut(s)	1151, 1165
BmgBI	CACGTC	2 cut(s)	214, 349
BmgT120I	GGNCC	6 cut(s)	587, 944, 1145, 1244, 1245, 1733
BmiI	GGNNCC	7 cut(s)	274, 589, 610, 806, 946, 1044, 1246
BmrFI	CCNGG	3 cut(s)	363, 408, 1628
BmrI	ACTGGG	2 cut(s)	311, 1470
BmsI	GCATC	6 cut(s)	656, 706, 964, 985, 1496, 1843
BmtI	GCTAGC	2 cut(s)	234, 992
BmuI	ACTGGG	2 cut(s)	311, 1470
BpiI	GAAGAC	1 cut(s)	188
BplI	GAGNNNNNCTC	2 cut(s)	1928, 1960
BpmI	CTGGAG	3 cut(s)	564, 1607, 1884
Bpu10I	CCTNAGC	1 cut(s)	1575
Bpu14I	TTCGAA	1 cut(s)	1660
BpuEI	CTTGAG	2 cut(s)	1623, 1955
BpuMI	CCSGG	1 cut(s)	363
Bsa29I	ATCGAT	1 cut(s)	732
BsaAI	YACGTR	1 cut(s)	1417
BsaBI	GATNNNNATC	1 cut(s)	720
BsaHI	GRCGYC	2 cut(s)	273, 483
BsaJI	CCNNGG	2 cut(s)	407, 1815
BsaWI	WCCGGW	1 cut(s)	643
BsaXI	ACNNNNNCTCC	4 cut(s)	21, 51, 338, 368
Bsc4I	CCNNNNNNNGG	5 cut(s)	269, 302, 1253, 1792, 1978
Bse118I	RCCGGY	2 cut(s)	565, 1961
Bse1I	ACTGG	9 cut(s)	306, 1148, 1465, 1590, 1760, 1771, 1776, 1867, 1987
Bse21I	CCTNAGG	1 cut(s)	1977
Bse3DI	GCAATG	3 cut(s)	312, 439, 845
Bse8I	GATNNNNATC	1 cut(s)	720
BseBI	CCWGG	2 cut(s)	408, 1628
BseCI	ATCGAT	1 cut(s)	732
BseDI	CCNNGG	2 cut(s)	407, 1815
BseGI	GGATG	4 cut(s)	811, 979, 1511, 1782
BseJI	GATNNNNATC	1 cut(s)	720
BseLI	CCNNNNNNNGG	5 cut(s)	269, 302, 1253, 1792, 1978
BseMI	GCAATG	3 cut(s)	312, 439, 845
BseMII	CTCAG	6 cut(s)	535, 1194, 1446, 1481, 1589, 1968
BseNI	ACTGG	9 cut(s)	306, 1148, 1465, 1590, 1760, 1771, 1776, 1867, 1987
BsePI	GCGCGC	1 cut(s)	470
BseRI	GAGGAG	2 cut(s)	374, 1847
BseSI	GKGCMC	1 cut(s)	1248
BseXI	GCAGC	8 cut(s)	413, 416, 442, 871, 1843, 1932, 1942, 1945
Bsh1236I	CGCG	4 cut(s)	206, 470, 472, 1501
Bsh1285I	CGRYCG	1 cut(s)	210
BshFI	GGCC	3 cut(s)	875, 945, 1246
BshNI	GGYRCC	2 cut(s)	272, 1042
BshVI	ATCGAT	1 cut(s)	732
BsiEI	CGRYCG	1 cut(s)	210
BsiHKAI	GWGCWC	2 cut(s)	202, 441
BsiSI	CCGG	6 cut(s)	363, 566, 644, 1046, 1220, 1962
BslFI	GGGAC	3 cut(s)	1084, 1478, 1925
BslI	CCNNNNNNNGG	5 cut(s)	269, 302, 1253, 1792, 1978
BsmAI	GTCTC	4 cut(s)	103, 1365, 1451, 1860
BsmFI	GGGAC	3 cut(s)	1084, 1478, 1925
BsmI	GAATGC	1 cut(s)	977
BsnI	GGCC	3 cut(s)	875, 945, 1246
Bsp119I	TTCGAA	1 cut(s)	1660
Bsp120I	GGGCCC	1 cut(s)	1244
Bsp1286I	GDGCHC	6 cut(s)	202, 297, 441, 522, 1248, 1946
Bsp143I	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
BspACI	CCGC	5 cut(s)	206, 1002, 1499, 1509, 1969
BspANI	GGCC	3 cut(s)	875, 945, 1246
BspCNI	CTCAG	6 cut(s)	534, 1193, 1447, 1482, 1588, 1969
BspDI	ATCGAT	1 cut(s)	732
BspFNI	CGCG	4 cut(s)	206, 470, 472, 1501
BspLI	GGNNCC	7 cut(s)	274, 589, 610, 806, 946, 1044, 1246
BspMAI	CTGCAG	2 cut(s)	889, 904
BspOI	GCTAGC	2 cut(s)	234, 992
BspPI	GGATC	8 cut(s)	799, 812, 1230, 1370, 1543, 1564, 1717, 1958
BspT104I	TTCGAA	1 cut(s)	1660
BspT107I	GGYRCC	2 cut(s)	272, 1042
BsrDI	GCAATG	3 cut(s)	312, 439, 845
BsrFI	RCCGGY	2 cut(s)	565, 1961
BsrI	ACTGG	9 cut(s)	306, 1148, 1465, 1590, 1760, 1771, 1776, 1867, 1987
BssAI	RCCGGY	2 cut(s)	565, 1961
BssECI	CCNNGG	2 cut(s)	407, 1815
BssHII	GCGCGC	1 cut(s)	470
BssMI	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
BssNI	GRCGYC	2 cut(s)	273, 483
BssSI	CACGAG	1 cut(s)	477
BssT1I	CCWWGG	1 cut(s)	1815
Bst2BI	CACGAG	1 cut(s)	477
Bst2UI	CCWGG	2 cut(s)	408, 1628
Bst4CI	ACNGT	2 cut(s)	511, 679
Bst6I	CTCTTC	1 cut(s)	1824
BstACI	GRCGYC	2 cut(s)	273, 483
BstAPI	GCANNNNNTGC	4 cut(s)	452, 974, 1363, 1853
BstBAI	YACGTR	1 cut(s)	1417
BstBI	TTCGAA	1 cut(s)	1660
BstC8I	GCNNGC	7 cut(s)	228, 232, 448, 472, 767, 990, 1000
BstDEI	CTNAG	9 cut(s)	143, 521, 662, 877, 1180, 1455, 1490, 1575, 1977
BstEII	GGTNACC	2 cut(s)	639, 1964
BstF5I	GGATG	4 cut(s)	811, 979, 1511, 1782
BstFNI	CGCG	4 cut(s)	206, 470, 472, 1501
BstH2I	RGCGCY	1 cut(s)	276
BstHHI	GCGC	3 cut(s)	275, 472, 474
BstKTI	GATC	9 cut(s)	732, 807, 1225, 1233, 1378, 1538, 1559, 1725, 1953
BstMAI	GTCTC	4 cut(s)	103, 1365, 1451, 1860
BstMBI	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
BstMCI	CGRYCG	1 cut(s)	210
BstMWI	GCNNNNNNNGC	9 cut(s)	452, 471, 480, 881, 893, 974, 1363, 1451, 1853
BstNI	CCWGG	2 cut(s)	408, 1628
BstPI	GGTNACC	2 cut(s)	639, 1964
BstSCI	CCNGG	3 cut(s)	361, 406, 1626
BstSFI	CTRYAG	3 cut(s)	885, 900, 1011
BstSLI	GKGCMC	1 cut(s)	1248
BstUI	CGCG	4 cut(s)	206, 470, 472, 1501
BstV1I	GCAGC	8 cut(s)	413, 416, 442, 871, 1843, 1932, 1942, 1945
BstV2I	GAAGAC	1 cut(s)	188
BstX2I	RGATCY	2 cut(s)	804, 1722
BstXI	CCANNNNNNTGG	1 cut(s)	1067
BstYI	RGATCY	2 cut(s)	804, 1722
Bsu15I	ATCGAT	1 cut(s)	732
Bsu36I	CCTNAGG	1 cut(s)	1977
BsuRI	GGCC	3 cut(s)	875, 945, 1246
BsuTUI	ATCGAT	1 cut(s)	732
BtrI	CACGTC	2 cut(s)	214, 349
BtsCI	GGATG	4 cut(s)	811, 979, 1511, 1782
BtsI	GCAGTG	4 cut(s)	48, 894, 927, 1314
BtsIMutI	CAGTG	7 cut(s)	48, 299, 507, 529, 894, 927, 1314
Cac8I	GCNNGC	7 cut(s)	228, 232, 448, 472, 767, 990, 1000
CaiI	CAGNNNCTG	2 cut(s)	1363, 1489
CfoI	GCGC	3 cut(s)	275, 472, 474
Cfr10I	RCCGGY	2 cut(s)	565, 1961
Cfr13I	GGNCC	6 cut(s)	587, 944, 1145, 1244, 1245, 1733
ClaI	ATCGAT	1 cut(s)	732
CseI	GACGC	2 cut(s)	472, 1136
Csp6I	GTAC	6 cut(s)	126, 222, 359, 1177, 1418, 1583
CviAII	CATG	6 cut(s)	178, 277, 1336, 1405, 1470, 1727
CviQI	GTAC	6 cut(s)	126, 222, 359, 1177, 1418, 1583
DdeI	CTNAG	9 cut(s)	143, 521, 662, 877, 1180, 1455, 1490, 1575, 1977
DinI	GGCGCC	1 cut(s)	274
DpnI	GATC	9 cut(s)	731, 806, 1224, 1232, 1377, 1537, 1558, 1724, 1952
DpnII	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
DrdI	GACNNNNNNGTC	1 cut(s)	286
DriI	GACNNNNNGTC	2 cut(s)	1151, 1165
DseDI	GACNNNNNNGTC	1 cut(s)	286
Eam1104I	CTCTTC	1 cut(s)	1824
Eam1105I	GACNNNNNGTC	2 cut(s)	1151, 1165
EarI	CTCTTC	1 cut(s)	1824
Ecl136II	GAGCTC	1 cut(s)	439
Eco130I	CCWWGG	1 cut(s)	1815
Eco24I	GRGCYC	5 cut(s)	297, 441, 522, 1248, 1946
Eco47I	GGWCC	3 cut(s)	587, 1145, 1733
Eco53kI	GAGCTC	1 cut(s)	439
Eco57I	CTGAAG	1 cut(s)	1848
Eco81I	CCTNAGG	1 cut(s)	1977
Eco91I	GGTNACC	2 cut(s)	639, 1964
EcoICRI	GAGCTC	1 cut(s)	439
EcoO109I	RGGNCCY	1 cut(s)	587
EcoO65I	GGTNACC	2 cut(s)	639, 1964
EcoRI	GAATTC	1 cut(s)	1250
EcoRII	CCWGG	2 cut(s)	406, 1626
EcoT14I	CCWWGG	1 cut(s)	1815
EcoT22I	ATGCAT	1 cut(s)	979
EcoT38I	GRGCYC	5 cut(s)	297, 441, 522, 1248, 1946
EgeI	GGCGCC	1 cut(s)	274
EheI	GGCGCC	1 cut(s)	274
ErhI	CCWWGG	1 cut(s)	1815
FaeI	CATG	6 cut(s)	181, 280, 1339, 1408, 1473, 1730
FalI	AAGNNNNNCTT	2 cut(s)	1865, 1897
FaqI	GGGAC	3 cut(s)	1084, 1478, 1925
FatI	CATG	6 cut(s)	177, 276, 1335, 1404, 1469, 1726
FauI	CCCGC	1 cut(s)	1502
FbaI	TGATCA	1 cut(s)	1230
FblI	GTMKAC	1 cut(s)	210
FokI	GGATG	4 cut(s)	798, 986, 1518, 1769
FriOI	GRGCYC	5 cut(s)	297, 441, 522, 1248, 1946
FspBI	CTAG	4 cut(s)	231, 816, 989, 1947
GlaI	GCGC	3 cut(s)	274, 471, 473
GsuI	CTGGAG	3 cut(s)	564, 1607, 1884
HaeII	RGCGCY	1 cut(s)	276
HaeIII	GGCC	3 cut(s)	875, 945, 1246
HapII	CCGG	6 cut(s)	363, 566, 644, 1046, 1220, 1962
HgaI	GACGC	2 cut(s)	472, 1136
HhaI	GCGC	3 cut(s)	275, 472, 474
Hin1I	GRCGYC	2 cut(s)	273, 483
Hin1II	CATG	6 cut(s)	181, 280, 1339, 1408, 1473, 1730
Hin6I	GCGC	3 cut(s)	273, 470, 472
HinP1I	GCGC	3 cut(s)	273, 470, 472
HincII	GTYRAC	3 cut(s)	211, 1431, 1650
HindII	GTYRAC	3 cut(s)	211, 1431, 1650
HinfI	GANTC	8 cut(s)	36, 619, 1118, 1299, 1466, 1596, 1657, 1778
HpaII	CCGG	6 cut(s)	363, 566, 644, 1046, 1220, 1962
HphI	GGTGA	9 cut(s)	334, 370, 377, 580, 617, 633, 1625, 1642, 1909
Hpy166II	GTNNAC	3 cut(s)	211, 1431, 1650
Hpy188I	TCNGA	5 cut(s)	35, 292, 675, 956, 1828
Hpy188III	TCNNGA	5 cut(s)	106, 710, 1192, 1349, 1640
Hpy8I	GTNNAC	3 cut(s)	211, 1431, 1650
Hpy99I	CGWCG	5 cut(s)	215, 324, 350, 488, 1171
HpyAV	CCTTC	3 cut(s)	1108, 1189, 1867
HpyCH4III	ACNGT	2 cut(s)	511, 679
HpyCH4IV	ACGT	4 cut(s)	213, 348, 1166, 1416
HpyF10VI	GCNNNNNNNGC	9 cut(s)	452, 471, 480, 881, 893, 974, 1363, 1451, 1853
HpyF3I	CTNAG	9 cut(s)	143, 521, 662, 877, 1180, 1455, 1490, 1575, 1977
HpySE526I	ACGT	4 cut(s)	213, 348, 1166, 1416
Hsp92I	GRCGYC	2 cut(s)	273, 483
Hsp92II	CATG	6 cut(s)	181, 280, 1339, 1408, 1473, 1730
HspAI	GCGC	3 cut(s)	273, 470, 472
KasI	GGCGCC	1 cut(s)	272
Ksp22I	TGATCA	1 cut(s)	1230
Kzo9I	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
LmnI	GCTCC	5 cut(s)	197, 545, 1620, 1834, 1927
Lsp1109I	GCAGC	8 cut(s)	413, 416, 442, 871, 1843, 1932, 1942, 1945
LweI	GCATC	6 cut(s)	656, 706, 964, 985, 1496, 1843
MaeI	CTAG	4 cut(s)	231, 816, 989, 1947
MaeII	ACGT	4 cut(s)	213, 348, 1166, 1416
MalI	GATC	9 cut(s)	731, 806, 1224, 1232, 1377, 1537, 1558, 1724, 1952
MboI	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
MboII	GAAGA	5 cut(s)	193, 614, 725, 1732, 1841
MfeI	CAATTG	3 cut(s)	1206, 1539, 1848
MflI	RGATCY	2 cut(s)	804, 1722
MhlI	GDGCHC	6 cut(s)	202, 297, 441, 522, 1248, 1946
Mly113I	GGCGCC	1 cut(s)	273
MlyI	GAGTC	5 cut(s)	30, 1127, 1293, 1460, 1605
MmeI	TCCRAC	3 cut(s)	58, 355, 1534
Mph1103I	ATGCAT	1 cut(s)	979
MseI	TTAA	5 cut(s)	372, 414, 860, 1398, 1476
MslI	CAYNNNNRTG	6 cut(s)	11, 176, 347, 1135, 1715, 1895
MspA1I	CMGCKG	1 cut(s)	1445
MspI	CCGG	6 cut(s)	363, 566, 644, 1046, 1220, 1962
MspR9I	CCNGG	3 cut(s)	363, 408, 1628
MunI	CAATTG	3 cut(s)	1206, 1539, 1848
Mva1269I	GAATGC	1 cut(s)	977
MvaI	CCWGG	2 cut(s)	408, 1628
MvnI	CGCG	4 cut(s)	206, 470, 472, 1501
MwoI	GCNNNNNNNGC	9 cut(s)	452, 471, 480, 881, 893, 974, 1363, 1451, 1853
NarI	GGCGCC	1 cut(s)	273
NciI	CCSGG	1 cut(s)	363
NdeII	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
NheI	GCTAGC	2 cut(s)	230, 988
NlaIII	CATG	6 cut(s)	181, 280, 1339, 1408, 1473, 1730
NlaIV	GGNNCC	7 cut(s)	274, 589, 610, 806, 946, 1044, 1246
NmeAIII	GCCGAG	1 cut(s)	1132
NmuCI	GTSAC	6 cut(s)	214, 340, 365, 639, 679, 1630
NsiI	ATGCAT	1 cut(s)	979
NspV	TTCGAA	1 cut(s)	1660
OliI	CACNNNNGTG	1 cut(s)	347
PauI	GCGCGC	1 cut(s)	470
PcsI	WCGNNNNNNNCGW	5 cut(s)	210, 252, 342, 354, 393
PctI	GAATGC	1 cut(s)	977
PfeI	GAWTC	3 cut(s)	619, 1657, 1778
PleI	GAGTC	5 cut(s)	30, 1126, 1293, 1460, 1604
PluTI	GGCGCC	1 cut(s)	276
PpsI	GAGTC	5 cut(s)	30, 1126, 1293, 1460, 1604
Ppu21I	YACGTR	1 cut(s)	1417
PpuMI	RGGWCCY	1 cut(s)	587
PsiI	TTATAA	1 cut(s)	1691
Psp124BI	GAGCTC	1 cut(s)	441
Psp5II	RGGWCCY	1 cut(s)	587
Psp6I	CCWGG	2 cut(s)	406, 1626
PspEI	GGTNACC	2 cut(s)	639, 1964
PspGI	CCWGG	2 cut(s)	406, 1626
PspN4I	GGNNCC	7 cut(s)	274, 589, 610, 806, 946, 1044, 1246
PspOMI	GGGCCC	1 cut(s)	1244
PspPI	GGNCC	6 cut(s)	587, 944, 1145, 1244, 1245, 1733
PspPPI	RGGWCCY	1 cut(s)	587
PstI	CTGCAG	2 cut(s)	889, 904
PstNI	CAGNNNCTG	2 cut(s)	1363, 1489
PsuI	RGATCY	2 cut(s)	804, 1722
PteI	GCGCGC	1 cut(s)	470
PvuII	CAGCTG	1 cut(s)	1445
RsaI	GTAC	6 cut(s)	127, 223, 360, 1178, 1419, 1584
RsaNI	GTAC	6 cut(s)	126, 222, 359, 1177, 1418, 1583
RseI	CAYNNNNRTG	6 cut(s)	11, 176, 347, 1135, 1715, 1895
SacI	GAGCTC	1 cut(s)	441
SalI	GTCGAC	1 cut(s)	209
SaqAI	TTAA	5 cut(s)	372, 414, 860, 1398, 1476
Sau3AI	GATC	9 cut(s)	729, 804, 1222, 1230, 1375, 1535, 1556, 1722, 1950
Sau96I	GGNCC	6 cut(s)	587, 944, 1145, 1244, 1245, 1733
SchI	GAGTC	5 cut(s)	30, 1127, 1293, 1460, 1605
ScrFI	CCNGG	3 cut(s)	363, 408, 1628
SduI	GDGCHC	6 cut(s)	202, 297, 441, 522, 1248, 1946
SfaNI	GCATC	6 cut(s)	656, 706, 964, 985, 1496, 1843
SfcI	CTRYAG	3 cut(s)	885, 900, 1011
SfoI	GGCGCC	1 cut(s)	274
SfuI	TTCGAA	1 cut(s)	1660
SinI	GGWCC	3 cut(s)	587, 1145, 1733
SmiMI	CAYNNNNRTG	6 cut(s)	11, 176, 347, 1135, 1715, 1895
SmlI	CTYRAG	2 cut(s)	1638, 1934
SmoI	CTYRAG	2 cut(s)	1638, 1934
SsiI	CCGC	5 cut(s)	206, 1002, 1499, 1509, 1969
SspDI	GGCGCC	1 cut(s)	272
SspI	AATATT	1 cut(s)	421
SspMI	CTAG	4 cut(s)	231, 816, 989, 1947
SstI	GAGCTC	1 cut(s)	441
StyD4I	CCNGG	3 cut(s)	361, 406, 1626
StyI	CCWWGG	1 cut(s)	1815
TaaI	ACNGT	2 cut(s)	511, 679
TaiI	ACGT	4 cut(s)	216, 351, 1169, 1419
TaqI	TCGA	6 cut(s)	210, 387, 732, 1225, 1660, 1893
TatI	WGTACW	2 cut(s)	125, 1582
TauI	GCSGC	2 cut(s)	1005, 1501
TfiI	GAWTC	3 cut(s)	619, 1657, 1778
Tru1I	TTAA	5 cut(s)	372, 414, 860, 1398, 1476
Tru9I	TTAA	5 cut(s)	372, 414, 860, 1398, 1476
TscAI	CASTG	7 cut(s)	55, 306, 514, 529, 894, 927, 1321
TseFI	GTSAC	6 cut(s)	214, 340, 365, 639, 679, 1630
TseI	GCWGC	8 cut(s)	426, 429, 455, 884, 1856, 1920, 1955, 1958
Tsp45I	GTSAC	6 cut(s)	214, 340, 365, 639, 679, 1630
TspDTI	ATGAA	6 cut(s)	194, 834, 1054, 1597, 1879, 1895
TspGWI	ACGGA	2 cut(s)	188, 346
TspRI	CASTG	7 cut(s)	55, 306, 514, 529, 894, 927, 1321
VpaK11BI	GGWCC	3 cut(s)	587, 1145, 1733
XapI	RAATTY	1 cut(s)	1250
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1529
XmiI	GTMKAC	1 cut(s)	210
XspI	CTAG	4 cut(s)	231, 816, 989, 1947
Zsp2I	ATGCAT	1 cut(s)	979

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.