Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003546

Physical Location & Seq

Genomic Coordinates Reverse (-)

16851972 .. 16853035

Gene Structure 1064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G35480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 603 bp

ATGACTCGCTCCTCTAGATTCCTTGGTACGGCGTCGCCTCCACCACCGGAAGAAATCCTCGCCGCCGAAACCGACATGGTTGTGATTCTCTCAGCACTTCTCTGCGCTCTCGTATGCGTAGCCGGTTTAGCCGCCGTAGCTCGCTGCGCTTGGCTCCGCCGTCTCACCGGAGTTAATCCCGCCGCCGTCGGAGAAGCTCCACCGCCTAACAAAGGCCTCAAGAAAAAAGCACTTCAAGCTCTCCCTAAATCAACTTACACCGCCTCTGCCTCTACCGCCGCCGCCGCTGACGATCTACCGTGCTCTTCAGTCGGAGACGGAGATTCATCCACCGAGTGTGCAATATGCATAACGGAATTTTCGGAGGGCGAAGAAATCAGAATCTTACCGCTGTGTAGTCACGCCTTCCACGTGGCGTGTATAGACAAGTGGTTGACTTCACGGTCGTCGTGTCCGTCTTGTCGTCGGATACTGGTGCCGGTGAAGTGTGATAGGTGTGGGCACCATGCTTCGACGGCGGAGACTCAGGTCAAAGATCAACCTCCTCATCACCAACATCCTTCACAGTTCACTTCCGCCATTATTCCTGCTTTTCTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

200

Amino Acids

21.08

Weight (kDa)

7.01

Isoelectric Point (pI)

66.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	112 - 155	1.1e-14	Ring finger domain
zf-RING_11	PF17123	112 - 141	2.2e-11	RING-like zinc finger
zf-C3HC4_2	PF13923	112 - 154	4.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	113 - 154	5.5e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	116 - 155	3.7e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17450 AT4G35480
fragaria_vesca	FvH4_3g20200 FvH4_5g05151
malus_domestica	MD03G1226600.v1.1 MD03G1226700.v1.1 MD06G1176900.v1.1 MD11G1244200.v1.1 MD11G1244300.v1.1 MD11G1244400.v1.1 MD14G1183100.v1.1
prunus_persica	Prupe.4G182700_v2.0.a1 Prupe.4G182800_v2.0.a1 Prupe.5G175600_v2.0.a1 Prupe.6G077100_v2.0.a1
pyrus_communis	pycom06g15700
rosa_chinensis	RchiOBHm_Chr5g0034021 RchiOBHm_Chr7g0193811
rosa_laevigata	RLG00000004243
rosa_multiflora	Rmu_sc0001436.1_g000029
rosa_roxburghii	Rroxscaffold_1G00046210 Rroxscaffold_3G00261810
rosa_rugosa	Rorug07G0012200
rosa_samantha	Rh5AG235200 Rh5BG235900 Rh5CG265700 Rh5CG265800 Rh5DG242400 Rh7AG138800 Rh7BG139100 Rh7CG142800 Rh7DG140400
rosa_wichuraiana	Rw5G021230 Rw5G021450 Rw7G011830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	442
AccB1I	GGYRCC	2 cut(s)	475, 501
AcsI	RAATTY	1 cut(s)	356
AcuI	CTGAAG	1 cut(s)	291
AcvI	CACGTG	1 cut(s)	412
AcyI	GRCGYC	1 cut(s)	32
AdeI	CACNNNGTG	1 cut(s)	336
AfaI	GTAC	1 cut(s)	28
AfiI	CCNNNNNNNGG	2 cut(s)	28, 212
AgsI	TTSAA	1 cut(s)	236
AluBI	AGCT	3 cut(s)	140, 197, 239
AluI	AGCT	3 cut(s)	140, 197, 239
Alw21I	GWGCWC	1 cut(s)	305
Alw26I	GTCTC	3 cut(s)	167, 309, 515
AoxI	GGCC	1 cut(s)	214
ApeKI	GCWGC	1 cut(s)	144
ApoI	RAATTY	1 cut(s)	356
AspLEI	GCGC	2 cut(s)	107, 149
AsuHPI	GGTGA	3 cut(s)	157, 493, 542
BaeGI	GKGCMC	1 cut(s)	504
BanI	GGYRCC	2 cut(s)	475, 501
BbrPI	CACGTG	1 cut(s)	412
Bbv12I	GWGCWC	1 cut(s)	305
BbvI	GCAGC	1 cut(s)	131
BceAI	ACGGC	5 cut(s)	45, 119, 144, 170, 531
BciVI	GTATCC	1 cut(s)	462
BcoDI	GTCTC	3 cut(s)	167, 309, 515
BfaI	CTAG	1 cut(s)	15
BfuI	GTATCC	1 cut(s)	462
BisI	GCNGC	7 cut(s)	63, 132, 145, 183, 279, 282, 285
BlsI	GCNGC	7 cut(s)	64, 133, 146, 184, 280, 283, 286
BmiI	GGNNCC	3 cut(s)	155, 477, 503
BoxI	GACNNNNGTC	1 cut(s)	527
BpuEI	CTTGAG	1 cut(s)	203
BsaAI	YACGTR	1 cut(s)	412
BsaHI	GRCGYC	1 cut(s)	32
BsaJI	CCNNGG	1 cut(s)	22
BsaWI	WCCGGW	2 cut(s)	46, 167
Bsc4I	CCNNNNNNNGG	2 cut(s)	28, 212
Bse118I	RCCGGY	2 cut(s)	122, 478
Bse1I	ACTGG	1 cut(s)	477
BseDI	CCNNGG	1 cut(s)	22
BseGI	GGATG	2 cut(s)	326, 556
BseLI	CCNNNNNNNGG	2 cut(s)	28, 212
BseMII	CTCAG	2 cut(s)	105, 539
BseNI	ACTGG	1 cut(s)	477
BseRI	GAGGAG	1 cut(s)	534
BseSI	GKGCMC	1 cut(s)	504
BseXI	GCAGC	1 cut(s)	131
Bsh1285I	CGRYCG	1 cut(s)	446
BshFI	GGCC	1 cut(s)	216
BshNI	GGYRCC	2 cut(s)	475, 501
BsiEI	CGRYCG	1 cut(s)	446
BsiHKAI	GWGCWC	1 cut(s)	305
BsiSI	CCGG	4 cut(s)	47, 123, 168, 479
BslI	CCNNNNNNNGG	2 cut(s)	28, 212
BsmAI	GTCTC	3 cut(s)	167, 309, 515
BsmBI	CGTCTC	2 cut(s)	167, 309
BsnI	GGCC	1 cut(s)	216
Bsp1286I	GDGCHC	2 cut(s)	305, 504
Bsp143I	GATC	2 cut(s)	292, 535
BspANI	GGCC	1 cut(s)	216
BspCNI	CTCAG	2 cut(s)	104, 538
BspLI	GGNNCC	3 cut(s)	155, 477, 503
BspQI	GCTCTTC	1 cut(s)	310
BspT107I	GGYRCC	2 cut(s)	475, 501
BsrFI	RCCGGY	2 cut(s)	122, 478
BsrI	ACTGG	1 cut(s)	477
BssAI	RCCGGY	2 cut(s)	122, 478
BssECI	CCNNGG	1 cut(s)	22
BssMI	GATC	2 cut(s)	292, 535
BssNI	GRCGYC	1 cut(s)	32
BssT1I	CCWWGG	1 cut(s)	22
Bst4CI	ACNGT	3 cut(s)	300, 444, 567
Bst6I	CTCTTC	1 cut(s)	310
BstACI	GRCGYC	1 cut(s)	32
BstBAI	YACGTR	1 cut(s)	412
BstC8I	GCNNGC	1 cut(s)	142
BstDEI	CTNAG	2 cut(s)	91, 525
BstENI	CCTNNNNNAGG	1 cut(s)	210
BstF5I	GGATG	2 cut(s)	326, 556
BstHHI	GCGC	2 cut(s)	107, 149
BstKTI	GATC	2 cut(s)	295, 538
BstMAI	GTCTC	3 cut(s)	167, 309, 515
BstMBI	GATC	2 cut(s)	292, 535
BstMCI	CGRYCG	1 cut(s)	446
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 137, 146, 236, 275, 284, 515
BstPAI	GACNNNNGTC	1 cut(s)	527
BstSLI	GKGCMC	1 cut(s)	504
BstV1I	GCAGC	1 cut(s)	131
BsuI	GTATCC	1 cut(s)	462
BsuRI	GGCC	1 cut(s)	216
BtsCI	GGATG	2 cut(s)	326, 556
Cac8I	GCNNGC	1 cut(s)	142
CfoI	GCGC	2 cut(s)	107, 149
Cfr10I	RCCGGY	2 cut(s)	122, 478
CseI	GACGC	1 cut(s)	21
Csp6I	GTAC	1 cut(s)	27
CviAII	CATG	2 cut(s)	76, 506
CviJI	RGCY	7 cut(s)	122, 131, 140, 154, 197, 216, 239
CviKI_1	RGCY	7 cut(s)	122, 131, 140, 154, 197, 216, 239
CviQI	GTAC	1 cut(s)	27
DdeI	CTNAG	2 cut(s)	91, 525
DpnI	GATC	2 cut(s)	294, 537
DpnII	GATC	2 cut(s)	292, 535
DraIII	CACNNNGTG	1 cut(s)	336
DrdI	GACNNNNNNGTC	1 cut(s)	442
DseDI	GACNNNNNNGTC	1 cut(s)	442
Eam1104I	CTCTTC	1 cut(s)	310
EarI	CTCTTC	1 cut(s)	310
EciI	GGCGGA	3 cut(s)	146, 533, 565
Eco130I	CCWWGG	1 cut(s)	22
Eco147I	AGGCCT	1 cut(s)	216
Eco57I	CTGAAG	1 cut(s)	291
Eco72I	CACGTG	1 cut(s)	412
EcoNI	CCTNNNNNAGG	1 cut(s)	210
EcoT14I	CCWWGG	1 cut(s)	22
EcoT22I	ATGCAT	1 cut(s)	350
ErhI	CCWWGG	1 cut(s)	22
Esp3I	CGTCTC	2 cut(s)	167, 309
FaeI	CATG	2 cut(s)	79, 509
FaiI	YATR	6 cut(s)	77, 115, 346, 350, 422, 507
FatI	CATG	2 cut(s)	75, 505
FauI	CCCGC	1 cut(s)	187
Fnu4HI	GCNGC	7 cut(s)	63, 132, 145, 183, 279, 282, 285
FokI	GGATG	2 cut(s)	313, 543
Fsp4HI	GCNGC	7 cut(s)	63, 132, 145, 183, 279, 282, 285
FspBI	CTAG	1 cut(s)	15
GlaI	GCGC	2 cut(s)	106, 148
GluI	GCNGC	7 cut(s)	63, 132, 145, 183, 279, 282, 285
HaeIII	GGCC	1 cut(s)	216
HapII	CCGG	4 cut(s)	47, 123, 168, 479
HgaI	GACGC	1 cut(s)	21
HhaI	GCGC	2 cut(s)	107, 149
Hin1I	GRCGYC	1 cut(s)	32
Hin1II	CATG	2 cut(s)	79, 509
Hin6I	GCGC	2 cut(s)	105, 147
HinP1I	GCGC	2 cut(s)	105, 147
HincII	GTYRAC	1 cut(s)	435
HindII	GTYRAC	1 cut(s)	435
HinfI	GANTC	6 cut(s)	4, 18, 85, 323, 381, 523
HpaII	CCGG	4 cut(s)	47, 123, 168, 479
HphI	GGTGA	3 cut(s)	157, 493, 542
Hpy166II	GTNNAC	2 cut(s)	435, 570
Hpy188I	TCNGA	5 cut(s)	191, 314, 364, 380, 468
Hpy188III	TCNNGA	2 cut(s)	15, 220
Hpy8I	GTNNAC	2 cut(s)	435, 570
Hpy99I	CGWCG	5 cut(s)	37, 191, 451, 468, 517
HpyAV	CCTTC	2 cut(s)	415, 570
HpyCH4III	ACNGT	3 cut(s)	300, 444, 567
HpyCH4IV	ACGT	1 cut(s)	411
HpyCH4V	TGCA	2 cut(s)	341, 348
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 137, 146, 236, 275, 284, 515
HpyF3I	CTNAG	2 cut(s)	91, 525
HpySE526I	ACGT	1 cut(s)	411
Hsp92I	GRCGYC	1 cut(s)	32
Hsp92II	CATG	2 cut(s)	79, 509
HspAI	GCGC	2 cut(s)	105, 147
Kzo9I	GATC	2 cut(s)	292, 535
LguI	GCTCTTC	1 cut(s)	310
LmnI	GCTCC	3 cut(s)	14, 159, 202
LpnPI	CCDG	6 cut(s)	60, 136, 181, 458, 492, 512
Lsp1109I	GCAGC	1 cut(s)	131
MaeI	CTAG	1 cut(s)	15
MaeII	ACGT	1 cut(s)	411
MaeIII	GTNAC	1 cut(s)	398
MalI	GATC	2 cut(s)	294, 537
MboI	GATC	2 cut(s)	292, 535
MboII	GAAGA	4 cut(s)	62, 297, 383, 587
MhlI	GDGCHC	2 cut(s)	305, 504
MluCI	AATT	1 cut(s)	356
MlyI	GAGTC	1 cut(s)	517
MmeI	TCCRAC	3 cut(s)	169, 292, 446
MnlI	CCTC	9 cut(s)	22, 48, 68, 227, 274, 280, 358, 552, 555
Mph1103I	ATGCAT	1 cut(s)	350
MseI	TTAA	1 cut(s)	174
MslI	CAYNNNNRTG	1 cut(s)	80
MspA1I	CMGCKG	2 cut(s)	287, 391
MspI	CCGG	4 cut(s)	47, 123, 168, 479
MwoI	GCNNNNNNNGC	7 cut(s)	128, 137, 146, 236, 275, 284, 515
NdeII	GATC	2 cut(s)	292, 535
NlaIII	CATG	2 cut(s)	79, 509
NlaIV	GGNNCC	3 cut(s)	155, 477, 503
NmuCI	GTSAC	1 cut(s)	398
NsiI	ATGCAT	1 cut(s)	350
PceI	AGGCCT	1 cut(s)	216
PciSI	GCTCTTC	1 cut(s)	310
PcsI	WCGNNNNNNNCGW	2 cut(s)	408, 452
PfeI	GAWTC	4 cut(s)	18, 85, 323, 381
PkrI	GCNGC	7 cut(s)	64, 133, 146, 184, 280, 283, 286
PleI	GAGTC	1 cut(s)	517
PmaCI	CACGTG	1 cut(s)	412
PmlI	CACGTG	1 cut(s)	412
PpsI	GAGTC	1 cut(s)	517
Ppu21I	YACGTR	1 cut(s)	412
PshAI	GACNNNNGTC	1 cut(s)	527
PspCI	CACGTG	1 cut(s)	412
PspN4I	GGNNCC	3 cut(s)	155, 477, 503
RsaI	GTAC	1 cut(s)	28
RsaNI	GTAC	1 cut(s)	27
RseI	CAYNNNNRTG	1 cut(s)	80
SapI	GCTCTTC	1 cut(s)	310
SaqAI	TTAA	1 cut(s)	174
SatI	GCNGC	7 cut(s)	63, 132, 145, 183, 279, 282, 285
Sau3AI	GATC	2 cut(s)	292, 535
SchI	GAGTC	1 cut(s)	517
SduI	GDGCHC	2 cut(s)	305, 504
SetI	ASST	7 cut(s)	142, 199, 241, 414, 497, 531, 544
SmiMI	CAYNNNNRTG	1 cut(s)	80
SmlI	CTYRAG	1 cut(s)	218
SmoI	CTYRAG	1 cut(s)	218
Sse9I	AATT	1 cut(s)	356
SseBI	AGGCCT	1 cut(s)	216
SspMI	CTAG	1 cut(s)	15
StuI	AGGCCT	1 cut(s)	216
StyI	CCWWGG	1 cut(s)	22
TaaI	ACNGT	3 cut(s)	300, 444, 567
TaiI	ACGT	1 cut(s)	414
TaqI	TCGA	1 cut(s)	512
TasI	AATT	1 cut(s)	356
TauI	GCSGC	6 cut(s)	65, 134, 185, 281, 284, 287
TfiI	GAWTC	4 cut(s)	18, 85, 323, 381
Tru1I	TTAA	1 cut(s)	174
Tru9I	TTAA	1 cut(s)	174
TseFI	GTSAC	1 cut(s)	398
TseI	GCWGC	1 cut(s)	144
Tsp45I	GTSAC	1 cut(s)	398
TspDTI	ATGAA	1 cut(s)	315
TspGWI	ACGGA	3 cut(s)	333, 368, 444
XagI	CCTNNNNNAGG	1 cut(s)	210
XapI	RAATTY	1 cut(s)	356
XbaI	TCTAGA	1 cut(s)	14
XspI	CTAG	1 cut(s)	15
Zsp2I	ATGCAT	1 cut(s)	350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.